Restriction map for
pFN21AB6181
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FauI FseI FspI HgaI
KpnI KspI MflI MluI MluNI
MroI MscI NaeI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacII SapI SbfI SfcI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI Tsp45I
Van91I XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 1484 GACGTC GACGT^C
AccI 1807 GTCGAC GT^[AC][GT]AC
AlwI 1365 GATCC GGATC(4/5)
AlwNI 205 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 684 GGGCCC GGGCC^C
AseI 1179 ATTAAT AT^TAAT
AsnI 1179 ATTAAT AT^TAAT
AspHI 677 GAGCTC G[AT]GC[AT]^C
AspI 1639 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 86 GGCGCC GGCGC^C
BclI 1326 TGATCA T^GATCA
BpmI 1289 CTGGAG CTGGAG(16/14)
BsaBI 1198 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 677 GAGCTC G[AT]GC[AT]^C
BspHI 1896 TCATGA T^CATGA
BspMI 1907 ACCTGC ACCTGC(4/8)
BsrFI 1153 GCCGGT [GA]^CCGG[CT]
BssKI 1282 CCTGG ^CC[ACGT]GG
BstBI 740 TTCGAA TT^CGAA
BstEII 985 GGTTACC G^GT[ACGT]ACC
BstNI 1282 CCTGG CC^[AT]GG
BstPI 985 GGTTACC G^GT[ACGT]ACC
Cfr10I 1153 GCCGGT [GA]^CCGG[CT]
DraI 1200 TTTAAA TTT^AAA
DraII 683 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 1151 TGGCCG [CT]^GGCC[GA]
EarI 1858 CTCTTC CTCTTC(1/4)
EcoO109I 683 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 985 GGTTACC G^GT[ACGT]ACC
EcoRI 1936 GAATTC G^AATTC
EcoRII 1282 CCTGG ^CC[AT]GG
EcoT14I 1545 CCTTGG C^C[AT][AT]GG
FbaI 1326 TGATCA T^GATCA
HaeII 86 GGCGCC [GA]GCGC^[CT]
HapII 1154 CCGG C^CGG
HindIII 503 AAGCTT A^AGCTT
HpaI 660 GTTAAC GTT^AAC
HpaII 1154 CCGG C^CGG
HphI 1459 GGTGA GGTGA(8/7)
KasI 86 GGCGCC G^GCGCC
Ksp632I 1858 CTCTTC CTCTTC(1/4)
MamI 1198 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 671 CAATTG C^AATTG
MslI 1912 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 559 CAGCTG C[AC]G^C[GT]G
MspI 1154 CCGG C^CGG
MunI 671 CAATTG C^AATTG
MvaI 1282 CCTGG CC^[AT]GG
NarI 86 GGCGCC GG^CGCC
NdeI 1912 CATATG CA^TATG
NspV 740 TTCGAA TT^CGAA
PflFI 1639 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1179 ATTAAT AT^TAAT
PvuII 559 CAGCTG CAG^CTG
RcaI 1896 TCATGA T^CATGA
SacI 677 GAGCTC GAGCT^C
SalI 1807 GTCGAC G^TCGAC
ScrFI 1282 CCTGG CC^[ACGT]GG
SexAI 1281 ACCTGGT A^CC[AT]GGT
SfaNI 1850 GCATC GCATC(5/9)
SfoI 86 GGCGCC GGC^GCC
SfuI 740 TTCGAA TT^CGAA
StyI 1545 CCTTGG C^C[AT][AT]GG
SwaI 1199 ATTTAAAT ATTT^AAAT
Tth111I 1639 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1059 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 86 GGCGCC G[GA]^CG[CT]C
1484 GACGTC
Asp700I 357 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1433 GAAAGAGTTC
AvaII 222 GGTCC G^G[AT]CC
1417 GGTCC
BanI 49 GGTGCC G^G[CT][GA]CC
86 GGCGCC
BanII 677 GAGCTC G[GA]GC[CT]^C
684 GGGCCC
BbsI 743 GAAGAC GAAGAC(2/6)
883 GTCTTC
BmyI 677 GAGCTC G[AGT]GC[ACT]^C
684 GGGCCC
BpuAI 743 GAAGAC GAAGAC(2/6)
883 GTCTTC
BsaHI 86 GGCGCC G[GA]^CG[CT]C
1484 GACGTC
BsaJI 96 CCTCGG C^C[ACGT][ACGT]GG
1545 CCTTGG
BseRI 36 CTCCTC GAGGAG(10/8)
490 GAGGAG
BsgI 103 CTGCAC GTGCAG(16/14)
1796 GTGCAG
BsmI 1085 GAATGC GAATGC(1/-1)
1786 GAATGC
Bsp1286I 677 GAGCTC G[AGT]GC[ACT]^C
684 GGGCCC
EcoNI 483 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1498 CCTGAAAGAGG
Hin1I 86 GGCGCC G[GA]^CG[CT]C
1484 GACGTC
HincII 660 GTTAAC GT[CT]^[GA]AC
1807 GTCGAC
HindII 660 GTTAAC GT[CT]^[GA]AC
1807 GTCGAC
XmnI 357 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1433 GAAAGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI BbvI BfaI BsaI BsiYI
BslI BsmAI BsmFI BsrI BstF5I
BstUI Cac8I CfoI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScaI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I