Restriction map for
pFN21AB7338
(Linear) (Six-base) MAPSORT of: KIBB7338.seq Check: 4985 from: 1 to: 3189
KIBB7338.orf
With 182 enzymes: SgfI *
July 22, 2009 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 517 2346 3189
Size: 517 1829 843
Fragments arranged by size:
1829 843 517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 1095 3189
Size: 516 579 2094
Fragments arranged by size:
2094 579 516
AflIII A'CryG_T
Cuts at: 0 2724 3189
Size: 2724 465
AgeI A'CCGG_T
Cuts at: 0 1452 3189
Size: 1452 1737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3056 3088 3189
Size: 3056 32 101
Fragments arranged by size:
3056 101 32
AlwNI CAG_nnn'CTG
Cuts at: 0 814 1479 3189
Size: 814 665 1710
Fragments arranged by size:
1710 814 665
ApaI G_GGCC'C
Cuts at: 0 2865 3189
Size: 2865 324
ApoI r'AATT_y
Cuts at: 0 860 879 930 1045 1774 2551 2734 3189
Size: 860 19 51 115 729 777 183 455
Fragments arranged by size:
860 777 729 455 183 115 51 19
AvaI C'yCGr_G
Cuts at: 0 7 26 2543 3189
Size: 7 19 2517 646
Fragments arranged by size:
2517 646 19 7
BanI G'GyrC_C
Cuts at: 0 39 228 505 1023 1135 3189
Size: 39 189 277 518 112 2054
Fragments arranged by size:
2054 518 277 189 112 39
BanII G_rGCy'C
Cuts at: 0 1102 2432 2669 2752 2865 3189
Size: 1102 1330 237 83 113 324
Fragments arranged by size:
1330 1102 324 237 113 83
BbeI G_GCGC'C
Cuts at: 0 43 232 3189
Size: 43 189 2957
Fragments arranged by size:
2957 189 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 1692 1934 3189
Size: 1692 242 1255
Fragments arranged by size:
1692 1255 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 2253 2287 3189
Size: 150 34 2069 34 902
Fragments arranged by size:
2069 902 150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 796 1192 3189
Size: 796 396 1997
Fragments arranged by size:
1997 796 396
BclI T'GATC_A
Cuts at: 0 683 3189
Size: 683 2506
BglI GCCn_nnn'nGGC
Cuts at: 0 109 158 3189
Size: 109 49 3031
Fragments arranged by size:
3031 109 49
BlpI GC'TnA_GC
Cuts at: 0 2668 3189
Size: 2668 521
Bme1580I G_kGCm'C
Cuts at: 0 1026 2865 3189
Size: 1026 1839 324
Fragments arranged by size:
1839 1026 324
BmgBI CAC'GTC
Cuts at: 0 126 2242 3189
Size: 126 2116 947
Fragments arranged by size:
2116 947 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 839 2421 3189
Size: 493 346 1582 768
Fragments arranged by size:
1582 768 493 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 758 2539 2860 3189
Size: 486 272 1781 321 329
Fragments arranged by size:
1781 486 329 321 272
Bpu10I CC'TnA_GC
Cuts at: 0 2663 3090 3189
Size: 2663 427 99
Fragments arranged by size:
2663 427 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2266 3189
Size: 2266 923
BsaI GGTCTCn'nnnn_
Cuts at: 0 629 1109 1175 3189
Size: 629 480 66 2014
Fragments arranged by size:
2014 629 480 66
BsaBI GATnn'nnATC
Cuts at: 0 1682 1976 2000 3189
Size: 1682 294 24 1189
Fragments arranged by size:
1682 1189 294 24
BsaHI Gr'CG_yC
Cuts at: 0 40 200 229 3189
Size: 40 160 29 2960
Fragments arranged by size:
2960 160 40 29
BsaWI w'CCGG_w
Cuts at: 0 579 1342 1452 3189
Size: 579 763 110 1737
Fragments arranged by size:
1737 763 579 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 1143 1173 3189
Size: 401 30 712 30 2016
Fragments arranged by size:
2016 712 401 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 884 1096 1379 2384 2440 2455 2458 2837
Size: 884 212 283 1005 56 15 3 379
Cuts at: 2837 3122 3189
Size: 285 67
Fragments arranged by size:
1005 884 379 285 283 212 67 56
15 3
BseYI C'CCAG_C
