Restriction map for
pFN21AE1086
##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AhdI Aor51HI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BglI BlnI BlpI Bpu1102I BsgI
BsiWI BspDI BssHII Bst1107I BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
KasI KspI MluI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshAI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 6087 CGCG CG^CG
AccIII 5708 TCCGGA T^CCGGA
AclI 2938 AACGTT AA^CGTT
AcyI 1292 GGCGTC G[GA]^CG[CT]C
AgeI 6684 ACCGGT A^CCGGT
Alw44I 1391 GTGCAC G^TGCAC
AocI 5971 CCTGAGG CC^T[ACGT]AGG
ApaLI 1391 GTGCAC G^TGCAC
AseI 7033 ATTAAT AT^TAAT
AsnI 7033 ATTAAT AT^TAAT
AspI 2101 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3327 CCCGAG C^[CT]CG[GA]G
BalI 511 TGGCCA TGG^CCA
BcgI 314 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2001 CCGGG CC^[GC]GG
BglII 6220 AGATCT A^GATCT
BsaHI 1292 GGCGTC G[GA]^CG[CT]C
BseAI 5708 TCCGGA T^CCGGA
BsiEI 6330 CGATCG CG[GA][CT]^CG
BsoBI 3327 CCCGAG C^[CT]CG[GA]G
BspEI 5708 TCCGGA T^CCGGA
BsrBI 6324 CCGCTC CCGCTC(-3/-3)
BsrFI 6684 ACCGGT [GA]^CCGG[CT]
BssSI 4955 CACGAG CACGAG(-5/-1)
BstUI 6087 CGCG CG^CG
BstXI 3374 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5971 CCTGAGG CC^T[ACGT]AGG
CfoI 7723 GCGC GCG^C
Cfr10I 6684 ACCGGT [GA]^CCGG[CT]
DrdI 6537 GACAGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5971 CCTGAGG CC^T[ACGT]AGG
EcoNI 6993 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5047 ATGCAT ATGCA^T
HaeII 7722 AGCGCC [GA]GCGC^[CT]
HgaI 1293 GCGTC GACGC(5/10)
HhaI 7723 GCGC GCG^C
Hin1I 1292 GGCGTC G[GA]^CG[CT]C
HinP1I 7723 GCGC G^CGC
HpaI 6270 GTTAAC GTT^AAC
MluNI 511 TGGCCA TGG^CCA
MroI 5708 TCCGGA T^CCGGA
MscI 511 TGGCCA TGG^CCA
MvnI 6087 CGCG CG^CG
NciI 2001 CCGGG CC^[GC]GG
NsiI 5047 ATGCAT ATGCA^T
PflFI 2101 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6853 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 6684 ACCGGT A^CCGGT
PshBI 7033 ATTAAT AT^TAAT
Psp1406I 2938 AACGTT AA^CGTT
PvuI 6330 CGATCG CGAT^CG
SphI 5277 GCATGC GCATG^C
SwaI 950 ATTTAAAT ATTT^AAAT
Tth111I 2101 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6853 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 2652 GGTACC G^GTACC
6823 GGTACC
Asp718I 2652 GGTACC G^GTACC
6823 GGTACC
BanI 2652 GGTACC G^G[CT][GA]CC
6823 GGTACC
BbvCI 1257 CCTCAGC CCTCAGC(-5/-2)
5142 GCTGAGG
BclI 3768 TGATCA T^GATCA
7632 TGATCA
BsaAI 5313 CACGTA [CT]AC^GT[GA]
5866 TACGTG
BsaBI 2956 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3313 GATCCATATC
BseRI 1225 GAGGAG GAGGAG(10/8)
4329 GAGGAG
BsmBI 1297 CGTCTC CGTCTC(1/5)
1307 CGTCTC
Bsp1407I 87 TGTACA T^GTACA
6336 TGTACA
BspMI 4534 ACCTGC ACCTGC(4/8)
4483 GCAGGT
BsrGI 87 TGTACA T^GTACA
6336 TGTACA
BstAPI 3016 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3025 GCAGCTTCTGC
BstEII 4315 GGTCACC G^GT[ACGT]ACC
5803 GGTCACC
BstPI 4315 GGTCACC G^GT[ACGT]ACC
5803 GGTCACC
DraIII 1901 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
4764 CACACAGTG
EcoO65I 4315 GGTCACC G^GT[ACGT]ACC
5803 GGTCACC
EcoRV 3334 GATATC GAT^ATC
5443 GATATC
FauI 37 CCCGC CCCGC(4/6)
45 GCGGG
FbaI 3768 TGATCA T^GATCA
7632 TGATCA
KpnI 2652 GGTACC GGTAC^C
6823 GGTACC
MamI 2956 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3313 GATCCATATC
NheI 796 GCTAGC G^CTAGC
6841 GCTAGC
PpuMI 3486 GGGACCC [GA]G^G[AT]CC[CT]
7569 GGGTCCT
SapI 874 GAAGAGC GCTCTTC(1/4)
7598 GAAGAGC
ScaI 1169 AGTACT AGT^ACT
6131 AGTACT
SpeI 5472 ACTAGT A^CTAGT
6012 ACTAGT
SspBI 87 TGTACA T^GTACA
6336 TGTACA
XbaI 4896 TCTAGA T^CTAGA
6666 TCTAGA
XcmI 1685 CCATTGATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7902 CCAGATGTATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApaI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BciVI BfaI BfrI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BsrDI BsrI BssKI BstBI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NspI NspV PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI