Restriction map for
pFN21AB3024
(Linear) (Six-base) MAPSORT of: KIBB3024.seq Check: 7486 from: 1 to: 4056
KIBB3024.orf
With 182 enzymes: SgfI *
December 26, 2011 13:17 ..
AatII G_ACGT'C
Cuts at: 0 2248 4056
Size: 2248 1808
AccI GT'mk_AC
Cuts at: 0 1156 2492 3143 4056
Size: 1156 1336 651 913
Fragments arranged by size:
1336 1156 913 651
Acc65I G'GTAC_C
Cuts at: 0 3363 4056
Size: 3363 693
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3509 3912 4056
Size: 3509 403 144
Fragments arranged by size:
3509 403 144
AfeI AGC'GCT
Cuts at: 0 3244 4056
Size: 3244 812
AflIII A'CryG_T
Cuts at: 0 1121 4056
Size: 1121 2935
AhdI GACnn_n'nnGTC
Cuts at: 0 1184 4056
Size: 1184 2872
AleI CACnn'nnGTG
Cuts at: 0 361 1278 2189 2996 4056
Size: 361 917 911 807 1060
Fragments arranged by size:
1060 917 911 807 361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1028 4056
Size: 1028 3028
AlwNI CAG_nnn'CTG
Cuts at: 0 585 933 1572 1967 2754 3394 4056
Size: 585 348 639 395 787 640 662
Fragments arranged by size:
787 662 640 639 585 395 348
ApaI G_GGCC'C
Cuts at: 0 184 1030 1865 3277 3757 4056
Size: 184 846 835 1412 480 299
Fragments arranged by size:
1412 846 835 480 299 184
ApaLI G'TGCA_C
Cuts at: 0 366 1720 4056
Size: 366 1354 2336
Fragments arranged by size:
2336 1354 366
ApoI r'AATT_y
Cuts at: 0 1592 2531 3214 4056
Size: 1592 939 683 842
Fragments arranged by size:
1592 939 842 683
AvaI C'yCGr_G
Cuts at: 0 152 406 857 1144 1307 1838 3074 3399
Size: 152 254 451 287 163 531 1236 325
Cuts at: 3399 3746 3871 4056
Size: 347 125 185
Fragments arranged by size:
1236 531 451 347 325 287 254 185
163 152 125
BamHI G'GATC_C
Cuts at: 0 1216 2306 2429 2507 3065 4056
Size: 1216 1090 123 78 558 991
Fragments arranged by size:
1216 1090 991 558 123 78
BanI G'GyrC_C
Cuts at: 0 601 1165 1606 1728 2940 3363 3428 3516
Size: 601 564 441 122 1212 423 65 88
Cuts at: 3516 3781 3856 4056
Size: 265 75 200
Fragments arranged by size:
1212 601 564 441 423 265 200 122
88 75 65
BanII G_rGCy'C
Cuts at: 0 150 184 323 895 1030 1865 1940 2626
Size: 150 34 139 572 135 835 75 686
Cuts at: 2626 2746 3277 3485 3757 3987 4056
Size: 120 531 208 272 230 69
Fragments arranged by size:
835 686 572 531 272 230 208 150
139 135 120 75 69 34
BbeI G_GCGC'C
Cuts at: 0 1169 1610 4056
Size: 1169 441 2446
Fragments arranged by size:
2446 1169 441
BbsI GAAGACnn'nnnn_
Cuts at: 0 1554 4056
Size: 1554 2502
BbvCI CC'TCA_GC
Cuts at: 0 1555 2449 3350 3905 4056
Size: 1555 894 901 555 151
Fragments arranged by size:
1555 901 894 555 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1829 1863 4056
Size: 1829 34 2193
Fragments arranged by size:
2193 1829 34
BclI T'GATC_A
Cuts at: 0 3149 4056
Size: 3149 907
BglI GCCn_nnn'nGGC
Cuts at: 0 154 2648 4056
Size: 154 2494 1408
Fragments arranged by size:
2494 1408 154
BglII A'GATC_T
Cuts at: 0 731 1397 4056
Size: 731 666 2659
Fragments arranged by size:
2659 731 666
Bme1580I G_kGCm'C
Cuts at: 0 184 370 966 1030 1308 1724 1733 1847
Size: 184 186 596 64 278 416 9 114
Cuts at: 1847 1865 2055 2945 3277 3757 4056
Size: 18 190 890 332 480 299
Fragments arranged by size:
890 596 480 416 332 299 278 190
186 184 114 64 18 9
BmgBI CAC'GTC
Cuts at: 0 1212 4047 4056
Size: 1212 2835 9
Fragments arranged by size:
2835 1212 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1681 1863 3966 4056
Size: 1681 182 2103 90
Fragments arranged by size:
2103 1681 182 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 2818 2983 3337 3499 4056
Size: 1450 1368 165 354 162 557
Fragments arranged by size:
1450 1368 557 354 165 162
Bpu10I CC'TnA_GC
Cuts at: 0 1555 2449 3350 3905 4056
Size: 1555 894 901 555 151
Fragments arranged by size:
1555 901 894 555 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 4056
Size: 99 3957
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 644 3512 4056
Size: 403 241 2868 544
Fragments arranged by size:
2868 544 403 241
BsaAI yAC'GTr
Cuts at: 0 540 2607 4056
Size: 540 2067 1449
Fragments arranged by size:
2067 1449 540
BsaBI GATnn'nnATC
Cuts at: 0 2311 2428 4056
Size: 2311 117 1628
Fragments arranged by size:
2311 1628 117
BsaHI Gr'CG_yC
Cuts at: 0 1166 1607 2245 2736 4056
Size: 1166 441 638 491 1320
Fragments arranged by size:
1320 1166 638 491 441
BsaWI w'CCGG_w
Cuts at: 0 130 2025 2336 4056
Size: 130 1895 311 1720
Fragments arranged by size:
1895 1720 311 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 1432 1462 3000 3030 3193 3223
Size: 266 30 1136 30 1538 30 163 30
Cuts at: 3223 3441 3471 4056
Size: 218 30 585
Fragments arranged by size:
1538 1136 585 266 218 163 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 79 82 313 316 2161 3367 3508
Size: 70 9 3 231 3 1845 1206 141
Cuts at: 3508 3908 4056
Size: 400 148
Fragments arranged by size:
1845 1206 400 231 148 141 70 9
3 3
BseYI C'CCAG_C
Cuts at: 0 20 243 314 605 767 902 1095 1443
Size: 20 223 71 291 162 135 193 348
Cuts at: 1443 1632 1647 1850 2092 3069 3116 3239 3276
Size: 189 15 203 242 977 47 123 37
Cuts at: 3276 3368 3406 3607 3695 3731 3933 3991 4056
Size: 92 38 201 88 36 202 58 65
Fragments arranged by size:
977 348 291 242 223 203 202 201
193 189 162 135 123 92 88 71
65 58 47 38 37 36 20 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 550 1005 1072 1206 1225 1311 1582
Size: 478 72 455 67 134 19 86 271
Cuts at: 1582 2163 2692 3114 4056
Size: 581 529 422 942
Fragments arranged by size:
942 581 529 478 455 422 271 134
86 72 67 19
BsiHKAI G_wGCw'C
Cuts at: 0 289 358 370 545 1337 1724 4056
Size: 289 69 12 175 792 387 2332
Fragments arranged by size:
2332 792 387 289 175 69 12
BsmI GAATG_Cn'
Cuts at: 0 1924 1962 2270 4056
Size: 1924 38 308 1786
Fragments arranged by size:
1924 1786 308 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2434 4056
Size: 2434 1622
Bsp1286I G_dGCh'C
Cuts at: 0 150 184 289 323 358 370 545 895
Size: 150 34 105 34 35 12 175 350
Cuts at: 895 966 1030 1308 1337 1724 1733 1847 1865
Size: 71 64 278 29 387 9 114 18
Cuts at: 1865 1940 2055 2626 2746 2945 3277 3485 3757
Size: 75 115 571 120 199 332 208 272
Cuts at: 3757 3987 4056
Size: 230 69
Fragments arranged by size:
571 387 350 332 278 272 230 208
199 175 150 120 115 114 105 75
71 69 64 35 34 34 29 18
12 9
BspEI T'CCGG_A
Cuts at: 0 2336 4056
Size: 2336 1720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 491 558 1580 2762 3098 3694 3783 4056
Size: 491 67 1022 1182 336 596 89 273
Fragments arranged by size:
1182 1022 596 491 336 273 89 67
BsrBI CCG'CTC
Cuts at: 0 854 1464 2833 3108 3308 3978 4056
Size: 854 610 1369 275 200 670 78
Fragments arranged by size:
1369 854 670 610 275 200 78
BsrFI r'CCGG_y
Cuts at: 0 370 952 1015 1151 1784 2978 4056
Size: 370 582 63 136 633 1194 1078
Fragments arranged by size:
1194 1078 633 582 370 136 63
BssHII G'CGCG_C
Cuts at: 0 27 163 197 4056
Size: 27 136 34 3859
Fragments arranged by size:
3859 136 34 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1728 1971 4056
Size: 585 1143 243 2085
Fragments arranged by size:
2085 1143 585 243
BstBI TT'CG_AA
Cuts at: 0 717 4056
Size: 717 3339
BstEII G'GTnAC_C
Cuts at: 0 1104 1513 3553 4056
Size: 1104 409 2040 503
Fragments arranged by size:
2040 1104 503 409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 2929 3288 4056
Size: 297 2632 359 768
Fragments arranged by size:
2632 768 359 297
BstYI r'GATC_y
Cuts at: 0 731 1216 1397 2306 2333 2429 2470 2507
Size: 731 485 181 909 27 96 41 37
Cuts at: 2507 3065 3080 4056
Size: 558 15 976
Fragments arranged by size:
976 909 731 558 485 181 96 41
37 27 15
BstZ17I GTA'TAC
Cuts at: 0 1157 4056
Size: 1157 2899
BtgI C'CryG_G
Cuts at: 0 175 264 290 2666 2730 3860 4056
Size: 175 89 26 2376 64 1130 196
Fragments arranged by size:
2376 1130 196 175 89 64 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 531 2419 4056
Size: 531 1888 1637
Fragments arranged by size:
1888 1637 531
BtsI GCAGTG_nn'
Cuts at: 0 287 574 979 1012 1089 1454 1683 1775
Size: 287 287 405 33 77 365 229 92
Cuts at: 1775 1845 3310 4056
Size: 70 1465 746
Fragments arranged by size:
1465 746 405 365 287 287 229 92
77 70 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1053 1088 4056
Size: 1053 35 2968
Fragments arranged by size:
2968 1053 35
DraIII CAC_nnn'GTG
Cuts at: 0 1728 1871 4056
Size: 1728 143 2185
Fragments arranged by size:
2185 1728 143
DrdI GACnn_nn'nnGTC
Cuts at: 0 1558 4056
Size: 1558 2498
EaeI y'GGCC_r
Cuts at: 0 632 1534 1786 2540 2828 3187 3227 4056
Size: 632 902 252 754 288 359 40 829
Fragments arranged by size:
902 829 754 632 359 288 252 40
EarI CTCTTCn'nnn_
Cuts at: 0 1496 3485 4056
Size: 1496 1989 571
Fragments arranged by size:
1989 1496 571
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 2965 4056
Size: 418 2547 1091
Fragments arranged by size:
2547 1091 418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1450 2818 2983 3337 3499 3509 3912 4056
Size: 1450 1368 165 354 162 10 403 144
Fragments arranged by size:
1450 1368 403 354 165 162 144 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 677 4056
Size: 439 238 3379
Fragments arranged by size:
3379 439 238
EcoO109I rG'GnC_Cy
Cuts at: 0 180 181 570 683 1027 1440 1812 1861
Size: 180 1 389 113 344 413 372 49
Cuts at: 1861 2064 2751 3234 3336 3462 3498 3633 3753
Size: 203 687 483 102 126 36 135 120
Cuts at: 3753 3754 3966 4056
Size: 1 212 90
Fragments arranged by size:
687 483 413 389 372 344 212 203
180 135 126 120 113 102 90 49
36 1 1
EcoRI G'AATT_C
Cuts at: 0 1592 3214 4056
Size: 1592 1622 842
Fragments arranged by size:
1622 1592 842
EcoRV GAT'ATC
Cuts at: 0 519 4056
Size: 519 3537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 1416 4056
Size: 822 594 2640
Fragments arranged by size:
2640 822 594
HaeII r_GCGC'y
Cuts at: 0 1169 1610 2480 2962 3246 3407 4056
Size: 1169 441 870 482 284 161 649
Fragments arranged by size:
1169 870 649 482 441 284 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 1201 1202 1233 1432 1464 1673 1705
Size: 1170 31 1 31 199 32 209 32
Cuts at: 1705 3193 3225 4056
Size: 1488 32 831
Fragments arranged by size:
1488 1170 831 209 199 32 32 32
31 31 1
HincII GTy'rAC
Cuts at: 0 1878 4056
Size: 1878 2178
KasI G'GCGC_C
Cuts at: 0 1165 1606 4056
Size: 1165 441 2450
Fragments arranged by size:
2450 1165 441
KpnI G_GTAC'C
Cuts at: 0 3367 4056
Size: 3367 689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 3145 4056
Size: 1475 1670 911
Fragments arranged by size:
1670 1475 911
MscI TGG'CCA
Cuts at: 0 2542 3229 4056
Size: 2542 687 827
Fragments arranged by size:
2542 827 687
MslI CAynn'nnrTG
Cuts at: 0 361 379 1278 2189 2548 2927 2996 3151
Size: 361 18 899 911 359 379 69 155
Cuts at: 3151 4056
Size: 905
Fragments arranged by size:
911 905 899 379 361 359 155 69
18
MspA1I CmG'CkG
Cuts at: 0 314 657 759 825 899 968 1172 1278
Size: 314 343 102 66 74 69 204 106
Cuts at: 1278 1643 1830 2087 2096 2388 2676 2732 3621
Size: 365 187 257 9 292 288 56 889
Cuts at: 3621 4056
Size: 435
Fragments arranged by size:
889 435 365 343 314 292 288 257
204 187 106 102 74 69 66 56
9
NaeI GCC'GGC
Cuts at: 0 1017 2980 4056
Size: 1017 1963 1076
Fragments arranged by size:
1963 1076 1017
NarI GG'CG_CC
Cuts at: 0 1166 1607 4056
Size: 1166 441 2449
Fragments arranged by size:
2449 1166 441
NcoI C'CATG_G
Cuts at: 0 264 290 4056
Size: 264 26 3766
Fragments arranged by size:
3766 264 26
NdeI CA'TA_TG
Cuts at: 0 2631 4056
Size: 2631 1425
NgoMIV G'CCGG_C
Cuts at: 0 1015 2978 4056
Size: 1015 1963 1078
Fragments arranged by size:
1963 1078 1015
NspI r_CATG'y
Cuts at: 0 426 2124 2159 4056
Size: 426 1698 35 1897
Fragments arranged by size:
1897 1698 426 35
PasI CC'CwG_GG
Cuts at: 0 1733 1943 3814 3888 4056
Size: 1733 210 1871 74 168
Fragments arranged by size:
1871 1733 210 168 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 2043 2951 4056
Size: 2043 908 1105
Fragments arranged by size:
2043 1105 908
PfoI T'CCnGG_A
Cuts at: 0 521 1373 2720 3197 4056
Size: 521 852 1347 477 859
Fragments arranged by size:
1347 859 852 521 477
PpuMI rG'GwC_Cy
Cuts at: 0 570 1440 2064 2751 3234 3336 3462 3633
Size: 570 870 624 687 483 102 126 171
Cuts at: 3633 3966 4056
Size: 333 90
Fragments arranged by size:
870 687 624 570 483 333 171 126
102 90
PspOMI G'GGCC_C
Cuts at: 0 180 1026 1861 3273 3753 4056
Size: 180 846 835 1412 480 303
Fragments arranged by size:
1412 846 835 480 303 180
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3859 3891 4015 4047 4056
Size: 3859 32 124 32 9
Fragments arranged by size:
3859 124 32 32 9
PstI C_TGCA'G
Cuts at: 0 515 581 713 1885 1934 2366 3691 4056
Size: 515 66 132 1172 49 432 1325 365
Fragments arranged by size:
1325 1172 515 432 365 132 66 49
PvuII CAG'CTG
Cuts at: 0 825 1172 1643 1830 2676 4056
Size: 825 347 471 187 846 1380
Fragments arranged by size:
1380 846 825 471 347 187
SacII CC_GC'GG
Cuts at: 0 2733 4056
Size: 2733 1323
SanDI GG'GwC_CC
Cuts at: 0 1440 3234 4056
Size: 1440 1794 822
Fragments arranged by size:
1794 1440 822
SbfI CC_TGCA'GG
Cuts at: 0 515 4056
Size: 515 3541
ScaI AGT'ACT
Cuts at: 0 1928 3877 4056
Size: 1928 1949 179
Fragments arranged by size:
1949 1928 179
SexAI A'CCwGG_T
Cuts at: 0 3338 4056
Size: 3338 718
SfcI C'TryA_G
Cuts at: 0 511 577 709 1881 1930 2362 3616 3687
Size: 511 66 132 1172 49 432 1254 71
Cuts at: 3687 4056
Size: 369
Fragments arranged by size:
1254 1172 511 432 369 132 71 66
49
SfoI GGC'GCC
Cuts at: 0 1167 1608 4056
Size: 1167 441 2448
Fragments arranged by size:
2448 1167 441
SgrAI Cr'CCGG_yG
Cuts at: 0 2978 4056
Size: 2978 1078
SmaI CCC'GGG
Cuts at: 0 154 3076 3401 4056
Size: 154 2922 325 655
Fragments arranged by size:
2922 655 325 154
SmlI C'TyrA_G
Cuts at: 0 78 4056
Size: 78 3978
SphI G_CATG'C
Cuts at: 0 2159 4056
Size: 2159 1897
StuI AGG'CCT
Cuts at: 0 698 4056
Size: 698 3358
StyI C'CwwG_G
Cuts at: 0 264 290 2780 3287 3575 4056
Size: 264 26 2490 507 288 481
Fragments arranged by size:
2490 507 481 288 264 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 619 1720 1882 2197 3004 4005 4056
Size: 373 246 1101 162 315 807 1001 51
Fragments arranged by size:
1101 1001 807 373 315 246 162 51
TatI w'GTAC_w
Cuts at: 0 1926 2342 3875 4056
Size: 1926 416 1533 181
Fragments arranged by size:
1926 1533 416 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 3000 3032 3441 3473 4056
Size: 266 32 2702 32 409 32 583
Fragments arranged by size:
2702 583 409 266 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2716 4056
Size: 2716 1340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1381 2673 2952 3510 4056
Size: 1381 1292 279 558 546
Fragments arranged by size:
1381 1292 558 546 279
XmaI C'CCGG_G
Cuts at: 0 152 3074 3399 4056
Size: 152 2922 325 657
Fragments arranged by size:
2922 657 325 152
ZraI GAC'GTC
Cuts at: 0 2246 4056
Size: 2246 1810
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacII SanDI SbfI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AloI AscI AseI AsiSI
AvrII BaeI BciVI BfrBI BlpI BmtI BplI BsiEI
BsiWI BspHI BsrDI BsrGI BssSI Bsu36I ClaI DraI
EagI EcoICRI FseI FspI FspAI HindIII HpaI MfeI
MluI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacI
SalI SapI SfiI SgfI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmnI