(Circular) (Six-base) MAPSORT of: pFN21AB5451.seq Check: 1130 from: 1 to: 5821 pFN21AB5451 5821 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2051 2051 Size: 5821 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2474 3631 278 Size: 53 83 186 936 938 1157 2468 Fragments arranged by size: 2468 1157 938 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 3164 1937 Size: 208 1019 4594 Fragments arranged by size: 4594 1019 208 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 2550 3062 3884 5268 5536 1632 Size: 488 430 512 822 1384 268 1917 Fragments arranged by size: 1917 1384 822 512 488 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 3048 4720 5697 1203 Size: 1845 1672 977 1327 Fragments arranged by size: 1845 1672 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3107 1184 Size: 1923 3898 AlwNI CAG_nnn'CTG Cuts at: 2593 2961 3564 5136 2593 Size: 368 603 1572 3278 Fragments arranged by size: 3278 1572 603 368 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 2181 2328 2518 2816 3130 3356 3508 5617 Size: 688 147 190 298 314 226 152 2109 Cuts at: 5617 1493 Size: 1697 Fragments arranged by size: 2109 1697 688 314 298 226 190 152 147 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2236 3025 3146 1870 Size: 73 293 789 121 4545 Fragments arranged by size: 4545 789 293 121 73 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 3142 4597 618 Size: 325 217 627 348 1007 1455 1842 Fragments arranged by size: 1842 1455 1007 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 2277 2852 3140 1391 Size: 886 575 288 4072 Fragments arranged by size: 4072 886 575 288 BbeI G_GCGC'C Cuts at: 2139 2139 Size: 5821 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 928 Size: 971 657 4193 Fragments arranged by size: 4193 971 657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2625 4432 136 Size: 107 122 71 631 605 953 1807 1525 Fragments arranged by size: 1807 1525 953 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 3882 5038 1790 Size: 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 BmgBI CAC'GTC Cuts at: 1318 2424 1318 Size: 1106 4715 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3552 4510 442 Size: 416 53 83 399 24 2135 958 1753 Fragments arranged by size: 2135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4481 1239 Size: 3242 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2873 4484 882 Size: 617 1374 1611 2219 Fragments arranged by size: 2219 1611 1374 617 BsaAI yAC'GTr Cuts at: 493 1204 3091 3612 493 Size: 711 1887 521 2702 Fragments arranged by size: 2702 1887 711 521 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2316 2471 3114 Size: 53 83 186 936 603 180 155 643 Cuts at: 3114 3628 4010 4645 275 Size: 514 382 635 1451 Fragments arranged by size: 1451 936 643 635 603 514 382 186 180 155 83 53 BsaWI w'CCGG_w Cuts at: 2121 4254 4652 4926 5073 5484 2121 Size: 2133 398 274 147 411 2458 Fragments arranged by size: 2458 2133 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 1186 Size: 1099 4722 BseYI C'CCAG_C Cuts at: 2711 2948 5024 5520 2711 Size: 237 2076 496 3012 Fragments arranged by size: 3012 2076 496 237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3113 1163 Size: 573 1377 3871 Fragments arranged by size: 3871 1377 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 3882 3967 5038 2277 Size: 1605 85 1071 3060 Fragments arranged by size: 3060 1605 1071 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2852 3140 3882 3967 5038 1391 Size: 399 487 575 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 487 399 288 85 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2051 3176 844 Size: 1207 1125 3489 Fragments arranged by size: 3489 1207 1125 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2206 4316 4490 65 Size: 1183 958 2110 174 1396 Fragments arranged by size: 2110 1396 1183 958 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2195 4465 4652 1700 Size: 110 385 2270 187 2869 Fragments arranged by size: 2869 2270 385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2961 794 Size: 2167 3654 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2989 1184 Size: 1805 4016 BstXI CCAn_nnnn'nTGG Cuts at: 2638 2801 2638 Size: 163 5658 BstYI r'GATC_y Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816 Size: 1179 367 385 17 1452 86 12 346 Cuts at: 5816 1972 Size: 1977 Fragments arranged by size: 1977 1452 1179 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2465 513 Size: 552 1400 3869 Fragments arranged by size: 3869 1400 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2287 487 Size: 683 56 1061 4021 Fragments arranged by size: 4021 1061 683 56 BtsI GCAGTG_nn' Cuts at: 3337 4131 4151 3337 Size: 794 20 5007 Fragments arranged by size: 5007 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2346 3102 Size: 54 1425 50 275 18 363 153 756 Cuts at: 3102 3268 4157 5786 8 Size: 166 889 1629 43 Fragments arranged by size: 1629 1425 889 756 363 275 166 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3068 3751 1353 Size: 1518 197 683 3423 Fragments arranged by size: 3423 1518 683 197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2921 4408 4782 4928 387 Size: 1064 138 1332 1487 374 146 1280 Fragments arranged by size: 1487 1332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2550 3062 3884 4481 5268 5536 Size: 393 488 430 512 822 597 787 268 Cuts at: 5536 1239 Size: 1524 Fragments arranged by size: 1524 822 787 597 512 488 430 393 268 EcoO109I rG'GnC_Cy Cuts at: 2762 2762 Size: 5821 EcoRI G'AATT_C Cuts at: 2328 3130 3508 2328 Size: 802 378 4641 Fragments arranged by size: 4641 802 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2888 2614 Size: 274 5547 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 2139 4968 1678 Size: 461 2829 2531 Fragments arranged by size: 2829 2531 461 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2286 2318 4462 Size: 32 135 32 508 32 174 32 2144 Cuts at: 4462 4494 4536 4568 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2144 508 174 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3165 3411 669 Size: 988 281 1227 246 3079 Fragments arranged by size: 3079 1227 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2135 2135 Size: 5821 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 2877 4935 5119 1305 Size: 1050 522 2058 184 2007 Fragments arranged by size: 2058 2007 1050 522 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2348 3104 10 Size: 54 1425 343 516 756 2727 Fragments arranged by size: 2727 1425 756 516 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2636 3018 3089 3779 4138 4297 518 Size: 648 1470 382 71 690 359 159 2042 Fragments arranged by size: 2042 1470 690 648 382 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2177 2284 2903 2952 3237 3914 5062 5307 Size: 571 107 619 49 285 677 1148 245 Cuts at: 5307 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 619 571 285 245 107 49 NaeI GCC'GGC Cuts at: 2197 2197 Size: 5821 NarI GG'CG_CC Cuts at: 2136 2136 Size: 5821 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2195 2195 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 3179 4724 1132 Size: 97 1950 1545 2229 Fragments arranged by size: 2229 1950 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2546 1108 Size: 1438 4383 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PspXI vC'TCGA_Gb Cuts at: 3025 3025 Size: 5821 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2284 2284 Size: 5821 SacI G_AGCT'C Cuts at: 2277 2277 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 3068 3068 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 3169 4304 4985 5176 826 Size: 219 2124 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2137 2137 Size: 5821 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3025 3925 4826 5088 820 Size: 197 926 1082 900 901 262 1553 Fragments arranged by size: 1553 1082 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2451 2700 2758 3117 3260 513 Size: 552 1386 249 58 359 143 3074 Fragments arranged by size: 3074 1386 552 359 249 143 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2670 3848 3865 4018 4203 4648 325 Size: 1255 1090 1178 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1178 1090 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3051 4067 5779 Size: 274 80 33 51 1443 1074 1016 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1443 1074 1016 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 2185 3157 2185 Size: 972 4849 XhoI C'TCGA_G Cuts at: 1943 3025 1943 Size: 1082 4739 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2472 3629 276 Size: 53 83 186 936 938 1157 2468 Fragments arranged by size: 2468 1157 938 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI