Restriction map for
pFN21AB5297
(Linear) (Six-base) MAPSORT of: KIBB5297.seq Check: 7909 from: 1 to: 900
KIBB5297.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 275 900
Size: 275 625
AflIII A'CryG_T
Cuts at: 0 525 640 900
Size: 525 115 260
Fragments arranged by size:
525 260 115
AlwNI CAG_nnn'CTG
Cuts at: 0 130 900
Size: 130 770
ApoI r'AATT_y
Cuts at: 0 4 163 900
Size: 4 159 737
Fragments arranged by size:
737 159 4
BamHI G'GATC_C
Cuts at: 0 812 900
Size: 812 88
BanI G'GyrC_C
Cuts at: 0 250 900
Size: 250 650
BfrBI ATG'CAT
Cuts at: 0 741 900
Size: 741 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 256 900
Size: 256 644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 647 900
Size: 238 409 253
Fragments arranged by size:
409 253 238
Bpu10I CC'TnA_GC
Cuts at: 0 821 900
Size: 821 79
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 900
Size: 102 798
BseYI C'CCAG_C
Cuts at: 0 130 900
Size: 130 770
BsrDI GCAATG_nn'
Cuts at: 0 479 900
Size: 479 421
BsrFI r'CCGG_y
Cuts at: 0 103 900
Size: 103 797
BstYI r'GATC_y
Cuts at: 0 399 812 900
Size: 399 413 88
Fragments arranged by size:
413 399 88
Bsu36I CC'TnA_GG
Cuts at: 0 716 900
Size: 716 184
BtgI C'CryG_G
Cuts at: 0 489 900
Size: 489 411
DraI TTT'AAA
Cuts at: 0 842 900
Size: 842 58
EaeI y'GGCC_r
Cuts at: 0 492 900
Size: 492 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 647 900
Size: 238 409 253
Fragments arranged by size:
409 253 238
EcoO109I rG'GnC_Cy
Cuts at: 0 700 900
Size: 700 200
HindIII A'AGCT_T
Cuts at: 0 70 900
Size: 70 830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 900
Size: 558 342
MscI TGG'CCA
Cuts at: 0 494 900
Size: 494 406
MslI CAynn'nnrTG
Cuts at: 0 645 900
Size: 645 255
MspA1I CmG'CkG
Cuts at: 0 229 520 900
Size: 229 291 380
Fragments arranged by size:
380 291 229
NcoI C'CATG_G
Cuts at: 0 489 900
Size: 489 411
NsiI A_TGCA'T
Cuts at: 0 743 900
Size: 743 157
NspI r_CATG'y
Cuts at: 0 529 644 900
Size: 529 115 256
Fragments arranged by size:
529 256 115
PciI A'CATG_T
Cuts at: 0 525 640 900
Size: 525 115 260
Fragments arranged by size:
525 260 115
PpuMI rG'GwC_Cy
Cuts at: 0 700 900
Size: 700 200
PstI C_TGCA'G
Cuts at: 0 283 900
Size: 283 617
PvuII CAG'CTG
Cuts at: 0 229 520 900
Size: 229 291 380
Fragments arranged by size:
380 291 229
SanDI GG'GwC_CC
Cuts at: 0 700 900
Size: 700 200
SfcI C'TryA_G
Cuts at: 0 279 785 900
Size: 279 506 115
Fragments arranged by size:
506 279 115
SgrAI Cr'CCGG_yG
Cuts at: 0 103 900
Size: 103 797
StyI C'CwwG_G
Cuts at: 0 254 489 900
Size: 254 235 411
Fragments arranged by size:
411 254 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 522 900
Size: 238 284 378
Fragments arranged by size:
378 284 238
TatI w'GTAC_w
Cuts at: 0 432 688 900
Size: 432 256 212
Fragments arranged by size:
432 256 212
Enzymes that do cut:
AccI AflIII AlwNI ApoI BamHI BanI BfrBI BmrI
BpmI Bpu10I BseRI BseYI BsrDI BsrFI BstYI Bsu36I
BtgI DraI EaeI Eco57MI EcoO109I HindIII MmeI MscI
MslI MspA1I NcoI NsiI NspI PciI PpuMI PstI
PvuII SanDI SfcI SgrAI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI