Restriction map for pFN21AE2122
 (Linear) (Six-base) MAPSORT of: KIEE2122.seq  Check: 7118  from: 1  to: 2715



KIEE2122.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2046    2715 
   Size:       2046     669
 
AatII G_ACGT'C

Cuts at:      0    1451    2715 
   Size:       1451    1264
 
AclI AA'CG_TT

Cuts at:      0    1796    2715 
   Size:       1796     919
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     658    2642    2715 
   Size:        610      48    1984      73

  Fragments arranged by size: 

                1984     610      73      48
 
AfeI AGC'GCT

Cuts at:      0     944    2715 
   Size:        944    1771
 
AleI CACnn'nnGTG

Cuts at:      0    1513    2715 
   Size:       1513    1202
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     530    1027    2715 
   Size:        530     497    1688

  Fragments arranged by size: 

                1688     530     497
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1807    1839    2715 
   Size:       1807      32     876

  Fragments arranged by size: 

                1807     876      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     588    1032    2403    2548    2715 
   Size:        588     444    1371     145     167

  Fragments arranged by size: 

                1371     588     444     167     145
 
ApoI r'AATT_y

Cuts at:      0     207    2626    2692    2715 
   Size:        207    2419      66      23

  Fragments arranged by size: 

                2419     207      66      23
 
AseI AT'TA_AT

Cuts at:      0    2225    2715 
   Size:       2225     490
 
AvaI C'yCGr_G

Cuts at:      0     626    1643    2715 
   Size:        626    1017    1072

  Fragments arranged by size: 

                1072    1017     626
 
BamHI G'GATC_C

Cuts at:      0     594    2715 
   Size:        594    2121
 
BanI G'GyrC_C

Cuts at:      0     361    2715 
   Size:        361    2354
 
BanII G_rGCy'C

Cuts at:      0     636    2417    2715 
   Size:        636    1781     298

  Fragments arranged by size: 

                1781     636     298
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     582    1443    2715 
   Size:        582     861    1272

  Fragments arranged by size: 

                1272     861     582
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     811     845    2715 
   Size:        811      34    1870

  Fragments arranged by size: 

                1870     811      34
 
BclI T'GATC_A

Cuts at:      0    1886    2159    2289    2715 
   Size:       1886     273     130     426

  Fragments arranged by size: 

                1886     426     273     130
 
BglI GCCn_nnn'nGGC

Cuts at:      0     631     812    1300    2014    2715 
   Size:        631     181     488     714     701

  Fragments arranged by size: 

                 714     701     631     488     181
 
BglII A'GATC_T

Cuts at:      0     249    1122    2715 
   Size:        249     873    1593

  Fragments arranged by size: 

                1593     873     249
 
BlpI GC'TnA_GC

Cuts at:      0    1036    2051    2715 
   Size:       1036    1015     664

  Fragments arranged by size: 

                1036    1015     664
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1099    2431    2715 
   Size:       1099    1332     284

  Fragments arranged by size: 

                1332    1099     284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     928     940    1009    2570    2715 
   Size:        928      12      69    1561     145

  Fragments arranged by size: 

                1561     928     145      69      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     236    1433    2715 
   Size:        236    1197    1282

  Fragments arranged by size: 

                1282    1197     236
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656     758    2715 
   Size:        656     102    1957

  Fragments arranged by size: 

                1957     656     102
 
BsaBI GATnn'nnATC

Cuts at:      0     310    2715 
   Size:        310    2405
 
BsaHI Gr'CG_yC

Cuts at:      0    1448    2715 
   Size:       1448    1267
 
BsaWI w'CCGG_w

Cuts at:      0      56    1610    2715 
   Size:         56    1554    1105

  Fragments arranged by size: 

                1554    1105      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1807    1837    2715 
   Size:       1807      30     878

  Fragments arranged by size: 

                1807     878      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     309     925     948    1828    2047    2423    2715 
   Size:        309     616      23     880     219     376     292

  Fragments arranged by size: 

                 880     616     376     309     292     219      23
 
BseYI C'CCAG_C

Cuts at:      0    1687    2715 
   Size:       1687    1028
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     948    2416    2715 
   Size:        948    1468     299

  Fragments arranged by size: 

                1468     948     299
 
BsiEI CG_ry'CG

Cuts at:      0     901     978    2715 
   Size:        901      77    1737

  Fragments arranged by size: 

                1737     901      77
 
BsiHKAI G_wGCw'C

Cuts at:      0    2417    2715 
   Size:       2417     298
 
BsmI GAATG_Cn'

Cuts at:      0     651    2481    2715 
   Size:        651    1830     234

  Fragments arranged by size: 

                1830     651     234
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     295     965    2715 
   Size:        295     670    1750

  Fragments arranged by size: 

                1750     670     295
 
Bsp1286I G_dGCh'C

Cuts at:      0     636    2417    2715 
   Size:        636    1781     298

  Fragments arranged by size: 

                1781     636     298
 
BspEI T'CCGG_A

Cuts at:      0      56    2715 
   Size:         56    2659
 
BspHI T'CATG_A

Cuts at:      0     437    2162    2715 
   Size:        437    1725     553

  Fragments arranged by size: 

                1725     553     437
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1040    1464    2046    2242    2715 
   Size:       1040     424     582     196     473

  Fragments arranged by size: 

                1040     582     473     424     196
 
BsrBI CCG'CTC

Cuts at:      0    1223    1396    2715 
   Size:       1223     173    1319

  Fragments arranged by size: 

                1319    1223     173
 
BsrDI GCAATG_nn'

Cuts at:      0    1088    1985    2130    2708    2715 
   Size:       1088     897     145     578       7

  Fragments arranged by size: 

                1088     897     578     145       7
 
BsrFI r'CCGG_y

Cuts at:      0     754     977    2715 
   Size:        754     223    1738

  Fragments arranged by size: 

                1738     754     223
 
BsrGI T'GTAC_A

Cuts at:      0     659    2676    2715 
   Size:        659    2017      39

  Fragments arranged by size: 

                2017     659      39
 
BssSI C'ACGA_G

Cuts at:      0    2276    2636    2715 
   Size:       2276     360      79

  Fragments arranged by size: 

                2276     360      79
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48    1281    2715 
   Size:         48    1233    1434

  Fragments arranged by size: 

                1434    1233      48
 
BstYI r'GATC_y

Cuts at:      0     249     594    1077    1122    2715 
   Size:        249     345     483      45    1593

  Fragments arranged by size: 

                1593     483     345     249      45
 
BtgI C'CryG_G

Cuts at:      0     365     395    1496    2715 
   Size:        365      30    1101    1219

  Fragments arranged by size: 

                1219    1101     365      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     735    1607    2429    2715 
   Size:        735     872     822     286

  Fragments arranged by size: 

                 872     822     735     286
 
BtsI GCAGTG_nn'

Cuts at:      0    1154    2715 
   Size:       1154    1561
 
ClaI AT'CG_AT

Cuts at:      0    1829    2715 
   Size:       1829     886
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1344    1379    2715 
   Size:       1344      35    1336

  Fragments arranged by size: 

                1344    1336      35
 
DraI TTT'AAA

Cuts at:      0     528    2715 
   Size:        528    2187
 
EaeI y'GGCC_r

Cuts at:      0     898     950    1414    2102    2141    2715 
   Size:        898      52     464     688      39     574

  Fragments arranged by size: 

                 898     688     574     464      52      39
 
EagI C'GGCC_G

Cuts at:      0     898    2715 
   Size:        898    1817
 
EarI CTCTTCn'nnn_

Cuts at:      0     254     464    2602    2715 
   Size:        254     210    2138     113

  Fragments arranged by size: 

                2138     254     210     113
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     436     673    1012    2715 
   Size:        436     237     339    1703

  Fragments arranged by size: 

                1703     436     339     237
 
EcoICRI GAG'CTC

Cuts at:      0    2415    2715 
   Size:       2415     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     658     928     940    1009    2570    2642    2715 
   Size:        610      48     270      12      69    1561      72      73

  Fragments arranged by size: 

                1561     610     270      73      72      69      48      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     217    1585    2715 
   Size:        217    1368    1130

  Fragments arranged by size: 

                1368    1130     217
 
EcoO109I rG'GnC_Cy

Cuts at:      0      36     474    1029    1892    2715 
   Size:         36     438     555     863     823

  Fragments arranged by size: 

                 863     823     555     438      36
 
EcoRI G'AATT_C

Cuts at:      0    2626    2692    2715 
   Size:       2626      66      23

  Fragments arranged by size: 

                2626      66      23
 
EcoRV GAT'ATC

Cuts at:      0    2130    2715 
   Size:       2130     585
 
FspI TGC'GCA

Cuts at:      0     289    2715 
   Size:        289    2426
 
HaeII r_GCGC'y

Cuts at:      0     946    1024    2715 
   Size:        946      78    1691

  Fragments arranged by size: 

                1691     946      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     234     266     328     360    1147    1179    1495    1513 
   Size:        234      32      62      32     787      32     316      18

Cuts at:   1513    1527    1545    2055    2087    2568    2600    2715 
   Size:         14      18     510      32     481      32     115

  Fragments arranged by size: 

                 787     510     481     316     234     115      62      32
                  32      32      32      32      18      18      14
 
HincII GTy'rAC

Cuts at:      0     117    1487    2553    2715 
   Size:        117    1370    1066     162

  Fragments arranged by size: 

                1370    1066     162     117
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1340    1691    2715 
   Size:         62    1278     351    1024

  Fragments arranged by size: 

                1278    1024     351      62
 
MscI TGG'CCA

Cuts at:      0    2104    2143    2715 
   Size:       2104      39     572

  Fragments arranged by size: 

                2104     572      39
 
MslI CAynn'nnrTG

Cuts at:      0     692    1513    2715 
   Size:        692     821    1202

  Fragments arranged by size: 

                1202     821     692
 
MspA1I CmG'CkG

Cuts at:      0     397     935    2120    2548    2715 
   Size:        397     538    1185     428     167

  Fragments arranged by size: 

                1185     538     428     397     167
 
NaeI GCC'GGC

Cuts at:      0     756    2715 
   Size:        756    1959
 
NcoI C'CATG_G

Cuts at:      0     365    2715 
   Size:        365    2350
 
NgoMIV G'CCGG_C

Cuts at:      0     754    2715 
   Size:        754    1961
 
NspI r_CATG'y

Cuts at:      0    1273    2312    2715 
   Size:       1273    1039     403

  Fragments arranged by size: 

                1273    1039     403
 
PfoI T'CCnGG_A

Cuts at:      0     212     740     863    1025    1175    2715 
   Size:        212     528     123     162     150    1540

  Fragments arranged by size: 

                1540     528     212     162     150     123
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     877     909    1807    1839    2715 
   Size:        877      32     898      32     876

  Fragments arranged by size: 

                 898     877     876      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      36    1029    1892    2715 
   Size:         36     993     863     823

  Fragments arranged by size: 

                 993     863     823      36
 
PspXI vC'TCGA_Gb

Cuts at:      0    1643    2715 
   Size:       1643    1072
 
PstI C_TGCA'G

Cuts at:      0    1620    2377    2715 
   Size:       1620     757     338

  Fragments arranged by size: 

                1620     757     338
 
PvuII CAG'CTG

Cuts at:      0    2548    2715 
   Size:       2548     167
 
SacI G_AGCT'C

Cuts at:      0    2417    2715 
   Size:       2417     298
 
SacII CC_GC'GG

Cuts at:      0     398    2715 
   Size:        398    2317
 
SanDI GG'GwC_CC

Cuts at:      0      36    2715 
   Size:         36    2679
 
SfcI C'TryA_G

Cuts at:      0     188     275    1616    2373    2715 
   Size:        188      87    1341     757     342

  Fragments arranged by size: 

                1341     757     342     188      87
 
SmlI C'TyrA_G

Cuts at:      0     671     773    1643    2715 
   Size:        671     102     870    1072

  Fragments arranged by size: 

                1072     870     671     102
 
SphI G_CATG'C

Cuts at:      0    1273    2715 
   Size:       1273    1442
 
StyI C'CwwG_G

Cuts at:      0     365     800    2715 
   Size:        365     435    1915

  Fragments arranged by size: 

                1915     435     365
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     704    1372    2715 
   Size:        704     668    1343

  Fragments arranged by size: 

                1343     704     668
 
TatI w'GTAC_w

Cuts at:      0     174     659    1043    2136    2676    2715 
   Size:        174     485     384    1093     540      39

  Fragments arranged by size: 

                1093     540     485     384     174      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2533    2715 
   Size:       2533     182
 
XhoI C'TCGA_G

Cuts at:      0    1643    2715 
   Size:       1643    1072
 
XmnI GAAnn'nnTTC

Cuts at:      0    1822    2715 
   Size:       1822     893
 
ZraI GAC'GTC

Cuts at:      0    1449    2715 
   Size:       1449    1266
 

 Enzymes that do cut:

     AarI    AatII     AclI     AcuI     AfeI     AleI     AlfI     AloI
    AlwNI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI     BclI     BglI    BglII     BlpI     BplI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI     PfoI     PpiI    PpuMI    PspXI     PstI
    PvuII     SacI    SacII    SanDI     SfcI     SmlI     SphI     StyI
    TaqII     TatI     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I    AflII   AflIII     AgeI     AhdI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI    BfrBI
 Bme1580I    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsiWI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I   DraIII     DrdI     FalI
     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XmaI
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