Restriction map for pFN21AE1017
 (Linear) (Six-base) MAPSORT of: KIEE1017.seq  Check: 8898  from: 1  to: 1419



KIEE1017.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     971     974    1200    1285    1419 
   Size:        413     558       3     226      85     134

  Fragments arranged by size: 

                 558     413     226     134      85       3
 
AfeI AGC'GCT

Cuts at:      0    1255    1419 
   Size:       1255     164
 
AhdI GACnn_n'nnGTC

Cuts at:      0     678    1419 
   Size:        678     741
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     958     990    1419 
   Size:        958      32     429

  Fragments arranged by size: 

                 958     429      32
 
ApaLI G'TGCA_C

Cuts at:      0     800    1419 
   Size:        800     619
 
ApoI r'AATT_y

Cuts at:      0     407     440     623    1419 
   Size:        407      33     183     796

  Fragments arranged by size: 

                 796     407     183      33
 
BanII G_rGCy'C

Cuts at:      0     183     583    1308    1419 
   Size:        183     400     725     111

  Fragments arranged by size: 

                 725     400     183     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1297    1419 
   Size:       1297     122
 
BbvCI CC'TCA_GC

Cuts at:      0     704    1419 
   Size:        704     715
 
BglI GCCn_nnn'nGGC

Cuts at:      0     990    1419 
   Size:        990     429
 
Bme1580I G_kGCm'C

Cuts at:      0     804    1419 
   Size:        804     615
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     529     616    1419 
   Size:        529      87     803

  Fragments arranged by size: 

                 803     529      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     929     962    1322    1419 
   Size:        283     646      33     360      97

  Fragments arranged by size: 

                 646     360     283      97      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     704    1419 
   Size:        704     715
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829    1419 
   Size:        829     590
 
BsaHI Gr'CG_yC

Cuts at:      0     318    1419 
   Size:        318    1101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     756     786     958     988    1419 
   Size:        756      30     172      30     431

  Fragments arranged by size: 

                 756     431     172      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     658     661     664     692     895     926    1419 
   Size:        658       3       3      28     203      31     493

  Fragments arranged by size: 

                 658     493     203      31      28       3       3
 
BseYI C'CCAG_C

Cuts at:      0      51    1391    1419 
   Size:         51    1340      28

  Fragments arranged by size: 

                1340      51      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     812    1419 
   Size:        181     631     607

  Fragments arranged by size: 

                 631     607     181
 
BsiHKAI G_wGCw'C

Cuts at:      0     403     804    1286    1419 
   Size:        403     401     482     133

  Fragments arranged by size: 

                 482     403     401     133
 
BsmI GAATG_Cn'

Cuts at:      0     509    1419 
   Size:        509     910
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     256     325    1419 
   Size:        256      69    1094

  Fragments arranged by size: 

                1094     256      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     403     583     804    1286    1308    1419 
   Size:        183     220     180     221     482      22     111

  Fragments arranged by size: 

                 482     221     220     183     180     111      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      52     375    1419 
   Size:         52     323    1044

  Fragments arranged by size: 

                1044     323      52
 
BsrFI r'CCGG_y

Cuts at:      0    1196    1419 
   Size:       1196     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1206    1419 
   Size:       1206     213
 
BstYI r'GATC_y

Cuts at:      0    1038    1419 
   Size:       1038     381
 
Bsu36I CC'TnA_GG

Cuts at:      0     875    1419 
   Size:        875     544
 
BtgI C'CryG_G

Cuts at:      0      58     450    1419 
   Size:         58     392     969

  Fragments arranged by size: 

                 969     392      58
 
BtsI GCAGTG_nn'

Cuts at:      0     197     754     829    1184    1397    1419 
   Size:        197     557      75     355     213      22

  Fragments arranged by size: 

                 557     355     213     197      75      22
 
EaeI y'GGCC_r

Cuts at:      0     362    1329    1419 
   Size:        362     967      90

  Fragments arranged by size: 

                 967     362      90
 
EarI CTCTTCn'nnn_

Cuts at:      0     347     522     635    1419 
   Size:        347     175     113     784

  Fragments arranged by size: 

                 784     347     175     113
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     313    1351    1419 
   Size:        313    1038      68

  Fragments arranged by size: 

                1038     313      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     413     929     962     971     974    1200    1285 
   Size:        283     130     516      33       9       3     226      85

Cuts at:   1285    1322    1419 
   Size:         37      97

  Fragments arranged by size: 

                 516     283     226     130      97      85      37      33
                   9       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     160     587    1378    1419 
   Size:        160     427     791      41

  Fragments arranged by size: 

                 791     427     160      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1075    1371    1419 
   Size:       1075     296      48

  Fragments arranged by size: 

                1075     296      48
 
EcoRV GAT'ATC

Cuts at:      0     887    1419 
   Size:        887     532
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     584    1419 
   Size:        584     835
 
HaeII r_GCGC'y

Cuts at:      0    1257    1419 
   Size:       1257     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     385     417     664     696     756     788     871     903 
   Size:        385      32     247      32      60      32      83      32

Cuts at:    903    1419 
   Size:        516

  Fragments arranged by size: 

                 516     385     247      83      60      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     781    1419 
   Size:        781     638
 
MscI TGG'CCA

Cuts at:      0     364    1419 
   Size:        364    1055
 
MslI CAynn'nnrTG

Cuts at:      0    1049    1419 
   Size:       1049     370
 
NspI r_CATG'y

Cuts at:      0    1315    1419 
   Size:       1315     104
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1006    1419 
   Size:       1006     413
 
PfoI T'CCnGG_A

Cuts at:      0    1024    1419 
   Size:       1024     395
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     958     990    1419 
   Size:        121      32     805      32     429

  Fragments arranged by size: 

                 805     429     121      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1075    1371    1419 
   Size:       1075     296      48

  Fragments arranged by size: 

                1075     296      48
 
PstI C_TGCA'G

Cuts at:      0    1137    1419 
   Size:       1137     282
 
SapI GCTCTTCn'nnn_

Cuts at:      0     347     522    1419 
   Size:        347     175     897

  Fragments arranged by size: 

                 897     347     175
 
SfcI C'TryA_G

Cuts at:      0     494    1133    1419 
   Size:        494     639     286

  Fragments arranged by size: 

                 639     494     286
 
SmlI C'TyrA_G

Cuts at:      0     808    1419 
   Size:        808     611
 
SphI G_CATG'C

Cuts at:      0    1315    1419 
   Size:       1315     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     767    1419 
   Size:        767     652
 
TatI w'GTAC_w

Cuts at:      0     537    1419 
   Size:        537     882
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      50      82    1419 
   Size:         50      32    1337

  Fragments arranged by size: 

                1337      50      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1099    1419 
   Size:       1099     320
 

 Enzymes that do cut:

     AcuI     AfeI     AhdI     AloI    ApaLI     ApoI    BanII     BbsI
    BbvCI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrFI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII
    Hin4I  HindIII     MscI     MslI     NspI    PflMI     PfoI     PpiI
    PpuMI     PstI     SapI     SfcI     SmlI     SphI    TaqII     TatI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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