Cuts at: 0 78 817 1472 3189
Size: 78 739 655 1717
Fragments arranged by size:
1717 739 655 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 1468 2464 2822 3189
Size: 336 1132 996 358 367
Fragments arranged by size:
1132 996 367 358 336
BsiEI CG_ry'CG
Cuts at: 0 74 3189
Size: 74 3115
BsiHKAI G_wGCw'C
Cuts at: 0 453 876 1102 1259 2669 3189
Size: 453 423 226 157 1410 520
Fragments arranged by size:
1410 520 453 423 226 157
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1135 3189
Size: 1135 2054
Bsp1286I G_dGCh'C
Cuts at: 0 453 876 1026 1102 1259 2432 2669 2752
Size: 453 423 150 76 157 1173 237 83
Cuts at: 2752 2865 3189
Size: 113 324
Fragments arranged by size:
1173 453 423 324 237 157 150 113
83 76
BspEI T'CCGG_A
Cuts at: 0 579 1342 3189
Size: 579 763 1847
Fragments arranged by size:
1847 763 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 2346 3189
Size: 517 1829 843
Fragments arranged by size:
1829 843 517
BsrDI GCAATG_nn'
Cuts at: 0 576 707 1208 1592 3189
Size: 576 131 501 384 1597
Fragments arranged by size:
1597 576 501 384 131
BsrFI r'CCGG_y
Cuts at: 0 1452 3189
Size: 1452 1737
BsrGI T'GTAC_A
Cuts at: 0 143 2721 2770 3189
Size: 143 2578 49 419
Fragments arranged by size:
2578 419 143 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2169 3189
Size: 2169 1020
BstYI r'GATC_y
Cuts at: 0 1124 3189
Size: 1124 2065
Bsu36I CC'TnA_GG
Cuts at: 0 736 3134 3182 3189
Size: 736 2398 48 7
Fragments arranged by size:
2398 736 48 7
BtgI C'CryG_G
Cuts at: 0 18 68 179 296 3189
Size: 18 50 111 117 2893
Fragments arranged by size:
2893 117 111 50 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1678 3189
Size: 1678 1511
BtsI GCAGTG_nn'
Cuts at: 0 319 686 2342 2602 2810 3189
Size: 319 367 1656 260 208 379
Fragments arranged by size:
1656 379 367 319 260 208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1300 1335 3189
Size: 1300 35 1854
Fragments arranged by size:
1854 1300 35
DraI TTT'AAA
Cuts at: 0 1839 3189
Size: 1839 1350
EaeI y'GGCC_r
Cuts at: 0 71 3189
Size: 71 3118
EagI C'GGCC_G
Cuts at: 0 71 3189
Size: 71 3118
EarI CTCTTCn'nnn_
Cuts at: 0 1114 1675 2075 2417 2707 3189
Size: 1114 561 400 342 290 482
Fragments arranged by size:
1114 561 482 400 342 290
EcoICRI GAG'CTC
Cuts at: 0 1100 2667 3189
Size: 1100 1567 522
Fragments arranged by size:
1567 1100 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 486 516 758 1095 2539 2860 3189
Size: 486 30 242 337 1444 321 329
Fragments arranged by size:
1444 486 337 329 321 242 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1229 3189
Size: 1229 1960
EcoO109I rG'GnC_Cy
Cuts at: 0 495 652 2587 3189
Size: 495 157 1935 602
Fragments arranged by size:
1935 602 495 157
EcoRI G'AATT_C
Cuts at: 0 860 1774 2734 3189
Size: 860 914 960 455
Fragments arranged by size:
960 914 860 455
EcoRV GAT'ATC
Cuts at: 0 258 705 732 3189
Size: 258 447 27 2457
Fragments arranged by size:
2457 447 258 27
FspI TGC'GCA
Cuts at: 0 311 3189
Size: 311 2878
HaeII r_GCGC'y
Cuts at: 0 43 232 539 2675 3189
Size: 43 189 307 2136 514
Fragments arranged by size:
2136 514 307 189 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 668 700 1485 1517 1774 1806
Size: 376 32 260 32 785 32 257 32
Cuts at: 1806 3189
Size: 1383
Fragments arranged by size:
1383 785 376 260 257 32 32 32
32
HincII GTy'rAC
Cuts at: 0 990 3189
Size: 990 2199
HpaI GTT'AAC
Cuts at: 0 990 3189
Size: 990 2199
KasI G'GCGC_C
Cuts at: 0 39 228 3189
Size: 39 189 2961
Fragments arranged by size:
2961 189 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2076 2318 2430 3189
Size: 2076 242 112 759
Fragments arranged by size:
2076 759 242 112
MslI CAynn'nnrTG
Cuts at: 0 1219 1912 2471 2856 3189
Size: 1219 693 559 385 333
Fragments arranged by size:
1219 693 559 385 333
MspA1I CmG'CkG
Cuts at: 0 20 45 70 149 298 3162 3189
Size: 20 25 25 79 149 2864 27
Fragments arranged by size:
2864 149 79 27 25 25 20
NarI GG'CG_CC
Cuts at: 0 40 229 3189
Size: 40 189 2960
Fragments arranged by size:
2960 189 40
NdeI CA'TA_TG
Cuts at: 0 2049 3189
Size: 2049 1140
NotI GC'GGCC_GC
Cuts at: 0 71 3189
Size: 71 3118
NspI r_CATG'y
Cuts at: 0 1333 2728 3189
Size: 1333 1395 461
Fragments arranged by size:
1395 1333 461
PasI CC'CwG_GG
Cuts at: 0 2217 3189
Size: 2217 972
PciI A'CATG_T
Cuts at: 0 2724 3189
Size: 2724 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 401 3189
Size: 401 2788
PfoI T'CCnGG_A
Cuts at: 0 2516 3189
Size: 2516 673
PpuMI rG'GwC_Cy
Cuts at: 0 495 652 2587 3189
Size: 495 157 1935 602
Fragments arranged by size:
1935 602 495 157
PsiI TTA'TAA
Cuts at: 0 626 1265 3189
Size: 626 639 1924
Fragments arranged by size:
1924 639 626
PspOMI G'GGCC_C
Cuts at: 0 2861 3189
Size: 2861 328
PstI C_TGCA'G
Cuts at: 0 2597 3189
Size: 2597 592
PvuII CAG'CTG
Cuts at: 0 3162 3189
Size: 3162 27
SacI G_AGCT'C
Cuts at: 0 1102 2669 3189
Size: 1102 1567 520
Fragments arranged by size:
1567 1102 520
SacII CC_GC'GG
Cuts at: 0 21 71 299 3189
Size: 21 50 228 2890
Fragments arranged by size:
2890 228 50 21
SapI GCTCTTCn'nnn_
Cuts at: 0 1675 3189
Size: 1675 1514
SfcI C'TryA_G
Cuts at: 0 1463 1875 2348 2593 3189
Size: 1463 412 473 245 596
Fragments arranged by size:
1463 596 473 412 245
SfoI GGC'GCC
Cuts at: 0 41 230 3189
Size: 41 189 2959
Fragments arranged by size:
2959 189 41
SmaI CCC'GGG
Cuts at: 0 9 28 2545 3189
Size: 9 19 2517 644
Fragments arranged by size:
2517 644 19 9
SmlI C'TyrA_G
Cuts at: 0 2245 3189
Size: 2245 944
SspI AAT'ATT
Cuts at: 0 1865 2487 3189
Size: 1865 622 702
Fragments arranged by size:
1865 702 622
StuI AGG'CCT
Cuts at: 0 1847 3189
Size: 1847 1342
StyI C'CwwG_G
Cuts at: 0 155 289 798 2181 3189
Size: 155 134 509 1383 1008
Fragments arranged by size:
1383 1008 509 155 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1399 1502 3163 3189
Size: 1399 103 1661 26
Fragments arranged by size:
1661 1399 103 26
TatI w'GTAC_w
Cuts at: 0 143 2141 2721 2770 3189
Size: 143 1998 580 49 419
Fragments arranged by size:
1998 580 419 143 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 425 433 457 1319 1351 2444 2476
Size: 401 24 8 24 862 32 1093 32
Cuts at: 2476 3189
Size: 713
Fragments arranged by size:
1093 862 713 401 32 32 24 24
8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1129 2520 3189
Size: 1129 1391 669
Fragments arranged by size:
1391 1129 669
XmaI C'CCGG_G
Cuts at: 0 7 26 2543 3189
Size: 7 19 2517 646
Fragments arranged by size:
2517 646 19 7
XmnI GAAnn'nnTTC
Cuts at: 0 2555 3189
Size: 2555 634
Enzymes that do cut:
AarI AcuI AflIII AgeI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BsrGI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HpaI KasI
MmeI MslI MspA1I NarI NdeI NotI NspI PasI
PciI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII
SacI SacII SapI SfcI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BfrBI BglII BmtI BplI BsaAI BsiWI BsmI
BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I
ClaI DraIII DrdI EciI FalI FseI FspAI HindIII
KpnI MfeI MluI MscI NaeI NcoI NgoMIV NheI
NruI NsiI PacI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI ZraI