(Linear) (Six-base) MAPSORT of: KIEE1017.seq Check: 8898 from: 1 to: 1419 KIEE1017.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 413 971 974 1200 1285 1419 Size: 413 558 3 226 85 134 Fragments arranged by size: 558 413 226 134 85 3 AfeI AGC'GCT Cuts at: 0 1255 1419 Size: 1255 164 AhdI GACnn_n'nnGTC Cuts at: 0 678 1419 Size: 678 741 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 958 990 1419 Size: 958 32 429 Fragments arranged by size: 958 429 32 ApaLI G'TGCA_C Cuts at: 0 800 1419 Size: 800 619 ApoI r'AATT_y Cuts at: 0 407 440 623 1419 Size: 407 33 183 796 Fragments arranged by size: 796 407 183 33 BanII G_rGCy'C Cuts at: 0 183 583 1308 1419 Size: 183 400 725 111 Fragments arranged by size: 725 400 183 111 BbsI GAAGACnn'nnnn_ Cuts at: 0 1297 1419 Size: 1297 122 BbvCI CC'TCA_GC Cuts at: 0 704 1419 Size: 704 715 BglI GCCn_nnn'nGGC Cuts at: 0 990 1419 Size: 990 429 Bme1580I G_kGCm'C Cuts at: 0 804 1419 Size: 804 615 BmrI ACTGGGnnnn_n' Cuts at: 0 529 616 1419 Size: 529 87 803 Fragments arranged by size: 803 529 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 929 962 1322 1419 Size: 283 646 33 360 97 Fragments arranged by size: 646 360 283 97 33 Bpu10I CC'TnA_GC Cuts at: 0 704 1419 Size: 704 715 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 829 1419 Size: 829 590 BsaHI Gr'CG_yC Cuts at: 0 318 1419 Size: 318 1101 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 756 786 958 988 1419 Size: 756 30 172 30 431 Fragments arranged by size: 756 431 172 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 658 661 664 692 895 926 1419 Size: 658 3 3 28 203 31 493 Fragments arranged by size: 658 493 203 31 28 3 3 BseYI C'CCAG_C Cuts at: 0 51 1391 1419 Size: 51 1340 28 Fragments arranged by size: 1340 51 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 812 1419 Size: 181 631 607 Fragments arranged by size: 631 607 181 BsiHKAI G_wGCw'C Cuts at: 0 403 804 1286 1419 Size: 403 401 482 133 Fragments arranged by size: 482 403 401 133 BsmI GAATG_Cn' Cuts at: 0 509 1419 Size: 509 910 BsmBI CGTCTCn'nnnn_ Cuts at: 0 256 325 1419 Size: 256 69 1094 Fragments arranged by size: 1094 256 69 Bsp1286I G_dGCh'C Cuts at: 0 183 403 583 804 1286 1308 1419 Size: 183 220 180 221 482 22 111 Fragments arranged by size: 482 221 220 183 180 111 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 375 1419 Size: 52 323 1044 Fragments arranged by size: 1044 323 52 BsrFI r'CCGG_y Cuts at: 0 1196 1419 Size: 1196 223 BstAPI GCAn_nnn'nTGC Cuts at: 0 1206 1419 Size: 1206 213 BstYI r'GATC_y Cuts at: 0 1038 1419 Size: 1038 381 Bsu36I CC'TnA_GG Cuts at: 0 875 1419 Size: 875 544 BtgI C'CryG_G Cuts at: 0 58 450 1419 Size: 58 392 969 Fragments arranged by size: 969 392 58 BtsI GCAGTG_nn' Cuts at: 0 197 754 829 1184 1397 1419 Size: 197 557 75 355 213 22 Fragments arranged by size: 557 355 213 197 75 22 EaeI y'GGCC_r Cuts at: 0 362 1329 1419 Size: 362 967 90 Fragments arranged by size: 967 362 90 EarI CTCTTCn'nnn_ Cuts at: 0 347 522 635 1419 Size: 347 175 113 784 Fragments arranged by size: 784 347 175 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 313 1351 1419 Size: 313 1038 68 Fragments arranged by size: 1038 313 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 413 929 962 971 974 1200 1285 Size: 283 130 516 33 9 3 226 85 Cuts at: 1285 1322 1419 Size: 37 97 Fragments arranged by size: 516 283 226 130 97 85 37 33 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 160 587 1378 1419 Size: 160 427 791 41 Fragments arranged by size: 791 427 160 41 EcoO109I rG'GnC_Cy Cuts at: 0 1075 1371 1419 Size: 1075 296 48 Fragments arranged by size: 1075 296 48 EcoRV GAT'ATC Cuts at: 0 887 1419 Size: 887 532 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 584 1419 Size: 584 835 HaeII r_GCGC'y Cuts at: 0 1257 1419 Size: 1257 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 385 417 664 696 756 788 871 903 Size: 385 32 247 32 60 32 83 32 Cuts at: 903 1419 Size: 516 Fragments arranged by size: 516 385 247 83 60 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 781 1419 Size: 781 638 MscI TGG'CCA Cuts at: 0 364 1419 Size: 364 1055 MslI CAynn'nnrTG Cuts at: 0 1049 1419 Size: 1049 370 NspI r_CATG'y Cuts at: 0 1315 1419 Size: 1315 104 PflMI CCAn_nnn'nTGG Cuts at: 0 1006 1419 Size: 1006 413 PfoI T'CCnGG_A Cuts at: 0 1024 1419 Size: 1024 395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 121 153 958 990 1419 Size: 121 32 805 32 429 Fragments arranged by size: 805 429 121 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1075 1371 1419 Size: 1075 296 48 Fragments arranged by size: 1075 296 48 PstI C_TGCA'G Cuts at: 0 1137 1419 Size: 1137 282 SapI GCTCTTCn'nnn_ Cuts at: 0 347 522 1419 Size: 347 175 897 Fragments arranged by size: 897 347 175 SfcI C'TryA_G Cuts at: 0 494 1133 1419 Size: 494 639 286 Fragments arranged by size: 639 494 286 SmlI C'TyrA_G Cuts at: 0 808 1419 Size: 808 611 SphI G_CATG'C Cuts at: 0 1315 1419 Size: 1315 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 767 1419 Size: 767 652 TatI w'GTAC_w Cuts at: 0 537 1419 Size: 537 882 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 50 82 1419 Size: 50 32 1337 Fragments arranged by size: 1337 50 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1099 1419 Size: 1099 320 Enzymes that do cut: AcuI AfeI AhdI AloI ApaLI ApoI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstAPI BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII MscI MslI NspI PflMI PfoI PpiI PpuMI PstI SapI SfcI SmlI SphI TaqII TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI