(Circular) (Six-base) MAPSORT of: pFN21AB5776.seq Check: 1715 from: 1 to: 7003 pFN21AB5776 7003 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3331 3417 4346 1937 Size: 1394 86 929 4594 Fragments arranged by size: 4594 1394 929 86 Acc65I G'GTAC_C Cuts at: 2509 4324 2509 Size: 1815 5188 AclI AA'CG_TT Cuts at: 1563 2726 5130 5503 1563 Size: 1163 2404 373 3063 Fragments arranged by size: 3063 2404 1163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5066 6450 6718 1632 Size: 3434 1384 268 1917 Fragments arranged by size: 3434 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6806 AflIII A'CryG_T Cuts at: 1203 2792 3532 5902 6879 1203 Size: 1589 740 2370 977 1327 Fragments arranged by size: 2370 1589 1327 977 740 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 2432 2839 4182 1166 Size: 1266 407 1343 3987 Fragments arranged by size: 3987 1343 1266 407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7003 AlwNI CAG_nnn'CTG Cuts at: 2056 2933 4746 6318 2056 Size: 877 1813 1572 2741 Fragments arranged by size: 2741 1813 1572 877 ApaI G_GGCC'C Cuts at: 2988 2988 Size: 7003 ApaLI G'TGCA_C Cuts at: 5060 6216 5060 Size: 1156 5847 ApoI r'AATT_y Cuts at: 1493 2204 2601 2804 2991 3644 4131 4173 4312 Size: 711 397 203 187 653 487 42 139 Cuts at: 4312 4538 4690 6799 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 653 487 397 226 203 187 152 139 42 AseI AT'TA_AT Cuts at: 160 1032 4079 5557 160 Size: 872 3047 1478 1606 Fragments arranged by size: 3047 1606 1478 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 2078 3340 4328 1870 Size: 73 135 1262 988 4545 Fragments arranged by size: 4545 1262 988 135 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2493 2526 2493 Size: 33 6970 BamHI G'GATC_C Cuts at: 3848 4333 4700 3848 Size: 485 367 6151 Fragments arranged by size: 6151 485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2093 2099 2509 3865 Size: 325 217 627 300 6 6 410 1356 Cuts at: 3865 4324 5779 618 Size: 459 1455 1842 Fragments arranged by size: 1842 1455 1356 627 459 410 325 300 217 6 6 BanII G_rGCy'C Cuts at: 1391 2079 2988 3608 4322 1391 Size: 688 909 620 714 4072 Fragments arranged by size: 4072 909 714 688 620 BbeI G_GCGC'C Cuts at: 2091 2097 2103 2091 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2473 3309 3768 928 Size: 971 574 836 459 4163 Fragments arranged by size: 4163 971 836 574 459 BbvCI CC'TCA_GC Cuts at: 3270 3480 3270 Size: 210 6793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2952 2986 5194 5228 1903 Size: 34 1015 34 2208 34 3678 Fragments arranged by size: 3678 2208 1015 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2350 4013 4895 6105 2350 Size: 1663 882 1210 3248 Fragments arranged by size: 3248 1663 1210 882 BclI T'GATC_A Cuts at: 1548 3654 1548 Size: 2106 4897 BfrBI ATG'CAT Cuts at: 3926 3926 Size: 7003 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2114 5614 Size: 107 122 71 631 605 337 105 3500 Cuts at: 5614 136 Size: 1525 Fragments arranged by size: 3500 1525 631 605 337 122 107 105 71 BglII A'GATC_T Cuts at: 4033 6998 4033 Size: 2965 4038 BlpI GC'TnA_GC Cuts at: 2015 4420 2015 Size: 2405 4598 Bme1580I G_kGCm'C Cuts at: 1790 2988 5064 6220 1790 Size: 1198 2076 1156 2573 Fragments arranged by size: 2573 2076 1198 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7003 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3586 4734 5692 Size: 416 53 83 399 24 2169 1148 958 Cuts at: 5692 442 Size: 1753 Fragments arranged by size: 2169 1753 1148 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2679 3246 3592 4014 5663 1239 Size: 1022 418 567 346 422 1649 2579 Fragments arranged by size: 2579 1649 1022 567 422 418 346 Bpu10I CC'TnA_GC Cuts at: 2737 2923 3270 3480 2737 Size: 186 347 210 6260 Fragments arranged by size: 6260 347 210 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3719 5128 5993 6291 3719 Size: 1409 865 298 4431 Fragments arranged by size: 4431 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3078 3150 3632 3723 4108 5666 882 Size: 617 1579 72 482 91 385 1558 2219 Fragments arranged by size: 2219 1579 1558 617 482 385 91 72 BsaAI yAC'GTr Cuts at: 493 1204 4794 493 Size: 711 3590 2702 Fragments arranged by size: 3590 2702 711 BsaBI GATnn'nnATC Cuts at: 3057 3094 3400 3894 6997 3057 Size: 37 306 494 3103 3063 Fragments arranged by size: 3103 3063 494 306 37 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2094 2100 2918 Size: 53 83 186 936 555 6 6 818 Cuts at: 2918 4810 5192 5827 275 Size: 1892 382 635 1451 Fragments arranged by size: 1892 1451 936 818 635 555 382 186 83 53 6 6 BsaWI w'CCGG_w Cuts at: 2134 5436 5834 6108 6255 6666 2134 Size: 3302 398 274 147 411 2471 Fragments arranged by size: 3302 2471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2517 2547 4042 Size: 30 165 30 726 30 358 30 1495 Cuts at: 4042 4072 1178 Size: 30 4109 Fragments arranged by size: 4109 1495 726 358 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 1186 Size: 967 6036 BseYI C'CCAG_C Cuts at: 6206 6702 6206 Size: 496 6507 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3669 1163 Size: 573 1933 4497 Fragments arranged by size: 4497 1933 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4453 5214 6242 1540 Size: 275 186 2452 761 1028 2301 Fragments arranged by size: 2452 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5064 5149 6220 5064 Size: 85 1071 5847 Fragments arranged by size: 5847 1071 85 BsmI GAATG_Cn' Cuts at: 3888 4514 4607 3888 Size: 626 93 6284 Fragments arranged by size: 6284 626 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3012 3012 Size: 7003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2988 3608 4322 5064 5149 6220 Size: 399 289 909 620 714 742 85 1071 Cuts at: 6220 1391 Size: 2174 Fragments arranged by size: 2174 1071 909 742 714 620 399 289 85 BspEI T'CCGG_A Cuts at: 2134 6666 2134 Size: 4532 2471 BspHI T'CATG_A Cuts at: 3757 4892 6622 3757 Size: 1135 1730 4138 Fragments arranged by size: 4138 1730 1135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2521 4358 844 Size: 1677 1837 3489 Fragments arranged by size: 3489 1837 1677 BsrBI CCG'CTC Cuts at: 1394 1823 2019 4161 4890 1394 Size: 429 196 2142 729 3507 Fragments arranged by size: 3507 2142 729 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 5498 5672 65 Size: 1183 2915 1335 174 1396 Fragments arranged by size: 2915 1396 1335 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 2090 2096 5647 5834 1700 Size: 110 274 6 6 3551 187 2869 Fragments arranged by size: 3551 2869 274 187 110 6 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7003 BssSI C'ACGA_G Cuts at: 5063 6075 5063 Size: 1012 5991 BstAPI GCAn_nnn'nTGC Cuts at: 794 2933 794 Size: 2139 4864 BstBI TT'CG_AA Cuts at: 5807 5807 Size: 7003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7003 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2631 2434 Size: 197 6806 BstYI r'GATC_y Cuts at: 1972 3395 3848 4033 4333 4700 5085 5102 6554 Size: 1423 453 185 300 367 385 17 1452 Cuts at: 6554 6640 6652 6998 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1423 453 385 367 346 300 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2103 513 Size: 552 939 99 5413 Fragments arranged by size: 5413 939 552 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6264 Fragments arranged by size: 6264 683 56 BtsI GCAGTG_nn' Cuts at: 3453 4189 4519 5313 5333 3453 Size: 736 330 794 20 5123 Fragments arranged by size: 5123 794 736 330 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2191 2226 529 Size: 35 1627 35 5306 Fragments arranged by size: 5306 1627 35 35 DraI TTT'AAA Cuts at: 4307 4307 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6010 809 Size: 507 4694 1802 Fragments arranged by size: 4694 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4450 5339 6968 Size: 54 1425 50 275 18 2620 889 1629 Cuts at: 6968 8 Size: 43 Fragments arranged by size: 2620 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4450 1537 Size: 275 2638 4090 Fragments arranged by size: 4090 2638 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2116 2383 2907 3554 4067 4933 1353 Size: 763 267 524 647 513 866 3423 Fragments arranged by size: 3423 866 763 647 524 513 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5590 5964 6110 387 Size: 1064 138 4001 374 146 1280 Fragments arranged by size: 4001 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2679 3246 3592 4014 5066 5663 Size: 393 629 418 567 346 422 1052 597 Cuts at: 5663 6450 6718 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1052 787 629 597 567 422 418 393 346 268 EcoO109I rG'GnC_Cy Cuts at: 2191 2985 3266 3822 2191 Size: 794 281 556 5372 Fragments arranged by size: 5372 794 556 281 EcoRI G'AATT_C Cuts at: 2601 4312 4690 2601 Size: 1711 378 4914 Fragments arranged by size: 4914 1711 378 FspI TGC'GCA Cuts at: 5509 5509 Size: 7003 HaeII r_GCGC'y Cuts at: 1678 2015 2091 2097 2103 6150 1678 Size: 337 76 6 6 4047 2531 Fragments arranged by size: 4047 2531 337 76 6 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2517 2549 2554 2559 2586 Size: 32 135 32 945 32 5 5 27 Cuts at: 2586 2591 3077 3109 3167 3199 4042 4074 5644 Size: 5 486 32 58 32 843 32 1570 Cuts at: 5644 5676 5718 5750 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1570 945 843 486 135 58 42 32 32 32 32 32 32 32 32 27 5 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2172 4347 4593 669 Size: 988 281 234 2175 246 3079 Fragments arranged by size: 3079 2175 988 281 246 234 HindIII A'AGCT_T Cuts at: 748 748 Size: 7003 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KasI G'GCGC_C Cuts at: 2087 2093 2099 2087 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 KpnI G_GTAC'C Cuts at: 2513 4328 2513 Size: 1815 5188 MfeI C'AATT_G Cuts at: 2625 4602 2625 Size: 1977 5026 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2305 2664 3067 6117 6301 1305 Size: 1000 359 403 3050 184 2007 Fragments arranged by size: 3050 2007 1000 403 359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5181 Fragments arranged by size: 5181 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 2839 3438 3881 4182 4961 5320 Size: 648 1266 407 599 443 301 779 359 Cuts at: 5320 5479 518 Size: 159 2042 Fragments arranged by size: 2042 1266 779 648 599 443 407 359 301 159 MspA1I CmG'CkG Cuts at: 1606 2105 2784 4419 5096 6244 6489 1606 Size: 499 679 1635 677 1148 245 2120 Fragments arranged by size: 2120 1635 1148 679 677 499 245 NaeI GCC'GGC Cuts at: 2086 2092 2098 2086 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 NarI GG'CG_CC Cuts at: 2088 2094 2100 2088 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5512 Fragments arranged by size: 5512 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NgoMIV G'CCGG_C Cuts at: 2084 2090 2096 2084 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NsiI A_TGCA'T Cuts at: 3928 3928 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 2675 2796 3536 4361 5906 1132 Size: 97 1446 121 740 825 1545 2229 Fragments arranged by size: 2229 1545 1446 825 740 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7003 PciI A'CATG_T Cuts at: 2792 3532 5902 2792 Size: 740 2370 3893 Fragments arranged by size: 3893 2370 740 PflMI CCAn_nnn'nTGG Cuts at: 1108 3858 1108 Size: 2750 4253 PfoI T'CCnGG_A Cuts at: 3262 3262 Size: 7003 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5072 5104 6585 6617 5072 Size: 32 1481 32 5458 Fragments arranged by size: 5458 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3266 3822 3266 Size: 556 6447 PshAI GACnn'nnGTC Cuts at: 1613 2754 1613 Size: 1141 5862 PsiI TTA'TAA Cuts at: 2802 4573 2802 Size: 1771 5232 PspOMI G'GGCC_C Cuts at: 2984 2984 Size: 7003 PspXI vC'TCGA_Gb Cuts at: 3340 3340 Size: 7003 PstI C_TGCA'G Cuts at: 830 4355 830 Size: 3525 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 SacII CC_GC'GG Cuts at: 2106 2106 Size: 7003 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SanDI GG'GwC_CC Cuts at: 3822 3822 Size: 7003 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 3516 5251 6963 3516 Size: 1735 1712 3556 Fragments arranged by size: 3556 1735 1712 SfcI C'TryA_G Cuts at: 826 1045 3798 4351 5486 6167 6358 826 Size: 219 2753 553 1135 681 191 1471 Fragments arranged by size: 2753 1471 1135 681 553 219 191 SfoI GGC'GCC Cuts at: 2089 2095 2101 2089 Size: 6 6 6991 Fragments arranged by size: 6991 6 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 2090 2096 1700 Size: 390 6 6607 Fragments arranged by size: 6607 390 6 SmaI CCC'GGG Cuts at: 1872 4330 1872 Size: 2458 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3340 3734 5107 6008 6270 820 Size: 197 926 1397 394 1373 901 262 1553 Fragments arranged by size: 1553 1397 1373 926 901 394 262 197 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7003 SphI G_CATG'C Cuts at: 1132 4361 1132 Size: 3229 3774 SspI AAT'ATT Cuts at: 5 52 3139 4927 5 Size: 47 3087 1788 2081 Fragments arranged by size: 3087 2081 1788 47 StuI AGG'CCT Cuts at: 1517 2246 1517 Size: 729 6274 StyI C'CwwG_G Cuts at: 513 1065 2004 3160 4442 513 Size: 552 939 1156 1282 3074 Fragments arranged by size: 3074 1282 1156 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2913 3102 4279 5030 5047 5200 5385 Size: 1255 1333 189 1177 751 17 153 185 Cuts at: 5385 5830 325 Size: 445 1498 Fragments arranged by size: 1498 1333 1255 1177 751 445 189 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2822 3514 5249 Size: 274 80 33 51 1443 845 692 1735 Cuts at: 5249 6961 96 Size: 1712 138 Fragments arranged by size: 1735 1712 1443 845 692 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2129 2161 3024 3056 6963 6995 2129 Size: 32 863 32 3907 32 2137 Fragments arranged by size: 3907 2137 863 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7003 XbaI T'CTAG_A Cuts at: 4339 4339 Size: 7003 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3167 3859 3167 Size: 692 6311 XhoI C'TCGA_G Cuts at: 1943 3340 1943 Size: 1397 5606 XmaI C'CCGG_G Cuts at: 1870 4328 1870 Size: 2458 4545 XmnI GAAnn'nnTTC Cuts at: 5132 5132 Size: 7003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI