Restriction map for
pFN21AB3324
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoNI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 644 GGTACC G^GTACC
AflIII 1130 ACACGT A^C[GA][CT]GT
AhdI 1632 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1150 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 644 GGTACC G^GTACC
AspEI 1632 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 557 CTCGGG C^[CT]CG[GA]G
AvrII 196 CCTAGG C^CTAGG
BalI 317 TGGCCA TGG^CCA
BanI 644 GGTACC G^G[CT][GA]CC
BcnI 6 CCCGG CC^[GC]GG
BfaI 197 CTAG C^TAG
BglII 977 AGATCT A^GATCT
BlnI 196 CCTAGG C^CTAGG
BsaI 62 GGTCTC GGTCTC(1/5)
BseRI 785 CTCCTC GAGGAG(10/8)
BsgI 1159 CTGCAC GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsoBI 557 CTCGGG C^[CT]CG[GA]G
BspMI 1192 ACCTGC ACCTGC(4/8)
BstAPI 286 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1188 GGTGACC G^GT[ACGT]ACC
BstPI 1188 GGTGACC G^GT[ACGT]ACC
CfoI 137 GCGC GCG^C
DraIII 611 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1088 CCACGG C^C[GA][CT]GG
EagI 8 CGGCCG C^GGCCG
Eam1105I 1632 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoO65I 1188 GGTGACC G^GT[ACGT]ACC
EcoT14I 196 CCTAGG C^C[AT][AT]GG
HhaI 137 GCGC GCG^C
HinP1I 137 GCGC G^CGC
HindIII 679 AAGCTT A^AGCTT
KpnI 644 GGTACC GGTAC^C
MaeI 197 CTAG C^TAG
MaeII 1132 ACGT A^CGT
MluNI 317 TGGCCA TGG^CCA
MscI 317 TGGCCA TGG^CCA
NciI 6 CCCGG CC^[GC]GG
PflMI 127 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1378 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1625 CTGCAG CTGCA^G
SbfI 1624 CCTGCAGG CCTGCA^GG
ScaI 1360 AGTACT AGT^ACT
SmlI 37 CTCAAG C^T[CT][GA]AG
Sse8387I 1624 CCTGCAGG CCTGCA^GG
StuI 720 AGGCCT AGG^CCT
StyI 196 CCTAGG C^C[AT][AT]GG
Van91I 127 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1150 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1294 GTAGAC GT^[AC][GT]AC
1640 GTCTAC
AccIII 1253 TCCGGA T^CCGGA
1663 TCCGGA
AlwNI 128 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
252 CAGACTCTG
BclI 936 TGATCA T^GATCA
1064 TGATCA
BsaWI 1253 TCCGGA [AT]^CCGG[AT]
1663 TCCGGA
BseAI 1253 TCCGGA T^CCGGA
1663 TCCGGA
BspEI 1253 TCCGGA T^CCGGA
1663 TCCGGA
BsrDI 1212 CATTGC GCAATG(2/0)
1404 CATTGC
BstYI 977 AGATCT [GA]^GATC[CT]
1593 GGATCT
DraII 1378 AGGACCT [GA]G^G[ACGT]CC[CT]
1564 GGGGCCT
EaeI 8 CGGCCG [CT]^GGCC[GA]
317 TGGCCA
EarI 734 GAAGAG CTCTTC(1/4)
1667 GAAGAG
EcoO109I 1378 AGGACCT [GA]G^G[ACGT]CC[CT]
1564 GGGGCCT
FbaI 936 TGATCA T^GATCA
1064 TGATCA
Ksp632I 734 GAAGAG CTCTTC(1/4)
1667 GAAGAG
MaeIII 652 GTGAC ^GT[ACGT]AC
1189 GTGAC
MflI 977 AGATCT [GA]^GATC[CT]
1593 GGATCT
MroI 1253 TCCGGA T^CCGGA
1663 TCCGGA
MspA1I 243 CAGCTG C[AC]G^C[GT]G
1757 CAGCTG
PleI 254 GACTC GAGTC(4/5)
436 GAGTC
PvuII 243 CAGCTG CAG^CTG
1757 CAGCTG
SfcI 1625 CTGCAG C^T[GA][CT]AG
1681 CTACAG
SphI 98 GCATGC GCATG^C
665 GCATGC
Tsp45I 652 GTGAC ^GT[GC]AC
1189 GTGAC
XhoII 977 AGATCT [GA]^GATC[CT]
1593 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I