(Linear) (Six-base) MAPSORT of: KIEE1535.seq Check: 1279 from: 1 to: 2331 KIEE1535.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 307 1246 1761 2331 Size: 295 12 939 515 570 Fragments arranged by size: 939 570 515 295 12 AflIII A'CryG_T Cuts at: 0 174 1904 2331 Size: 174 1730 427 Fragments arranged by size: 1730 427 174 AleI CACnn'nnGTG Cuts at: 0 1108 1255 2331 Size: 1108 147 1076 Fragments arranged by size: 1108 1076 147 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2008 2040 2331 Size: 2008 32 291 Fragments arranged by size: 2008 291 32 AlwNI CAG_nnn'CTG Cuts at: 0 57 1224 1359 1539 1605 2331 Size: 57 1167 135 180 66 726 Fragments arranged by size: 1167 726 180 135 66 57 ApoI r'AATT_y Cuts at: 0 138 430 2331 Size: 138 292 1901 Fragments arranged by size: 1901 292 138 AvaI C'yCGr_G Cuts at: 0 703 1439 1749 2331 Size: 703 736 310 582 Fragments arranged by size: 736 703 582 310 BanI G'GyrC_C Cuts at: 0 16 945 2226 2331 Size: 16 929 1281 105 Fragments arranged by size: 1281 929 105 16 BanII G_rGCy'C Cuts at: 0 368 471 641 881 977 1036 1804 2331 Size: 368 103 170 240 96 59 768 527 Fragments arranged by size: 768 527 368 240 170 103 96 59 BbeI G_GCGC'C Cuts at: 0 949 2331 Size: 949 1382 BbvCI CC'TCA_GC Cuts at: 0 488 897 956 2331 Size: 488 409 59 1375 Fragments arranged by size: 1375 488 409 59 BglI GCCn_nnn'nGGC Cuts at: 0 1444 1501 1787 1927 1945 2029 2331 Size: 1444 57 286 140 18 84 302 Fragments arranged by size: 1444 302 286 140 84 57 18 BglII A'GATC_T Cuts at: 0 89 2331 Size: 89 2242 Bme1580I G_kGCm'C Cuts at: 0 419 1058 1094 1458 2331 Size: 419 639 36 364 873 Fragments arranged by size: 873 639 419 364 36 BmgBI CAC'GTC Cuts at: 0 177 420 2331 Size: 177 243 1911 Fragments arranged by size: 1911 243 177 BmrI ACTGGGnnnn_n' Cuts at: 0 486 1011 1934 2331 Size: 486 525 923 397 Fragments arranged by size: 923 525 486 397 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1155 1753 2331 Size: 1155 598 578 Fragments arranged by size: 1155 598 578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 1381 1561 1594 1660 1693 2331 Size: 214 1167 180 33 66 33 638 Fragments arranged by size: 1167 638 214 180 66 33 33 Bpu10I CC'TnA_GC Cuts at: 0 488 897 956 971 2331 Size: 488 409 59 15 1360 Fragments arranged by size: 1360 488 409 59 15 BsaAI yAC'GTr Cuts at: 0 854 2331 Size: 854 1477 BsaHI Gr'CG_yC Cuts at: 0 946 2331 Size: 946 1385 BsaWI w'CCGG_w Cuts at: 0 356 992 2331 Size: 356 636 1339 Fragments arranged by size: 1339 636 356 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2008 2027 2038 2057 2331 Size: 2008 19 11 19 274 Fragments arranged by size: 2008 274 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 283 790 793 796 838 885 1033 1219 Size: 283 507 3 3 42 47 148 186 Cuts at: 1219 1513 2331 Size: 294 818 Fragments arranged by size: 818 507 294 283 186 148 47 42 3 3 BseYI C'CCAG_C Cuts at: 0 737 1703 1768 1858 1867 1885 1911 2180 Size: 737 966 65 90 9 18 26 269 Cuts at: 2180 2246 2267 2312 2331 Size: 66 21 45 19 Fragments arranged by size: 966 737 269 90 66 65 45 26 21 19 18 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1058 1600 2331 Size: 532 526 542 731 Fragments arranged by size: 731 542 532 526 BsiEI CG_ry'CG Cuts at: 0 1313 2331 Size: 1313 1018 BsiHKAI G_wGCw'C Cuts at: 0 977 1319 1472 1739 2331 Size: 977 342 153 267 592 Fragments arranged by size: 977 592 342 267 153 BsmI GAATG_Cn' Cuts at: 0 499 2331 Size: 499 1832 BsmBI CGTCTCn'nnnn_ Cuts at: 0 34 754 2174 2331 Size: 34 720 1420 157 Fragments arranged by size: 1420 720 157 34 Bsp1286I G_dGCh'C Cuts at: 0 368 419 471 641 881 977 1036 1058 Size: 368 51 52 170 240 96 59 22 Cuts at: 1058 1094 1319 1458 1472 1739 1804 2331 Size: 36 225 139 14 267 65 527 Fragments arranged by size: 527 368 267 240 225 170 139 96 65 59 52 51 36 22 14 BspEI T'CCGG_A Cuts at: 0 356 2331 Size: 356 1975 BspHI T'CATG_A Cuts at: 0 237 401 2141 2331 Size: 237 164 1740 190 Fragments arranged by size: 1740 237 190 164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 290 2331 Size: 290 2041 BsrBI CCG'CTC Cuts at: 0 1626 2331 Size: 1626 705 BsrFI r'CCGG_y Cuts at: 0 1068 1786 1833 2292 2331 Size: 1068 718 47 459 39 Fragments arranged by size: 1068 718 459 47 39 BsrGI T'GTAC_A Cuts at: 0 1820 2331 Size: 1820 511 BssHII G'CGCG_C Cuts at: 0 1668 1670 2331 Size: 1668 2 661 Fragments arranged by size: 1668 661 2 BssSI C'ACGA_G Cuts at: 0 1213 2331 Size: 1213 1118 BstAPI GCAn_nnn'nTGC Cuts at: 0 130 2331 Size: 130 2201 BstXI CCAn_nnnn'nTGG Cuts at: 0 1110 1113 2079 2331 Size: 1110 3 966 252 Fragments arranged by size: 1110 966 252 3 BstYI r'GATC_y Cuts at: 0 89 320 1427 1520 2331 Size: 89 231 1107 93 811 Fragments arranged by size: 1107 811 231 93 89 BtgI C'CryG_G Cuts at: 0 263 2078 2108 2261 2331 Size: 263 1815 30 153 70 Fragments arranged by size: 1815 263 153 70 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 458 1448 2178 2331 Size: 458 990 730 153 Fragments arranged by size: 990 730 458 153 BtsI GCAGTG_nn' Cuts at: 0 540 1871 2331 Size: 540 1331 460 Fragments arranged by size: 1331 540 460 DraIII CAC_nnn'GTG Cuts at: 0 1818 2331 Size: 1818 513 EaeI y'GGCC_r Cuts at: 0 225 248 1310 1592 2048 2331 Size: 225 23 1062 282 456 283 Fragments arranged by size: 1062 456 283 282 225 23 EagI C'GGCC_G Cuts at: 0 1310 2331 Size: 1310 1021 EarI CTCTTCn'nnn_ Cuts at: 0 283 930 1264 1330 1460 2331 Size: 283 647 334 66 130 871 Fragments arranged by size: 871 647 334 283 130 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 94 835 889 2331 Size: 94 741 54 1442 Fragments arranged by size: 1442 741 94 54 EcoICRI GAG'CTC Cuts at: 0 975 2331 Size: 975 1356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 295 307 1246 1381 1561 1594 1660 Size: 214 81 12 939 135 180 33 66 Cuts at: 1660 1693 1761 2331 Size: 33 68 570 Fragments arranged by size: 939 570 214 180 135 81 68 66 33 33 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 37 334 772 1085 2331 Size: 37 297 438 313 1246 Fragments arranged by size: 1246 438 313 297 37 EcoO109I rG'GnC_Cy Cuts at: 0 279 2031 2331 Size: 279 1752 300 Fragments arranged by size: 1752 300 279 EcoRI G'AATT_C Cuts at: 0 138 430 2331 Size: 138 292 1901 Fragments arranged by size: 1901 292 138 HaeII r_GCGC'y Cuts at: 0 805 904 949 1234 1450 1512 1518 1605 Size: 805 99 45 285 216 62 6 87 Cuts at: 1605 2331 Size: 726 Fragments arranged by size: 805 726 285 216 99 87 62 45 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 61 93 1125 1157 2331 Size: 61 32 1032 32 1174 Fragments arranged by size: 1174 1032 61 32 32 HincII GTy'rAC Cuts at: 0 159 2331 Size: 159 2172 HindIII A'AGCT_T Cuts at: 0 1480 2331 Size: 1480 851 KasI G'GCGC_C Cuts at: 0 945 2331 Size: 945 1386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 41 2331 Size: 41 2290 MscI TGG'CCA Cuts at: 0 227 250 1594 2331 Size: 227 23 1344 737 Fragments arranged by size: 1344 737 227 23 MslI CAynn'nnrTG Cuts at: 0 406 1108 1255 2331 Size: 406 702 147 1076 Fragments arranged by size: 1076 702 406 147 MspA1I CmG'CkG Cuts at: 0 14 503 654 741 792 1356 1377 1527 Size: 14 489 151 87 51 564 21 150 Cuts at: 1527 1590 1846 2005 2331 Size: 63 256 159 326 Fragments arranged by size: 564 489 326 256 159 151 150 87 63 51 21 14 NaeI GCC'GGC Cuts at: 0 1070 1788 1835 2294 2331 Size: 1070 718 47 459 37 Fragments arranged by size: 1070 718 459 47 37 NarI GG'CG_CC Cuts at: 0 946 2331 Size: 946 1385 NcoI C'CATG_G Cuts at: 0 263 2078 2108 2331 Size: 263 1815 30 223 Fragments arranged by size: 1815 263 223 30 NgoMIV G'CCGG_C Cuts at: 0 1068 1786 1833 2292 2331 Size: 1068 718 47 459 39 Fragments arranged by size: 1068 718 459 47 39 NspI r_CATG'y Cuts at: 0 352 1284 1347 1866 1908 2133 2331 Size: 352 932 63 519 42 225 198 Fragments arranged by size: 932 519 352 225 198 63 42 PciI A'CATG_T Cuts at: 0 1904 2331 Size: 1904 427 PflMI CCAn_nnn'nTGG Cuts at: 0 1539 2019 2223 2331 Size: 1539 480 204 108 Fragments arranged by size: 1539 480 204 108 PfoI T'CCnGG_A Cuts at: 0 383 1127 2331 Size: 383 744 1204 Fragments arranged by size: 1204 744 383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1168 1200 2008 2040 2331 Size: 1168 32 808 32 291 Fragments arranged by size: 1168 808 291 32 32 PpuMI rG'GwC_Cy Cuts at: 0 279 2331 Size: 279 2052 PshAI GACnn'nnGTC Cuts at: 0 846 2331 Size: 846 1485 PstI C_TGCA'G Cuts at: 0 508 773 791 1238 1412 1610 2331 Size: 508 265 18 447 174 198 721 Fragments arranged by size: 721 508 447 265 198 174 18 PvuII CAG'CTG Cuts at: 0 654 741 792 1356 1377 1527 1590 2331 Size: 654 87 51 564 21 150 63 741 Fragments arranged by size: 741 654 564 150 87 63 51 21 SacI G_AGCT'C Cuts at: 0 977 2331 Size: 977 1354 SapI GCTCTTCn'nnn_ Cuts at: 0 1460 2331 Size: 1460 871 SbfI CC_TGCA'GG Cuts at: 0 773 1610 2331 Size: 773 837 721 Fragments arranged by size: 837 773 721 ScaI AGT'ACT Cuts at: 0 589 2331 Size: 589 1742 SfcI C'TryA_G Cuts at: 0 504 769 787 1234 1408 1606 2331 Size: 504 265 18 447 174 198 725 Fragments arranged by size: 725 504 447 265 198 174 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1945 2029 2331 Size: 1945 84 302 Fragments arranged by size: 1945 302 84 SfoI GGC'GCC Cuts at: 0 947 2331 Size: 947 1384 SmaI CCC'GGG Cuts at: 0 1441 2331 Size: 1441 890 SphI G_CATG'C Cuts at: 0 352 1347 1866 2331 Size: 352 995 519 465 Fragments arranged by size: 995 519 465 352 StuI AGG'CCT Cuts at: 0 340 2331 Size: 340 1991 StyI C'CwwG_G Cuts at: 0 108 263 869 2078 2108 2331 Size: 108 155 606 1209 30 223 Fragments arranged by size: 1209 606 223 155 108 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 468 2331 Size: 468 1863 TatI w'GTAC_w Cuts at: 0 371 587 920 1394 1820 2331 Size: 371 216 333 474 426 511 Fragments arranged by size: 511 474 426 371 333 216 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2027 2059 2331 Size: 2027 32 272 Fragments arranged by size: 2027 272 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 375 1110 2085 2331 Size: 375 735 975 246 Fragments arranged by size: 975 735 375 246 XmaI C'CCGG_G Cuts at: 0 1439 2331 Size: 1439 892 XmnI GAAnn'nnTTC Cuts at: 0 576 2331 Size: 576 1755 Enzymes that do cut: AcuI AflIII AleI AloI AlwNI ApoI AvaI BanI BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI PpuMI PshAI PstI PvuII SacI SapI SbfI ScaI SfcI SfiI SfoI SmaI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI BmtI BpuEI BsaI BsaBI BsiWI BsrDI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI