Restriction map for
pFN21AE1010
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoRV EcoT14I FbaI FseI FspI
HgaI Hin1I HinfI HpaI HphI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvaI MvnI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AluI 563 AGCT AG^CT
ApaI 118 GGGCCC GGGCC^C
AvaI 218 CTCGGG C^[CT]CG[GA]G
BanI 277 GGTGCC G^G[CT][GA]CC
BanII 118 GGGCCC G[GA]GC[CT]^C
BcgI 76 GCAACGTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 414 CCCGG CC^[GC]GG
BfaI 605 CTAG C^TAG
BmyI 118 GGGCCC G[AGT]GC[ACT]^C
BpmI 49 CTGGAG CTGGAG(16/14)
BsaJI 217 CCTCGG C^C[ACGT][ACGT]GG
BsmI 390 GAATGC GAATGC(1/-1)
BsoBI 218 CTCGGG C^[CT]CG[GA]G
Bsp1286I 118 GGGCCC G[AGT]GC[ACT]^C
BsrFI 280 GCCGGC [GA]^CCGG[CT]
BssKI 414 CCCGG ^CC[ACGT]GG
CfoI 205 GCGC GCG^C
Cfr10I 280 GCCGGC [GA]^CCGG[CT]
DdeI 587 CTAAG C^T[ACGT]AG
DpnII 515 GATC ^GATC
DraI 367 TTTAAA TTT^AAA
EcoRI 637 GAATTC G^AATTC
EcoT22I 392 ATGCAT ATGCA^T
HaeII 204 AGCGCC [GA]GCGC^[CT]
HhaI 205 GCGC GCG^C
HinP1I 205 GCGC G^CGC
HincII 582 GTTGAC GT[CT]^[GA]AC
HindII 582 GTTGAC GT[CT]^[GA]AC
HindIII 562 AAGCTT A^AGCTT
MaeI 605 CTAG C^TAG
MaeII 79 ACGT A^CGT
MboI 515 GATC ^GATC
MslI 288 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 280 GCCGGC GCC^GGC
NciI 414 CCCGG CC^[GC]GG
NdeII 515 GATC ^GATC
NgoMIV 280 GCCGGC G^CCGGC
NsiI 392 ATGCAT ATGCA^T
NspI 394 GCATGT [GA]CATG^[CT]
Sau3AI 515 GATC ^GATC
ScrFI 414 CCCGG CC^[ACGT]GG
SmlI 303 CTTGAG C^T[CT][GA]AG
TaqI 32 TCGA T^CGA
TthHB8I 32 TCGA T^CGA
XcmI 38 CCATCTCGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsiYI 212 CCCCGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
213 CCCGCCTCGGG
BslI 212 CCCCGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
213 CCCGCCTCGGG
BsmAI 446 GTCTC GTCTC(1/5)
494 GTCTC
BstF5I 6 CATCC GGATG(2/0)
138 GGATG
Cac8I 280 GCCGGC GC[ACGT]^[ACGT]GC
564 GCTTGC
EaeI 282 CGGCCA [CT]^GGCC[GA]
381 TGGCCG
FauI 213 CCCGC CCCGC(4/6)
273 GCGGG
FokI 6 CATCC GGATG(9/13)
138 GGATG
HapII 281 CCGG C^CGG
415 CCGG
HpaII 281 CCGG C^CGG
415 CCGG
MseI 368 TTAA T^TAA
529 TTAA
MspI 281 CCGG C^CGG
415 CCGG
NlaIII 21 CATG CATG^
395 CATG
SfaNI 5 GCATC GCATC(5/9)
237 GCATC
Tru9I 368 TTAA T^TAA
529 TTAA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AcsI ApoI BbvI BseRI
BsrI Cfr13I EarI Fnu4HI HaeIII
ItaI Ksp632I MboII MnlI MwoI
NlaIV Sau96I TseI Tsp509I TspRI