(Circular) (Six-base) MAPSORT of: pFN21AE1059.seq Check: 5731 from: 1 to: 7315 pFN21AE1059 7315 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2730 2730 Size: 7315 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5125 278 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 2673 2919 3219 4658 1937 Size: 409 327 246 300 1439 4594 Fragments arranged by size: 4594 1439 409 327 300 246 Acc65I G'GTAC_C Cuts at: 4636 4636 Size: 7315 AclI AA'CG_TT Cuts at: 1563 5442 5815 1563 Size: 3879 373 3063 Fragments arranged by size: 3879 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3802 4127 5378 6762 7030 1632 Size: 2170 325 1251 1384 268 1917 Fragments arranged by size: 2170 1917 1384 1251 325 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 7315 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7118 AflIII A'CryG_T Cuts at: 1203 3084 6214 7191 1203 Size: 1881 3130 977 1327 Fragments arranged by size: 3130 1881 1327 977 AgeI A'CCGG_T Cuts at: 6146 6146 Size: 7315 AhdI GACnn_n'nnGTC Cuts at: 4399 6044 4399 Size: 1645 5670 AleI CACnn'nnGTG Cuts at: 1166 2654 1166 Size: 1488 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2966 3819 1184 Size: 1782 853 4680 Fragments arranged by size: 4680 1782 853 AlwNI CAG_nnn'CTG Cuts at: 2122 2225 2516 3514 3667 4105 4234 5058 6630 Size: 103 291 998 153 438 129 824 1572 Cuts at: 6630 2122 Size: 2807 Fragments arranged by size: 2807 1572 998 824 438 291 153 129 103 ApaI G_GGCC'C Cuts at: 4218 4218 Size: 7315 ApaLI G'TGCA_C Cuts at: 3656 5372 6528 3656 Size: 1716 1156 4443 Fragments arranged by size: 4443 1716 1156 ApoI r'AATT_y Cuts at: 1493 4624 4850 5002 7111 1493 Size: 3131 226 152 2109 1697 Fragments arranged by size: 3131 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5869 160 Size: 872 4837 1606 Fragments arranged by size: 4837 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 AvaI C'yCGr_G Cuts at: 1870 1943 2395 2731 3151 3764 3841 4192 4640 Size: 73 452 336 420 613 77 351 448 Cuts at: 4640 1870 Size: 4545 Fragments arranged by size: 4545 613 452 448 420 351 336 77 73 AvrII C'CTAG_G Cuts at: 4594 4594 Size: 7315 BamHI G'GATC_C Cuts at: 4645 5012 4645 Size: 367 6948 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2154 2353 2503 2545 2644 Size: 325 217 627 367 199 150 42 99 Cuts at: 2644 3155 3238 3860 4636 6091 618 Size: 511 83 622 776 1455 1842 Fragments arranged by size: 1842 1455 776 627 622 511 367 325 217 199 150 99 83 42 BanII G_rGCy'C Cuts at: 1391 2070 4218 4634 1391 Size: 679 2148 416 4072 Fragments arranged by size: 4072 2148 679 416 BbeI G_GCGC'C Cuts at: 2158 2158 Size: 7315 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4040 928 Size: 971 2141 4203 Fragments arranged by size: 4203 2141 971 BbvCI CC'TCA_GC Cuts at: 2259 4364 2259 Size: 2105 5210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5506 5540 1903 Size: 34 3569 34 3678 Fragments arranged by size: 3678 3569 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5207 6417 5207 Size: 1210 6105 BclI T'GATC_A Cuts at: 1548 1548 Size: 7315 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2127 2221 2249 Size: 107 122 71 631 605 455 94 28 Cuts at: 2249 2512 2790 3705 3955 5926 136 Size: 263 278 915 250 1971 1525 Fragments arranged by size: 1971 1525 915 631 605 455 278 263 250 122 107 94 71 28 BglII A'GATC_T Cuts at: 2187 4332 7310 2187 Size: 2145 2978 2192 Fragments arranged by size: 2978 2192 2145 BlpI GC'TnA_GC Cuts at: 2373 3626 4732 2373 Size: 1253 1106 4956 Fragments arranged by size: 4956 1253 1106 Bme1580I G_kGCm'C Cuts at: 1790 2358 2508 2621 2649 2760 2874 2955 2994 Size: 568 150 113 28 111 114 81 39 Cuts at: 2994 3129 3158 3174 3613 3660 3765 4218 5376 Size: 135 29 16 439 47 105 453 1158 Cuts at: 5376 6532 1790 Size: 1156 2573 Fragments arranged by size: 2573 1158 1156 568 453 439 150 135 114 113 111 105 81 47 39 29 28 16 BmgBI CAC'GTC Cuts at: 1318 2869 3085 3094 3124 3655 4426 1318 Size: 1551 216 9 30 531 771 4207 Fragments arranged by size: 4207 1551 771 531 216 30 9 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2290 2871 3474 Size: 416 53 83 399 24 873 581 603 Cuts at: 3474 5046 6004 442 Size: 1572 958 1753 Fragments arranged by size: 1753 1572 958 873 603 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2538 2876 3045 3277 3386 3732 4112 Size: 884 415 338 169 232 109 346 380 Cuts at: 4112 4268 5975 1239 Size: 156 1707 2579 Fragments arranged by size: 2579 1707 884 415 380 346 338 232 169 156 109 Bpu10I CC'TnA_GC Cuts at: 2259 4364 2259 Size: 2105 5210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3743 5440 6305 6603 2168 Size: 1575 1697 865 298 2880 Fragments arranged by size: 2880 1697 1575 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2838 2972 5978 882 Size: 617 1339 134 3006 2219 Fragments arranged by size: 3006 2219 1339 617 134 BsaAI yAC'GTr Cuts at: 493 1204 5106 493 Size: 711 3902 2702 Fragments arranged by size: 3902 2702 711 BsaBI GATnn'nnATC Cuts at: 7309 7309 Size: 7315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2155 3771 4172 4293 Size: 53 83 186 936 622 1616 401 121 Cuts at: 4293 5122 5504 6139 275 Size: 829 382 635 1451 Fragments arranged by size: 1616 1451 936 829 635 622 401 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 5748 6146 6420 6567 6978 5748 Size: 398 274 147 411 6085 Fragments arranged by size: 6085 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3460 3490 1178 Size: 30 165 30 2057 30 5003 Fragments arranged by size: 5003 2057 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2990 4037 1186 Size: 1804 1047 4464 Fragments arranged by size: 4464 1804 1047 BseYI C'CCAG_C Cuts at: 2253 2278 2331 2363 2402 2425 2477 2610 2685 Size: 25 53 32 39 23 52 133 75 Cuts at: 2685 2780 3247 3265 4561 4575 6518 7014 2253 Size: 95 467 18 1296 14 1943 496 2554 Fragments arranged by size: 2554 1943 1296 496 467 133 95 75 53 52 39 32 25 23 18 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3527 3779 3851 1163 Size: 573 1791 252 72 4627 Fragments arranged by size: 4627 1791 573 252 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3078 4765 5526 6554 1540 Size: 275 186 1077 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1077 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 3660 5376 5461 6532 2070 Size: 1590 1716 85 1071 2853 Fragments arranged by size: 2853 1716 1590 1071 85 BsmI GAATG_Cn' Cuts at: 4826 4919 4826 Size: 93 7222 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2358 2508 2621 2649 2760 2874 Size: 399 280 288 150 113 28 111 114 Cuts at: 2874 2955 2994 3129 3158 3174 3613 3660 3765 Size: 81 39 135 29 16 439 47 105 Cuts at: 3765 4218 4634 5376 5461 6532 1391 Size: 453 416 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 439 416 399 288 280 150 135 114 113 111 105 85 81 47 39 29 28 16 BspEI T'CCGG_A Cuts at: 6978 6978 Size: 7315 BspHI T'CATG_A Cuts at: 2091 5204 6934 2091 Size: 3113 1730 2472 Fragments arranged by size: 3113 2472 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2730 3522 4464 4670 844 Size: 1886 792 942 206 3489 Fragments arranged by size: 3489 1886 942 792 206 BsrBI CCG'CTC Cuts at: 1394 1823 5202 1394 Size: 429 3379 3507 Fragments arranged by size: 3507 3379 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3592 3808 5810 5984 65 Size: 1183 2344 216 2002 174 1396 Fragments arranged by size: 2344 2002 1396 1183 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3073 3299 3455 3630 3735 4224 5959 Size: 110 1263 226 156 175 105 489 1735 Cuts at: 5959 6146 1700 Size: 187 2869 Fragments arranged by size: 2869 1735 1263 489 226 187 175 156 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7315 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7315 BssSI C'ACGA_G Cuts at: 3551 5375 6387 3551 Size: 1824 1012 4479 Fragments arranged by size: 4479 1824 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3514 4234 794 Size: 2720 720 3875 Fragments arranged by size: 3875 2720 720 BstBI TT'CG_AA Cuts at: 6119 6119 Size: 7315 BstEII G'GTnAC_C Cuts at: 1184 4438 1184 Size: 3254 4061 BstXI CCAn_nnnn'nTGG Cuts at: 2656 3781 4288 2656 Size: 1125 507 5683 Fragments arranged by size: 5683 1125 507 BstYI r'GATC_y Cuts at: 1972 2187 4332 4645 5012 5397 5414 6866 6952 Size: 215 2145 313 367 385 17 1452 86 Cuts at: 6952 6964 7310 1972 Size: 12 346 1977 Fragments arranged by size: 2145 1977 1452 385 367 346 313 215 86 17 12 BstZ17I GTA'TAC Cuts at: 2674 2674 Size: 7315 Bsu36I CC'TnA_GG Cuts at: 3195 3849 3195 Size: 654 6661 BtgI C'CryG_G Cuts at: 513 1065 2595 2817 4287 4308 4527 513 Size: 552 1530 222 1470 21 219 3301 Fragments arranged by size: 3301 1530 1470 552 222 219 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4503 487 Size: 683 56 3277 3299 Fragments arranged by size: 3299 3277 683 56 BtsI GCAGTG_nn' Cuts at: 2963 3809 3834 4259 4831 5625 5645 2963 Size: 846 25 425 572 794 20 4633 Fragments arranged by size: 4633 846 794 572 425 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2622 2657 529 Size: 35 2058 35 5187 Fragments arranged by size: 5187 2058 35 35 DraI TTT'AAA Cuts at: 4619 4619 Size: 7315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6322 809 Size: 507 5006 1802 Fragments arranged by size: 5006 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2049 2484 Size: 54 1425 50 275 18 195 24 435 Cuts at: 2484 2791 2839 3075 3528 3697 4161 4311 4485 Size: 307 48 236 453 169 464 150 174 Cuts at: 4485 4762 5651 7280 8 Size: 277 889 1629 43 Fragments arranged by size: 1629 1425 889 464 453 435 307 277 275 236 195 174 169 150 54 50 48 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3075 4762 1537 Size: 275 1263 1687 4090 Fragments arranged by size: 4090 1687 1263 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3821 4244 5245 1353 Size: 2468 423 1001 3423 Fragments arranged by size: 3423 2468 1001 423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4574 5902 6276 6422 387 Size: 1064 138 2985 1328 374 146 1280 Fragments arranged by size: 2985 1328 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2068 Size: 7315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2538 2876 3045 3277 3386 3732 Size: 393 491 415 338 169 232 109 346 Cuts at: 3732 3802 4112 4127 4268 5378 5975 6762 7030 Size: 70 310 15 141 1110 597 787 268 Cuts at: 7030 1239 Size: 1524 Fragments arranged by size: 1524 1110 787 597 491 415 393 346 338 310 268 232 169 141 109 70 15 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2498 Size: 7315 EcoO109I rG'GnC_Cy Cuts at: 2119 2222 2399 2513 2522 2584 2831 2890 3388 Size: 103 177 114 9 62 247 59 498 Cuts at: 3388 3511 3685 3727 3845 4214 4215 2119 Size: 123 174 42 118 369 1 5219 Fragments arranged by size: 5219 498 369 247 177 174 123 118 114 103 62 59 42 9 1 EcoRI G'AATT_C Cuts at: 4624 5002 4624 Size: 378 6937 FspI TGC'GCA Cuts at: 4430 5821 4430 Size: 1391 5924 HaeII r_GCGC'y Cuts at: 1678 2036 2158 2285 3283 3420 3456 4371 6462 Size: 358 122 127 998 137 36 915 2091 Cuts at: 6462 1678 Size: 2531 Fragments arranged by size: 2531 2091 998 915 358 137 127 122 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2449 2481 4385 Size: 32 135 32 482 32 363 32 1904 Cuts at: 4385 4417 5956 5988 6030 6062 1373 Size: 32 1539 32 42 32 2626 Fragments arranged by size: 2626 1904 1539 482 363 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3220 4659 4905 669 Size: 988 281 1282 1439 246 3079 Fragments arranged by size: 3079 1439 1282 988 281 246 HindIII A'AGCT_T Cuts at: 748 3623 748 Size: 2875 4440 HpaI GTT'AAC Cuts at: 4905 4905 Size: 7315 KasI G'GCGC_C Cuts at: 2154 2154 Size: 7315 KpnI G_GTAC'C Cuts at: 4640 4640 Size: 7315 MfeI C'AATT_G Cuts at: 4914 4914 Size: 7315 MluI A'CGCG_T Cuts at: 7191 7191 Size: 7315 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 3540 6429 6613 1305 Size: 1623 612 2889 184 2007 Fragments arranged by size: 2889 2007 1623 612 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2793 4163 4313 4487 Size: 54 1425 343 195 766 1370 150 174 Cuts at: 4487 10 Size: 2838 Fragments arranged by size: 2838 1425 1370 766 343 195 174 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2306 2654 3801 5273 5632 5791 Size: 648 930 210 348 1147 1472 359 159 Cuts at: 5791 518 Size: 2042 Fragments arranged by size: 2042 1472 1147 930 648 359 348 210 159 MspA1I CmG'CkG Cuts at: 1606 2610 3251 3502 3637 3781 4090 4105 4231 Size: 1004 641 251 135 144 309 15 126 Cuts at: 4231 4502 4529 4603 4731 5408 6556 6801 1606 Size: 271 27 74 128 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 641 309 271 251 245 144 135 128 126 74 27 15 NaeI GCC'GGC Cuts at: 3075 3632 3075 Size: 557 6758 NarI GG'CG_CC Cuts at: 2155 2155 Size: 7315 NcoI C'CATG_G Cuts at: 513 1065 2595 2817 513 Size: 552 1530 222 5011 Fragments arranged by size: 5011 1530 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 7315 NgoMIV G'CCGG_C Cuts at: 3073 3630 3073 Size: 557 6758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7315 NotI GC'GGCC_GC Cuts at: 4762 4762 Size: 7315 NspI r_CATG'y Cuts at: 1132 1229 2203 3620 4673 6218 1132 Size: 97 974 1417 1053 1545 2229 Fragments arranged by size: 2229 1545 1417 1053 974 97 PasI CC'CwG_GG Cuts at: 1348 2510 1348 Size: 1162 6153 PciI A'CATG_T Cuts at: 6214 6214 Size: 7315 PflMI CCAn_nnn'nTGG Cuts at: 1108 2516 3436 3667 4159 4186 4378 4540 1108 Size: 1408 920 231 492 27 192 162 3883 Fragments arranged by size: 3883 1408 920 492 231 192 162 27 PmeI GTTT'AAAC Cuts at: 4619 4619 Size: 7315 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5384 5416 6897 6929 5384 Size: 32 1481 32 5770 Fragments arranged by size: 5770 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2399 2584 2831 3511 3727 2399 Size: 185 247 680 216 5987 Fragments arranged by size: 5987 680 247 216 185 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7315 PsiI TTA'TAA Cuts at: 4885 4885 Size: 7315 PspOMI G'GGCC_C Cuts at: 4214 4214 Size: 7315 PstI C_TGCA'G Cuts at: 830 3405 3519 3642 4667 830 Size: 2575 114 123 1025 3478 Fragments arranged by size: 3478 2575 1025 123 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7315 PvuII CAG'CTG Cuts at: 2610 3251 3637 3781 4090 4105 4231 4603 2610 Size: 641 386 144 309 15 126 372 5322 Fragments arranged by size: 5322 641 386 372 309 144 126 15 RsrII CG'GwC_CG Cuts at: 4222 4222 Size: 7315 SacI G_AGCT'C Cuts at: 2070 2070 Size: 7315 SacII CC_GC'GG Cuts at: 4530 4530 Size: 7315 SalI G'TCGA_C Cuts at: 1936 3218 4657 1936 Size: 1282 1439 4594 Fragments arranged by size: 4594 1439 1282 SanDI GG'GwC_CC Cuts at: 2399 2831 2399 Size: 432 6883 SapI GCTCTTCn'nnn_ Cuts at: 3821 4244 3821 Size: 423 6892 SbfI CC_TGCA'GG Cuts at: 3519 4667 3519 Size: 1148 6167 ScaI AGT'ACT Cuts at: 5563 7275 5563 Size: 1712 5603 SexAI A'CCwGG_T Cuts at: 2349 2499 2541 2580 2349 Size: 150 42 39 7084 Fragments arranged by size: 7084 150 42 39 SfcI C'TryA_G Cuts at: 826 1045 3401 3515 3638 4010 4447 4663 5798 Size: 219 2356 114 123 372 437 216 1135 Cuts at: 5798 6479 6670 826 Size: 681 191 1471 Fragments arranged by size: 2356 1471 1135 681 437 372 219 216 191 123 114 SfoI GGC'GCC Cuts at: 2156 2156 Size: 7315 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7315 SgrAI Cr'CCGG_yG Cuts at: 1700 3455 1700 Size: 1755 5560 SmaI CCC'GGG Cuts at: 1872 2397 2733 3153 3843 4194 4642 1872 Size: 525 336 420 690 351 448 4545 Fragments arranged by size: 4545 690 525 448 420 351 336 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 3722 5419 6320 6582 820 Size: 197 926 240 1539 1697 901 262 1553 Fragments arranged by size: 1697 1553 1539 926 901 262 240 197 SnaBI TAC'GTA Cuts at: 493 5106 493 Size: 4613 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7315 SphI G_CATG'C Cuts at: 1132 3620 4673 1132 Size: 2488 1053 3774 Fragments arranged by size: 3774 2488 1053 SrfI GCCC'GGGC Cuts at: 3153 3153 Size: 7315 SspI AAT'ATT Cuts at: 5 52 5239 5 Size: 47 5187 2081 Fragments arranged by size: 5187 2081 47 StuI AGG'CCT Cuts at: 1517 2382 1517 Size: 865 6450 StyI C'CwwG_G Cuts at: 513 1065 2595 2817 3006 3333 3828 4413 4494 Size: 552 1530 222 189 327 495 585 81 Cuts at: 4494 4594 4754 513 Size: 100 160 3074 Fragments arranged by size: 3074 1530 585 552 495 327 222 189 160 100 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4589 5342 5359 5512 5697 6142 325 Size: 1255 3009 753 17 153 185 445 1498 Fragments arranged by size: 3009 1498 1255 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 5561 7273 Size: 274 80 33 51 1443 321 3263 1712 Cuts at: 7273 96 Size: 138 Fragments arranged by size: 3263 1712 1443 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 7275 7307 2117 Size: 32 5126 32 2125 Fragments arranged by size: 5126 2125 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2342 4001 1316 Size: 1026 1659 4630 Fragments arranged by size: 4630 1659 1026 XbaI T'CTAG_A Cuts at: 4651 4651 Size: 7315 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2261 4336 2261 Size: 2075 5240 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7315 XmaI C'CCGG_G Cuts at: 1870 2395 2731 3151 3841 4192 4640 1870 Size: 525 336 420 690 351 448 4545 Fragments arranged by size: 4545 690 525 448 420 351 336 XmnI GAAnn'nnTTC Cuts at: 5444 5444 Size: 7315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5123 276 Size: 53 83 186 936 3589 2468 Fragments arranged by size: 3589 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SfiI SwaI