Restriction map for
pFN21AB9655
(Linear) (Six-base) MAPSORT of: KIBB9655.seq Check: 1117 from: 1 to: 2046
KIBB9655.orf
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AatII G_ACGT'C
Cuts at: 0 233 2046
Size: 233 1813
AfeI AGC'GCT
Cuts at: 0 1777 2046
Size: 1777 269
AleI CACnn'nnGTG
Cuts at: 0 838 1936 2046
Size: 838 1098 110
Fragments arranged by size:
1098 838 110
ApaI G_GGCC'C
Cuts at: 0 1269 1357 2046
Size: 1269 88 689
Fragments arranged by size:
1269 689 88
ApoI r'AATT_y
Cuts at: 0 46 646 2046
Size: 46 600 1400
Fragments arranged by size:
1400 600 46
AscI GG'CGCG_CC
Cuts at: 0 1430 2046
Size: 1430 616
AvaI C'yCGr_G
Cuts at: 0 1261 1338 1357 1425 1884 2046
Size: 1261 77 19 68 459 162
Fragments arranged by size:
1261 459 162 77 68 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 601 634 2046
Size: 601 33 1412
Fragments arranged by size:
1412 601 33
BamHI G'GATC_C
Cuts at: 0 85 777 2046
Size: 85 692 1269
Fragments arranged by size:
1269 692 85
BanI G'GyrC_C
Cuts at: 0 597 656 1636 1663 1729 1795 2046
Size: 597 59 980 27 66 66 251
Fragments arranged by size:
980 597 251 66 66 59 27
BanII G_rGCy'C
Cuts at: 0 561 891 1269 1357 1678 2046
Size: 561 330 378 88 321 368
Fragments arranged by size:
561 378 368 330 321 88
BbeI G_GCGC'C
Cuts at: 0 1640 1733 1799 2046
Size: 1640 93 66 247
Fragments arranged by size:
1640 247 93 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 314 832 866 2046
Size: 280 34 518 34 1180
Fragments arranged by size:
1180 518 280 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 496 2046
Size: 496 1550
BclI T'GATC_A
Cuts at: 0 671 2046
Size: 671 1375
BfrBI ATG'CAT
Cuts at: 0 864 2046
Size: 864 1182
BglI GCCn_nnn'nGGC
Cuts at: 0 639 1058 1607 1692 2046
Size: 639 419 549 85 354
Fragments arranged by size:
639 549 419 354 85
Bme1580I G_kGCm'C
Cuts at: 0 602 1262 1269 1357 2046
Size: 602 660 7 88 689
Fragments arranged by size:
689 660 602 88 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 893 2046
Size: 353 540 1153
Fragments arranged by size:
1153 540 353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1869 2046
Size: 1869 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 157 810 1860 2046
Size: 28 129 653 1050 186
Fragments arranged by size:
1050 653 186 129 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1945 2046
Size: 1945 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 273 733 2046
Size: 273 460 1313
Fragments arranged by size:
1313 460 273
BsaBI GATnn'nnATC
Cuts at: 0 673 782 2046
Size: 673 109 1264
Fragments arranged by size:
1264 673 109
BsaHI Gr'CG_yC
Cuts at: 0 230 1459 1637 1730 1796 1808 1849 2046
Size: 230 1229 178 93 66 12 41 197
Fragments arranged by size:
1229 230 197 178 93 66 41 12
BsaWI w'CCGG_w
Cuts at: 0 867 2046
Size: 867 1179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 589 2046
Size: 529 30 30 1457
Fragments arranged by size:
1457 529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 480 567 1765 1845 2046
Size: 307 173 87 1198 80 201
Fragments arranged by size:
1198 307 201 173 87 80
BseYI C'CCAG_C
Cuts at: 0 584 1631 1979 2046
Size: 584 1047 348 67
Fragments arranged by size:
1047 584 348 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1575 2046
Size: 1087 488 471
Fragments arranged by size:
1087 488 471
BsiEI CG_ry'CG
Cuts at: 0 515 1062 1712 1928 2046
Size: 515 547 650 216 118
Fragments arranged by size:
650 547 515 216 118
BsiHKAI G_wGCw'C
Cuts at: 0 14 561 2046
Size: 14 547 1485
Fragments arranged by size:
1485 547 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1840 2046
Size: 1840 206
Bsp1286I G_dGCh'C
Cuts at: 0 14 561 602 891 1262 1269 1357 1678
Size: 14 547 41 289 371 7 88 321
Cuts at: 1678 2046
Size: 368
Fragments arranged by size:
547 371 368 321 289 88 41 14
7
BspEI T'CCGG_A
Cuts at: 0 867 2046
Size: 867 1179
BsrBI CCG'CTC
Cuts at: 0 1605 1959 2046
Size: 1605 354 87
Fragments arranged by size:
1605 354 87
BsrFI r'CCGG_y
Cuts at: 0 393 514 653 1198 1282 1461 1472 1726
Size: 393 121 139 545 84 179 11 254
Cuts at: 1726 1923 2046
Size: 197 123
Fragments arranged by size:
545 393 254 197 179 139 123 121
84 11
BssHII G'CGCG_C
Cuts at: 0 1430 1717 2046
Size: 1430 287 329
Fragments arranged by size:
1430 329 287
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1257 2046
Size: 1257 789
BstYI r'GATC_y
Cuts at: 0 85 777 1916 2046
Size: 85 692 1139 130
Fragments arranged by size:
1139 692 130 85
BtgI C'CryG_G
Cuts at: 0 509 737 1277 1508 1706 2046
Size: 509 228 540 231 198 340
Fragments arranged by size:
540 509 340 231 228 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1083 2046
Size: 1083 963
BtsI GCAGTG_nn'
Cuts at: 0 12 350 2046
Size: 12 338 1696
Fragments arranged by size:
1696 338 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 183 2046
Size: 183 1863
EaeI y'GGCC_r
Cuts at: 0 347 381 512 540 1059 1280 1463 1505
Size: 347 34 131 28 519 221 183 42
Cuts at: 1505 1529 1596 1709 1724 1766 1925 2046
Size: 24 67 113 15 42 159 121
Fragments arranged by size:
519 347 221 183 159 131 121 113
67 42 42 34 28 24 15
EagI C'GGCC_G
Cuts at: 0 512 1059 1709 1925 2046
Size: 512 547 650 216 121
Fragments arranged by size:
650 547 512 216 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 98 790 1929 2046
Size: 98 692 1139 117
Fragments arranged by size:
1139 692 117 98
EcoICRI GAG'CTC
Cuts at: 0 559 2046
Size: 559 1487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 157 810 1860 2046
Size: 28 129 653 1050 186
Fragments arranged by size:
1050 653 186 129 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1747 2046
Size: 1747 299
EcoO109I rG'GnC_Cy
Cuts at: 0 632 1265 1354 1422 1761 1821 2046
Size: 632 633 89 68 339 60 225
Fragments arranged by size:
633 632 339 225 89 68 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 167 1347 2046
Size: 167 1180 699
Fragments arranged by size:
1180 699 167
HaeII r_GCGC'y
Cuts at: 0 1570 1640 1706 1733 1779 1799 2046
Size: 1570 70 66 27 46 20 247
Fragments arranged by size:
1570 247 70 66 46 27 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 253 285 1936 1968 2046
Size: 165 32 56 32 1651 32 78
Fragments arranged by size:
1651 165 78 56 32 32 32
KasI G'GCGC_C
Cuts at: 0 1636 1729 1795 2046
Size: 1636 93 66 251
Fragments arranged by size:
1636 251 93 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 2046
Size: 509 1537
MslI CAynn'nnrTG
Cuts at: 0 838 1936 2046
Size: 838 1098 110
Fragments arranged by size:
1098 838 110
MspA1I CmG'CkG
Cuts at: 0 6 1575 1708 1785 1838 1983 2046
Size: 6 1569 133 77 53 145 63
Fragments arranged by size:
1569 145 133 77 63 53 6
NaeI GCC'GGC
Cuts at: 0 516 1284 1463 1728 1925 2046
Size: 516 768 179 265 197 121
Fragments arranged by size:
768 516 265 197 179 121
NarI GG'CG_CC
Cuts at: 0 1637 1730 1796 2046
Size: 1637 93 66 250
Fragments arranged by size:
1637 250 93 66
NcoI C'CATG_G
Cuts at: 0 1277 2046
Size: 1277 769
NgoMIV G'CCGG_C
Cuts at: 0 514 1282 1461 1726 1923 2046
Size: 514 768 179 265 197 123
Fragments arranged by size:
768 514 265 197 179 123
NotI GC'GGCC_GC
Cuts at: 0 1709 2046
Size: 1709 337
NsiI A_TGCA'T
Cuts at: 0 866 2046
Size: 866 1180
NspI r_CATG'y
Cuts at: 0 699 1015 2046
Size: 699 316 1031
Fragments arranged by size:
1031 699 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 788 2046
Size: 788 1258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 2046
Size: 1078 32 936
Fragments arranged by size:
1078 936 32
PpuMI rG'GwC_Cy
Cuts at: 0 1422 1821 2046
Size: 1422 399 225
Fragments arranged by size:
1422 399 225
PspOMI G'GGCC_C
Cuts at: 0 1265 1353 2046
Size: 1265 88 693
Fragments arranged by size:
1265 693 88
PstI C_TGCA'G
Cuts at: 0 306 1298 1580 1587 2046
Size: 306 992 282 7 459
Fragments arranged by size:
992 459 306 282 7
PvuII CAG'CTG
Cuts at: 0 6 1838 2046
Size: 6 1832 208
Fragments arranged by size:
1832 208 6
RsrII CG'GwC_CG
Cuts at: 0 391 1371 2046
Size: 391 980 675
Fragments arranged by size:
980 675 391
SacI G_AGCT'C
Cuts at: 0 561 2046
Size: 561 1485
SacII CC_GC'GG
Cuts at: 0 1709 2046
Size: 1709 337
SbfI CC_TGCA'GG
Cuts at: 0 1298 2046
Size: 1298 748
ScaI AGT'ACT
Cuts at: 0 1657 2046
Size: 1657 389
SfcI C'TryA_G
Cuts at: 0 302 1294 1576 1583 2046
Size: 302 992 282 7 463
Fragments arranged by size:
992 463 302 282 7
SfoI GGC'GCC
Cuts at: 0 1638 1731 1797 2046
Size: 1638 93 66 249
Fragments arranged by size:
1638 249 93 66
SmaI CCC'GGG
Cuts at: 0 1263 1340 1359 1427 2046
Size: 1263 77 19 68 619
Fragments arranged by size:
1263 619 77 68 19
SmlI C'TyrA_G
Cuts at: 0 1960 2046
Size: 1960 86
SspI AAT'ATT
Cuts at: 0 753 2046
Size: 753 1293
StyI C'CwwG_G
Cuts at: 0 212 552 1277 1322 1817 2006 2046
Size: 212 340 725 45 495 189 40
Fragments arranged by size:
725 495 340 212 189 45 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 598 2046
Size: 598 1448
TatI w'GTAC_w
Cuts at: 0 1655 2046
Size: 1655 391
Tth111I GACn'n_nGTC
Cuts at: 0 265 2046
Size: 265 1781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 1473 1740 2046
Size: 132 1341 267 306
Fragments arranged by size:
1341 306 267 132
XmaI C'CCGG_G
Cuts at: 0 1261 1338 1357 1425 2046
Size: 1261 77 19 68 621
Fragments arranged by size:
1261 621 77 68 19
ZraI GAC'GTC
Cuts at: 0 231 2046
Size: 231 1815
Enzymes that do cut:
AatII AfeI AleI ApaI ApoI AscI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BstAPI BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI
NspI PflMI PpiI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SbfI ScaI SfcI SfoI SmaI SmlI
SspI StyI TaqII TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AseI AsiSI AvrII
BbsI BbvCI BglII BlpI BmgBI BmtI Bpu10I BsaAI
BsiWI BsmI BspHI BspMI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NdeI NheI NruI
PacI PasI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI SalI SanDI SapI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TstI XbaI XhoI XmnI