##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AluI           85  AGCT                 AG^CT
AlwNI          12  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI            2  TGGCCA               TGG^CCA
BbsI          146  GAAGAC               GAAGAC(2/6)
BpuAI         146  GAAGAC               GAAGAC(2/6)
BsaJI         124  CCCAGG               C^C[ACGT][ACGT]GG
BseRI          99  GAGGAG               GAGGAG(10/8)
BsmAI          54  GAGAC                GTCTC(1/5)
DrdI           56  GACAAAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI            2  TGGCCA               [CT]^GGCC[GA]
HaeIII          3  GGCC                 GG^CC
HapII          33  CCGG                 C^CGG
HpaII          33  CCGG                 C^CGG
MboII         146  GAAGA                GAAGA(8/7)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MspI           33  CCGG                 C^CGG
NlaIII        108  CATG                 CATG^
SfaNI          14  GATGC                GCATC(5/9)


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI            9  GTAC                 GT^AC
              129  GTAC                
BssKI           5  CCAGG                ^CC[ACGT]GG
              125  CCAGG               
BstNI           5  CCAGG                CC^[AT]GG
              125  CCAGG               
EcoRII          5  CCAGG                ^CC[AT]GG
              125  CCAGG               
MvaI            5  CCAGG                CC^[AT]GG
              125  CCAGG               
MwoI           17  GCTGTCGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               23  GCAGCCAGAGC         
RsaI            9  GTAC                 GT^AC
              129  GTAC                
ScrFI           5  CCAGG                CC^[ACGT]GG
              125  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AciI        BbvI        Fnu4HI      ItaI        MnlI        
TseI