Restriction map for
pFN21AE1059
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AlwI ApoI AscI
AseI AsnI Asp700I Asp718I BamHI
BbrPI BcgI BciVI BclI BfrI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BstBI
ClaI DraI DraIII DrdI EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI ScaI SfiI
SfuI SnaBI SpeI SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 1079 ACGTGT A^C[GA][CT]GT
AhdI 2389 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1651 GTGCAC G^TGCAC
Aor51HI 27 AGCGCT AGC^GCT
ApaI 2209 GGGCCC GGGCC^C
ApaLI 1651 GTGCAC G^TGCAC
AspEI 2389 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2423 TGCGCA TGC^GCA
AvrII 2589 CCTAGG C^CTAGG
BbeI 149 GGCGCC GGCGC^C
BbsI 2028 GAAGAC GAAGAC(2/6)
BfaI 2590 CTAG C^TAG
BlnI 2589 CCTAGG C^CTAGG
BpuAI 2028 GAAGAC GAAGAC(2/6)
BsiEI 1070 CGGCCG CG[GA][CT]^CG
BspHI 86 TCATGA T^CATGA
BssSI 1546 CACGAG CACGAG(-5/-1)
Bst1107I 667 GTATAC GTA^TAC
BstEII 2433 GGTGACC G^GT[ACGT]ACC
BstPI 2433 GGTGACC G^GT[ACGT]ACC
BstZ17I 667 GTATAC GTA^TAC
CpoI 2216 CGGACCG CG^G[AT]CCG
EagI 1070 CGGCCG C^GGCCG
Eam1105I 2389 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1070 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 1070 CGGCCG C^GGCCG
EcoNI 489 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2433 GGTGACC G^GT[ACGT]ACC
FspI 2423 TGCGCA TGC^GCA
HincII 1213 GTCGAC GT[CT]^[GA]AC
HindII 1213 GTCGAC GT[CT]^[GA]AC
HindIII 1618 AAGCTT A^AGCTT
KasI 149 GGCGCC G^GCGCC
KspI 2522 CCGCGG CCGC^GG
MaeI 2590 CTAG C^TAG
NarI 149 GGCGCC GG^CGCC
RcaI 86 TCATGA T^CATGA
RsrII 2216 CGGACCG CG^G[AT]CCG
SacI 61 GAGCTC GAGCT^C
SacII 2522 CCGCGG CCGC^GG
SalI 1213 GTCGAC G^TCGAC
SbfI 1509 CCTGCAGG CCTGCA^GG
SfoI 149 GGCGCC GGC^GCC
SgrAI 1449 CGCCGGTG C[GA]^CCGG[CT]G
SphI 1611 GCATGC GCATG^C
Sse8387I 1509 CCTGCAGG CCTGCA^GG
StuI 375 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 34 enzymes) #
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AocI 1189 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
AspHI 61 GAGCTC G[AT]GC[AT]^C
1651 GTGCAC
AspI 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1993 GACTACGTC
BanII 61 GAGCTC G[GA]GC[CT]^C
2209 GGGCCC
BbvCI 253 CCTCAGC CCTCAGC(-5/-2)
2358 CCTCAGC
BglII 182 AGATCT A^GATCT
2327 AGATCT
BlpI 367 GCTCAGC GC^T[ACGT]AGC
1620 GCTTAGC
Bpu1102I 367 GCTCAGC GC^T[ACGT]AGC
1620 GCTTAGC
BsaI 839 GAGACC GGTCTC(1/5)
973 GAGACC
BseRI 970 GAGGAG GAGGAG(10/8)
2017 GAGGAG
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
1651 GTGCAC
BsrDI 1580 GCAATG GCAATG(2/0)
1796 GCAATG
BstAPI 1503 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2223 GCAGCTGCTGC
BstYI 182 AGATCT [GA]^GATC[CT]
2327 AGATCT
Bsu36I 1189 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
CelII 367 GCTCAGC GC^T[ACGT]AGC
1620 GCTTAGC
DpnII 183 GATC ^GATC
2328 GATC
EarI 1810 CTCTTC CTCTTC(1/4)
2233 CTCTTC
Eco81I 1189 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
Ksp632I 1810 CTCTTC CTCTTC(1/4)
2233 CTCTTC
MboI 183 GATC ^GATC
2328 GATC
MflI 182 AGATCT [GA]^GATC[CT]
2327 AGATCT
NaeI 1068 GCCGGC GCC^GGC
1625 GCCGGC
NcoI 590 CCATGG C^CATGG
812 CCATGG
NdeII 183 GATC ^GATC
2328 GATC
NgoMIV 1068 GCCGGC G^CCGGC
1625 GCCGGC
NspI 194 GCATGT [GA]CATG^[CT]
1611 GCATGC
PflFI 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1993 GACTACGTC
SapI 1809 GCTCTTC GCTCTTC(1/4)
2232 GCTCTTC
Sau3AI 183 GATC ^GATC
2328 GATC
SmlI 178 CTCAAG C^T[CT][GA]AG
1717 CTTGAG
Tth111I 334 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1993 GACTACGTC
XcmI 249 CCAGCCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2324 CCAAGATCTTTGTGG
XhoII 182 AGATCT [GA]^GATC[CT]
2327 AGATCT
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI AvaI AvaII BalI
BanI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XmaCI XmaI