(Circular) (Six-base) MAPSORT of: pFN21AE1088.seq Check: 6171 from: 1 to: 7423 pFN21AE1088 7423 bp With 182 enzymes: SgfI * October 14, 2009 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2506 3022 2506 Size: 516 6907 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5233 278 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2442 4766 1937 Size: 505 2324 4594 Fragments arranged by size: 4594 2324 505 Acc65I G'GTAC_C Cuts at: 4744 4744 Size: 7423 AclI AA'CG_TT Cuts at: 1563 5550 5923 1563 Size: 3987 373 3063 Fragments arranged by size: 3987 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3667 4006 5486 6870 7138 1632 Size: 725 1310 339 1480 1384 268 1917 Fragments arranged by size: 1917 1480 1384 1310 725 339 268 AfeI AGC'GCT Cuts at: 4402 4402 Size: 7423 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7226 AflIII A'CryG_T Cuts at: 1203 6322 7299 1203 Size: 5119 977 1327 Fragments arranged by size: 5119 1327 977 AgeI A'CCGG_T Cuts at: 2033 6254 2033 Size: 4221 3202 AhdI GACnn_n'nnGTC Cuts at: 6152 6152 Size: 7423 AleI CACnn'nnGTG Cuts at: 1166 2843 1166 Size: 1677 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2034 1184 Size: 850 6573 AlwNI CAG_nnn'CTG Cuts at: 2501 2578 2923 5166 6738 2501 Size: 77 345 2243 1572 3186 Fragments arranged by size: 3186 2243 1572 345 77 ApaI G_GGCC'C Cuts at: 2314 2314 Size: 7423 ApaLI G'TGCA_C Cuts at: 5480 6636 5480 Size: 1156 6267 ApoI r'AATT_y Cuts at: 1493 2435 2729 4732 4958 5110 7219 1493 Size: 942 294 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 942 294 226 152 AseI AT'TA_AT Cuts at: 160 1032 5977 160 Size: 872 4945 1606 Fragments arranged by size: 4945 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 AvaI C'yCGr_G Cuts at: 1870 1943 2829 3402 4748 1870 Size: 73 886 573 1346 4545 Fragments arranged by size: 4545 1346 886 573 73 AvrII C'CTAG_G Cuts at: 2041 2041 Size: 7423 BamHI G'GATC_C Cuts at: 4753 5120 4753 Size: 367 7056 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2744 4744 6199 618 Size: 325 217 627 270 687 2000 1455 1842 Fragments arranged by size: 2000 1842 1455 687 627 325 270 217 BanII G_rGCy'C Cuts at: 1391 2160 2314 3025 3403 4742 1391 Size: 769 154 711 378 1339 4072 Fragments arranged by size: 4072 1339 769 711 378 154 BbeI G_GCGC'C Cuts at: 2748 2748 Size: 7423 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3580 928 Size: 971 1681 4771 Fragments arranged by size: 4771 1681 971 BbvCI CC'TCA_GC Cuts at: 2907 4412 2907 Size: 1505 5918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5614 5648 1903 Size: 34 50 34 3593 34 3678 Fragments arranged by size: 3678 3593 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2485 2549 5315 6525 2485 Size: 64 2766 1210 3383 Fragments arranged by size: 3383 2766 1210 64 BclI T'GATC_A Cuts at: 1548 1548 Size: 7423 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3905 6034 136 Size: 107 122 71 631 605 2233 2129 1525 Fragments arranged by size: 2233 2129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7418 7418 Size: 7423 BlpI GC'TnA_GC Cuts at: 4840 4840 Size: 7423 Bme1580I G_kGCm'C Cuts at: 1790 2314 5484 6640 1790 Size: 524 3170 1156 2573 Fragments arranged by size: 3170 2573 1156 524 BmgBI CAC'GTC Cuts at: 1318 2320 1318 Size: 1002 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2227 5154 6112 Size: 416 53 83 399 24 810 2927 958 Cuts at: 6112 442 Size: 1753 Fragments arranged by size: 2927 1753 958 810 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 3392 4178 6083 1239 Size: 1361 792 786 1905 2579 Fragments arranged by size: 2579 1905 1361 792 786 Bpu10I CC'TnA_GC Cuts at: 2907 4412 2907 Size: 1505 5918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3254 5548 6413 6711 3254 Size: 2294 865 298 3966 Fragments arranged by size: 3966 2294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2826 2851 3399 6086 882 Size: 617 1327 25 548 2687 2219 Fragments arranged by size: 2687 2219 1327 617 548 25 BsaAI yAC'GTr Cuts at: 493 1204 3106 3352 5214 493 Size: 711 1902 246 1862 2702 Fragments arranged by size: 2702 1902 1862 711 246 BsaBI GATnn'nnATC Cuts at: 3011 3548 7417 3011 Size: 537 3869 3017 Fragments arranged by size: 3869 3017 537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2745 3082 3855 4317 Size: 53 83 186 936 1212 337 773 462 Cuts at: 4317 5230 5612 6247 275 Size: 913 382 635 1451 Fragments arranged by size: 1451 1212 936 913 773 635 462 382 337 186 83 53 BsaWI w'CCGG_w Cuts at: 2033 2559 4379 5856 6254 6528 6675 7086 2033 Size: 526 1820 1477 398 274 147 411 2370 Fragments arranged by size: 2370 1820 1477 526 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3432 3462 3642 3672 1178 Size: 30 165 30 2029 30 180 30 4929 Fragments arranged by size: 4929 2029 180 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 3877 4121 1186 Size: 1378 1313 244 4488 Fragments arranged by size: 4488 1378 1313 244 BseYI C'CCAG_C Cuts at: 2963 2983 3194 6626 7122 2963 Size: 20 211 3432 496 3264 Fragments arranged by size: 3432 3264 496 211 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 3083 3972 1163 Size: 573 278 1069 889 4614 Fragments arranged by size: 4614 1069 889 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2461 4873 5634 6662 1540 Size: 275 186 460 2412 761 1028 2301 Fragments arranged by size: 2412 2301 1028 761 460 275 186 BsiHKAI G_wGCw'C Cuts at: 3025 5484 5569 6640 3025 Size: 2459 85 1071 3808 Fragments arranged by size: 3808 2459 1071 85 BsmI GAATG_Cn' Cuts at: 2965 4934 5027 2965 Size: 1969 93 5361 Fragments arranged by size: 5361 1969 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2314 3025 3403 4742 5484 5569 Size: 399 370 154 711 378 1339 742 85 Cuts at: 5569 6640 1391 Size: 1071 2174 Fragments arranged by size: 2174 1339 1071 742 711 399 378 370 154 85 BspEI T'CCGG_A Cuts at: 4379 7086 4379 Size: 2707 4716 BspHI T'CATG_A Cuts at: 3552 5312 7042 3552 Size: 1760 1730 3933 Fragments arranged by size: 3933 1760 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2506 3022 4778 844 Size: 1662 516 1756 3489 Fragments arranged by size: 3489 1756 1662 516 BsrBI CCG'CTC Cuts at: 1394 1823 2596 5310 1394 Size: 429 773 2714 3507 Fragments arranged by size: 3507 2714 773 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3304 4245 4423 5918 6092 65 Size: 1183 2056 941 178 1495 174 1396 Fragments arranged by size: 2056 1495 1396 1183 941 178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 6067 6254 1700 Size: 110 223 4034 187 2869 Fragments arranged by size: 4034 2869 223 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7423 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7423 BssSI C'ACGA_G Cuts at: 5483 6495 5483 Size: 1012 6411 BstAPI GCAn_nnn'nTGC Cuts at: 794 3034 3341 794 Size: 2240 307 4876 Fragments arranged by size: 4876 2240 307 BstBI TT'CG_AA Cuts at: 6227 6227 Size: 7423 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7423 BstXI CCAn_nnnn'nTGG Cuts at: 3352 4213 4720 3352 Size: 861 507 6055 Fragments arranged by size: 6055 861 507 BstYI r'GATC_y Cuts at: 1972 3253 3543 4004 4753 5120 5505 5522 6974 Size: 1281 290 461 749 367 385 17 1452 Cuts at: 6974 7060 7072 7418 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1281 749 461 385 367 346 290 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3921 4713 513 Size: 552 2856 792 3223 Fragments arranged by size: 3223 2856 792 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6684 Fragments arranged by size: 6684 683 56 BtsI GCAGTG_nn' Cuts at: 2816 4624 4939 5733 5753 2816 Size: 1808 315 794 20 4486 Fragments arranged by size: 4486 1808 794 315 20 ClaI AT'CG_AT Cuts at: 1554 4670 1554 Size: 3116 4307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2722 2757 529 Size: 35 2158 35 5195 Fragments arranged by size: 5195 2158 35 35 DraI TTT'AAA Cuts at: 4727 4727 Size: 7423 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6430 809 Size: 507 5114 1802 Fragments arranged by size: 5114 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2793 3906 4870 Size: 54 1425 50 275 18 963 1113 964 Cuts at: 4870 5759 7388 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1113 964 963 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4870 1537 Size: 275 3058 4090 Fragments arranged by size: 4090 3058 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2849 3451 3671 3702 5353 1353 Size: 1496 602 220 31 1651 3423 Fragments arranged by size: 3423 1651 1496 602 220 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6010 6384 6530 387 Size: 1064 138 4421 374 146 1280 Fragments arranged by size: 4421 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3023 3023 Size: 7423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2600 3392 3667 4006 4178 5486 Size: 393 725 243 792 275 339 172 1308 Cuts at: 5486 6083 6870 7138 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1308 792 787 725 597 393 339 275 268 243 172 EcoO109I rG'GnC_Cy Cuts at: 2310 2311 2575 3828 3958 4277 2310 Size: 1 264 1253 130 319 5456 Fragments arranged by size: 5456 1253 319 264 130 1 EcoRI G'AATT_C Cuts at: 4732 5110 4732 Size: 378 7045 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4036 4036 Size: 7423 FspI TGC'GCA Cuts at: 5929 5929 Size: 7423 HaeII r_GCGC'y Cuts at: 1678 2748 4404 6570 1678 Size: 1070 1656 2166 2531 Fragments arranged by size: 2531 2166 1656 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2603 2635 3432 3464 3922 Size: 32 135 32 1031 32 797 32 458 Cuts at: 3922 3954 4387 4419 6064 6096 6138 6170 1373 Size: 32 433 32 1645 32 42 32 2626 Fragments arranged by size: 2626 1645 1031 797 458 433 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 2530 4767 5013 669 Size: 988 281 349 243 2237 246 3079 Fragments arranged by size: 3079 2237 988 349 281 246 243 HindIII A'AGCT_T Cuts at: 748 748 Size: 7423 HpaI GTT'AAC Cuts at: 5013 5013 Size: 7423 KasI G'GCGC_C Cuts at: 2744 2744 Size: 7423 KpnI G_GTAC'C Cuts at: 4748 4748 Size: 7423 MfeI C'AATT_G Cuts at: 5022 5022 Size: 7423 MluI A'CGCG_T Cuts at: 7299 7299 Size: 7423 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6537 6721 1305 Size: 5232 184 2007 Fragments arranged by size: 5232 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5601 Fragments arranged by size: 5601 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 3650 4211 5381 5740 5899 518 Size: 648 1677 807 561 1170 359 159 2042 Fragments arranged by size: 2042 1677 1170 807 648 561 359 159 MspA1I CmG'CkG Cuts at: 1606 2377 2498 2767 2987 4189 4593 4611 4839 Size: 771 121 269 220 1202 404 18 228 Cuts at: 4839 5516 6664 6909 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1202 1148 771 677 404 269 245 228 220 121 18 NarI GG'CG_CC Cuts at: 2745 2745 Size: 7423 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6871 NdeI CA'TA_TG Cuts at: 387 387 Size: 7423 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7423 NotI GC'GGCC_GC Cuts at: 4870 4870 Size: 7423 NspI r_CATG'y Cuts at: 1132 1229 2123 2293 4781 6326 1132 Size: 97 894 170 2488 1545 2229 Fragments arranged by size: 2488 2229 1545 894 170 97 PasI CC'CwG_GG Cuts at: 1348 2994 3787 1348 Size: 1646 793 4984 Fragments arranged by size: 4984 1646 793 PciI A'CATG_T Cuts at: 6322 6322 Size: 7423 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7423 PfoI T'CCnGG_A Cuts at: 2487 3498 4155 2487 Size: 1011 657 5755 Fragments arranged by size: 5755 1011 657 PmeI GTTT'AAAC Cuts at: 4727 4727 Size: 7423 PmlI CAC'GTG Cuts at: 3106 3352 3106 Size: 246 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4675 4707 5492 5524 7005 7037 4675 Size: 32 785 32 1481 32 5061 Fragments arranged by size: 5061 1481 785 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2575 3828 3958 4277 2575 Size: 1253 130 319 5721 Fragments arranged by size: 5721 1253 319 130 PshAI GACnn'nnGTC Cuts at: 1613 2525 1613 Size: 912 6511 PsiI TTA'TAA Cuts at: 2654 4993 2654 Size: 2339 5084 PspOMI G'GGCC_C Cuts at: 2310 2310 Size: 7423 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3212 3244 3212 Size: 32 7391 PstI C_TGCA'G Cuts at: 830 2394 2481 3030 3178 4775 830 Size: 1564 87 549 148 1597 3478 Fragments arranged by size: 3478 1597 1564 549 148 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7423 PvuII CAG'CTG Cuts at: 2377 2498 2767 2987 2377 Size: 121 269 220 6813 Fragments arranged by size: 6813 269 220 121 SacI G_AGCT'C Cuts at: 3025 3025 Size: 7423 SalI G'TCGA_C Cuts at: 1936 4765 1936 Size: 2829 4594 SapI GCTCTTCn'nnn_ Cuts at: 3451 3451 Size: 7423 SbfI CC_TGCA'GG Cuts at: 4775 4775 Size: 7423 ScaI AGT'ACT Cuts at: 5671 7383 5671 Size: 1712 5711 SfcI C'TryA_G Cuts at: 826 1045 2218 2390 2477 3026 3174 4771 5906 Size: 219 1173 172 87 549 148 1597 1135 Cuts at: 5906 6587 6778 826 Size: 681 191 1471 Fragments arranged by size: 1597 1471 1173 1135 681 549 219 191 172 148 87 SfoI GGC'GCC Cuts at: 2746 2746 Size: 7423 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7423 SmaI CCC'GGG Cuts at: 1872 4750 1872 Size: 2878 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2829 3269 5527 6428 6690 820 Size: 197 926 886 440 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 886 440 262 197 SnaBI TAC'GTA Cuts at: 493 5214 493 Size: 4721 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7423 SphI G_CATG'C Cuts at: 1132 4781 1132 Size: 3649 3774 SspI AAT'ATT Cuts at: 5 52 5347 5 Size: 47 5295 2081 Fragments arranged by size: 5295 2081 47 StuI AGG'CCT Cuts at: 1517 3422 1517 Size: 1905 5518 StyI C'CwwG_G Cuts at: 513 1065 2041 3001 4257 4704 4862 513 Size: 552 976 960 1256 447 158 3074 Fragments arranged by size: 3074 1256 976 960 552 447 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4270 5450 5467 5620 5805 6250 325 Size: 1255 2690 1180 17 153 185 445 1498 Fragments arranged by size: 2690 1498 1255 1180 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2865 4176 5669 Size: 274 80 33 51 1443 888 1311 1493 Cuts at: 5669 7381 96 Size: 1712 138 Fragments arranged by size: 1712 1493 1443 1311 888 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2806 2838 4183 4215 7383 7415 2806 Size: 32 1345 32 3168 32 2814 Fragments arranged by size: 3168 2814 1345 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7423 XbaI T'CTAG_A Cuts at: 4759 4759 Size: 7423 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2991 2991 Size: 7423 XhoI C'TCGA_G Cuts at: 1943 2829 1943 Size: 886 6537 XmaI C'CCGG_G Cuts at: 1870 4748 1870 Size: 2878 4545 XmnI GAAnn'nnTTC Cuts at: 5552 5552 Size: 7423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5231 276 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI