################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HphI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MscI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 17 TCCGGA T^CCGGA AcsI 75 GAATTT [GA]^AATT[CT] AfaI 129 GTAC GT^AC AluI 114 AGCT AG^CT AlwI 136 GGATC GGATC(4/5) ApaI 63 GGGCCC GGGCC^C ApoI 75 GAATTT [GA]^AATT[CT] AvaII 39 GGTCC G^G[AT]CC BanII 63 GGGCCC G[GA]GC[CT]^C BfaI 112 CTAG C^TAG BmyI 63 GGGCCC G[AGT]GC[ACT]^C BsaWI 17 TCCGGA [AT]^CCGG[AT] BseAI 17 TCCGGA T^CCGGA BseRI 58 GAGGAG GAGGAG(10/8) BsiYI 51 CCACTATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 51 CCACTATGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 63 GGGCCC G[AGT]GC[ACT]^C BspEI 17 TCCGGA T^CCGGA BssKI 67 CCTGG ^CC[ACGT]GG BstF5I 15 CATCC GGATG(2/0) BstNI 67 CCTGG CC^[AT]GG BstYI 136 GGATCT [GA]^GATC[CT] DdeI 116 CTAAG C^T[ACGT]AG DpnII 137 GATC ^GATC EcoRII 67 CCTGG ^CC[AT]GG FokI 15 CATCC GGATG(9/13) HapII 18 CCGG C^CGG HpaII 18 CCGG C^CGG MaeI 112 CTAG C^TAG MaeIII 108 GTTAC ^GT[ACGT]AC MboI 137 GATC ^GATC MboII 72 GAAGA GAAGA(8/7) MflI 136 GGATCT [GA]^GATC[CT] MroI 17 TCCGGA T^CCGGA MseI 182 TTAA T^TAA MspI 18 CCGG C^CGG MvaI 67 CCTGG CC^[AT]GG NdeII 137 GATC ^GATC RsaI 129 GTAC GT^AC Sau3AI 137 GATC ^GATC ScrFI 67 CCTGG CC^[ACGT]GG SfaNI 14 GCATC GCATC(5/9) Tru9I 182 TTAA T^TAA Tsp509I 76 AATT ^AATT TspRI 89 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 136 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BsaJI 66 CCCTGG C^C[ACGT][ACGT]GG 67 CCTGGG DraII 62 AGGGCCC [GA]G^G[ACGT]CC[CT] 63 GGGCCCT EcoO109I 62 AGGGCCC [GA]G^G[ACGT]CC[CT] 63 GGGCCCT HaeIII 7 GGCC GG^CC 64 GGCC NlaIV 47 GGAGCC GG[ACGT]^[ACGT]CC 63 GGGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### Cfr13I MnlI Sau96I