Restriction map for
pFN21AE1059
(Linear) (Six-base) MAPSORT of: KIEE1059.seq Check: 8396 from: 1 to: 2610
KIEE1059.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 725 2610
Size: 725 1885
AccI GT'mk_AC
Cuts at: 0 341 668 914 1214 2610
Size: 341 327 246 300 1396
Fragments arranged by size:
1396 341 327 300 246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1797 2122 2610
Size: 1797 325 488
Fragments arranged by size:
1797 488 325
AfeI AGC'GCT
Cuts at: 0 29 2610
Size: 29 2581
AflIII A'CryG_T
Cuts at: 0 1079 2610
Size: 1079 1531
AhdI GACnn_n'nnGTC
Cuts at: 0 2394 2610
Size: 2394 216
AleI CACnn'nnGTG
Cuts at: 0 649 2610
Size: 649 1961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 1814 2610
Size: 961 853 796
Fragments arranged by size:
961 853 796
AlwNI CAG_nnn'CTG
Cuts at: 0 117 220 511 1509 1662 2100 2229 2610
Size: 117 103 291 998 153 438 129 381
Fragments arranged by size:
998 438 381 291 153 129 117 103
ApaI G_GGCC'C
Cuts at: 0 2213 2610
Size: 2213 397
ApaLI G'TGCA_C
Cuts at: 0 1651 2610
Size: 1651 959
AvaI C'yCGr_G
Cuts at: 0 390 726 1146 1759 1836 2187 2610
Size: 390 336 420 613 77 351 423
Fragments arranged by size:
613 423 420 390 351 336 77
AvrII C'CTAG_G
Cuts at: 0 2589 2610
Size: 2589 21
BanI G'GyrC_C
Cuts at: 0 149 348 498 540 639 1150 1233 1855
Size: 149 199 150 42 99 511 83 622
Cuts at: 1855 2610
Size: 755
Fragments arranged by size:
755 622 511 199 150 149 99 83
42
BanII G_rGCy'C
Cuts at: 0 65 2213 2610
Size: 65 2148 397
Fragments arranged by size:
2148 397 65
BbeI G_GCGC'C
Cuts at: 0 153 2610
Size: 153 2457
BbsI GAAGACnn'nnnn_
Cuts at: 0 2035 2610
Size: 2035 575
BbvCI CC'TCA_GC
Cuts at: 0 254 2359 2610
Size: 254 2105 251
Fragments arranged by size:
2105 254 251
BglI GCCn_nnn'nGGC
Cuts at: 0 122 216 244 507 785 1700 1950 2610
Size: 122 94 28 263 278 915 250 660
Fragments arranged by size:
915 660 278 263 250 122 94 28
BglII A'GATC_T
Cuts at: 0 182 2327 2610
Size: 182 2145 283
Fragments arranged by size:
2145 283 182
BlpI GC'TnA_GC
Cuts at: 0 368 1621 2610
Size: 368 1253 989
Fragments arranged by size:
1253 989 368
Bme1580I G_kGCm'C
Cuts at: 0 353 503 616 644 755 869 950 989
Size: 353 150 113 28 111 114 81 39
Cuts at: 989 1124 1153 1169 1608 1655 1760 2213 2610
Size: 135 29 16 439 47 105 453 397
Fragments arranged by size:
453 439 397 353 150 135 114 113
111 105 81 47 39 29 28 16
BmgBI CAC'GTC
Cuts at: 0 864 1080 1089 1119 1650 2421 2610
Size: 864 216 9 30 531 771 189
Fragments arranged by size:
864 771 531 216 189 30 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 866 1469 2610
Size: 285 581 603 1141
Fragments arranged by size:
1141 603 581 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 533 871 1040 1272 1381 1727 2107
Size: 118 415 338 169 232 109 346 380
Cuts at: 2107 2263 2610
Size: 156 347
Fragments arranged by size:
415 380 347 346 338 232 169 156
118 109
Bpu10I CC'TnA_GC
Cuts at: 0 254 2359 2610
Size: 254 2105 251
Fragments arranged by size:
2105 254 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1738 2610
Size: 163 1575 872
Fragments arranged by size:
1575 872 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 833 967 2610
Size: 833 134 1643
Fragments arranged by size:
1643 833 134
BsaHI Gr'CG_yC
Cuts at: 0 150 1766 2167 2288 2610
Size: 150 1616 401 121 322
Fragments arranged by size:
1616 401 322 150 121
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1455 1485 2610
Size: 1455 30 1125
Fragments arranged by size:
1455 1125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 985 2032 2610
Size: 985 1047 578
Fragments arranged by size:
1047 985 578
BseYI C'CCAG_C
Cuts at: 0 248 273 326 358 397 420 472 605
Size: 248 25 53 32 39 23 52 133
Cuts at: 605 680 775 1242 1260 2556 2570 2610
Size: 75 95 467 18 1296 14 40
Fragments arranged by size:
1296 467 248 133 95 75 53 52
40 39 32 25 23 18 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 1774 1846 2610
Size: 1522 252 72 764
Fragments arranged by size:
1522 764 252 72
BsiEI CG_ry'CG
Cuts at: 0 1073 2610
Size: 1073 1537
BsiHKAI G_wGCw'C
Cuts at: 0 65 1655 2610
Size: 65 1590 955
Fragments arranged by size:
1590 955 65
Bsp1286I G_dGCh'C
Cuts at: 0 65 353 503 616 644 755 869 950
Size: 65 288 150 113 28 111 114 81
Cuts at: 950 989 1124 1153 1169 1608 1655 1760 2213
Size: 39 135 29 16 439 47 105 453
Cuts at: 2213 2610
Size: 397
Fragments arranged by size:
453 439 397 288 150 135 114 113
111 105 81 65 47 39 29 28
16
BspHI T'CATG_A
Cuts at: 0 86 2610
Size: 86 2524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 725 1517 2459 2610
Size: 725 792 942 151
Fragments arranged by size:
942 792 725 151
BsrDI GCAATG_nn'
Cuts at: 0 1587 1803 2610
Size: 1587 216 807
Fragments arranged by size:
1587 807 216
BsrFI r'CCGG_y
Cuts at: 0 1068 1294 1450 1625 1730 2219 2610
Size: 1068 226 156 175 105 489 391
Fragments arranged by size:
1068 489 391 226 175 156 105
BssSI C'ACGA_G
Cuts at: 0 1546 2610
Size: 1546 1064
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1509 2229 2610
Size: 1509 720 381
Fragments arranged by size:
1509 720 381
BstEII G'GTnAC_C
Cuts at: 0 2433 2610
Size: 2433 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1776 2283 2610
Size: 651 1125 507 327
Fragments arranged by size:
1125 651 507 327
BstYI r'GATC_y
Cuts at: 0 182 2327 2610
Size: 182 2145 283
Fragments arranged by size:
2145 283 182
BstZ17I GTA'TAC
Cuts at: 0 669 2610
Size: 669 1941
Bsu36I CC'TnA_GG
Cuts at: 0 1190 1844 2610
Size: 1190 654 766
Fragments arranged by size:
1190 766 654
BtgI C'CryG_G
Cuts at: 0 590 812 2282 2303 2522 2610
Size: 590 222 1470 21 219 88
Fragments arranged by size:
1470 590 222 219 88 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2498 2610
Size: 2498 112
BtsI GCAGTG_nn'
Cuts at: 0 958 1804 1829 2254 2610
Size: 958 846 25 425 356
Fragments arranged by size:
958 846 425 356 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 617 652 2610
Size: 617 35 1958
Fragments arranged by size:
1958 617 35
EaeI y'GGCC_r
Cuts at: 0 20 44 479 786 834 1070 1523 1692
Size: 20 24 435 307 48 236 453 169
Cuts at: 1692 2156 2306 2480 2610
Size: 464 150 174 130
Fragments arranged by size:
464 453 435 307 236 174 169 150
130 48 24 20
EagI C'GGCC_G
Cuts at: 0 1070 2610
Size: 1070 1540
EarI CTCTTCn'nnn_
Cuts at: 0 1816 2239 2610
Size: 1816 423 371
Fragments arranged by size:
1816 423 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2569 2610
Size: 2569 41
EcoICRI GAG'CTC
Cuts at: 0 63 2610
Size: 63 2547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 533 871 1040 1272 1381 1727 1797
Size: 118 415 338 169 232 109 346 70
Cuts at: 1797 2107 2122 2263 2610
Size: 310 15 141 347
Fragments arranged by size:
415 347 346 338 310 232 169 141
118 109 70 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 2610
Size: 493 2117
EcoO109I rG'GnC_Cy
Cuts at: 0 114 217 394 508 517 579 826 885
Size: 114 103 177 114 9 62 247 59
Cuts at: 885 1383 1506 1680 1722 1840 2209 2210 2610
Size: 498 123 174 42 118 369 1 400
Fragments arranged by size:
498 400 369 247 177 174 123 118
114 114 103 62 59 42 9 1
FspI TGC'GCA
Cuts at: 0 2425 2610
Size: 2425 185
HaeII r_GCGC'y
Cuts at: 0 31 153 280 1278 1415 1451 2366 2610
Size: 31 122 127 998 137 36 915 244
Fragments arranged by size:
998 915 244 137 127 122 36 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 444 476 2380 2412 2610
Size: 49 32 363 32 1904 32 198
Fragments arranged by size:
1904 363 198 49 32 32 32
HincII GTy'rAC
Cuts at: 0 1215 2610
Size: 1215 1395
HindIII A'AGCT_T
Cuts at: 0 1618 2610
Size: 1618 992
KasI G'GCGC_C
Cuts at: 0 149 2610
Size: 149 2461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1535 2610
Size: 923 612 1075
Fragments arranged by size:
1075 923 612
MscI TGG'CCA
Cuts at: 0 22 788 2158 2308 2482 2610
Size: 22 766 1370 150 174 128
Fragments arranged by size:
1370 766 174 150 128 22
MslI CAynn'nnrTG
Cuts at: 0 91 301 649 1796 2610
Size: 91 210 348 1147 814
Fragments arranged by size:
1147 814 348 210 91
MspA1I CmG'CkG
Cuts at: 0 605 1246 1497 1632 1776 2085 2100 2226
Size: 605 641 251 135 144 309 15 126
Cuts at: 2226 2497 2524 2598 2610
Size: 271 27 74 12
Fragments arranged by size:
641 605 309 271 251 144 135 126
74 27 15 12
NaeI GCC'GGC
Cuts at: 0 1070 1627 2610
Size: 1070 557 983
Fragments arranged by size:
1070 983 557
NarI GG'CG_CC
Cuts at: 0 150 2610
Size: 150 2460
NcoI C'CATG_G
Cuts at: 0 590 812 2610
Size: 590 222 1798
Fragments arranged by size:
1798 590 222
NgoMIV G'CCGG_C
Cuts at: 0 1068 1625 2610
Size: 1068 557 985
Fragments arranged by size:
1068 985 557
NspI r_CATG'y
Cuts at: 0 198 1615 2610
Size: 198 1417 995
Fragments arranged by size:
1417 995 198
PasI CC'CwG_GG
Cuts at: 0 505 2610
Size: 505 2105
PflMI CCAn_nnn'nTGG
Cuts at: 0 511 1431 1662 2154 2181 2373 2535 2610
Size: 511 920 231 492 27 192 162 75
Fragments arranged by size:
920 511 492 231 192 162 75 27
PpuMI rG'GwC_Cy
Cuts at: 0 394 579 826 1506 1722 2610
Size: 394 185 247 680 216 888
Fragments arranged by size:
888 680 394 247 216 185
PspOMI G'GGCC_C
Cuts at: 0 2209 2610
Size: 2209 401
PstI C_TGCA'G
Cuts at: 0 1400 1514 1637 2610
Size: 1400 114 123 973
Fragments arranged by size:
1400 973 123 114
PvuII CAG'CTG
Cuts at: 0 605 1246 1632 1776 2085 2100 2226 2598
Size: 605 641 386 144 309 15 126 372
Cuts at: 2598 2610
Size: 12
Fragments arranged by size:
641 605 386 372 309 144 126 15
12
RsrII CG'GwC_CG
Cuts at: 0 2217 2610
Size: 2217 393
SacI G_AGCT'C
Cuts at: 0 65 2610
Size: 65 2545
SacII CC_GC'GG
Cuts at: 0 2525 2610
Size: 2525 85
SalI G'TCGA_C
Cuts at: 0 1213 2610
Size: 1213 1397
SanDI GG'GwC_CC
Cuts at: 0 394 826 2610
Size: 394 432 1784
Fragments arranged by size:
1784 432 394
SapI GCTCTTCn'nnn_
Cuts at: 0 1816 2239 2610
Size: 1816 423 371
Fragments arranged by size:
1816 423 371
SbfI CC_TGCA'GG
Cuts at: 0 1514 2610
Size: 1514 1096
SexAI A'CCwGG_T
Cuts at: 0 344 494 536 575 2610
Size: 344 150 42 39 2035
Fragments arranged by size:
2035 344 150 42 39
SfcI C'TryA_G
Cuts at: 0 1396 1510 1633 2005 2442 2610
Size: 1396 114 123 372 437 168
Fragments arranged by size:
1396 437 372 168 123 114
SfoI GGC'GCC
Cuts at: 0 151 2610
Size: 151 2459
SgrAI Cr'CCGG_yG
Cuts at: 0 1450 2610
Size: 1450 1160
SmaI CCC'GGG
Cuts at: 0 392 728 1148 1838 2189 2610
Size: 392 336 420 690 351 421
Fragments arranged by size:
690 421 420 392 351 336
SmlI C'TyrA_G
Cuts at: 0 178 1717 2610
Size: 178 1539 893
Fragments arranged by size:
1539 893 178
SphI G_CATG'C
Cuts at: 0 1615 2610
Size: 1615 995
SrfI GCCC'GGGC
Cuts at: 0 1148 2610
Size: 1148 1462
StuI AGG'CCT
Cuts at: 0 377 2610
Size: 377 2233
StyI C'CwwG_G
Cuts at: 0 590 812 1001 1328 1823 2408 2489 2589
Size: 590 222 189 327 495 585 81 100
Cuts at: 2589 2610
Size: 21
Fragments arranged by size:
590 585 495 327 222 189 100 81
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2584 2610
Size: 2584 26
TatI w'GTAC_w
Cuts at: 0 293 2610
Size: 293 2317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 2610
Size: 112 32 2466
Fragments arranged by size:
2466 112 32
Tth111I GACn'n_nGTC
Cuts at: 0 337 1996 2610
Size: 337 1659 614
Fragments arranged by size:
1659 614 337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 256 2331 2610
Size: 256 2075 279
Fragments arranged by size:
2075 279 256
XmaI C'CCGG_G
Cuts at: 0 390 726 1146 1836 2187 2610
Size: 390 336 420 690 351 423
Fragments arranged by size:
690 423 420 390 351 336
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AloI ApoI AscI
AseI AsiSI BaeI BamHI BcgI BciVI BclI BfrBI
BmtI BplI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BsrBI BsrGI BssHII BstBI ClaI DraI DraIII
DrdI EcoRI EcoRV FalI FseI FspAI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI ScaI SfiI SgfI SnaBI SpeI SspI
SwaI XbaI XhoI XmnI ZraI