Restriction map for
pFN21AB9961
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I AviII AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BseAI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI Ksp632I MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 871 GGTACC G^GTACC
AcsI 1276 AAATTC [GA]^AATT[CT]
AflIII 1340 ACGTGT A^C[GA][CT]GT
Alw44I 70 GTGCAC G^TGCAC
ApaI 237 GGGCCC GGGCC^C
ApaLI 70 GTGCAC G^TGCAC
ApoI 1276 AAATTC [GA]^AATT[CT]
Asp718I 871 GGTACC G^GTACC
AspI 211 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1355 GGATCC G^GATCC
BbsI 1243 GAAGAC GAAGAC(2/6)
BfaI 290 CTAG C^TAG
BlpI 378 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 378 GCTCAGC GC^T[ACGT]AGC
BpuAI 1243 GAAGAC GAAGAC(2/6)
BsaI 541 GAGACC GGTCTC(1/5)
BsaWI 1445 ACCGGA [AT]^CCGG[AT]
BsiWI 1133 CGTACG C^GTACG
BspHI 947 TCATGA T^CATGA
BsrFI 247 GCCGGT [GA]^CCGG[CT]
BssSI 526 CTCGTG CACGAG(-5/-1)
CelII 378 GCTCAGC GC^T[ACGT]AGC
Cfr10I 247 GCCGGT [GA]^CCGG[CT]
DraIII 735 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 300 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 340 GTCAAC GT[CT]^[GA]AC
HindII 340 GTCAAC GT[CT]^[GA]AC
KpnI 871 GGTACC GGTAC^C
MaeI 290 CTAG C^TAG
NotI 169 GCGGCCGC GC^GGCCGC
PflFI 211 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1283 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 473 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 947 TCATGA T^CATGA
SacI 1369 GAGCTC GAGCT^C
SfiI 136 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1594 CCCGGG CCC^GGG
SmlI 1057 CTCAAG C^T[CT][GA]AG
Tsp509I 1277 AATT ^AATT
Tth111I 211 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1283 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1594 CCCGGG C^CCGGG
XmaI 1594 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 38 enzymes) #
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AcyI 41 GGCGCC G[GA]^CG[CT]C
420 GGCGCC
AhdI 280 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1339 GACGTGTGGTC
AocI 1121 CCTCAGG CC^T[ACGT]AGG
1516 CCTGAGG
AspEI 280 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1339 GACGTGTGGTC
AspHI 70 GTGCAC G[AT]GC[AT]^C
1369 GAGCTC
BalI 773 TGGCCA TGG^CCA
884 TGGCCA
BbeI 41 GGCGCC GGCGC^C
420 GGCGCC
BglI 137 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
163 GCCCCCGCGGC
BsaAI 415 TACGTG [CT]AC^GT[GA]
1135 TACGTG
BsaHI 41 GGCGCC G[GA]^CG[CT]C
420 GGCGCC
BsiEI 128 CGGCCG CG[GA][CT]^CG
170 CGGCCG
BsiHKAI 70 GTGCAC G[AT]GC[AT]^C
1369 GAGCTC
BspMI 1079 ACCTGC ACCTGC(4/8)
1541 ACCTGC
Bsu36I 1121 CCTCAGG CC^T[ACGT]AGG
1516 CCTGAGG
DsaI 141 CCGCGG C^C[GA][CT]GG
167 CCGCGG
EagI 128 CGGCCG C^GGCCG
170 CGGCCG
Eam1105I 280 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1339 GACGTGTGGTC
EclXI 128 CGGCCG C^GGCCG
170 CGGCCG
Eco52I 128 CGGCCG C^GGCCG
170 CGGCCG
Eco81I 1121 CCTCAGG CC^T[ACGT]AGG
1516 CCTGAGG
EcoNI 771 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
930 CCTGCAGGAGG
Hin1I 41 GGCGCC G[GA]^CG[CT]C
420 GGCGCC
KasI 41 GGCGCC G^GCGCC
420 GGCGCC
KspI 141 CCGCGG CCGC^GG
167 CCGCGG
MboII 951 GAAGA GAAGA(8/7)
1243 GAAGA
MluNI 773 TGGCCA TGG^CCA
884 TGGCCA
MscI 773 TGGCCA TGG^CCA
884 TGGCCA
NarI 41 GGCGCC GG^CGCC
420 GGCGCC
PvuII 682 CAGCTG CAG^CTG
1093 CAGCTG
SacII 141 CCGCGG CCGC^GG
167 CCGCGG
SbfI 930 CCTGCAGG CCTGCA^GG
1542 CCTGCAGG
SexAI 1199 ACCTGGT A^CC[AT]GGT
1427 ACCAGGT
SfoI 41 GGCGCC GGC^GCC
420 GGCGCC
Sse8387I 930 CCTGCAGG CCTGCA^GG
1542 CCTGCAGG
StuI 923 AGGCCT AGG^CCT
1547 AGGCCT
TaqI 581 TCGA T^CGA
1535 TCGA
TspRI 569 CAGTG CA[GC]TG[ACGT][ACGT]^
1498 CAGTG
TthHB8I 581 TCGA T^CGA
1535 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I XcmI XhoII