Restriction map for pFN21AB5776
 (Linear) (Six-base) MAPSORT of: KIBB5776.seq  Check: 8149  from: 1  to: 2298



KIBB5776.orf

 With 182 enzymes: SgfI * 

                        July 22, 2009 17:02  ..

AccI GT'mk_AC

Cuts at:      0    1326    1412    2298 
   Size:       1326      86     886

  Fragments arranged by size: 

                1326     886      86
 
Acc65I G'GTAC_C

Cuts at:      0     504    2298 
   Size:        504    1794
 
AclI AA'CG_TT

Cuts at:      0     721    2298 
   Size:        721    1577
 
AflIII A'CryG_T

Cuts at:      0     787    1527    2298 
   Size:        787     740     771

  Fragments arranged by size: 

                 787     771     740
 
AleI CACnn'nnGTG

Cuts at:      0     427     834    2177    2298 
   Size:        427     407    1343     121

  Fragments arranged by size: 

                1343     427     407     121
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51     928    2298 
   Size:         51     877    1370

  Fragments arranged by size: 

                1370     877      51
 
ApaI G_GGCC'C

Cuts at:      0     983    2298 
   Size:        983    1315
 
ApoI r'AATT_y

Cuts at:      0     199     596     799     986    1639    2126    2168    2298 
   Size:        199     397     203     187     653     487      42     130

  Fragments arranged by size: 

                 653     487     397     203     199     187     130      42
            
 
AseI AT'TA_AT

Cuts at:      0    2074    2298 
   Size:       2074     224
 
AvaI C'yCGr_G

Cuts at:      0      73    1335    2298 
   Size:         73    1262     963

  Fragments arranged by size: 

                1262     963      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     488     521    2298 
   Size:        488      33    1777

  Fragments arranged by size: 

                1777     488      33
 
BamHI G'GATC_C

Cuts at:      0    1843    2298 
   Size:       1843     455
 
BanI G'GyrC_C

Cuts at:      0      82      88      94     504    1860    2298 
   Size:         82       6       6     410    1356     438

  Fragments arranged by size: 

                1356     438     410      82       6       6
 
BanII G_rGCy'C

Cuts at:      0      74     983    1603    2298 
   Size:         74     909     620     695

  Fragments arranged by size: 

                 909     695     620      74
 
BbeI G_GCGC'C

Cuts at:      0      86      92      98    2298 
   Size:         86       6       6    2200

  Fragments arranged by size: 

                2200      86       6       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     468    1304    1763    2298 
   Size:        468     836     459     535

  Fragments arranged by size: 

                 836     535     468     459
 
BbvCI CC'TCA_GC

Cuts at:      0    1265    1475    2298 
   Size:       1265     210     823

  Fragments arranged by size: 

                1265     823     210
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     947     981    2298 
   Size:        947      34    1317

  Fragments arranged by size: 

                1317     947      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     345    2008    2298 
   Size:        345    1663     290

  Fragments arranged by size: 

                1663     345     290
 
BclI T'GATC_A

Cuts at:      0    1649    2298 
   Size:       1649     649
 
BfrBI ATG'CAT

Cuts at:      0    1921    2298 
   Size:       1921     377
 
BglI GCCn_nnn'nGGC

Cuts at:      0     109    2298 
   Size:        109    2189
 
BglII A'GATC_T

Cuts at:      0    2028    2298 
   Size:       2028     270
 
BlpI GC'TnA_GC

Cuts at:      0      10    2298 
   Size:         10    2288
 
Bme1580I G_kGCm'C

Cuts at:      0     983    2298 
   Size:        983    1315
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1581    2298 
   Size:       1581     717
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     674    1241    1587    2009    2298 
   Size:        256     418     567     346     422     289

  Fragments arranged by size: 

                 567     422     418     346     289     256
 
Bpu10I CC'TnA_GC

Cuts at:      0     732     918    1265    1475    2298 
   Size:        732     186     347     210     823

  Fragments arranged by size: 

                 823     732     347     210     186
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1714    2298 
   Size:       1714     584
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1073    1145    1627    1718    2103    2298 
   Size:       1073      72     482      91     385     195

  Fragments arranged by size: 

                1073     482     385     195      91      72
 
BsaBI GATnn'nnATC

Cuts at:      0    1052    1089    1395    1889    2298 
   Size:       1052      37     306     494     409

  Fragments arranged by size: 

                1052     494     409     306      37
 
BsaHI Gr'CG_yC

Cuts at:      0      83      89      95     913    2298 
   Size:         83       6       6     818    1385

  Fragments arranged by size: 

                1385     818      83       6       6
 
BsaWI w'CCGG_w

Cuts at:      0     129    2298 
   Size:        129    2169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     124     154     512     542    2037    2067    2298 
   Size:        124      30     358      30    1495      30     231

  Fragments arranged by size: 

                1495     358     231     124      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148    2298 
   Size:        148    2150
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1664    2298 
   Size:       1664     634
 
BsmI GAATG_Cn'

Cuts at:      0    1883    2298 
   Size:       1883     415
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1007    2298 
   Size:       1007    1291
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     983    1603    2298 
   Size:         74     909     620     695

  Fragments arranged by size: 

                 909     695     620      74
 
BspEI T'CCGG_A

Cuts at:      0     129    2298 
   Size:        129    2169
 
BspHI T'CATG_A

Cuts at:      0    1752    2298 
   Size:       1752     546
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     516    2298 
   Size:        516    1782
 
BsrBI CCG'CTC

Cuts at:      0      14    2156    2298 
   Size:         14    2142     142

  Fragments arranged by size: 

                2142     142      14
 
BsrDI GCAATG_nn'

Cuts at:      0    2158    2298 
   Size:       2158     140
 
BsrFI r'CCGG_y

Cuts at:      0      79      85      91    2298 
   Size:         79       6       6    2207

  Fragments arranged by size: 

                2207      79       6       6
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     928    2298 
   Size:        928    1370
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     626    2298 
   Size:        429     197    1672

  Fragments arranged by size: 

                1672     429     197
 
BstYI r'GATC_y

Cuts at:      0    1390    1843    2028    2298 
   Size:       1390     453     185     270

  Fragments arranged by size: 

                1390     453     270     185
 
BtgI C'CryG_G

Cuts at:      0      98    2298 
   Size:         98    2200
 
BtsI GCAGTG_nn'

Cuts at:      0    1448    2184    2298 
   Size:       1448     736     114

  Fragments arranged by size: 

                1448     736     114
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     186     221    2298 
   Size:        186      35    2077

  Fragments arranged by size: 

                2077     186      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     111     378     902    1549    2062    2298 
   Size:        111     267     524     647     513     236

  Fragments arranged by size: 

                 647     524     513     267     236     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     674    1241    1587    2009    2298 
   Size:        256     418     567     346     422     289

  Fragments arranged by size: 

                 567     422     418     346     289     256
 
EcoO109I rG'GnC_Cy

Cuts at:      0     186     980    1261    1817    2298 
   Size:        186     794     281     556     481

  Fragments arranged by size: 

                 794     556     481     281     186
 
EcoRI G'AATT_C

Cuts at:      0     596    2298 
   Size:        596    1702
 
HaeII r_GCGC'y

Cuts at:      0      10      86      92      98    2298 
   Size:         10      76       6       6    2200

  Fragments arranged by size: 

                2200      76      10       6       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     512     544     549     554     581     586    1072    1104 
   Size:        512      32       5       5      27       5     486      32

Cuts at:   1104    1162    1194    2037    2069    2298 
   Size:         58      32     843      32     229

  Fragments arranged by size: 

                 843     512     486     229      58      32      32      32
                  32      27       5       5       5
 
HincII GTy'rAC

Cuts at:      0     167    2298 
   Size:        167    2131
 
KasI G'GCGC_C

Cuts at:      0      82      88      94    2298 
   Size:         82       6       6    2204

  Fragments arranged by size: 

                2204      82       6       6
 
KpnI G_GTAC'C

Cuts at:      0     508    2298 
   Size:        508    1790
 
MfeI C'AATT_G

Cuts at:      0     620    2298 
   Size:        620    1678
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     659    1062    2298 
   Size:        300     359     403    1236

  Fragments arranged by size: 

                1236     403     359     300
 
MslI CAynn'nnrTG

Cuts at:      0     427     834    1433    1876    2177    2298 
   Size:        427     407     599     443     301     121

  Fragments arranged by size: 

                 599     443     427     407     301     121
 
MspA1I CmG'CkG

Cuts at:      0     100     779    2298 
   Size:        100     679    1519

  Fragments arranged by size: 

                1519     679     100
 
NaeI GCC'GGC

Cuts at:      0      81      87      93    2298 
   Size:         81       6       6    2205

  Fragments arranged by size: 

                2205      81       6       6
 
NarI GG'CG_CC

Cuts at:      0      83      89      95    2298 
   Size:         83       6       6    2203

  Fragments arranged by size: 

                2203      83       6       6
 
NgoMIV G'CCGG_C

Cuts at:      0      79      85      91    2298 
   Size:         79       6       6    2207

  Fragments arranged by size: 

                2207      79       6       6
 
NsiI A_TGCA'T

Cuts at:      0    1923    2298 
   Size:       1923     375
 
NspI r_CATG'y

Cuts at:      0     670     791    1531    2298 
   Size:        670     121     740     767

  Fragments arranged by size: 

                 767     740     670     121
 
PciI A'CATG_T

Cuts at:      0     787    1527    2298 
   Size:        787     740     771

  Fragments arranged by size: 

                 787     771     740
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1853    2298 
   Size:       1853     445
 
PfoI T'CCnGG_A

Cuts at:      0    1257    2298 
   Size:       1257    1041
 
PpuMI rG'GwC_Cy

Cuts at:      0    1261    1817    2298 
   Size:       1261     556     481

  Fragments arranged by size: 

                1261     556     481
 
PshAI GACnn'nnGTC

Cuts at:      0     749    2298 
   Size:        749    1549
 
PsiI TTA'TAA

Cuts at:      0     797    2298 
   Size:        797    1501
 
PspOMI G'GGCC_C

Cuts at:      0     979    2298 
   Size:        979    1319
 
PspXI vC'TCGA_Gb

Cuts at:      0    1335    2298 
   Size:       1335     963
 
SacII CC_GC'GG

Cuts at:      0     101    2298 
   Size:        101    2197
 
SanDI GG'GwC_CC

Cuts at:      0    1817    2298 
   Size:       1817     481
 
ScaI AGT'ACT

Cuts at:      0    1511    2298 
   Size:       1511     787
 
SfcI C'TryA_G

Cuts at:      0    1793    2298 
   Size:       1793     505
 
SfoI GGC'GCC

Cuts at:      0      84      90      96    2298 
   Size:         84       6       6    2202

  Fragments arranged by size: 

                2202      84       6       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0      85      91    2298 
   Size:         85       6    2207

  Fragments arranged by size: 

                2207      85       6
 
SmlI C'TyrA_G

Cuts at:      0    1335    1729    2298 
   Size:       1335     394     569

  Fragments arranged by size: 

                1335     569     394
 
SspI AAT'ATT

Cuts at:      0    1134    2298 
   Size:       1134    1164
 
StuI AGG'CCT

Cuts at:      0     241    2298 
   Size:        241    2057
 
StyI C'CwwG_G

Cuts at:      0    1155    2298 
   Size:       1155    1143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     908    1097    2274    2298 
   Size:        908     189    1177      24

  Fragments arranged by size: 

                1177     908     189      24
 
TatI w'GTAC_w

Cuts at:      0     817    1509    2298 
   Size:        817     692     789

  Fragments arranged by size: 

                 817     789     692
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     124     156    1019    1051    2298 
   Size:        124      32     863      32    1247

  Fragments arranged by size: 

                1247     863     124      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1162    1854    2298 
   Size:       1162     692     444

  Fragments arranged by size: 

                1162     692     444
 
XhoI C'TCGA_G

Cuts at:      0    1335    2298 
   Size:       1335     963
 

 Enzymes that do cut:

     AccI   Acc65I     AclI   AflIII     AleI    AlwNI     ApaI     ApoI
     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
     BtgI     BtsI    CspCI     EarI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MmeI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI
    PpuMI    PshAI     PsiI   PspOMI    PspXI    SacII    SanDI     ScaI
     SfcI     SfoI    SgrAI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AcuI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI    AsiSI    AvrII    BmgBI     BmtI     BplI
    BsaAI    BseYI    BsiEI  BsiHKAI    BsiWI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     MluI     MscI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
     PpiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XmaI     XmnI     ZraI
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