################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AlwNI ApaI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 317 GGCGTC G[GA]^CG[CT]C AhdI 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 800 GTGCAC G^TGCAC AlwI 1038 GGATC GGATC(4/5) AocI 874 CCTAAGG CC^T[ACGT]AGG Aor51HI 1253 AGCGCT AGC^GCT ApaLI 800 GTGCAC G^TGCAC AspEI 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 362 TGGCCA TGG^CCA BbsI 1290 GAAGAC GAAGAC(2/6) BbvCI 703 CCTCAGC CCTCAGC(-5/-2) BglI 984 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1290 GAAGAC GAAGAC(2/6) BsaHI 317 GGCGTC G[GA]^CG[CT]C BsmI 509 GCATTC GAATGC(1/-1) BsrFI 1196 ACCGGC [GA]^CCGG[CT] BstAPI 1200 GCACATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1038 GGATCT [GA]^GATC[CT] Bsu36I 874 CCTAAGG CC^T[ACGT]AGG Cfr10I 1196 ACCGGC [GA]^CCGG[CT] Eam1105I 673 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1253 AGCGCT AGC^GCT Eco81I 874 CCTAAGG CC^T[ACGT]AGG EcoRV 885 GATATC GAT^ATC FauI 31 CCCGC CCCGC(4/6) HaeII 1253 AGCGCT [GA]GCGC^[CT] HgaI 318 GCGTC GACGC(5/10) Hin1I 317 GGCGTC G[GA]^CG[CT]C HindIII 781 AAGCTT A^AGCTT HphI 793 GGTGA GGTGA(8/7) MaeIII 1193 GTGAC ^GT[ACGT]AC MflI 1038 GGATCT [GA]^GATC[CT] MluNI 362 TGGCCA TGG^CCA MscI 362 TGGCCA TGG^CCA MseI 854 TTAA T^TAA MslI 1045 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 1311 GCATGC [GA]CATG^[CT] PflMI 1000 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1133 CTGCAG CTGCA^G SmlI 808 CTTGAG C^T[CT][GA]AG SphI 1311 GCATGC GCATG^C TfiI 106 GAATC G^A[AT]TC Tru9I 854 TTAA T^TAA Tsp45I 1193 GTGAC ^GT[GC]AC Van91I 1000 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1092 CCAGGCCGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1038 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 28 GTAC GT^AC 538 GTAC BcnI 168 CCGGG CC^[GC]GG 1368 CCGGG BsgI 827 CTGCAC GTGCAG(16/14) 160 GTGCAG BsmAI 251 GTCTC GTCTC(1/5) 320 GTCTC BsmBI 250 CGTCTC CGTCTC(1/5) 319 CGTCTC BspMI 43 ACCTGC ACCTGC(4/8) 384 GCAGGT CfoI 244 GCGC GCG^C 1254 GCGC DraII 1074 AGGACCT [GA]G^G[ACGT]CC[CT] 1370 GGGACCT DsaI 58 CCACGG C^C[GA][CT]GG 450 CCGTGG EaeI 362 TGGCCA [CT]^GGCC[GA] 1329 TGGCCG EcoO109I 1074 AGGACCT [GA]G^G[ACGT]CC[CT] 1370 GGGACCT HhaI 244 GCGC GCG^C 1254 GCGC HinP1I 244 GCGC G^CGC 1254 GCGC NciI 168 CCGGG CC^[GC]GG 1368 CCGGG PleI 699 GACTC GAGTC(4/5) 1343 GAGTC PpuMI 1074 AGGACCT [GA]G^G[AT]CC[CT] 1370 GGGACCT RsaI 28 GTAC GT^AC 538 GTAC SapI 340 GCTCTTC GCTCTTC(1/4) 515 GCTCTTC SfcI 494 CTACAG C^T[GA][CT]AG 1133 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AcsI AluI ApoI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoNI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I