(Circular) (Six-base) MAPSORT of: pFN21AE6133.seq Check: 9754 from: 1 to: 4858 pFN21AE6133 4858 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2668 278 Size: 53 83 186 936 1132 2468 Fragments arranged by size: 2468 1132 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2201 1937 Size: 264 4594 Acc65I G'GTAC_C Cuts at: 2179 2179 Size: 4858 AclI AA'CG_TT Cuts at: 1563 2985 3358 1563 Size: 1422 373 3063 Fragments arranged by size: 3063 1422 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2921 4305 4573 1632 Size: 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4661 AflIII A'CryG_T Cuts at: 1203 2155 3757 4734 1203 Size: 952 1602 977 1327 Fragments arranged by size: 1602 1327 977 952 AgeI A'CCGG_T Cuts at: 3689 3689 Size: 4858 AhdI GACnn_n'nnGTC Cuts at: 3587 3587 Size: 4858 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4858 AlwNI CAG_nnn'CTG Cuts at: 2022 2601 4173 2022 Size: 579 1572 2707 Fragments arranged by size: 2707 1572 579 ApaLI G'TGCA_C Cuts at: 2915 4071 2915 Size: 1156 3702 ApoI r'AATT_y Cuts at: 1493 2167 2393 2545 4654 1493 Size: 674 226 152 2109 1697 Fragments arranged by size: 2109 1697 674 226 152 AseI AT'TA_AT Cuts at: 160 1032 3412 160 Size: 872 2380 1606 Fragments arranged by size: 2380 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4858 AvaI C'yCGr_G Cuts at: 1870 1943 2183 1870 Size: 73 240 4545 Fragments arranged by size: 4545 240 73 BamHI G'GATC_C Cuts at: 2188 2555 2188 Size: 367 4491 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 3634 618 Size: 325 217 627 392 1455 1842 Fragments arranged by size: 1842 1455 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2177 1391 Size: 786 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 928 Size: 971 259 3628 Fragments arranged by size: 3628 971 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3049 3083 1903 Size: 34 1112 34 3678 Fragments arranged by size: 3678 1112 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2750 3960 2750 Size: 1210 3648 BclI T'GATC_A Cuts at: 1548 1548 Size: 4858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3469 136 Size: 107 122 71 631 605 1797 1525 Fragments arranged by size: 1797 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4853 4853 Size: 4858 BlpI GC'TnA_GC Cuts at: 2275 2275 Size: 4858 Bme1580I G_kGCm'C Cuts at: 1790 2919 4075 1790 Size: 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4858 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2589 3547 442 Size: 416 53 83 399 24 1172 958 1753 Fragments arranged by size: 1753 1172 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4858 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3518 1239 Size: 2279 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2983 3848 4146 2983 Size: 865 298 3695 Fragments arranged by size: 3695 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3521 882 Size: 617 2022 2219 Fragments arranged by size: 2219 2022 617 BsaAI yAC'GTr Cuts at: 493 1204 2649 493 Size: 711 1445 2702 Fragments arranged by size: 2702 1445 711 BsaBI GATnn'nnATC Cuts at: 4852 4852 Size: 4858 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2665 3047 3682 275 Size: 53 83 186 936 1132 382 635 1451 Fragments arranged by size: 1451 1132 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 3689 3963 4110 4521 3291 Size: 398 274 147 411 3628 Fragments arranged by size: 3628 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4633 Fragments arranged by size: 4633 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 1186 Size: 933 3925 BseYI C'CCAG_C Cuts at: 4061 4557 4061 Size: 496 4362 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2308 3069 4097 1540 Size: 275 186 307 761 1028 2301 Fragments arranged by size: 2301 1028 761 307 275 186 BsiHKAI G_wGCw'C Cuts at: 2919 3004 4075 2919 Size: 85 1071 3702 Fragments arranged by size: 3702 1071 85 BsmI GAATG_Cn' Cuts at: 2369 2462 2369 Size: 93 4765 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2919 3004 4075 1391 Size: 399 387 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 85 BspEI T'CCGG_A Cuts at: 4521 4521 Size: 4858 BspHI T'CATG_A Cuts at: 2747 4477 2747 Size: 1730 3128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2213 844 Size: 1369 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2745 1394 Size: 429 922 3507 Fragments arranged by size: 3507 922 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3353 3527 65 Size: 1183 2105 174 1396 Fragments arranged by size: 2105 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3502 3689 1700 Size: 110 1692 187 2869 Fragments arranged by size: 2869 1692 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4858 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4858 BssSI C'ACGA_G Cuts at: 2918 3930 2918 Size: 1012 3846 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4858 BstBI TT'CG_AA Cuts at: 3662 3662 Size: 4858 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4858 BstYI r'GATC_y Cuts at: 1972 2188 2555 2940 2957 4409 4495 4507 4853 Size: 216 367 385 17 1452 86 12 346 Cuts at: 4853 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3367 Fragments arranged by size: 3367 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4119 Fragments arranged by size: 4119 683 56 BtsI GCAGTG_nn' Cuts at: 2374 3168 3188 2374 Size: 794 20 4044 Fragments arranged by size: 4044 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4858 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4823 DraI TTT'AAA Cuts at: 2162 2162 Size: 4858 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2067 3865 809 Size: 507 751 1798 1802 Fragments arranged by size: 1802 1798 751 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2305 3194 Size: 54 1425 50 275 18 177 298 889 Cuts at: 3194 4823 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 298 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2305 1537 Size: 275 493 4090 Fragments arranged by size: 4090 493 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2788 1353 Size: 1435 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 3445 3819 3965 387 Size: 1064 138 512 1344 374 146 1280 Fragments arranged by size: 1344 1280 1064 512 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2921 3518 4305 4573 1239 Size: 393 1289 597 787 268 1524 Fragments arranged by size: 1524 1289 787 597 393 268 EcoRI G'AATT_C Cuts at: 2167 2545 2167 Size: 378 4480 FspI TGC'GCA Cuts at: 3364 3364 Size: 4858 HaeII r_GCGC'y Cuts at: 1678 4005 1678 Size: 2327 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3499 3531 3573 3605 1373 Size: 32 135 32 1927 32 42 32 2626 Fragments arranged by size: 2626 1927 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2202 2448 669 Size: 988 281 264 246 3079 Fragments arranged by size: 3079 988 281 264 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4858 HpaI GTT'AAC Cuts at: 2448 2448 Size: 4858 KpnI G_GTAC'C Cuts at: 2183 2183 Size: 4858 MfeI C'AATT_G Cuts at: 2457 2457 Size: 4858 MluI A'CGCG_T Cuts at: 4734 4734 Size: 4858 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3972 4156 1305 Size: 2667 184 2007 Fragments arranged by size: 2667 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 2859 Fragments arranged by size: 2859 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2816 3175 3334 518 Size: 648 1650 359 159 2042 Fragments arranged by size: 2042 1650 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2274 2951 4099 4344 1606 Size: 668 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3367 Fragments arranged by size: 3367 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4858 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4858 NotI GC'GGCC_GC Cuts at: 2305 2305 Size: 4858 NspI r_CATG'y Cuts at: 1132 1229 2216 3761 1132 Size: 97 987 1545 2229 Fragments arranged by size: 2229 1545 987 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4858 PciI A'CATG_T Cuts at: 3757 3757 Size: 4858 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4858 PmeI GTTT'AAAC Cuts at: 2162 2162 Size: 4858 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2927 2959 4440 4472 2927 Size: 32 1481 32 3313 Fragments arranged by size: 3313 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4858 PsiI TTA'TAA Cuts at: 2428 2428 Size: 4858 PstI C_TGCA'G Cuts at: 830 2210 830 Size: 1380 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4858 SalI G'TCGA_C Cuts at: 1936 2200 1936 Size: 264 4594 SbfI CC_TGCA'GG Cuts at: 2210 2210 Size: 4858 ScaI AGT'ACT Cuts at: 3106 4818 3106 Size: 1712 3146 SfcI C'TryA_G Cuts at: 826 1045 2206 3341 4022 4213 826 Size: 219 1161 1135 681 191 1471 Fragments arranged by size: 1471 1161 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4858 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4858 SmaI CCC'GGG Cuts at: 1872 2185 1872 Size: 313 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2962 3863 4125 820 Size: 197 926 1019 901 262 1553 Fragments arranged by size: 1553 1019 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2649 493 Size: 2156 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4858 SphI G_CATG'C Cuts at: 1132 2216 1132 Size: 1084 3774 SspI AAT'ATT Cuts at: 5 52 2782 5 Size: 47 2730 2081 Fragments arranged by size: 2730 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4858 StyI C'CwwG_G Cuts at: 513 1065 2004 2297 513 Size: 552 939 293 3074 Fragments arranged by size: 3074 939 552 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 2885 2902 3055 3240 3685 325 Size: 1255 575 730 17 153 185 445 1498 Fragments arranged by size: 1498 1255 730 575 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3104 4816 96 Size: 274 80 33 51 1443 1127 1712 138 Fragments arranged by size: 1712 1443 1127 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4818 4850 4818 Size: 32 4826 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4858 XbaI T'CTAG_A Cuts at: 2194 2194 Size: 4858 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4858 XmaI C'CCGG_G Cuts at: 1870 2183 1870 Size: 313 4545 XmnI GAAnn'nnTTC Cuts at: 2987 2987 Size: 4858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2666 276 Size: 53 83 186 936 1132 2468 Fragments arranged by size: 2468 1132 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI