Restriction map for
pFN21AE2122
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BbeI BbrPI BbvCI BciVI BfrI
BlnI BsaAI BsaI BsiWI BspLU11I
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoT22I
FauI FseI HindIII HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1447 GACGTC GACGT^C
AccIII 56 TCCGGA T^CCGGA
AclI 1795 AACGTT AA^CGTT
AcyI 1447 GACGTC G[GA]^CG[CT]C
Aor51HI 942 AGCGCT AGC^GCT
AseI 2224 ATTAAT AT^TAAT
AsnI 2224 ATTAAT AT^TAAT
Asp700I 1818 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2413 GAGCTC G[AT]GC[AT]^C
AviII 287 TGCGCA TGC^GCA
BamHI 594 GGATCC G^GATCC
BanI 361 GGCACC G^G[CT][GA]CC
BcgI 822 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 306 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1447 GACGTC G[GA]^CG[CT]C
BseAI 56 TCCGGA T^CCGGA
BsiHKAI 2413 GAGCTC G[AT]GC[AT]^C
BspDI 1828 ATCGAT AT^CGAT
BspEI 56 TCCGGA T^CCGGA
ClaI 1828 ATCGAT AT^CGAT
DraI 526 TTTAAA TTT^AAA
EagI 898 CGGCCG C^GGCCG
EclXI 898 CGGCCG C^GGCCG
Eco47III 942 AGCGCT AGC^GCT
Eco52I 898 CGGCCG C^GGCCG
EcoRV 2128 GATATC GAT^ATC
FspI 287 TGCGCA TGC^GCA
HgaI 60 GACGC GACGC(5/10)
Hin1I 1447 GACGTC G[GA]^CG[CT]C
KspI 395 CCGCGG CCGC^GG
MamI 306 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 56 TCCGGA T^CCGGA
NaeI 754 GCCGGC GCC^GGC
NcoI 365 CCATGG C^CATGG
NgoMIV 754 GCCGGC G^CCGGC
PaeR7I 1643 CTCGAG C^TCGAG
PshBI 2224 ATTAAT AT^TAAT
Psp1406I 1795 AACGTT AA^CGTT
PvuII 2546 CAGCTG CAG^CTG
SacI 2413 GAGCTC GAGCT^C
SacII 395 CCGCGG CCGC^GG
SphI 1269 GCATGC GCATG^C
XcmI 2526 CCAGAAGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1643 CTCGAG C^TCGAG
XmnI 1818 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AvaI 626 CCCGAG C^[CT]CG[GA]G
1643 CTCGAG
BalI 2102 TGGCCA TGG^CCA
2141 TGGCCA
BanII 632 GGGCTC G[GA]GC[CT]^C
2413 GAGCTC
BbsI 575 GAAGAC GAAGAC(2/6)
1450 GTCTTC
BcnI 1412 CCCGG CC^[GC]GG
1635 CCGGG
BglII 249 AGATCT A^GATCT
1122 AGATCT
BlpI 1035 GCTCAGC GC^T[ACGT]AGC
2050 GCTGAGC
BmyI 632 GGGCTC G[AGT]GC[ACT]^C
2413 GAGCTC
Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC
2050 GCTGAGC
BpuAI 575 GAAGAC GAAGAC(2/6)
1450 GTCTTC
BsaWI 56 TCCGGA [AT]^CCGG[AT]
1610 ACCGGA
BsgI 963 CTGCAC GTGCAG(16/14)
2395 GTGCAG
BsiEI 898 CGGCCG CG[GA][CT]^CG
975 CGACCG
BsmBI 301 GAGACG CGTCTC(1/5)
971 GAGACG
BsmI 645 GAATGC GAATGC(1/-1)
2475 GAATGC
BsoBI 626 CCCGAG C^[CT]CG[GA]G
1643 CTCGAG
Bsp1286I 632 GGGCTC G[AGT]GC[ACT]^C
2413 GAGCTC
Bsp1407I 659 TGTACA T^GTACA
2676 TGTACA
BspHI 437 TCATGA T^CATGA
2162 TCATGA
BsrBI 1394 CCGCTC CCGCTC(-3/-3)
1221 GAGCGG
BsrFI 754 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
BsrGI 659 TGTACA T^GTACA
2676 TGTACA
BssSI 2636 CACGAG CACGAG(-5/-1)
2276 CTCGTG
BstXI 41 CCAAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1274 CCAACAAACTGG
CelII 1035 GCTCAGC GC^T[ACGT]AGC
2050 GCTGAGC
Cfr10I 754 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
EcoNI 213 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1581 CCTCCAAGAGG
EcoRI 2626 GAATTC G^AATTC
2692 GAATTC
EcoT14I 365 CCATGG C^C[AT][AT]GG
800 CCAAGG
HaeII 942 AGCGCT [GA]GCGC^[CT]
1020 GGCGCT
MluNI 2102 TGGCCA TGG^CCA
2141 TGGCCA
MscI 2102 TGGCCA TGG^CCA
2141 TGGCCA
MslI 688 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1509 CACCTCAGTG
NciI 1412 CCCGG CC^[GC]GG
1635 CCGGG
NspI 1269 GCATGC [GA]CATG^[CT]
2308 GCATGT
PstI 1616 CTGCAG CTGCA^G
2373 CTGCAG
RcaI 437 TCATGA T^CATGA
2162 TCATGA
SspBI 659 TGTACA T^GTACA
2676 TGTACA
StyI 365 CCATGG C^C[AT][AT]GG
800 CCAAGG
Tsp45I 103 GTGAC ^GT[GC]AC
400 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbvI
BclI BfaI BglI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII