(Circular) (Six-base) MAPSORT of: pFN21AE1096.seq Check: 8263 from: 1 to: 5803 pFN21AE1096 5803 bp With 182 enzymes: SgfI * November 19, 2009 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2159 2159 Size: 5803 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3866 5250 5518 1632 Size: 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 268 AfeI AGC'GCT Cuts at: 2412 2426 2412 Size: 14 5789 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 2153 1166 Size: 987 4816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5803 AlwNI CAG_nnn'CTG Cuts at: 3546 5118 3546 Size: 1572 4231 ApaI G_GGCC'C Cuts at: 2535 2535 Size: 5803 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 3096 4357 160 Size: 872 2064 1261 1606 Fragments arranged by size: 2064 1606 1261 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 3072 3128 1870 Size: 73 1129 56 4545 Fragments arranged by size: 4545 1129 73 56 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2570 2753 3124 4579 618 Size: 325 217 627 783 183 371 1455 1842 Fragments arranged by size: 1842 1455 783 627 371 325 217 183 BanII G_rGCy'C Cuts at: 1391 2535 3122 1391 Size: 1144 587 4072 Fragments arranged by size: 4072 1144 587 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2671 928 Size: 971 772 4060 Fragments arranged by size: 4060 971 772 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 2414 3220 2414 Size: 806 4997 Bme1580I G_kGCm'C Cuts at: 1790 2535 3864 5020 1790 Size: 745 1329 1156 2573 Fragments arranged by size: 2573 1329 1156 745 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5803 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2074 3534 4492 Size: 416 53 83 399 24 657 1460 958 Cuts at: 4492 442 Size: 1753 Fragments arranged by size: 1753 1460 958 657 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2899 2899 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4463 1239 Size: 3224 2579 Bpu10I CC'TnA_GC Cuts at: 2762 2762 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 3594 493 Size: 711 1641 749 2702 Fragments arranged by size: 2702 1641 749 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2139 3610 3992 Size: 53 83 186 936 485 121 1471 382 Cuts at: 3992 4627 275 Size: 635 1451 Fragments arranged by size: 1471 1451 936 635 485 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5578 Fragments arranged by size: 5578 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2743 1186 Size: 1150 407 4246 Fragments arranged by size: 4246 1150 407 BseYI C'CCAG_C Cuts at: 2021 2092 5006 5502 2021 Size: 71 2914 496 2322 Fragments arranged by size: 2914 2322 496 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2365 1163 Size: 573 629 4601 Fragments arranged by size: 4601 629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2562 3864 3949 5020 2400 Size: 162 1302 85 1071 3183 Fragments arranged by size: 3183 1302 1071 162 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2400 2535 2562 3122 3864 3949 5020 Size: 399 610 135 27 560 742 85 1071 Cuts at: 5020 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 610 560 399 135 85 27 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 2196 3158 844 Size: 1315 37 962 3489 Fragments arranged by size: 3489 1315 962 37 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2322 4447 4634 1700 Size: 110 512 2125 187 2869 Fragments arranged by size: 2869 2125 512 187 110 BsrGI T'GTAC_A Cuts at: 96 2970 96 Size: 2874 2929 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 2561 2921 3863 4875 2561 Size: 360 942 1012 3489 Fragments arranged by size: 3489 1012 942 360 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5803 BstBI TT'CG_AA Cuts at: 3024 4607 3024 Size: 1583 4220 BstEII G'GTnAC_C Cuts at: 1184 2467 1184 Size: 1283 4520 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2203 2464 2155 Size: 48 261 5494 Fragments arranged by size: 5494 261 48 BstYI r'GATC_y Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798 Size: 1161 367 385 17 1452 86 12 346 Cuts at: 5798 1972 Size: 1977 Fragments arranged by size: 1977 1452 1161 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2391 2511 513 Size: 552 1326 120 3805 Fragments arranged by size: 3805 1326 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2161 2754 487 Size: 683 56 935 593 3536 Fragments arranged by size: 3536 935 683 593 56 BtsI GCAGTG_nn' Cuts at: 3009 3319 4113 4133 3009 Size: 310 794 20 4679 Fragments arranged by size: 4679 794 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2593 2628 529 Size: 35 2029 35 3704 Fragments arranged by size: 3704 2029 35 35 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 3250 4139 Size: 54 1425 50 275 18 222 1198 889 Cuts at: 4139 5768 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1198 889 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2120 2168 2363 2942 3733 1353 Size: 669 98 48 195 579 791 3423 Fragments arranged by size: 3423 791 669 579 195 98 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2740 4390 4764 4910 387 Size: 1064 138 1151 1650 374 146 1280 Fragments arranged by size: 1650 1280 1151 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3866 4463 5250 5518 1239 Size: 393 2234 597 787 268 1524 Fragments arranged by size: 2234 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2761 2761 Size: 5803 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FspI TGC'GCA Cuts at: 2228 4309 2228 Size: 2081 3722 HaeII r_GCGC'y Cuts at: 1678 2414 2428 2529 4950 1678 Size: 736 14 101 2421 2531 Fragments arranged by size: 2531 2421 736 101 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373 Size: 32 135 32 2872 32 42 32 2626 Fragments arranged by size: 2872 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2153 2201 2540 2639 3761 4120 4279 Size: 648 987 48 339 99 1122 359 159 Cuts at: 4279 518 Size: 2042 Fragments arranged by size: 2042 1122 987 648 359 339 159 99 48 MspA1I CmG'CkG Cuts at: 1606 2128 3219 3896 5044 5289 1606 Size: 522 1091 677 1148 245 2120 Fragments arranged by size: 2120 1148 1091 677 522 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2293 3161 4706 1132 Size: 97 1064 868 1545 2229 Fragments arranged by size: 2229 1545 1064 868 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2021 2053 3872 3904 5385 5417 2021 Size: 32 1819 32 1481 32 2407 Fragments arranged by size: 2407 1819 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2531 2531 Size: 5803 PstI C_TGCA'G Cuts at: 830 2112 2525 3155 830 Size: 1282 413 630 3478 Fragments arranged by size: 3478 1282 630 413 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SapI GCTCTTCn'nnn_ Cuts at: 2022 2120 2022 Size: 98 5705 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 2912 4051 5763 2912 Size: 1139 1712 2952 Fragments arranged by size: 2952 1712 1139 SfcI C'TryA_G Cuts at: 826 1045 2108 2521 2698 2802 3151 4286 4967 Size: 219 1063 413 177 104 349 1135 681 Cuts at: 4967 5158 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1063 681 413 349 219 191 177 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 2293 3161 1132 Size: 1161 868 3774 Fragments arranged by size: 3774 1161 868 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 2457 2472 2579 2886 3242 513 Size: 552 1392 15 107 307 356 3074 Fragments arranged by size: 3074 1392 552 356 307 107 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2638 2741 3830 3847 4000 4185 4630 Size: 1255 1058 103 1089 17 153 185 445 Cuts at: 4630 325 Size: 1498 Fragments arranged by size: 1498 1255 1089 1058 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2910 2970 4049 Size: 274 80 33 51 1443 933 60 1079 Cuts at: 4049 5761 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1079 933 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5763 5795 5763 Size: 32 5771 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI