(Circular) (Six-base) MAPSORT of: pFN21AB6696.seq Check: 6279 from: 1 to: 6091 pFN21AB6696 6091 bp With 182 enzymes: SgfI * November 19, 2009 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2766 2912 3220 4154 5538 5806 1632 Size: 1134 146 308 934 1384 268 1917 Fragments arranged by size: 1917 1384 1134 934 308 268 146 AfeI AGC'GCT Cuts at: 2203 2203 Size: 6091 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2646 4990 5967 1203 Size: 1443 2344 977 1327 Fragments arranged by size: 2344 1443 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 2379 4820 2379 Size: 2441 3650 AleI CACnn'nnGTG Cuts at: 1166 2166 1166 Size: 1000 5091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2184 1184 Size: 1000 5091 AlwNI CAG_nnn'CTG Cuts at: 2089 3377 3834 5406 2089 Size: 1288 457 1572 2774 Fragments arranged by size: 2774 1572 1288 457 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 3291 3400 3626 3778 5887 1493 Size: 1798 109 226 152 2109 1697 Fragments arranged by size: 2109 1798 1697 226 152 109 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3416 1870 Size: 73 1473 4545 Fragments arranged by size: 4545 1473 73 BamHI G'GATC_C Cuts at: 2772 3421 3788 2772 Size: 649 367 5075 Fragments arranged by size: 5075 649 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3412 4867 618 Size: 325 217 627 381 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2445 3410 1391 Size: 1054 965 4072 Fragments arranged by size: 4072 1054 965 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 2786 928 Size: 971 602 285 4233 Fragments arranged by size: 4233 971 602 285 BbvCI CC'TCA_GC Cuts at: 2115 2115 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 3983 5193 3260 Size: 723 1210 4158 Fragments arranged by size: 4158 1210 723 BclI T'GATC_A Cuts at: 1548 2391 1548 Size: 843 5248 BfrBI ATG'CAT Cuts at: 3206 3206 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2177 4702 136 Size: 107 122 71 631 605 505 2525 1525 Fragments arranged by size: 2525 1525 631 605 505 122 107 71 BglII A'GATC_T Cuts at: 2895 6086 2895 Size: 3191 2900 BlpI GC'TnA_GC Cuts at: 2016 3508 2016 Size: 1492 4599 Bme1580I G_kGCm'C Cuts at: 1790 2173 2369 4152 5308 1790 Size: 383 196 1783 1156 2573 Fragments arranged by size: 2573 1783 1156 383 196 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2887 3188 3822 Size: 416 53 83 399 24 1470 301 634 Cuts at: 3822 4780 442 Size: 958 1753 Fragments arranged by size: 1753 1470 958 634 416 399 301 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4751 1239 Size: 3512 2579 Bpu10I CC'TnA_GC Cuts at: 2115 2542 2115 Size: 427 5664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2233 2957 4216 5081 5379 2233 Size: 724 1259 865 298 2945 Fragments arranged by size: 2945 1259 865 724 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3303 4754 882 Size: 617 1804 1451 2219 Fragments arranged by size: 2219 1804 1451 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 2624 6085 2624 Size: 3461 2630 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3302 3332 1178 Size: 30 165 30 1899 30 3937 Fragments arranged by size: 3937 1899 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 1186 Size: 847 5244 BseYI C'CCAG_C Cuts at: 2260 2267 5294 5790 2260 Size: 7 3027 496 2561 Fragments arranged by size: 3027 2561 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2656 1163 Size: 573 920 4598 Fragments arranged by size: 4598 920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 2376 3541 4302 5330 1540 Size: 275 186 180 195 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 195 186 180 BsiHKAI G_wGCw'C Cuts at: 2148 2934 4152 4237 5308 2148 Size: 786 1218 85 1071 2931 Fragments arranged by size: 2931 1218 1071 786 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2173 2369 2445 2934 3410 4152 Size: 399 358 25 196 76 489 476 742 Cuts at: 4152 4237 5308 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 476 399 358 196 85 76 25 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2895 3446 844 Size: 2051 551 3489 Fragments arranged by size: 3489 2051 551 BsrBI CCG'CTC Cuts at: 1394 1823 3978 1394 Size: 429 2155 3507 Fragments arranged by size: 3507 2155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2694 3159 4586 4760 65 Size: 1183 1446 465 1427 174 1396 Fragments arranged by size: 1446 1427 1396 1183 465 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2375 4735 4922 1700 Size: 110 565 2360 187 2869 Fragments arranged by size: 2869 2360 565 187 110 BsrGI T'GTAC_A Cuts at: 96 2846 96 Size: 2750 3341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 3377 794 Size: 2583 3508 BstBI TT'CG_AA Cuts at: 3022 4895 3022 Size: 1873 4218 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstXI CCAn_nnnn'nTGG Cuts at: 2303 3334 2303 Size: 1031 5060 BstYI r'GATC_y Cuts at: 1972 2772 2895 3421 3788 4173 4190 5642 5728 Size: 800 123 526 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 800 526 385 367 346 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2190 3321 513 Size: 552 1125 1131 3283 Fragments arranged by size: 3283 1131 1125 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 2689 1554 Size: 1135 4956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2397 2594 Size: 54 1425 50 275 18 348 219 197 Cuts at: 2594 3324 3538 4427 6056 8 Size: 730 214 889 1629 43 Fragments arranged by size: 1629 1425 889 730 348 275 219 214 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2178 3538 1537 Size: 275 366 1360 4090 Fragments arranged by size: 4090 1360 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2450 2785 3265 4021 1353 Size: 1097 335 480 756 3423 Fragments arranged by size: 3423 1097 756 480 335 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2021 4678 5052 5198 387 Size: 1064 138 432 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 432 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 2912 3220 4154 4751 5538 5806 Size: 393 1134 146 308 934 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 1134 934 787 597 393 308 268 146 EcoRI G'AATT_C Cuts at: 3291 3400 3778 3291 Size: 109 378 5604 Fragments arranged by size: 5604 378 109 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2123 2205 5238 1678 Size: 445 82 3033 2531 Fragments arranged by size: 3033 2531 445 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2365 2397 2671 2703 2946 Size: 32 135 32 793 32 274 32 243 Cuts at: 2946 2978 3302 3334 4732 4764 4806 4838 1373 Size: 32 324 32 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 793 324 274 243 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3435 3681 669 Size: 988 281 349 1148 246 3079 Fragments arranged by size: 3079 1148 988 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 2224 3690 2224 Size: 1466 4625 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 2396 2891 3288 5205 5389 1305 Size: 1035 56 495 397 1917 184 2007 Fragments arranged by size: 2007 1917 1035 495 397 184 56 MscI TGG'CCA Cuts at: 10 64 1489 1832 2596 10 Size: 54 1425 343 764 3505 Fragments arranged by size: 3505 1425 764 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 2301 2408 2468 2489 2942 3035 Size: 648 1000 135 107 60 21 453 93 Cuts at: 3035 4049 4408 4567 518 Size: 1014 359 159 2042 Fragments arranged by size: 2042 1014 1000 648 453 359 159 135 107 93 60 21 MspA1I CmG'CkG Cuts at: 1606 2098 2166 3183 3507 4184 5332 5577 1606 Size: 492 68 1017 324 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 677 492 324 245 68 NcoI C'CATG_G Cuts at: 513 1065 2190 513 Size: 552 1125 4414 Fragments arranged by size: 4414 1125 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NsiI A_TGCA'T Cuts at: 3208 3208 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2650 2989 3449 4994 1132 Size: 97 1421 339 460 1545 2229 Fragments arranged by size: 2229 1545 1421 460 339 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 2646 4990 2646 Size: 2344 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 3183 3183 Size: 6091 RsrII CG'GwC_CG Cuts at: 2327 2327 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 2450 2450 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SexAI A'CCwGG_T Cuts at: 2870 2870 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 3439 4574 5255 5446 826 Size: 219 2394 1135 681 191 1471 Fragments arranged by size: 2394 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2248 2972 4195 5096 5358 820 Size: 197 926 305 724 1223 901 262 1553 Fragments arranged by size: 1553 1223 926 901 724 305 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2989 3449 1132 Size: 1857 460 3774 Fragments arranged by size: 3774 1857 460 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 2720 1517 Size: 1203 4888 StyI C'CwwG_G Cuts at: 513 1065 2190 2238 3108 3530 513 Size: 552 1125 48 870 422 3074 Fragments arranged by size: 3074 1125 870 552 422 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2012 2281 4118 4135 4288 4473 4918 Size: 1255 432 269 1837 17 153 185 445 Cuts at: 4918 325 Size: 1498 Fragments arranged by size: 1837 1498 1255 445 432 269 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2846 4337 6049 Size: 274 80 33 51 1443 869 1491 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 1712 1491 1443 869 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2407 2431 2874 3040 2407 Size: 24 443 166 5458 Fragments arranged by size: 5458 443 166 24 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 4220 4220 Size: 6091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SfiI SfoI SrfI SwaI