(Circular) (Six-base) MAPSORT of: pFN21AE2239.seq Check: 5132 from: 1 to: 6004 pFN21AE2239 6004 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 2260 3325 2260 Size: 1065 4939 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 3141 4067 5451 5719 1632 Size: 561 948 926 1384 268 1917 Fragments arranged by size: 1917 1384 948 926 561 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2216 2288 2312 2324 3151 3747 5319 2216 Size: 72 24 12 827 596 1572 2901 Fragments arranged by size: 2901 1572 827 596 72 24 12 ApaLI G'TGCA_C Cuts at: 3014 4061 5217 3014 Size: 1047 1156 3801 Fragments arranged by size: 3801 1156 1047 ApoI r'AATT_y Cuts at: 1493 2648 3066 3259 3313 3539 3691 5800 1493 Size: 1155 418 193 54 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 418 226 193 152 54 AseI AT'TA_AT Cuts at: 160 1032 2037 4558 160 Size: 872 1005 2521 1606 Fragments arranged by size: 2521 1606 1005 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3329 1870 Size: 73 1386 4545 Fragments arranged by size: 4545 1386 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 2272 3325 4780 618 Size: 325 217 627 473 12 1053 1455 1842 Fragments arranged by size: 1842 1455 1053 627 473 325 217 12 BanII G_rGCy'C Cuts at: 1391 3323 1391 Size: 1932 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2430 928 Size: 971 531 4502 Fragments arranged by size: 4502 971 531 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2903 2937 4195 4229 1903 Size: 34 966 34 1258 34 3678 Fragments arranged by size: 3678 1258 966 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3055 3421 3055 Size: 366 5638 Bme1580I G_kGCm'C Cuts at: 1790 2413 2724 3018 4065 5221 1790 Size: 623 311 294 1047 1156 2573 Fragments arranged by size: 2573 1156 1047 623 311 294 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2591 3735 4693 Size: 416 53 83 399 24 1174 1144 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1174 1144 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2778 4664 1239 Size: 1539 1886 2579 Fragments arranged by size: 2579 1886 1539 Bpu10I CC'TnA_GC Cuts at: 2313 2325 2355 2313 Size: 12 30 5962 Fragments arranged by size: 5962 30 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 4129 4994 5292 2567 Size: 1562 865 298 3279 Fragments arranged by size: 3279 1562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2660 4667 882 Size: 617 1161 2007 2219 Fragments arranged by size: 2219 2007 1161 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2365 2395 2657 2687 1178 Size: 30 165 30 962 30 262 30 4495 Fragments arranged by size: 4495 962 262 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6004 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3249 1163 Size: 573 1513 3918 Fragments arranged by size: 3918 1513 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3018 4065 4150 5221 3018 Size: 1047 85 1071 3801 Fragments arranged by size: 3801 1071 1047 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2413 2724 3018 3323 4065 4150 5221 Size: 399 623 311 294 305 742 85 1071 Cuts at: 5221 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 623 399 311 305 294 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3359 844 Size: 2515 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 2673 96 Size: 2577 3427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 3292 3292 Size: 6004 BstYI r'GATC_y Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999 Size: 1362 367 385 17 1452 86 12 346 Cuts at: 5999 1972 Size: 1977 Fragments arranged by size: 1977 1452 1362 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2751 3520 4314 4334 2751 Size: 769 794 20 4421 Fragments arranged by size: 4421 794 769 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 2883 3308 2883 Size: 425 5579 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2970 3451 4340 Size: 54 1425 50 275 18 1140 481 889 Cuts at: 4340 5969 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1140 889 481 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2547 3135 3934 1353 Size: 1194 588 799 3423 Fragments arranged by size: 3423 1194 799 588 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2778 3141 4067 4664 5451 5719 Size: 393 561 585 363 926 597 787 268 Cuts at: 5719 1239 Size: 1524 Fragments arranged by size: 1524 926 787 597 585 561 393 363 268 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2432 Size: 6004 EcoRI G'AATT_C Cuts at: 3259 3313 3691 3259 Size: 54 378 5572 Fragments arranged by size: 5572 378 54 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2363 3146 2363 Size: 783 5221 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2012 5151 1678 Size: 334 3139 2531 Fragments arranged by size: 3139 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 3079 3111 3229 Size: 32 135 32 791 32 684 32 118 Cuts at: 3229 3261 4645 4677 4719 4751 1373 Size: 32 1384 32 42 32 2626 Fragments arranged by size: 2626 1384 791 684 135 118 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 2264 3329 2264 Size: 1065 4939 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 5118 5302 1305 Size: 1674 2139 184 2007 Fragments arranged by size: 2139 2007 1674 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2972 10 Size: 54 1425 343 1140 3042 Fragments arranged by size: 3042 1425 1140 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2404 2675 2756 3962 4321 4480 518 Size: 648 1238 271 81 1206 359 159 2042 Fragments arranged by size: 2042 1238 1206 648 359 271 159 81 MspA1I CmG'CkG Cuts at: 1606 2740 3256 3420 4097 5245 5490 1606 Size: 1134 516 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 1134 677 516 245 164 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3233 3362 4907 1132 Size: 97 2004 129 1545 2229 Fragments arranged by size: 2229 2004 1545 129 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2801 1108 Size: 1693 4311 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2430 1613 Size: 817 5187 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 2175 2419 2739 3356 830 Size: 1345 244 320 617 3478 Fragments arranged by size: 3478 1345 617 320 244 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2740 3256 2740 Size: 516 5488 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SapI GCTCTTCn'nnn_ Cuts at: 2547 2547 Size: 6004 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 2171 2415 2735 3352 4487 5168 5359 Size: 219 1126 244 320 617 1135 681 191 Cuts at: 5359 826 Size: 1471 Fragments arranged by size: 1471 1135 1126 681 617 320 244 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2546 4108 5009 5271 820 Size: 197 926 603 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 901 603 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 3268 152 Size: 3116 2888 SphI G_CATG'C Cuts at: 1132 3233 3362 1132 Size: 2101 129 3774 Fragments arranged by size: 3774 2101 129 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2004 3285 3443 513 Size: 552 939 1281 158 3074 Fragments arranged by size: 3074 1281 939 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2632 2673 4250 Size: 274 80 33 51 1443 655 41 1577 Cuts at: 4250 5962 96 Size: 1712 138 Fragments arranged by size: 1712 1577 1443 655 274 138 80 51 41 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 3129 4133 3129 Size: 1004 5000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI