Restriction map for
pFN21AB5776
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
AspEI AspHI AspI AviII AvrII
BalI BbrPI BfrI BlnI BsaAI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FseI
FspI HindIII HpaI MluI MluNI
MscI NcoI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PstI PvuI
PvuII RsrII SacI SalI SapI
SbfI SexAI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 504 GGTACC G^GTACC
AccIII 129 TCCGGA T^CCGGA
AclI 720 AACGTT AA^CGTT
ApaI 979 GGGCCC GGGCC^C
AseI 2073 ATTAAT AT^TAAT
AsnI 2073 ATTAAT AT^TAAT
Asp718I 504 GGTACC G^GTACC
BamHI 1843 GGATCC G^GATCC
BcgI 958 GCACCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1649 TGATCA T^GATCA
BcnI 40 CCGGG CC^[GC]GG
BglI 103 GCCTCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2028 AGATCT A^GATCT
BlpI 9 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
BsaWI 129 TCCGGA [AT]^CCGG[AT]
BseAI 129 TCCGGA T^CCGGA
BseRI 133 GAGGAG GAGGAG(10/8)
BsgI 1679 CTGCAC GTGCAG(16/14)
BsmBI 1001 CGTCTC CGTCTC(1/5)
BsmI 1877 GAATGC GAATGC(1/-1)
BspEI 129 TCCGGA T^CCGGA
BspHI 1752 TCATGA T^CATGA
BspMI 507 ACCTGC ACCTGC(4/8)
BsrDI 2159 CATTGC GCAATG(2/0)
BstAPI 922 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 9 GCTGAGC GC^T[ACGT]AGC
DsaI 98 CCGCGG C^C[GA][CT]GG
EcoRI 596 GAATTC G^AATTC
EcoT14I 1155 CCAAGG C^C[AT][AT]GG
EcoT22I 1919 ATGCAT ATGCA^T
FbaI 1649 TGATCA T^GATCA
HgaI 913 GCGTC GACGC(5/10)
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
KpnI 504 GGTACC GGTAC^C
KspI 98 CCGCGG CCGC^GG
MfeI 620 CAATTG C^AATTG
MroI 129 TCCGGA T^CCGGA
MunI 620 CAATTG C^AATTG
NciI 40 CCGGG CC^[GC]GG
NsiI 1919 ATGCAT ATGCA^T
PaeR7I 1335 CTCGAG C^TCGAG
PflMI 1847 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 745 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2073 ATTAAT AT^TAAT
Psp1406I 720 AACGTT AA^CGTT
RcaI 1752 TCATGA T^CATGA
SacII 98 CCGCGG CCGC^GG
ScaI 1509 AGTACT AGT^ACT
SfcI 1793 CTACAG C^T[GA][CT]AG
SspI 1132 AATATT AAT^ATT
StuI 239 AGGCCT AGG^CCT
StyI 1155 CCAAGG C^C[AT][AT]GG
Van91I 1847 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1335 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1325 GTAGAC GT^[AC][GT]AC
1411 GTCTAC
AccII 33 CGCG CG^CG
99 CGCG
AflIII 787 ACATGT A^C[GA][CT]GT
1527 ACATGT
AlwNI 46 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
923 CAGAGACTG
AvaI 73 CCCGAG C^[CT]CG[GA]G
1335 CTCGAG
BbvCI 1264 CCTCAGC CCTCAGC(-5/-2)
1474 CCTCAGC
BciVI 351 GGATAC GTATCC(6/5)
2014 GGATAC
BsoBI 73 CCCGAG C^[CT]CG[GA]G
1335 CTCGAG
BspLU11I 787 ACATGT A^CATGT
1527 ACATGT
BsrBI 2154 CCGCTC CCGCTC(-3/-3)
12 GAGCGG
BstUI 33 CGCG CG^CG
99 CGCG
BstXI 422 CCACAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
619 CCAATTGGTTGG
FauI 961 CCCGC CCCGC(4/6)
2153 CCCGC
MaeII 721 ACGT A^CGT
1235 ACGT
MspA1I 98 CCGCGG C[AC]G^C[GT]G
777 CCGCTG
MvnI 33 CGCG CG^CG
99 CGCG
PpuMI 1260 AGGACCT [GA]G^G[AT]CC[CT]
1816 GGGTCCC
SgrAI 84 CGCCGGCG C[GA]^CCGG[CT]G
90 CGCCGGCG
SmlI 1335 CTCGAG C^T[CT][GA]AG
1729 CTCAAG
XcmI 1155 CCAAGGATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1847 CCATTTTGTGGATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BanI BanII
BbeI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NaeI NarI
NdeII NgoMIV NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII