(Circular) (Six-base) MAPSORT of: pFN21AB7338.seq Check: 4146 from: 1 to: 7894 pFN21AB7338 7894 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2522 4351 2522 Size: 1829 6065 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5704 278 Size: 53 83 186 936 4168 2468 Fragments arranged by size: 4168 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5237 1937 Size: 3300 4594 Acc65I G'GTAC_C Cuts at: 5215 5215 Size: 7894 AclI AA'CG_TT Cuts at: 1563 6021 6394 1563 Size: 4458 373 3063 Fragments arranged by size: 4458 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2521 3100 5957 7341 7609 1632 Size: 889 579 2857 1384 268 1917 Fragments arranged by size: 2857 1917 1384 889 579 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7697 AflIII A'CryG_T Cuts at: 1203 4729 6793 7770 1203 Size: 3526 2064 977 1327 Fragments arranged by size: 3526 2064 1327 977 AgeI A'CCGG_T Cuts at: 3457 6725 3457 Size: 3268 4626 AhdI GACnn_n'nnGTC Cuts at: 6623 6623 Size: 7894 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7894 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5061 5093 5061 Size: 32 7862 AlwNI CAG_nnn'CTG Cuts at: 2819 3484 5637 7209 2819 Size: 665 2153 1572 3504 Fragments arranged by size: 3504 2153 1572 665 ApaI G_GGCC'C Cuts at: 4870 4870 Size: 7894 ApaLI G'TGCA_C Cuts at: 5951 7107 5951 Size: 1156 6738 ApoI r'AATT_y Cuts at: 1493 2865 2884 2935 3050 3779 4556 4739 5203 Size: 1372 19 51 115 729 777 183 464 Cuts at: 5203 5429 5581 7690 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1372 777 729 464 226 183 152 115 51 19 AseI AT'TA_AT Cuts at: 160 1032 6448 160 Size: 872 5416 1606 Fragments arranged by size: 5416 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2031 4548 5219 1870 Size: 73 69 19 2517 671 4545 Fragments arranged by size: 4545 2517 671 73 69 19 BamHI G'GATC_C Cuts at: 5224 5591 5224 Size: 367 7527 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2233 2510 3028 3140 Size: 325 217 627 257 189 277 518 112 Cuts at: 3140 5215 6670 618 Size: 2075 1455 1842 Fragments arranged by size: 2075 1842 1455 627 518 325 277 257 217 189 112 BanII G_rGCy'C Cuts at: 1391 3107 4437 4674 4757 4870 5213 1391 Size: 1716 1330 237 83 113 343 4072 Fragments arranged by size: 4072 1716 1330 343 237 113 83 BbeI G_GCGC'C Cuts at: 2048 2237 2048 Size: 189 7705 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3697 3939 928 Size: 971 1798 242 4883 Fragments arranged by size: 4883 1798 971 242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2155 2189 4258 4292 6085 6119 1903 Size: 34 218 34 2069 34 1793 34 3678 Fragments arranged by size: 3678 2069 1793 218 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2801 3197 5786 6996 2801 Size: 396 2589 1210 3699 Fragments arranged by size: 3699 2589 1210 396 BclI T'GATC_A Cuts at: 1548 2688 1548 Size: 1140 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 2163 6505 Size: 107 122 71 631 605 442 49 4342 Cuts at: 6505 136 Size: 1525 Fragments arranged by size: 4342 1525 631 605 442 122 107 71 49 BglII A'GATC_T Cuts at: 7889 7889 Size: 7894 BlpI GC'TnA_GC Cuts at: 4673 5311 4673 Size: 638 7256 Bme1580I G_kGCm'C Cuts at: 1790 3031 4870 5955 7111 1790 Size: 1241 1839 1085 1156 2573 Fragments arranged by size: 2573 1839 1241 1156 1085 BmgBI CAC'GTC Cuts at: 1318 2131 4247 1318 Size: 813 2116 4965 Fragments arranged by size: 4965 2116 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2498 2844 4426 Size: 416 53 83 399 24 1081 346 1582 Cuts at: 4426 5625 6583 442 Size: 1199 958 1753 Fragments arranged by size: 1753 1582 1199 1081 958 416 399 346 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2491 2763 4544 4865 6554 1239 Size: 1252 272 1781 321 1689 2579 Fragments arranged by size: 2579 1781 1689 1252 321 272 Bpu10I CC'TnA_GC Cuts at: 4668 5095 4668 Size: 427 7467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4271 6019 6884 7182 4271 Size: 1748 865 298 4983 Fragments arranged by size: 4983 1748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2634 3114 3180 6557 882 Size: 617 1135 480 66 3377 2219 Fragments arranged by size: 3377 2219 1135 617 480 66 BsaAI yAC'GTr Cuts at: 493 1204 5685 493 Size: 711 4481 2702 Fragments arranged by size: 4481 2702 711 BsaBI GATnn'nnATC Cuts at: 3687 3981 4005 7888 3687 Size: 294 24 3883 3693 Fragments arranged by size: 3883 3693 294 24 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 2205 2234 5701 Size: 53 83 186 936 512 160 29 3467 Cuts at: 5701 6083 6718 275 Size: 382 635 1451 Fragments arranged by size: 3467 1451 936 635 512 382 186 160 83 53 29 BsaWI w'CCGG_w Cuts at: 2584 3347 3457 6327 6725 6999 7146 7557 2584 Size: 763 110 2870 398 274 147 411 2921 Fragments arranged by size: 2921 2870 763 411 398 274 147 110 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 3148 3178 1178 Size: 30 165 30 1003 30 712 30 5894 Fragments arranged by size: 5894 1003 712 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2889 3101 3384 4389 4445 4460 4463 4842 Size: 1703 212 283 1005 56 15 3 379 Cuts at: 4842 5127 1186 Size: 285 3953 Fragments arranged by size: 3953 1703 1005 379 285 283 212 56 15 3 BseYI C'CCAG_C Cuts at: 2083 2822 3477 7097 7593 2083 Size: 739 655 3620 496 2384 Fragments arranged by size: 3620 2384 739 655 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 3473 4469 4827 1163 Size: 573 605 1132 996 358 4230 Fragments arranged by size: 4230 1132 996 605 573 358 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 5344 6105 7133 1540 Size: 275 186 78 3265 761 1028 2301 Fragments arranged by size: 3265 2301 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2458 2881 3107 3264 4674 5955 6040 7111 2458 Size: 423 226 157 1410 1281 85 1071 3241 Fragments arranged by size: 3241 1410 1281 1071 423 226 157 85 BsmI GAATG_Cn' Cuts at: 5405 5498 5405 Size: 93 7801 BsmBI CGTCTCn'nnnn_ Cuts at: 3140 3140 Size: 7894 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2458 2881 3031 3107 3264 4437 4674 Size: 399 668 423 150 76 157 1173 237 Cuts at: 4674 4757 4870 5213 5955 6040 7111 1391 Size: 83 113 343 742 85 1071 2174 Fragments arranged by size: 2174 1173 1071 742 668 423 399 343 237 157 150 113 85 83 76 BspEI T'CCGG_A Cuts at: 2584 3347 7557 2584 Size: 763 4210 2921 Fragments arranged by size: 4210 2921 763 BspHI T'CATG_A Cuts at: 5783 7513 5783 Size: 1730 6164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2522 4351 5249 844 Size: 1678 1829 898 3489 Fragments arranged by size: 3489 1829 1678 898 BsrBI CCG'CTC Cuts at: 1394 1823 5781 1394 Size: 429 3958 3507 Fragments arranged by size: 3958 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2581 2712 3213 3597 6389 6563 65 Size: 1183 1333 131 501 384 2792 174 1396 Fragments arranged by size: 2792 1396 1333 1183 501 384 174 131 BsrFI r'CCGG_y Cuts at: 1700 1810 3457 6538 6725 1700 Size: 110 1647 3081 187 2869 Fragments arranged by size: 3081 2869 1647 187 110 BsrGI T'GTAC_A Cuts at: 96 2148 4726 4775 96 Size: 2052 2578 49 3215 Fragments arranged by size: 3215 2578 2052 49 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7894 BssSI C'ACGA_G Cuts at: 5954 6966 5954 Size: 1012 6882 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7894 BstBI TT'CG_AA Cuts at: 6698 6698 Size: 7894 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7894 BstXI CCAn_nnnn'nTGG Cuts at: 4174 4174 Size: 7894 BstYI r'GATC_y Cuts at: 1972 3129 5224 5591 5976 5993 7445 7531 7543 Size: 1157 2095 367 385 17 1452 86 12 Cuts at: 7543 7889 1972 Size: 346 1977 Fragments arranged by size: 2095 1977 1452 1157 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2741 5139 5187 2741 Size: 2398 48 5448 Fragments arranged by size: 5448 2398 48 BtgI C'CryG_G Cuts at: 513 1065 2023 2073 2184 2301 513 Size: 552 958 50 111 117 6106 Fragments arranged by size: 6106 958 552 117 111 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3683 487 Size: 683 56 2457 4698 Fragments arranged by size: 4698 2457 683 56 BtsI GCAGTG_nn' Cuts at: 2324 2691 4347 4607 4815 5410 6204 6224 2324 Size: 367 1656 260 208 595 794 20 3994 Fragments arranged by size: 3994 1656 794 595 367 260 208 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7894 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3305 3340 529 Size: 35 2741 35 5083 Fragments arranged by size: 5083 2741 35 35 DraI TTT'AAA Cuts at: 3844 5198 3844 Size: 1354 6540 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6901 809 Size: 507 5585 1802 Fragments arranged by size: 5585 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 5341 6230 Size: 54 1425 50 275 18 246 3265 889 Cuts at: 6230 7859 8 Size: 1629 43 Fragments arranged by size: 3265 1629 1425 889 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 5341 1537 Size: 275 264 3265 4090 Fragments arranged by size: 4090 3265 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 3119 3680 4080 4422 4712 5824 1353 Size: 1766 561 400 342 290 1112 3423 Fragments arranged by size: 3423 1766 1112 561 400 342 290 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6481 6855 7001 387 Size: 1064 138 4892 374 146 1280 Fragments arranged by size: 4892 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3105 4672 3105 Size: 1567 6327 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2491 2521 2763 3100 4544 4865 5957 Size: 393 859 30 242 337 1444 321 1092 Cuts at: 5957 6554 7341 7609 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1444 1092 859 787 597 393 337 321 268 242 30 EcoNI CCTnn'n_nnAGG Cuts at: 3234 3234 Size: 7894 EcoO109I rG'GnC_Cy Cuts at: 2500 2657 4592 2500 Size: 157 1935 5802 Fragments arranged by size: 5802 1935 157 EcoRI G'AATT_C Cuts at: 2865 3779 4739 5203 5581 2865 Size: 914 960 464 378 5178 Fragments arranged by size: 5178 960 914 464 378 EcoRV GAT'ATC Cuts at: 2263 2710 2737 2263 Size: 447 27 7420 Fragments arranged by size: 7420 447 27 FspI TGC'GCA Cuts at: 2316 6400 2316 Size: 4084 3810 HaeII r_GCGC'y Cuts at: 1678 2048 2237 2544 4680 7041 1678 Size: 370 189 307 2136 2361 2531 Fragments arranged by size: 2531 2361 2136 370 307 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2673 2705 3490 Size: 32 135 32 809 32 260 32 785 Cuts at: 3490 3522 3779 3811 6535 6567 6609 6641 1373 Size: 32 257 32 2724 32 42 32 2626 Fragments arranged by size: 2724 2626 809 785 260 257 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2995 5238 5484 669 Size: 988 281 1057 2243 246 3079 Fragments arranged by size: 3079 2243 1057 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7894 HpaI GTT'AAC Cuts at: 2995 5484 2995 Size: 2489 5405 KasI G'GCGC_C Cuts at: 2044 2233 2044 Size: 189 7705 KpnI G_GTAC'C Cuts at: 5219 5219 Size: 7894 MfeI C'AATT_G Cuts at: 5493 5493 Size: 7894 MluI A'CGCG_T Cuts at: 7770 7770 Size: 7894 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4081 4323 4435 7008 7192 1305 Size: 2776 242 112 2573 184 2007 Fragments arranged by size: 2776 2573 2007 242 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6072 Fragments arranged by size: 6072 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3224 3917 4476 4861 5852 6211 6370 Size: 648 2058 693 559 385 991 359 159 Cuts at: 6370 518 Size: 2042 Fragments arranged by size: 2058 2042 991 693 648 559 385 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2050 2075 2154 2303 5167 5310 5987 Size: 419 25 25 79 149 2864 143 677 Cuts at: 5987 7135 7380 1606 Size: 1148 245 2120 Fragments arranged by size: 2864 2120 1148 677 419 245 149 143 79 25 25 NarI GG'CG_CC Cuts at: 2045 2234 2045 Size: 189 7705 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7342 NdeI CA'TA_TG Cuts at: 387 4054 387 Size: 3667 4227 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7894 NotI GC'GGCC_GC Cuts at: 2076 5341 2076 Size: 3265 4629 NspI r_CATG'y Cuts at: 1132 1229 3338 4733 5252 6797 1132 Size: 97 2109 1395 519 1545 2229 Fragments arranged by size: 2229 2109 1545 1395 519 97 PasI CC'CwG_GG Cuts at: 1348 4222 1348 Size: 2874 5020 PciI A'CATG_T Cuts at: 4729 6793 4729 Size: 2064 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2406 1108 Size: 1298 6596 PfoI T'CCnGG_A Cuts at: 4521 4521 Size: 7894 PmeI GTTT'AAAC Cuts at: 5198 5198 Size: 7894 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5963 5995 7476 7508 5963 Size: 32 1481 32 6349 Fragments arranged by size: 6349 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2500 2657 4592 2500 Size: 157 1935 5802 Fragments arranged by size: 5802 1935 157 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7894 PsiI TTA'TAA Cuts at: 2631 3270 5464 2631 Size: 639 2194 5061 Fragments arranged by size: 5061 2194 639 PspOMI G'GGCC_C Cuts at: 4866 4866 Size: 7894 PstI C_TGCA'G Cuts at: 830 4602 5246 830 Size: 3772 644 3478 Fragments arranged by size: 3772 3478 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7894 PvuII CAG'CTG Cuts at: 5167 5167 Size: 7894 SacI G_AGCT'C Cuts at: 3107 4674 3107 Size: 1567 6327 SacII CC_GC'GG Cuts at: 2026 2076 2304 2026 Size: 50 228 7616 Fragments arranged by size: 7616 228 50 SalI G'TCGA_C Cuts at: 1936 5236 1936 Size: 3300 4594 SapI GCTCTTCn'nnn_ Cuts at: 3680 3680 Size: 7894 SbfI CC_TGCA'GG Cuts at: 5246 5246 Size: 7894 ScaI AGT'ACT Cuts at: 6142 7854 6142 Size: 1712 6182 SfcI C'TryA_G Cuts at: 826 1045 3468 3880 4353 4598 5242 6377 7058 Size: 219 2423 412 473 245 644 1135 681 Cuts at: 7058 7249 826 Size: 191 1471 Fragments arranged by size: 2423 1471 1135 681 644 473 412 245 219 191 SfoI GGC'GCC Cuts at: 2046 2235 2046 Size: 189 7705 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7894 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7894 SmaI CCC'GGG Cuts at: 1872 2014 2033 4550 5221 1872 Size: 142 19 2517 671 4545 Fragments arranged by size: 4545 2517 671 142 19 SmlI C'TyrA_G Cuts at: 820 1017 1943 4250 5998 6899 7161 820 Size: 197 926 2307 1748 901 262 1553 Fragments arranged by size: 2307 1748 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5685 493 Size: 5192 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7894 SphI G_CATG'C Cuts at: 1132 5252 1132 Size: 4120 3774 SspI AAT'ATT Cuts at: 5 52 3870 4492 5818 5 Size: 47 3818 622 1326 2081 Fragments arranged by size: 3818 2081 1326 622 47 StuI AGG'CCT Cuts at: 1517 3852 1517 Size: 2335 5559 StyI C'CwwG_G Cuts at: 513 1065 2160 2294 2803 4186 5333 513 Size: 552 1095 134 509 1383 1147 3074 Fragments arranged by size: 3074 1383 1147 1095 552 509 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3507 5168 5921 5938 6091 6276 Size: 1255 1824 103 1661 753 17 153 185 Cuts at: 6276 6721 325 Size: 445 1498 Fragments arranged by size: 1824 1661 1498 1255 753 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 4146 4726 Size: 274 80 33 51 1443 171 1998 580 Cuts at: 4726 4775 6140 7852 96 Size: 49 1365 1712 138 Fragments arranged by size: 1998 1712 1443 1365 580 274 171 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2430 2438 2462 3324 3356 4449 4481 7854 Size: 24 8 24 862 32 1093 32 3373 Cuts at: 7854 7886 2406 Size: 32 2414 Fragments arranged by size: 3373 2414 1093 862 32 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7894 XbaI T'CTAG_A Cuts at: 5230 5230 Size: 7894 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3134 4525 3134 Size: 1391 6503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7894 XmaI C'CCGG_G Cuts at: 1870 2012 2031 4548 5219 1870 Size: 142 19 2517 671 4545 Fragments arranged by size: 4545 2517 671 142 19 XmnI GAAnn'nnTTC Cuts at: 4560 6023 4560 Size: 1463 6431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5702 276 Size: 53 83 186 936 4168 2468 Fragments arranged by size: 4168 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI