Restriction map for
pFN21AB5451
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# NO CUT ENZYMES ( 128 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 465 GACGTC GACGT^C
AccI 139 GTCTAC GT^[AC][GT]AC
AflIII 1043 ACGTGT A^C[GA][CT]GT
AspHI 268 GAGCTC G[AT]GC[AT]^C
AvaII 356 GGACC G^G[AT]CC
BanI 130 GGCGCC G^G[CT][GA]CC
BbeI 130 GGCGCC GGCGC^C
BbsI 558 GTCTTC GAAGAC(2/6)
BcnI 221 CCCGG CC^[GC]GG
BglI 614 GCCATATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 558 GTCTTC GAAGAC(2/6)
BsaAI 1084 TACGTG [CT]AC^GT[GA]
BsaI 874 GAGACC GGTCTC(1/5)
BsaWI 116 ACCGGA [AT]^CCGG[AT]
BseRI 265 GAGGAG GAGGAG(10/8)
BsgI 1087 GTGCAG GTGCAG(16/14)
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
BspMI 55 GCAGGT ACCTGC(4/8)
BsrDI 194 GCAATG GCAATG(2/0)
BsrFI 190 GCCGGC [GA]^CCGG[CT]
BstAPI 950 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 984 GGTGACC G^GT[ACGT]ACC
BstPI 984 GGTGACC G^GT[ACGT]ACC
Cfr10I 190 GCCGGC [GA]^CCGG[CT]
DraII 756 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 460 CCACGG C^C[GA][CT]GG
EcoO109I 756 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 984 GGTGACC G^GT[ACGT]ACC
EcoRI 323 GAATTC G^AATTC
FauI 933 CCCGC CCCGC(4/6)
HaeII 130 GGCGCC [GA]GCGC^[CT]
KasI 130 GGCGCC G^GCGCC
MseI 595 TTAA T^TAA
NaeI 190 GCCGGC GCC^GGC
NarI 130 GGCGCC GG^CGCC
NciI 221 CCCGG CC^[GC]GG
NgoMIV 190 GCCGGC G^CCGGC
PaeR7I 1020 CTCGAG C^TCGAG
PflMI 535 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 277 CAGCTG CAG^CTG
SacI 268 GAGCTC GAGCT^C
SapI 1056 GCTCTTC GCTCTTC(1/4)
SfoI 130 GGCGCC GGC^GCC
SmlI 1020 CTCGAG C^T[CT][GA]AG
TfiI 545 GATTC G^A[AT]TC
Tru9I 595 TTAA T^TAA
Van91I 535 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 180 TCTAGA T^CTAGA
XhoI 1020 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 378 GATCC GGATC(4/5)
119 GGATC
AlwNI 583 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
951 CAGAAACTG
AvaI 231 CCCGAG C^[CT]CG[GA]G
1020 CTCGAG
BalI 341 TGGCCA TGG^CCA
1097 TGGCCA
BanII 268 GAGCTC G[GA]GC[CT]^C
843 GGGCTC
BfaI 50 CTAG C^TAG
181 CTAG
BmyI 268 GAGCTC G[AGT]GC[ACT]^C
843 GGGCTC
BsmAI 234 GAGAC GTCTC(1/5)
874 GAGAC
BsoBI 231 CCCGAG C^[CT]CG[GA]G
1020 CTCGAG
Bsp1286I 268 GAGCTC G[AGT]GC[ACT]^C
843 GGGCTC
BstXI 626 CCATGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
789 CCAATCCAGTGG
CfoI 131 GCGC GCG^C
1025 GCGC
DpnII 120 GATC ^GATC
378 GATC
EarI 1057 CTCTTC CTCTTC(1/4)
871 GAAGAG
HhaI 131 GCGC GCG^C
1025 GCGC
HinP1I 131 GCGC G^CGC
1025 GCGC
Ksp632I 1057 CTCTTC CTCTTC(1/4)
871 GAAGAG
MaeI 50 CTAG C^TAG
181 CTAG
MboI 120 GATC ^GATC
378 GATC
MluNI 341 TGGCCA TGG^CCA
1097 TGGCCA
MscI 341 TGGCCA TGG^CCA
1097 TGGCCA
NdeII 120 GATC ^GATC
378 GATC
NlaIII 144 CATG CATG^
627 CATG
PleI 800 GACTC GAGTC(4/5)
1018 GACTC
Sau3AI 120 GATC ^GATC
378 GATC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI BbvI BsaHI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI BstUI Cac8I Cfr13I
DdeI EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NlaIV
RsaI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I