(Circular) (Six-base) MAPSORT of: pFN21AE1086.seq Check: 9954 from: 1 to: 12637 pFN21AE1086 12637 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6548 6548 Size: 12637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10447 278 Size: 53 83 186 936 8911 2468 Fragments arranged by size: 8911 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6846 7253 9013 9980 1937 Size: 4909 407 1760 967 4594 Fragments arranged by size: 4909 4594 1760 967 407 Acc65I G'GTAC_C Cuts at: 4657 8828 9958 4657 Size: 4171 1130 7336 Fragments arranged by size: 7336 4171 1130 AclI AA'CG_TT Cuts at: 1563 4944 10764 11137 1563 Size: 3381 5820 373 3063 Fragments arranged by size: 5820 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3407 4536 4619 5192 5831 5843 8906 10700 Size: 1775 1129 83 573 639 12 3063 1794 Cuts at: 10700 12084 12352 1632 Size: 1384 268 1917 Fragments arranged by size: 3063 1917 1794 1775 1384 1129 639 573 268 83 12 AflII C'TTAA_G Cuts at: 820 1017 3014 3635 4546 6442 9049 820 Size: 197 1997 621 911 1896 2607 4408 Fragments arranged by size: 4408 2607 1997 1896 911 621 197 AflIII A'CryG_T Cuts at: 1203 3361 4213 5500 7317 7569 7989 9478 9859 Size: 2158 852 1287 1817 252 420 1489 381 Cuts at: 9859 11536 12513 1203 Size: 1677 977 1327 Fragments arranged by size: 2158 1817 1677 1489 1327 1287 977 852 420 381 252 AgeI A'CCGG_T Cuts at: 8689 11468 8689 Size: 2779 9858 AhdI GACnn_n'nnGTC Cuts at: 11366 11366 Size: 12637 AleI CACnn'nnGTG Cuts at: 1166 3629 7268 1166 Size: 2463 3639 6535 Fragments arranged by size: 6535 3639 2463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3058 1184 Size: 1874 10763 AlwNI CAG_nnn'CTG Cuts at: 3673 4709 5036 5419 6799 10380 11952 3673 Size: 1036 327 383 1380 3581 1572 4358 Fragments arranged by size: 4358 3581 1572 1380 1036 383 327 ApaI G_GGCC'C Cuts at: 3188 3982 5333 3188 Size: 794 1351 10492 Fragments arranged by size: 10492 1351 794 ApaLI G'TGCA_C Cuts at: 3396 10694 11850 3396 Size: 7298 1156 4183 Fragments arranged by size: 7298 4183 1156 ApoI r'AATT_y Cuts at: 1493 2109 2297 2605 2612 3068 3708 4051 4121 Size: 616 188 308 7 456 640 343 70 Cuts at: 4121 4435 6531 6788 7678 8448 8907 9023 9281 Size: 314 2096 257 890 770 459 116 258 Cuts at: 9281 9946 10172 10324 12433 1493 Size: 665 226 152 2109 1697 Fragments arranged by size: 2109 2096 1697 890 770 665 640 616 459 456 343 314 308 258 257 226 188 152 116 70 7 AseI AT'TA_AT Cuts at: 160 1032 9039 11191 160 Size: 872 8007 2152 1606 Fragments arranged by size: 8007 2152 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12637 AvaI C'yCGr_G Cuts at: 1870 1943 5332 9962 1870 Size: 73 3389 4630 4545 Fragments arranged by size: 4630 4545 3389 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6379 6412 9346 9379 6379 Size: 33 2934 33 9637 Fragments arranged by size: 9637 2934 33 33 BamHI G'GATC_C Cuts at: 9967 10334 9967 Size: 367 12270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4657 8828 9958 11413 618 Size: 325 217 627 2870 4171 1130 1455 1842 Fragments arranged by size: 4171 2870 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 3188 3982 3992 4744 5333 5426 5633 6244 Size: 1797 794 10 752 589 93 207 611 Cuts at: 6244 9956 1391 Size: 3712 4072 Fragments arranged by size: 4072 3712 1797 794 752 611 589 207 93 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 3000 6882 928 Size: 971 573 528 3882 6683 Fragments arranged by size: 6683 3882 971 573 528 BbvCI CC'TCA_GC Cuts at: 3263 7148 3263 Size: 3885 8752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2308 2342 10828 10862 1903 Size: 34 371 34 8486 34 3678 Fragments arranged by size: 8486 3678 371 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2127 2848 4597 6913 10529 11739 2127 Size: 721 1749 2316 3616 1210 3025 Fragments arranged by size: 3616 3025 2316 1749 1210 721 BclI T'GATC_A Cuts at: 1548 5773 9637 1548 Size: 4225 3864 4548 Fragments arranged by size: 4548 4225 3864 BfrBI ATG'CAT Cuts at: 7054 7054 Size: 12637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11248 136 Size: 107 122 71 631 605 9576 1525 Fragments arranged by size: 9576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8225 12632 8225 Size: 4407 8230 BlpI GC'TnA_GC Cuts at: 10054 10054 Size: 12637 Bme1580I G_kGCm'C Cuts at: 1790 3188 3400 3982 5161 5333 10698 11854 1790 Size: 1398 212 582 1179 172 5365 1156 2573 Fragments arranged by size: 5365 2573 1398 1179 1156 582 212 172 BmgBI CAC'GTC Cuts at: 1318 4879 1318 Size: 3561 9076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3985 5204 6626 Size: 416 53 83 399 24 2568 1219 1422 Cuts at: 6626 7424 7660 10368 11326 442 Size: 798 236 2708 958 1753 Fragments arranged by size: 2708 2568 1753 1422 1219 958 798 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2805 8850 1056 Size: 1749 6045 4843 Fragments arranged by size: 6045 4843 1749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9302 9302 Size: 12637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 3701 4737 5792 6180 6441 7602 9765 Size: 803 1659 1036 1055 388 261 1161 2163 Cuts at: 9765 11297 1239 Size: 1532 2579 Fragments arranged by size: 2579 2163 1659 1532 1161 1055 1036 803 388 261 Bpu10I CC'TnA_GC Cuts at: 3263 6345 7148 3263 Size: 3082 803 8752 Fragments arranged by size: 8752 3082 803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3971 6033 6117 6228 7617 10762 11627 11925 3971 Size: 2062 84 111 1389 3145 865 298 4683 Fragments arranged by size: 4683 3145 2062 1389 865 298 111 84 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5819 6299 9157 9420 11300 882 Size: 617 4320 480 2858 263 1880 2219 Fragments arranged by size: 4320 2858 2219 1880 617 480 263 BsaAI yAC'GTr Cuts at: 493 1204 7320 7873 10428 493 Size: 711 6116 553 2555 2702 Fragments arranged by size: 6116 2702 2555 711 553 BsaBI GATnn'nnATC Cuts at: 4965 5322 12631 4965 Size: 357 7309 4971 Fragments arranged by size: 7309 4971 357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3298 10444 10826 11461 Size: 53 83 186 936 1765 7146 382 635 Cuts at: 11461 275 Size: 1451 Fragments arranged by size: 7146 1765 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2130 7713 8199 8689 11070 11468 11742 11889 12300 Size: 5583 486 490 2381 398 274 147 411 Cuts at: 12300 2130 Size: 2467 Fragments arranged by size: 5583 2467 2381 490 486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4641 4671 9738 9768 1178 Size: 30 165 30 3238 30 5067 30 4047 Fragments arranged by size: 5067 4047 3238 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3245 6349 1186 Size: 2059 3104 7474 Fragments arranged by size: 7474 3104 2059 BseYI C'CCAG_C Cuts at: 2056 3080 11840 12336 2056 Size: 1024 8760 496 2357 Fragments arranged by size: 8760 2357 1024 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 12064 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8338 10087 10848 11876 1540 Size: 275 186 6337 1749 761 1028 2301 Fragments arranged by size: 6337 2301 1749 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3006 3337 3400 3992 4744 5426 5840 6244 8726 Size: 331 63 592 752 682 414 404 2482 Cuts at: 8726 10698 10783 11854 3006 Size: 1972 85 1071 3789 Fragments arranged by size: 3789 2482 1972 1071 752 682 592 414 404 331 85 63 BsmI GAATG_Cn' Cuts at: 3786 4876 7612 7775 9476 9718 10148 10241 3786 Size: 1090 2736 163 1701 242 430 93 6182 Fragments arranged by size: 6182 2736 1701 1090 430 242 163 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3308 3318 3308 Size: 10 12627 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3006 3188 3337 3400 3982 3992 4744 Size: 399 1216 182 149 63 582 10 752 Cuts at: 4744 5161 5333 5426 5633 5840 6244 8726 9956 Size: 417 172 93 207 207 404 2482 1230 Cuts at: 9956 10698 10783 11854 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2482 2174 1230 1216 1071 752 742 582 417 404 399 207 207 182 172 149 93 85 63 10 BspEI T'CCGG_A Cuts at: 7713 12300 7713 Size: 4587 8050 BspHI T'CATG_A Cuts at: 2229 2526 5752 8799 8973 9313 10526 12256 2229 Size: 297 3226 3047 174 340 1213 1730 2610 Fragments arranged by size: 3226 3047 2610 1730 1213 340 297 174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6479 6548 9992 844 Size: 5635 69 3444 3489 Fragments arranged by size: 5635 3489 3444 69 BsrBI CCG'CTC Cuts at: 1394 1823 8331 10524 1394 Size: 429 6508 2193 3507 Fragments arranged by size: 6508 3507 2193 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6708 8065 8535 11132 11306 65 Size: 1183 5460 1357 470 2597 174 1396 Fragments arranged by size: 5460 2597 1396 1357 1183 470 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8689 11281 11468 1700 Size: 110 6879 2592 187 2869 Fragments arranged by size: 6879 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 2092 8341 96 Size: 1996 6249 4392 Fragments arranged by size: 6249 4392 1996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12637 BssSI C'ACGA_G Cuts at: 6960 10697 11709 6960 Size: 3737 1012 7888 Fragments arranged by size: 7888 3737 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5027 5036 794 Size: 4233 9 8395 Fragments arranged by size: 8395 4233 9 BstBI TT'CG_AA Cuts at: 5047 9091 9601 11441 5047 Size: 4044 510 1840 6243 Fragments arranged by size: 6243 4044 1840 510 BstEII G'GTnAC_C Cuts at: 1184 6320 7808 1184 Size: 5136 1488 6013 Fragments arranged by size: 6013 5136 1488 BstXI CCAn_nnnn'nTGG Cuts at: 5386 5386 Size: 12637 BstYI r'GATC_y Cuts at: 1972 4447 6097 7119 8225 9967 10334 10719 10736 Size: 2475 1650 1022 1106 1742 367 385 17 Cuts at: 10736 12188 12274 12286 12632 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2475 1977 1742 1650 1452 1106 1022 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 7977 7977 Size: 12637 BtgI C'CryG_G Cuts at: 513 1065 2004 3794 7213 7999 8157 513 Size: 552 939 1790 3419 786 158 4993 Fragments arranged by size: 4993 3419 1790 939 786 552 158 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 11898 Fragments arranged by size: 11898 683 56 BtsI GCAGTG_nn' Cuts at: 2068 7158 9150 9384 10153 10947 10967 2068 Size: 5090 1992 234 769 794 20 3738 Fragments arranged by size: 5090 3738 1992 794 769 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8513 8548 529 Size: 35 7949 35 4618 Fragments arranged by size: 7949 4618 35 35 DraI TTT'AAA Cuts at: 2407 2958 3828 4920 8734 8946 9941 2407 Size: 551 870 1092 3814 212 995 5103 Fragments arranged by size: 5103 3814 1092 995 870 551 212 DraIII CAC_nnn'GTG Cuts at: 3911 6774 3911 Size: 2863 9774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8548 11644 809 Size: 507 7232 3096 1802 Fragments arranged by size: 7232 3096 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2516 7071 8326 Size: 54 1425 50 275 18 686 4555 1255 Cuts at: 8326 10084 10973 12602 8 Size: 1758 889 1629 43 Fragments arranged by size: 4555 1758 1629 1425 1255 889 686 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10084 1537 Size: 275 8272 4090 Fragments arranged by size: 8272 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2874 4161 4229 5862 8925 9070 9598 10567 Size: 1521 1287 68 1633 3063 145 528 969 Cuts at: 10567 1353 Size: 3423 Fragments arranged by size: 3423 3063 1633 1521 1287 969 528 145 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11224 11598 11744 387 Size: 1064 138 9635 374 146 1280 Fragments arranged by size: 9635 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3990 4742 5424 6242 3990 Size: 752 682 818 10385 Fragments arranged by size: 10385 818 752 682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 3407 3701 4536 4619 4737 5192 Size: 393 410 1365 294 835 83 118 455 Cuts at: 5192 5792 5831 5843 6180 6441 7602 8906 9765 Size: 600 39 12 337 261 1161 1304 859 Cuts at: 9765 10700 11297 12084 12352 1239 Size: 935 597 787 268 1524 Fragments arranged by size: 1524 1365 1304 1161 935 859 835 787 600 597 455 410 393 337 294 268 261 118 83 39 12 EcoNI CCTnn'n_nnAGG Cuts at: 9002 9002 Size: 12637 EcoO109I rG'GnC_Cy Cuts at: 3979 5329 5492 9575 3979 Size: 1350 163 4083 7041 Fragments arranged by size: 7041 4083 1350 163 EcoRI G'AATT_C Cuts at: 2297 4051 7678 9023 9946 10324 2297 Size: 1754 3627 1345 923 378 4610 Fragments arranged by size: 4610 3627 1754 1345 923 378 EcoRV GAT'ATC Cuts at: 5341 7450 5341 Size: 2109 10528 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4436 4624 5765 5950 4436 Size: 188 1141 185 11123 Fragments arranged by size: 11123 1141 188 185 FspI TGC'GCA Cuts at: 11143 11143 Size: 12637 HaeII r_GCGC'y Cuts at: 1678 9731 11784 1678 Size: 8053 2053 2531 Fragments arranged by size: 8053 2531 2053 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2873 2905 3269 3301 4017 Size: 32 135 32 1301 32 364 32 716 Cuts at: 4017 4049 5330 5362 5827 5859 6029 6061 6568 Size: 32 1281 32 465 32 170 32 507 Cuts at: 6568 6600 6698 6730 7376 7408 9050 9082 9332 Size: 32 98 32 646 32 1642 32 250 Cuts at: 9332 9364 9622 9654 9738 9770 11278 11310 11352 Size: 32 258 32 84 32 1508 32 42 Cuts at: 11352 11384 1373 Size: 32 2626 Fragments arranged by size: 2626 1642 1508 1301 1281 716 646 507 465 364 258 250 170 135 98 84 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3566 3834 4142 6408 6728 8277 Size: 988 281 1628 268 308 2266 320 1549 Cuts at: 8277 9981 10227 669 Size: 1704 246 3079 Fragments arranged by size: 3079 2266 1704 1628 1549 988 320 308 281 268 246 HindIII A'AGCT_T Cuts at: 748 2928 4281 5920 6175 7516 8666 748 Size: 2180 1353 1639 255 1341 1150 4719 Fragments arranged by size: 4719 2180 1639 1353 1341 1150 255 HpaI GTT'AAC Cuts at: 8277 10227 8277 Size: 1950 10687 KpnI G_GTAC'C Cuts at: 4661 8832 9962 4661 Size: 4171 1130 7336 Fragments arranged by size: 7336 4171 1130 MfeI C'AATT_G Cuts at: 2658 2864 6326 8435 9490 10236 2658 Size: 206 3462 2109 1055 746 5059 Fragments arranged by size: 5059 3462 2109 1055 746 206 MluI A'CGCG_T Cuts at: 12513 12513 Size: 12637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7563 7744 8620 9902 11751 11935 1305 Size: 6258 181 876 1282 1849 184 2007 Fragments arranged by size: 6258 2007 1849 1282 876 184 181 MscI TGG'CCA Cuts at: 10 64 1489 1832 2518 10 Size: 54 1425 343 686 10129 Fragments arranged by size: 10129 1425 686 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 3793 3813 4212 5889 6554 7212 Size: 648 2463 164 20 399 1677 665 658 Cuts at: 7212 7268 8311 8573 8975 10595 10954 11113 518 Size: 56 1043 262 402 1620 359 159 2042 Fragments arranged by size: 2463 2042 1677 1620 1043 665 658 648 402 399 359 262 164 159 56 20 MspA1I CmG'CkG Cuts at: 1606 3080 6254 7249 7511 8546 10053 10730 11878 Size: 1474 3174 995 262 1035 1507 677 1148 Cuts at: 11878 12123 1606 Size: 245 2120 Fragments arranged by size: 3174 2120 1507 1474 1148 1035 995 677 262 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3794 7213 7999 8157 513 Size: 552 939 1790 3419 786 158 4993 Fragments arranged by size: 4993 3419 1790 939 786 552 158 NdeI CA'TA_TG Cuts at: 387 8308 8613 9097 387 Size: 7921 305 484 3927 Fragments arranged by size: 7921 3927 484 305 NheI G'CTAG_C Cuts at: 1052 2801 8846 1052 Size: 1749 6045 4843 Fragments arranged by size: 6045 4843 1749 NotI GC'GGCC_GC Cuts at: 10084 10084 Size: 12637 NsiI A_TGCA'T Cuts at: 7056 7056 Size: 12637 NspI r_CATG'y Cuts at: 1132 1229 3365 4168 4217 4574 5413 5504 6353 Size: 97 2136 803 49 357 839 91 849 Cuts at: 6353 7286 7573 7993 9482 9612 9863 9995 11540 Size: 933 287 420 1489 130 251 132 1545 Cuts at: 11540 1132 Size: 2229 Fragments arranged by size: 2229 2136 1545 1489 933 849 839 803 420 357 287 251 132 130 97 91 49 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 12637 PciI A'CATG_T Cuts at: 3361 4213 5500 7569 7989 9478 9859 11536 3361 Size: 852 1287 2069 420 1489 381 1677 4462 Fragments arranged by size: 4462 2069 1677 1489 1287 852 420 381 PflMI CCAn_nnn'nTGG Cuts at: 1108 8864 1108 Size: 7756 4881 PfoI T'CCnGG_A Cuts at: 3762 5808 8863 3762 Size: 2046 3055 7536 Fragments arranged by size: 7536 3055 2046 PmeI GTTT'AAAC Cuts at: 9941 9941 Size: 12637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10706 10738 12219 12251 10706 Size: 32 1481 32 11092 Fragments arranged by size: 11092 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5492 9575 5492 Size: 4083 8554 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12637 PsiI TTA'TAA Cuts at: 4529 10207 4529 Size: 5678 6959 PspOMI G'GGCC_C Cuts at: 3184 3978 5329 3184 Size: 794 1351 10492 Fragments arranged by size: 10492 1351 794 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4401 4433 7482 7514 4401 Size: 32 3049 32 9524 Fragments arranged by size: 9524 3049 32 32 PstI C_TGCA'G Cuts at: 830 3057 3987 5032 5698 7782 9391 9989 830 Size: 2227 930 1045 666 2084 1609 598 3478 Fragments arranged by size: 3478 2227 2084 1609 1045 930 666 598 PvuI CG_AT'CG Cuts at: 2001 8338 2001 Size: 6337 6300 PvuII CAG'CTG Cuts at: 3080 6254 7249 7511 8546 3080 Size: 3174 995 262 1035 7171 Fragments arranged by size: 7171 3174 1035 995 262 SacI G_AGCT'C Cuts at: 3992 4744 5426 6244 3992 Size: 752 682 818 10385 Fragments arranged by size: 10385 818 752 682 SalI G'TCGA_C Cuts at: 1936 9979 1936 Size: 8043 4594 SanDI GG'GwC_CC Cuts at: 5492 5492 Size: 12637 SapI GCTCTTCn'nnn_ Cuts at: 2874 9598 2874 Size: 6724 5913 SbfI CC_TGCA'GG Cuts at: 9989 9989 Size: 12637 ScaI AGT'ACT Cuts at: 3176 8138 10885 12597 3176 Size: 4962 2747 1712 3216 Fragments arranged by size: 4962 3216 2747 1712 SfcI C'TryA_G Cuts at: 826 1045 2160 3053 3591 3643 3868 3983 4228 Size: 219 1115 893 538 52 225 115 245 Cuts at: 4228 5028 5694 6292 6544 7684 7778 9387 9985 Size: 800 666 598 252 1140 94 1609 598 Cuts at: 9985 11120 11801 11992 826 Size: 1135 681 191 1471 Fragments arranged by size: 1609 1471 1140 1135 1115 893 800 681 666 598 598 538 252 245 225 219 191 115 94 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12637 SmaI CCC'GGG Cuts at: 1872 9964 1872 Size: 8092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3014 3635 3950 4546 6048 6132 Size: 197 926 1071 621 315 596 1502 84 Cuts at: 6132 6243 6442 7632 9049 10741 11642 11904 820 Size: 111 199 1190 1417 1692 901 262 1553 Fragments arranged by size: 1692 1553 1502 1417 1190 1071 926 901 621 596 315 262 199 197 111 84 SnaBI TAC'GTA Cuts at: 493 10428 493 Size: 9935 2702 SpeI A'CTAG_T Cuts at: 152 7477 8017 152 Size: 7325 540 4772 Fragments arranged by size: 7325 4772 540 SphI G_CATG'C Cuts at: 1132 7286 9995 1132 Size: 6154 2709 3774 Fragments arranged by size: 6154 3774 2709 SspI AAT'ATT Cuts at: 5 52 2941 3127 3945 7009 9445 10561 5 Size: 47 2889 186 818 3064 2436 1116 2081 Fragments arranged by size: 3064 2889 2436 2081 1116 818 186 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 12637 StyI C'CwwG_G Cuts at: 513 1065 2004 3794 4506 5221 7138 7213 7692 Size: 552 939 1790 712 715 1917 75 479 Cuts at: 7692 7899 7999 8157 10076 513 Size: 207 100 158 1919 3074 Fragments arranged by size: 3074 1919 1917 1790 939 715 712 552 479 207 158 100 75 SwaI ATTT'AAAT Cuts at: 2958 2958 Size: 12637 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3304 9130 10664 10681 10834 11019 11464 Size: 1255 1724 5826 1534 17 153 185 445 Cuts at: 11464 325 Size: 1498 Fragments arranged by size: 5826 1724 1534 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 3174 3512 Size: 274 80 33 51 1443 115 1082 338 Cuts at: 3512 4151 6734 8136 8341 10883 12595 96 Size: 639 2583 1402 205 2542 1712 138 Fragments arranged by size: 2583 2542 1712 1443 1402 1082 639 338 274 205 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 12597 12629 12597 Size: 32 12605 Tth111I GACn'n_nGTC Cuts at: 1316 4109 1316 Size: 2793 9844 XbaI T'CTAG_A Cuts at: 6901 8671 9973 6901 Size: 1770 1302 9565 Fragments arranged by size: 9565 1770 1302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3697 9914 3697 Size: 6217 6420 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12637 XmaI C'CCGG_G Cuts at: 1870 9962 1870 Size: 8092 4545 XmnI GAAnn'nnTTC Cuts at: 2769 3420 3663 4824 8704 8859 9444 10766 2769 Size: 651 243 1161 3880 155 585 1322 4640 Fragments arranged by size: 4640 3880 1322 1161 651 585 243 155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10445 276 Size: 53 83 186 936 8911 2468 Fragments arranged by size: 8911 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacII SexAI SfiI SfoI SrfI