Restriction map for
pFN21AB9961
(Linear) (Six-base) MAPSORT of: KIBB9961.seq Check: 5803 from: 1 to: 1608
KIBB9961.orf
With 182 enzymes: SgfI *
May 27, 2009 12:02 ..
Acc65I G'GTAC_C
Cuts at: 0 871 1608
Size: 871 737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1608
Size: 919 689
AflIII A'CryG_T
Cuts at: 0 1340 1608
Size: 1340 268
AhdI GACnn_n'nnGTC
Cuts at: 0 285 1344 1608
Size: 285 1059 264
Fragments arranged by size:
1059 285 264
AleI CACnn'nnGTG
Cuts at: 0 265 736 880 1608
Size: 265 471 144 728
Fragments arranged by size:
728 471 265 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1001 1096 1608
Size: 1001 95 512
Fragments arranged by size:
1001 512 95
AlwNI CAG_nnn'CTG
Cuts at: 0 574 684 771 1306 1608
Size: 574 110 87 535 302
Fragments arranged by size:
574 535 302 110 87
ApaI G_GGCC'C
Cuts at: 0 241 1608
Size: 241 1367
ApaLI G'TGCA_C
Cuts at: 0 70 1608
Size: 70 1538
ApoI r'AATT_y
Cuts at: 0 1276 1608
Size: 1276 332
AvaI C'yCGr_G
Cuts at: 0 510 797 1471 1594 1608
Size: 510 287 674 123 14
Fragments arranged by size:
674 510 287 123 14
BamHI G'GATC_C
Cuts at: 0 1355 1608
Size: 1355 253
BanI G'GyrC_C
Cuts at: 0 41 420 553 871 1270 1389 1608
Size: 41 379 133 318 399 119 219
Fragments arranged by size:
399 379 318 219 133 119 41
BanII G_rGCy'C
Cuts at: 0 59 182 241 1007 1373 1583 1608
Size: 59 123 59 766 366 210 25
Fragments arranged by size:
766 366 210 123 59 59 25
BbeI G_GCGC'C
Cuts at: 0 45 424 1608
Size: 45 379 1184
Fragments arranged by size:
1184 379 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1250 1608
Size: 1250 358
BbvCI CC'TCA_GC
Cuts at: 0 443 958 1091 1608
Size: 443 515 133 517
Fragments arranged by size:
517 515 443 133
BglI GCCn_nnn'nGGC
Cuts at: 0 143 169 1608
Size: 143 26 1439
Fragments arranged by size:
1439 143 26
BlpI GC'TnA_GC
Cuts at: 0 379 1608
Size: 379 1229
Bme1580I G_kGCm'C
Cuts at: 0 74 241 745 1394 1608
Size: 74 167 504 649 214
Fragments arranged by size:
649 504 214 167 74
BmgBI CAC'GTC
Cuts at: 0 749 1341 1571 1608
Size: 749 592 230 37
Fragments arranged by size:
749 592 230 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 657 1578 1608
Size: 657 921 30
Fragments arranged by size:
921 657 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 316 835 902 1283 1576 1608
Size: 73 243 519 67 381 293 32
Fragments arranged by size:
519 381 293 243 73 67 32
Bpu10I CC'TnA_GC
Cuts at: 0 443 958 1091 1608
Size: 443 515 133 517
Fragments arranged by size:
517 515 443 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1608
Size: 1042 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 535 1608
Size: 535 1073
BsaAI yAC'GTr
Cuts at: 0 417 1137 1608
Size: 417 720 471
Fragments arranged by size:
720 471 417
BsaHI Gr'CG_yC
Cuts at: 0 42 421 1608
Size: 42 379 1187
Fragments arranged by size:
1187 379 42
BsaWI w'CCGG_w
Cuts at: 0 1445 1608
Size: 1445 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 900 930 1608
Size: 637 30 233 30 678
Fragments arranged by size:
678 637 233 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 195 460 1015 1534 1608
Size: 195 265 555 519 74
Fragments arranged by size:
555 519 265 195 74
BseYI C'CCAG_C
Cuts at: 0 112 148 406 825 1608
Size: 112 36 258 419 783
Fragments arranged by size:
783 419 258 112 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1193 1465 1608
Size: 997 196 272 143
Fragments arranged by size:
997 272 196 143
BsiEI CG_ry'CG
Cuts at: 0 131 173 1608
Size: 131 42 1435
Fragments arranged by size:
1435 131 42
BsiHKAI G_wGCw'C
Cuts at: 0 74 1373 1608
Size: 74 1299 235
Fragments arranged by size:
1299 235 74
BsiWI C'GTAC_G
Cuts at: 0 1133 1608
Size: 1133 475
Bsp1286I G_dGCh'C
Cuts at: 0 59 74 182 241 745 1007 1373 1394
Size: 59 15 108 59 504 262 366 21
Cuts at: 1394 1583 1608
Size: 189 25
Fragments arranged by size:
504 366 262 189 108 59 59 25
21 15
BspHI T'CATG_A
Cuts at: 0 947 1608
Size: 947 661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1088 1550 1608
Size: 1088 462 58
Fragments arranged by size:
1088 462 58
BsrBI CCG'CTC
Cuts at: 0 327 486 1143 1437 1524 1608
Size: 327 159 657 294 87 84
Fragments arranged by size:
657 327 294 159 87 84
BsrFI r'CCGG_y
Cuts at: 0 247 1608
Size: 247 1361
BssSI C'ACGA_G
Cuts at: 0 526 1608
Size: 526 1082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 882 1185 1608
Size: 267 615 303 423
Fragments arranged by size:
615 423 303 267
BstYI r'GATC_y
Cuts at: 0 617 791 1355 1608
Size: 617 174 564 253
Fragments arranged by size:
617 564 253 174
Bsu36I CC'TnA_GG
Cuts at: 0 1122 1517 1608
Size: 1122 395 91
Fragments arranged by size:
1122 395 91
BtgI C'CryG_G
Cuts at: 0 141 167 1608
Size: 141 26 1441
Fragments arranged by size:
1441 141 26
DraIII CAC_nnn'GTG
Cuts at: 0 740 1608
Size: 740 868
DrdI GACnn_nn'nnGTC
Cuts at: 0 306 1608
Size: 306 1302
EaeI y'GGCC_r
Cuts at: 0 128 135 170 245 773 884 1214 1317
Size: 128 7 35 75 528 111 330 103
Cuts at: 1317 1608
Size: 291
Fragments arranged by size:
528 330 291 128 111 103 75 35
7
EagI C'GGCC_G
Cuts at: 0 128 170 1608
Size: 128 42 1438
Fragments arranged by size:
1438 128 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1521 1608
Size: 1521 87
EcoICRI GAG'CTC
Cuts at: 0 1371 1608
Size: 1371 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 316 835 902 919 1283 1576 1608
Size: 73 243 519 67 17 364 293 32
Fragments arranged by size:
519 364 293 243 73 67 32 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 775 934 1608
Size: 775 159 674
Fragments arranged by size:
775 674 159
EcoO109I rG'GnC_Cy
Cuts at: 0 518 594 768 860 1164 1608
Size: 518 76 174 92 304 444
Fragments arranged by size:
518 444 304 174 92 76
HaeII r_GCGC'y
Cuts at: 0 45 80 92 424 1608
Size: 45 35 12 332 1184
Fragments arranged by size:
1184 332 45 35 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1330 1362 1608
Size: 271 32 1027 32 246
Fragments arranged by size:
1027 271 246 32 32
HincII GTy'rAC
Cuts at: 0 342 1608
Size: 342 1266
KasI G'GCGC_C
Cuts at: 0 41 420 1608
Size: 41 379 1188
Fragments arranged by size:
1188 379 41
KpnI G_GTAC'C
Cuts at: 0 875 1608
Size: 875 733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1316 1608
Size: 452 864 292
Fragments arranged by size:
864 452 292
MscI TGG'CCA
Cuts at: 0 775 886 1608
Size: 775 111 722
Fragments arranged by size:
775 722 111
MslI CAynn'nnrTG
Cuts at: 0 265 710 736 880 1186 1608
Size: 265 445 26 144 306 422
Fragments arranged by size:
445 422 306 265 144 26
MspA1I CmG'CkG
Cuts at: 0 39 143 169 243 635 684 1095 1608
Size: 39 104 26 74 392 49 411 513
Fragments arranged by size:
513 411 392 104 74 49 39 26
NarI GG'CG_CC
Cuts at: 0 42 421 1608
Size: 42 379 1187
Fragments arranged by size:
1187 379 42
NotI GC'GGCC_GC
Cuts at: 0 170 1608
Size: 170 1438
PasI CC'CwG_GG
Cuts at: 0 1399 1608
Size: 1399 209
PflMI CCAn_nnn'nTGG
Cuts at: 0 1289 1608
Size: 1289 319
PfoI T'CCnGG_A
Cuts at: 0 1553 1608
Size: 1553 55
PpuMI rG'GwC_Cy
Cuts at: 0 518 860 1164 1608
Size: 518 342 304 444
Fragments arranged by size:
518 444 342 304
PshAI GACnn'nnGTC
Cuts at: 0 477 1608
Size: 477 1131
PspOMI G'GGCC_C
Cuts at: 0 237 1608
Size: 237 1371
PstI C_TGCA'G
Cuts at: 0 680 935 1547 1608
Size: 680 255 612 61
Fragments arranged by size:
680 612 255 61
PvuII CAG'CTG
Cuts at: 0 684 1095 1608
Size: 684 411 513
Fragments arranged by size:
684 513 411
SacI G_AGCT'C
Cuts at: 0 1373 1608
Size: 1373 235
SacII CC_GC'GG
Cuts at: 0 144 170 1608
Size: 144 26 1438
Fragments arranged by size:
1438 144 26
SbfI CC_TGCA'GG
Cuts at: 0 935 1547 1608
Size: 935 612 61
Fragments arranged by size:
935 612 61
SexAI A'CCwGG_T
Cuts at: 0 1199 1427 1608
Size: 1199 228 181
Fragments arranged by size:
1199 228 181
SfcI C'TryA_G
Cuts at: 0 676 931 1543 1608
Size: 676 255 612 65
Fragments arranged by size:
676 612 255 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 143 1608
Size: 143 1465
SfoI GGC'GCC
Cuts at: 0 43 422 1608
Size: 43 379 1186
Fragments arranged by size:
1186 379 43
SmaI CCC'GGG
Cuts at: 0 1596 1608
Size: 1596 12
SmlI C'TyrA_G
Cuts at: 0 1057 1608
Size: 1057 551
StuI AGG'CCT
Cuts at: 0 925 1549 1608
Size: 925 624 59
Fragments arranged by size:
925 624 59
StyI C'CwwG_G
Cuts at: 0 23 590 776 1608
Size: 23 567 186 832
Fragments arranged by size:
832 567 186 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 1608
Size: 676 932
TatI w'GTAC_w
Cuts at: 0 1025 1253 1608
Size: 1025 228 355
Fragments arranged by size:
1025 355 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 900 932 1608
Size: 900 32 676
Fragments arranged by size:
900 676 32
Tth111I GACn'n_nGTC
Cuts at: 0 214 1608
Size: 214 1394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 838 1185 1608
Size: 414 424 347 423
Fragments arranged by size:
424 423 414 347
XmaI C'CCGG_G
Cuts at: 0 1594 1608
Size: 1594 14
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI
BsrFI BssSI BstXI BstYI Bsu36I BtgI DraIII DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NarI NotI PasI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SbfI SexAI SfcI SfiI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AloI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BmtI BplI BsaBI BsmI BsmBI BspEI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI EarI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI ScaI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI
ZraI