Restriction map for
pFN21AE1096
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpmI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstAPI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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ApaI 526 GGGCCC GGGCC^C
AseI 1090 ATTAAT AT^TAAT
AsnI 1090 ATTAAT AT^TAAT
AvaI 1067 CTCGGG C^[CT]CG[GA]G
AviII 221 TGCGCA TGC^GCA
BanII 526 GGGCCC G[GA]GC[CT]^C
BbsI 673 GTCTTC GAAGAC(2/6)
BlpI 408 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 408 GCTGAGC GC^T[ACGT]AGC
BpuAI 673 GTCTTC GAAGAC(2/6)
BsaAI 838 CACGTA [CT]AC^GT[GA]
BsgI 375 CTGCAC GTGCAG(16/14)
BsoBI 1067 CTCGGG C^[CT]CG[GA]G
Bsp1407I 965 TGTACA T^GTACA
BsrFI 317 ACCGGC [GA]^CCGG[CT]
BsrGI 965 TGTACA T^GTACA
BstBI 1018 TTCGAA TT^CGAA
BstEII 462 GGTCACC G^GT[ACGT]ACC
BstPI 462 GGTCACC G^GT[ACGT]ACC
CelII 408 GCTGAGC GC^T[ACGT]AGC
Cfr10I 317 ACCGGC [GA]^CCGG[CT]
EaeI 47 TGGCCG [CT]^GGCC[GA]
EcoNI 752 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 462 GGTCACC G^GT[ACGT]ACC
FspI 221 TGCGCA TGC^GCA
MaeII 839 ACGT A^CGT
MseI 1091 TTAA T^TAA
MspA1I 121 CCGCTG C[AC]G^C[GT]G
NspI 284 GCATGC [GA]CATG^[CT]
NspV 1018 TTCGAA TT^CGAA
PleI 82 GACTC GAGTC(4/5)
PshBI 1090 ATTAAT AT^TAAT
ScaI 905 AGTACT AGT^ACT
SfuI 1018 TTCGAA TT^CGAA
SphI 284 GCATGC GCATG^C
SspBI 965 TGTACA T^GTACA
Tru9I 1091 TTAA T^TAA
Tsp509I 359 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AcyI 12 GACGCC G[GA]^CG[CT]C
133 GGCGTC
Aor51HI 405 AGCGCT AGC^GCT
419 AGCGCT
AspHI 391 GTGCTC G[AT]GC[AT]^C
553 GAGCAC
BanI 565 GGCACC G^G[CT][GA]CC
748 GGTGCC
BfaI 541 CTAG C^TAG
1085 CTAG
BsaHI 12 GACGCC G[GA]^CG[CT]C
133 GGCGTC
BseRI 340 CTCCTC GAGGAG(10/8)
723 GAGGAG
BsiHKAI 391 GTGCTC G[AT]GC[AT]^C
553 GAGCAC
BsmAI 680 GTCTC GTCTC(1/5)
698 GTCTC
BsmFI 578 GGGAC GGGAC(10/14)
761 GGGAC
BspMI 145 ACCTGC ACCTGC(4/8)
182 ACCTGC
BssSI 556 CACGAG CACGAG(-5/-1)
916 CTCGTG
BstF5I 816 CATCC GGATG(2/0)
63 GGATG
DpnII 265 GATC ^GATC
897 GATC
DsaI 386 CCACGG C^C[GA][CT]GG
506 CCGTGG
Eco47III 405 AGCGCT AGC^GCT
419 AGCGCT
FokI 816 CATCC GGATG(9/13)
63 GGATG
Hin1I 12 GACGCC G[GA]^CG[CT]C
133 GGCGTC
MaeI 541 CTAG C^TAG
1085 CTAG
MaeIII 136 GTCAC ^GT[ACGT]AC
463 GTCAC
MboI 265 GATC ^GATC
897 GATC
NdeII 265 GATC ^GATC
897 GATC
PstI 103 CTGCAG CTGCA^G
516 CTGCAG
SapI 22 GAAGAGC GCTCTTC(1/4)
108 GCTCTTC
Sau3AI 265 GATC ^GATC
897 GATC
TaqI 416 TCGA T^CGA
1019 TCGA
TfiI 1016 GATTC G^A[AT]TC
1081 GAATC
Tsp45I 136 GTCAC ^GT[GC]AC
463 GTCAC
TthHB8I 416 TCGA T^CGA
1019 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI EarI EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI SfcI StyI TseI TspRI