Restriction map for
pFN21AE1097
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BalI BbeI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII Eam1105I EcoRV
FbaI Hin1I KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1283 TCCGGA T^CCGGA
AcsI 413 GAATTC [GA]^AATT[CT]
Aor51HI 1244 AGCGCT AGC^GCT
ApoI 413 GAATTC [GA]^AATT[CT]
Asp700I 1339 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 794 TGCGCA TGC^GCA
BbrPI 1108 CACGTG CAC^GTG
BcgI 24 CGATGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1108 CACGTG [CT]AC^GT[GA]
BsaWI 1283 TCCGGA [AT]^CCGG[AT]
BseAI 1283 TCCGGA T^CCGGA
BsiEI 14 CGGCCG CG[GA][CT]^CG
BspEI 1283 TCCGGA T^CCGGA
BspMI 1232 ACCTGC ACCTGC(4/8)
BsrDI 1349 GCAATG GCAATG(2/0)
BssSI 1057 CACGAG CACGAG(-5/-1)
BstEII 1712 GGTCACC G^GT[ACGT]ACC
BstPI 1712 GGTCACC G^GT[ACGT]ACC
EagI 14 CGGCCG C^GGCCG
EclXI 14 CGGCCG C^GGCCG
Eco47III 1244 AGCGCT AGC^GCT
Eco52I 14 CGGCCG C^GGCCG
EcoO65I 1712 GGTCACC G^GT[ACGT]ACC
EcoRI 413 GAATTC G^AATTC
EcoT14I 1733 CCATGG C^C[AT][AT]GG
EcoT22I 973 ATGCAT ATGCA^T
FseI 106 GGCCGGCC GGCCGG^CC
FspI 794 TGCGCA TGC^GCA
HaeII 1244 AGCGCT [GA]GCGC^[CT]
HgaI 1043 GACGC GACGC(5/10)
HincII 541 GTTAAC GT[CT]^[GA]AC
HindII 541 GTTAAC GT[CT]^[GA]AC
HindIII 289 AAGCTT A^AGCTT
HpaI 541 GTTAAC GTT^AAC
MroI 1283 TCCGGA T^CCGGA
NcoI 1733 CCATGG C^CATGG
NsiI 973 ATGCAT ATGCA^T
PaeR7I 1544 CTCGAG C^TCGAG
PmaCI 1108 CACGTG CAC^GTG
PmlI 1108 CACGTG CAC^GTG
SacI 1183 GAGCTC GAGCT^C
StuI 1557 AGGCCT AGG^CCT
StyI 1733 CCATGG C^C[AT][AT]GG
TaqI 1545 TCGA T^CGA
TthHB8I 1545 TCGA T^CGA
XhoI 1544 CTCGAG C^TCGAG
XmnI 1339 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 658 GTGCAC G^TGCAC
1111 GTGCAC
ApaLI 658 GTGCAC G^TGCAC
1111 GTGCAC
AspI 267 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1707 GACAGGGTC
BsgI 1152 CTGCAC GTGCAG(16/14)
442 GTGCAG
BsrFI 16 GCCGGC [GA]^CCGG[CT]
107 GCCGGC
BstAPI 125 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1113 GCACAGCATGC
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
107 GCCGGC
DrdI 1273 GACCTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1478 GACCCCAGGGTC
EcoNI 564 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
624 CCTCACCCAGG
MseI 298 TTAA T^TAA
542 TTAA
NaeI 16 GCCGGC GCC^GGC
107 GCCGGC
NgoMIV 16 GCCGGC G^CCGGC
107 GCCGGC
PflFI 267 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1707 GACAGGGTC
PleI 241 GACTC GAGTC(4/5)
1030 GAGTC
SapI 558 GCTCTTC GCTCTTC(1/4)
780 GCTCTTC
SexAI 536 ACCAGGT A^CC[AT]GGT
896 ACCTGGT
SfaNI 31 GATGC GCATC(5/9)
866 GCATC
SmaI 1653 CCCGGG CCC^GGG
1779 CCCGGG
SmlI 1123 CTCAAG C^T[CT][GA]AG
1544 CTCGAG
SphI 887 GCATGC GCATG^C
1118 GCATGC
TfiI 168 GATTC G^A[AT]TC
1397 GATTC
Tru9I 298 TTAA T^TAA
542 TTAA
Tth111I 267 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1707 GACAGGGTC
XcmI 593 CCACAGCTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1761 CCAGGGTCCCCCTGG
XmaCI 1653 CCCGGG C^CCGGG
1779 CCCGGG
XmaI 1653 CCCGGG C^CCGGG
1779 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SfiI TseI Tsp45I Tsp509I
TspRI Van91I XhoII