(Circular) (Six-base) MAPSORT of: pFN21AB5297.seq Check: 5432 from: 1 to: 5605 pFN21AB5297 5605 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 2948 1937 Size: 343 668 4594 Fragments arranged by size: 4594 668 343 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3668 5052 5320 1632 Size: 2036 1384 268 1917 Fragments arranged by size: 2036 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2530 2645 4504 5481 1203 Size: 1327 115 1859 977 1327 Fragments arranged by size: 1859 1327 1327 977 115 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 2135 3348 4920 2135 Size: 1213 1572 2820 Fragments arranged by size: 2820 1572 1213 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2009 2168 2914 3140 3292 5401 1493 Size: 516 159 746 226 152 2109 1697 Fragments arranged by size: 2109 1697 746 516 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2930 1870 Size: 73 987 4545 Fragments arranged by size: 4545 987 73 BamHI G'GATC_C Cuts at: 2817 2935 3302 2817 Size: 118 367 5120 Fragments arranged by size: 5120 367 118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 2926 4381 618 Size: 325 217 627 468 671 1455 1842 Fragments arranged by size: 1842 1455 671 627 468 325 217 BanII G_rGCy'C Cuts at: 1391 2924 1391 Size: 1533 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BfrBI ATG'CAT Cuts at: 2746 2746 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 3666 4822 1790 Size: 1876 1156 2573 Fragments arranged by size: 2573 1876 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2261 3336 4294 Size: 416 53 83 399 24 844 1075 958 Cuts at: 4294 442 Size: 1753 Fragments arranged by size: 1753 1075 958 844 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 2652 4265 1239 Size: 1004 409 1613 2579 Fragments arranged by size: 2579 1613 1004 409 Bpu10I CC'TnA_GC Cuts at: 2826 2826 Size: 5605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4268 882 Size: 617 2769 2219 Fragments arranged by size: 2769 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2107 1186 Size: 921 4684 BseYI C'CCAG_C Cuts at: 2135 4808 5304 2135 Size: 2673 496 2436 Fragments arranged by size: 2673 2436 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5032 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3666 3751 4822 3666 Size: 85 1071 4449 Fragments arranged by size: 4449 1071 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2924 3666 3751 4822 1391 Size: 399 1134 742 85 1071 2174 Fragments arranged by size: 2174 1134 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2484 4100 4274 65 Size: 1183 1236 1616 174 1396 Fragments arranged by size: 1616 1396 1236 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 4249 4436 1700 Size: 110 298 2141 187 2869 Fragments arranged by size: 2869 2141 298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2404 2817 2935 3302 3687 3704 5156 5242 Size: 432 413 118 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 432 413 385 367 346 118 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2721 2721 Size: 5605 BtgI C'CryG_G Cuts at: 513 1065 2494 513 Size: 552 1429 3624 Fragments arranged by size: 3624 1429 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2847 2909 2847 Size: 62 5543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2497 3052 3941 Size: 54 1425 50 275 18 667 555 889 Cuts at: 3941 5570 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 667 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3535 1353 Size: 2182 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2652 3668 4265 5052 5320 1239 Size: 393 611 409 1016 597 787 268 1524 Fragments arranged by size: 1524 1016 787 611 597 409 393 268 EcoO109I rG'GnC_Cy Cuts at: 2705 2705 Size: 5605 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4246 4278 4320 4352 1373 Size: 32 135 32 2674 32 42 32 2626 Fragments arranged by size: 2674 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 2075 748 Size: 1327 4278 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 4719 4903 1305 Size: 1258 2156 184 2007 Fragments arranged by size: 2156 2007 1258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2499 10 Size: 54 1425 343 667 3116 Fragments arranged by size: 3116 1425 667 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2650 3563 3922 4081 518 Size: 648 1484 913 359 159 2042 Fragments arranged by size: 2042 1484 913 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 2525 3021 3698 4846 5091 1606 Size: 628 291 496 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 628 496 291 245 NcoI C'CATG_G Cuts at: 513 1065 2494 513 Size: 552 1429 3624 Fragments arranged by size: 3624 1429 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NsiI A_TGCA'T Cuts at: 2748 2748 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2534 2649 2963 4508 1132 Size: 97 1305 115 314 1545 2229 Fragments arranged by size: 2229 1545 1305 314 115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 2530 2645 4504 2530 Size: 115 1859 3631 Fragments arranged by size: 3631 1859 115 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2705 2705 Size: 5605 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2288 2957 830 Size: 1458 669 3478 Fragments arranged by size: 3478 1458 669 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2234 2525 2234 Size: 291 5314 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SanDI GG'GwC_CC Cuts at: 2705 2705 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2284 2790 2953 4088 4769 4960 826 Size: 219 1239 506 163 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 681 506 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 2108 1700 Size: 408 5197 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2259 2494 3044 513 Size: 552 1194 235 550 3074 Fragments arranged by size: 3074 1194 552 550 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 2527 3632 3649 3802 3987 4432 Size: 1255 663 284 1105 17 153 185 445 Cuts at: 4432 325 Size: 1498 Fragments arranged by size: 1498 1255 1105 663 445 284 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2437 2693 3851 Size: 274 80 33 51 1443 460 256 1158 Cuts at: 3851 5563 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1158 460 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI