Restriction map for
pFN21AE1086
(Linear) (Six-base) MAPSORT of: KIEE1086.seq Check: 5158 from: 1 to: 7932
KIEE1086.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4543 7932
Size: 4543 3389
AccI GT'mk_AC
Cuts at: 0 4841 5248 7008 7932
Size: 4841 407 1760 924
Fragments arranged by size:
4841 1760 924 407
Acc65I G'GTAC_C
Cuts at: 0 2652 6823 7932
Size: 2652 4171 1109
Fragments arranged by size:
4171 2652 1109
AclI AA'CG_TT
Cuts at: 0 2939 7932
Size: 2939 4993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 2531 2614 3187 3826 3838 6901 7932
Size: 1402 1129 83 573 639 12 3063 1031
Fragments arranged by size:
3063 1402 1129 1031 639 573 83 12
AflII C'TTAA_G
Cuts at: 0 1009 1630 2541 4437 7044 7932
Size: 1009 621 911 1896 2607 888
Fragments arranged by size:
2607 1896 1009 911 888 621
AflIII A'CryG_T
Cuts at: 0 1356 2208 3495 5312 5564 5984 7473 7854
Size: 1356 852 1287 1817 252 420 1489 381
Cuts at: 7854 7932
Size: 78
Fragments arranged by size:
1817 1489 1356 1287 852 420 381 252
78
AgeI A'CCGG_T
Cuts at: 0 6684 7932
Size: 6684 1248
AleI CACnn'nnGTG
Cuts at: 0 1624 5263 7932
Size: 1624 3639 2669
Fragments arranged by size:
3639 2669 1624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1053 7932
Size: 1053 6879
AlwNI CAG_nnn'CTG
Cuts at: 0 1668 2704 3031 3414 4794 7932
Size: 1668 1036 327 383 1380 3138
Fragments arranged by size:
3138 1668 1380 1036 383 327
ApaI G_GGCC'C
Cuts at: 0 1183 1977 3328 7932
Size: 1183 794 1351 4604
Fragments arranged by size:
4604 1351 1183 794
ApaLI G'TGCA_C
Cuts at: 0 1391 7932
Size: 1391 6541
ApoI r'AATT_y
Cuts at: 0 104 292 600 607 1063 1703 2046 2116
Size: 104 188 308 7 456 640 343 70
Cuts at: 2116 2430 4526 4783 5673 6443 6902 7018 7276
Size: 314 2096 257 890 770 459 116 258
Cuts at: 7276 7932
Size: 656
Fragments arranged by size:
2096 890 770 656 640 459 456 343
314 308 258 257 188 116 104 70
7
AseI AT'TA_AT
Cuts at: 0 7034 7932
Size: 7034 898
AvaI C'yCGr_G
Cuts at: 0 3327 7932
Size: 3327 4605
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4374 4407 7341 7374 7932
Size: 4374 33 2934 33 558
Fragments arranged by size:
4374 2934 558 33 33
BanI G'GyrC_C
Cuts at: 0 2652 6823 7932
Size: 2652 4171 1109
Fragments arranged by size:
4171 2652 1109
BanII G_rGCy'C
Cuts at: 0 1183 1977 1987 2739 3328 3421 3628 4239
Size: 1183 794 10 752 589 93 207 611
Cuts at: 4239 7932
Size: 3693
Fragments arranged by size:
3693 1183 794 752 611 589 207 93
10
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 995 4877 7932
Size: 467 528 3882 3055
Fragments arranged by size:
3882 3055 528 467
BbvCI CC'TCA_GC
Cuts at: 0 1258 5143 7932
Size: 1258 3885 2789
Fragments arranged by size:
3885 2789 1258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 7932
Size: 303 34 7595
Fragments arranged by size:
7595 303 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 122 843 2592 4908 7932
Size: 122 721 1749 2316 3024
Fragments arranged by size:
3024 2316 1749 721 122
BclI T'GATC_A
Cuts at: 0 3768 7632 7932
Size: 3768 3864 300
Fragments arranged by size:
3864 3768 300
BfrBI ATG'CAT
Cuts at: 0 5049 7932
Size: 5049 2883
BglII A'GATC_T
Cuts at: 0 6220 7932
Size: 6220 1712
Bme1580I G_kGCm'C
Cuts at: 0 1183 1395 1977 3156 3328 7932
Size: 1183 212 582 1179 172 4604
Fragments arranged by size:
4604 1183 1179 582 212 172
BmgBI CAC'GTC
Cuts at: 0 2874 7932
Size: 2874 5058
BmrI ACTGGGnnnn_n'
Cuts at: 0 1980 3199 4621 5419 5655 7932
Size: 1980 1219 1422 798 236 2277
Fragments arranged by size:
2277 1980 1422 1219 798 236
BmtI G_CTAG'C
Cuts at: 0 800 6845 7932
Size: 800 6045 1087
Fragments arranged by size:
6045 1087 800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7297 7932
Size: 7297 635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1696 2732 3787 4175 4436 5597 7760
Size: 37 1659 1036 1055 388 261 1161 2163
Cuts at: 7760 7932
Size: 172
Fragments arranged by size:
2163 1659 1161 1055 1036 388 261 172
37
Bpu10I CC'TnA_GC
Cuts at: 0 1258 4340 5143 7932
Size: 1258 3082 803 2789
Fragments arranged by size:
3082 2789 1258 803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1966 4028 4112 4223 5612 7932
Size: 1966 2062 84 111 1389 2320
Fragments arranged by size:
2320 2062 1966 1389 111 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 3814 4294 7152 7415 7932
Size: 3814 480 2858 263 517
Fragments arranged by size:
3814 2858 517 480 263
BsaAI yAC'GTr
Cuts at: 0 5315 5868 7932
Size: 5315 553 2064
Fragments arranged by size:
5315 2064 553
BsaBI GATnn'nnATC
Cuts at: 0 2960 3317 7932
Size: 2960 357 4615
Fragments arranged by size:
4615 2960 357
BsaHI Gr'CG_yC
Cuts at: 0 1293 7932
Size: 1293 6639
BsaWI w'CCGG_w
Cuts at: 0 125 5708 6194 6684 7932
Size: 125 5583 486 490 1248
Fragments arranged by size:
5583 1248 490 486 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2636 2666 7733 7763 7932
Size: 2636 30 5067 30 169
Fragments arranged by size:
5067 2636 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1240 4344 7932
Size: 1240 3104 3588
Fragments arranged by size:
3588 3104 1240
BseYI C'CCAG_C
Cuts at: 0 51 1075 7932
Size: 51 1024 6857
Fragments arranged by size:
6857 1024 51
BsiEI CG_ry'CG
Cuts at: 0 6333 7932
Size: 6333 1599
BsiHKAI G_wGCw'C
Cuts at: 0 1001 1332 1395 1987 2739 3421 3835 4239
Size: 1001 331 63 592 752 682 414 404
Cuts at: 4239 6721 7932
Size: 2482 1211
Fragments arranged by size:
2482 1211 1001 752 682 592 414 404
331 63
BsmI GAATG_Cn'
Cuts at: 0 1781 2871 5607 5770 7471 7713 7932
Size: 1781 1090 2736 163 1701 242 219
Fragments arranged by size:
2736 1781 1701 1090 242 219 163
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1303 1313 7932
Size: 1303 10 6619
Fragments arranged by size:
6619 1303 10
Bsp1286I G_dGCh'C
Cuts at: 0 1001 1183 1332 1395 1977 1987 2739 3156
Size: 1001 182 149 63 582 10 752 417
Cuts at: 3156 3328 3421 3628 3835 4239 6721 7932
Size: 172 93 207 207 404 2482 1211
Fragments arranged by size:
2482 1211 1001 752 582 417 404 207
207 182 172 149 93 63 10
BspEI T'CCGG_A
Cuts at: 0 5708 7932
Size: 5708 2224
BspHI T'CATG_A
Cuts at: 0 224 521 3747 6794 6968 7308 7932
Size: 224 297 3226 3047 174 340 624
Fragments arranged by size:
3226 3047 624 340 297 224 174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4474 4543 7932
Size: 4474 69 3389
Fragments arranged by size:
4474 3389 69
BsrBI CCG'CTC
Cuts at: 0 6326 7932
Size: 6326 1606
BsrDI GCAATG_nn'
Cuts at: 0 4703 6060 6530 7932
Size: 4703 1357 470 1402
Fragments arranged by size:
4703 1402 1357 470
BsrFI r'CCGG_y
Cuts at: 0 6684 7932
Size: 6684 1248
BsrGI T'GTAC_A
Cuts at: 0 87 6336 7932
Size: 87 6249 1596
Fragments arranged by size:
6249 1596 87
BssSI C'ACGA_G
Cuts at: 0 4955 7932
Size: 4955 2977
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3022 3031 7932
Size: 3022 9 4901
Fragments arranged by size:
4901 3022 9
BstBI TT'CG_AA
Cuts at: 0 3042 7086 7596 7932
Size: 3042 4044 510 336
Fragments arranged by size:
4044 3042 510 336
BstEII G'GTnAC_C
Cuts at: 0 4315 5803 7932
Size: 4315 1488 2129
Fragments arranged by size:
4315 2129 1488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3381 7932
Size: 3381 4551
BstYI r'GATC_y
Cuts at: 0 2442 4092 5114 6220 7932
Size: 2442 1650 1022 1106 1712
Fragments arranged by size:
2442 1712 1650 1106 1022
Bsu36I CC'TnA_GG
Cuts at: 0 5972 7932
Size: 5972 1960
BtgI C'CryG_G
Cuts at: 0 1789 5208 5994 6152 7932
Size: 1789 3419 786 158 1780
Fragments arranged by size:
3419 1789 1780 786 158
BtsI GCAGTG_nn'
Cuts at: 0 63 5153 7145 7379 7932
Size: 63 5090 1992 234 553
Fragments arranged by size:
5090 1992 553 234 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6508 6543 7932
Size: 6508 35 1389
Fragments arranged by size:
6508 1389 35
DraI TTT'AAA
Cuts at: 0 402 953 1823 2915 6729 6941 7932
Size: 402 551 870 1092 3814 212 991
Fragments arranged by size:
3814 1092 991 870 551 402 212
DraIII CAC_nnn'GTG
Cuts at: 0 1906 4769 7932
Size: 1906 2863 3163
Fragments arranged by size:
3163 2863 1906
DrdI GACnn_nn'nnGTC
Cuts at: 0 6543 7932
Size: 6543 1389
EaeI y'GGCC_r
Cuts at: 0 511 5066 6321 7932
Size: 511 4555 1255 1611
Fragments arranged by size:
4555 1611 1255 511
EarI CTCTTCn'nnn_
Cuts at: 0 869 2156 2224 3857 6920 7065 7593 7932
Size: 869 1287 68 1633 3063 145 528 339
Fragments arranged by size:
3063 1633 1287 869 528 339 145 68
EcoICRI GAG'CTC
Cuts at: 0 1985 2737 3419 4237 7932
Size: 1985 752 682 818 3695
Fragments arranged by size:
3695 1985 818 752 682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1402 1696 2531 2614 2732 3187 3787
Size: 37 1365 294 835 83 118 455 600
Cuts at: 3787 3826 3838 4175 4436 5597 6901 7760 7932
Size: 39 12 337 261 1161 1304 859 172
Fragments arranged by size:
1365 1304 1161 859 835 600 455 337
294 261 172 118 83 39 37 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6997 7932
Size: 6997 935
EcoO109I rG'GnC_Cy
Cuts at: 0 1974 3324 3487 7570 7932
Size: 1974 1350 163 4083 362
Fragments arranged by size:
4083 1974 1350 362 163
EcoRI G'AATT_C
Cuts at: 0 292 2046 5673 7018 7932
Size: 292 1754 3627 1345 914
Fragments arranged by size:
3627 1754 1345 914 292
EcoRV GAT'ATC
Cuts at: 0 3336 5445 7932
Size: 3336 2109 2487
Fragments arranged by size:
3336 2487 2109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2431 2619 3760 3945 7932
Size: 2431 188 1141 185 3987
Fragments arranged by size:
3987 2431 1141 188 185
HaeII r_GCGC'y
Cuts at: 0 7726 7932
Size: 7726 206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 1264 1296 2012 2044 3325 3357
Size: 868 32 364 32 716 32 1281 32
Cuts at: 3357 3822 3854 4024 4056 4563 4595 4693 4725
Size: 465 32 170 32 507 32 98 32
Cuts at: 4725 5371 5403 7045 7077 7327 7359 7617 7649
Size: 646 32 1642 32 250 32 258 32
Cuts at: 7649 7733 7765 7932
Size: 84 32 167
Fragments arranged by size:
1642 1281 868 716 646 507 465 364
258 250 170 167 98 84 32 32
32 32 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1561 1829 2137 4403 4723 6272 7932
Size: 1561 268 308 2266 320 1549 1660
Fragments arranged by size:
2266 1660 1561 1549 320 308 268
HindIII A'AGCT_T
Cuts at: 0 923 2276 3915 4170 5511 6661 7932
Size: 923 1353 1639 255 1341 1150 1271
Fragments arranged by size:
1639 1353 1341 1271 1150 923 255
HpaI GTT'AAC
Cuts at: 0 6272 7932
Size: 6272 1660
KpnI G_GTAC'C
Cuts at: 0 2656 6827 7932
Size: 2656 4171 1105
Fragments arranged by size:
4171 2656 1105
MfeI C'AATT_G
Cuts at: 0 653 859 4321 6430 7485 7932
Size: 653 206 3462 2109 1055 447
Fragments arranged by size:
3462 2109 1055 653 447 206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5558 5739 6615 7897 7932
Size: 5558 181 876 1282 35
Fragments arranged by size:
5558 1282 876 181 35
MscI TGG'CCA
Cuts at: 0 513 7932
Size: 513 7419
MslI CAynn'nnrTG
Cuts at: 0 1624 1788 1808 2207 3884 4549 5207 5263
Size: 1624 164 20 399 1677 665 658 56
Cuts at: 5263 6306 6568 6970 7932
Size: 1043 262 402 962
Fragments arranged by size:
1677 1624 1043 962 665 658 402 399
262 164 56 20
MspA1I CmG'CkG
Cuts at: 0 1075 4249 5244 5506 6541 7932
Size: 1075 3174 995 262 1035 1391
Fragments arranged by size:
3174 1391 1075 1035 995 262
NcoI C'CATG_G
Cuts at: 0 1789 5208 5994 6152 7932
Size: 1789 3419 786 158 1780
Fragments arranged by size:
3419 1789 1780 786 158
NdeI CA'TA_TG
Cuts at: 0 6303 6608 7092 7932
Size: 6303 305 484 840
Fragments arranged by size:
6303 840 484 305
NheI G'CTAG_C
Cuts at: 0 796 6841 7932
Size: 796 6045 1091
Fragments arranged by size:
6045 1091 796
NsiI A_TGCA'T
Cuts at: 0 5051 7932
Size: 5051 2881
NspI r_CATG'y
Cuts at: 0 1360 2163 2212 2569 3408 3499 4348 5281
Size: 1360 803 49 357 839 91 849 933
Cuts at: 5281 5568 5988 7477 7607 7858 7932
Size: 287 420 1489 130 251 74
Fragments arranged by size:
1489 1360 933 849 839 803 420 357
287 251 130 91 74 49
PciI A'CATG_T
Cuts at: 0 1356 2208 3495 5564 5984 7473 7854 7932
Size: 1356 852 1287 2069 420 1489 381 78
Fragments arranged by size:
2069 1489 1356 1287 852 420 381 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 6859 7932
Size: 6859 1073
PfoI T'CCnGG_A
Cuts at: 0 1757 3803 6858 7932
Size: 1757 2046 3055 1074
Fragments arranged by size:
3055 2046 1757 1074
PpuMI rG'GwC_Cy
Cuts at: 0 3487 7570 7932
Size: 3487 4083 362
Fragments arranged by size:
4083 3487 362
PsiI TTA'TAA
Cuts at: 0 2524 7932
Size: 2524 5408
PspOMI G'GGCC_C
Cuts at: 0 1179 1973 3324 7932
Size: 1179 794 1351 4608
Fragments arranged by size:
4608 1351 1179 794
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2396 2428 5477 5509 7932
Size: 2396 32 3049 32 2423
Fragments arranged by size:
3049 2423 2396 32 32
PstI C_TGCA'G
Cuts at: 0 1052 1982 3027 3693 5777 7386 7932
Size: 1052 930 1045 666 2084 1609 546
Fragments arranged by size:
2084 1609 1052 1045 930 666 546
PvuI CG_AT'CG
Cuts at: 0 6333 7932
Size: 6333 1599
PvuII CAG'CTG
Cuts at: 0 1075 4249 5244 5506 6541 7932
Size: 1075 3174 995 262 1035 1391
Fragments arranged by size:
3174 1391 1075 1035 995 262
SacI G_AGCT'C
Cuts at: 0 1987 2739 3421 4239 7932
Size: 1987 752 682 818 3693
Fragments arranged by size:
3693 1987 818 752 682
SanDI GG'GwC_CC
Cuts at: 0 3487 7932
Size: 3487 4445
SapI GCTCTTCn'nnn_
Cuts at: 0 869 7593 7932
Size: 869 6724 339
Fragments arranged by size:
6724 869 339
ScaI AGT'ACT
Cuts at: 0 1171 6133 7932
Size: 1171 4962 1799
Fragments arranged by size:
4962 1799 1171
SfcI C'TryA_G
Cuts at: 0 155 1048 1586 1638 1863 1978 2223 3023
Size: 155 893 538 52 225 115 245 800
Cuts at: 3023 3689 4287 4539 5679 5773 7382 7932
Size: 666 598 252 1140 94 1609 550
Fragments arranged by size:
1609 1140 893 800 666 598 550 538
252 245 225 155 115 94 52
SmlI C'TyrA_G
Cuts at: 0 1009 1630 1945 2541 4043 4127 4238 4437
Size: 1009 621 315 596 1502 84 111 199
Cuts at: 4437 5627 7044 7932
Size: 1190 1417 888
Fragments arranged by size:
1502 1417 1190 1009 888 621 596 315
199 111 84
SpeI A'CTAG_T
Cuts at: 0 5472 6012 7932
Size: 5472 540 1920
Fragments arranged by size:
5472 1920 540
SphI G_CATG'C
Cuts at: 0 5281 7932
Size: 5281 2651
SspI AAT'ATT
Cuts at: 0 936 1122 1940 5004 7440 7932
Size: 936 186 818 3064 2436 492
Fragments arranged by size:
3064 2436 936 818 492 186
StyI C'CwwG_G
Cuts at: 0 1789 2501 3216 5133 5208 5687 5894 5994
Size: 1789 712 715 1917 75 479 207 100
Cuts at: 5994 6152 7932
Size: 158 1780
Fragments arranged by size:
1917 1789 1780 715 712 479 207 158
100 75
SwaI ATTT'AAAT
Cuts at: 0 953 7932
Size: 953 6979
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1299 7125 7932
Size: 1299 5826 807
Fragments arranged by size:
5826 1299 807
TatI w'GTAC_w
Cuts at: 0 87 1169 1507 2146 4729 6131 6336 7932
Size: 87 1082 338 639 2583 1402 205 1596
Fragments arranged by size:
2583 1596 1402 1082 639 338 205 87
Tth111I GACn'n_nGTC
Cuts at: 0 2104 7932
Size: 2104 5828
XbaI T'CTAG_A
Cuts at: 0 4896 6666 7932
Size: 4896 1770 1266
Fragments arranged by size:
4896 1770 1266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1692 7909 7932
Size: 1692 6217 23
Fragments arranged by size:
6217 1692 23
XmnI GAAnn'nnTTC
Cuts at: 0 764 1415 1658 2819 6699 6854 7439 7932
Size: 764 651 243 1161 3880 155 585 493
Fragments arranged by size:
3880 1161 764 651 585 493 243 155
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MmeI MscI MslI MspA1I NcoI
NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SanDI
SapI ScaI SfcI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AsiSI AvrII BamHI
BbeI BglI BlpI BsgI BsiWI BssHII BstZ17I BtgZI
ClaI EagI EciI FseI FspI FspAI KasI MluI
NaeI NarI NgoMIV NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PspXI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
StuI TstI XhoI XmaI ZraI