(Linear) (Six-base) MAPSORT of: KIEE2122.seq Check: 7118 from: 1 to: 2715 KIEE2122.orf With 182 enzymes: SgfI * July 3, 2012 17:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2046 2715 Size: 2046 669 AatII G_ACGT'C Cuts at: 0 1451 2715 Size: 1451 1264 AclI AA'CG_TT Cuts at: 0 1796 2715 Size: 1796 919 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 610 658 2642 2715 Size: 610 48 1984 73 Fragments arranged by size: 1984 610 73 48 AfeI AGC'GCT Cuts at: 0 944 2715 Size: 944 1771 AleI CACnn'nnGTG Cuts at: 0 1513 2715 Size: 1513 1202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 530 1027 2715 Size: 530 497 1688 Fragments arranged by size: 1688 530 497 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1807 1839 2715 Size: 1807 32 876 Fragments arranged by size: 1807 876 32 AlwNI CAG_nnn'CTG Cuts at: 0 588 1032 2403 2548 2715 Size: 588 444 1371 145 167 Fragments arranged by size: 1371 588 444 167 145 ApoI r'AATT_y Cuts at: 0 207 2626 2692 2715 Size: 207 2419 66 23 Fragments arranged by size: 2419 207 66 23 AseI AT'TA_AT Cuts at: 0 2225 2715 Size: 2225 490 AvaI C'yCGr_G Cuts at: 0 626 1643 2715 Size: 626 1017 1072 Fragments arranged by size: 1072 1017 626 BamHI G'GATC_C Cuts at: 0 594 2715 Size: 594 2121 BanI G'GyrC_C Cuts at: 0 361 2715 Size: 361 2354 BanII G_rGCy'C Cuts at: 0 636 2417 2715 Size: 636 1781 298 Fragments arranged by size: 1781 636 298 BbsI GAAGACnn'nnnn_ Cuts at: 0 582 1443 2715 Size: 582 861 1272 Fragments arranged by size: 1272 861 582 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 811 845 2715 Size: 811 34 1870 Fragments arranged by size: 1870 811 34 BclI T'GATC_A Cuts at: 0 1886 2159 2289 2715 Size: 1886 273 130 426 Fragments arranged by size: 1886 426 273 130 BglI GCCn_nnn'nGGC Cuts at: 0 631 812 1300 2014 2715 Size: 631 181 488 714 701 Fragments arranged by size: 714 701 631 488 181 BglII A'GATC_T Cuts at: 0 249 1122 2715 Size: 249 873 1593 Fragments arranged by size: 1593 873 249 BlpI GC'TnA_GC Cuts at: 0 1036 2051 2715 Size: 1036 1015 664 Fragments arranged by size: 1036 1015 664 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1099 2431 2715 Size: 1099 1332 284 Fragments arranged by size: 1332 1099 284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 928 940 1009 2570 2715 Size: 928 12 69 1561 145 Fragments arranged by size: 1561 928 145 69 12 Bpu10I CC'TnA_GC Cuts at: 0 236 1433 2715 Size: 236 1197 1282 Fragments arranged by size: 1282 1197 236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 656 758 2715 Size: 656 102 1957 Fragments arranged by size: 1957 656 102 BsaBI GATnn'nnATC Cuts at: 0 310 2715 Size: 310 2405 BsaHI Gr'CG_yC Cuts at: 0 1448 2715 Size: 1448 1267 BsaWI w'CCGG_w Cuts at: 0 56 1610 2715 Size: 56 1554 1105 Fragments arranged by size: 1554 1105 56 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1807 1837 2715 Size: 1807 30 878 Fragments arranged by size: 1807 878 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 309 925 948 1828 2047 2423 2715 Size: 309 616 23 880 219 376 292 Fragments arranged by size: 880 616 376 309 292 219 23 BseYI C'CCAG_C Cuts at: 0 1687 2715 Size: 1687 1028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 948 2416 2715 Size: 948 1468 299 Fragments arranged by size: 1468 948 299 BsiEI CG_ry'CG Cuts at: 0 901 978 2715 Size: 901 77 1737 Fragments arranged by size: 1737 901 77 BsiHKAI G_wGCw'C Cuts at: 0 2417 2715 Size: 2417 298 BsmI GAATG_Cn' Cuts at: 0 651 2481 2715 Size: 651 1830 234 Fragments arranged by size: 1830 651 234 BsmBI CGTCTCn'nnnn_ Cuts at: 0 295 965 2715 Size: 295 670 1750 Fragments arranged by size: 1750 670 295 Bsp1286I G_dGCh'C Cuts at: 0 636 2417 2715 Size: 636 1781 298 Fragments arranged by size: 1781 636 298 BspEI T'CCGG_A Cuts at: 0 56 2715 Size: 56 2659 BspHI T'CATG_A Cuts at: 0 437 2162 2715 Size: 437 1725 553 Fragments arranged by size: 1725 553 437 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1040 1464 2046 2242 2715 Size: 1040 424 582 196 473 Fragments arranged by size: 1040 582 473 424 196 BsrBI CCG'CTC Cuts at: 0 1223 1396 2715 Size: 1223 173 1319 Fragments arranged by size: 1319 1223 173 BsrDI GCAATG_nn' Cuts at: 0 1088 1985 2130 2708 2715 Size: 1088 897 145 578 7 Fragments arranged by size: 1088 897 578 145 7 BsrFI r'CCGG_y Cuts at: 0 754 977 2715 Size: 754 223 1738 Fragments arranged by size: 1738 754 223 BsrGI T'GTAC_A Cuts at: 0 659 2676 2715 Size: 659 2017 39 Fragments arranged by size: 2017 659 39 BssSI C'ACGA_G Cuts at: 0 2276 2636 2715 Size: 2276 360 79 Fragments arranged by size: 2276 360 79 BstXI CCAn_nnnn'nTGG Cuts at: 0 48 1281 2715 Size: 48 1233 1434 Fragments arranged by size: 1434 1233 48 BstYI r'GATC_y Cuts at: 0 249 594 1077 1122 2715 Size: 249 345 483 45 1593 Fragments arranged by size: 1593 483 345 249 45 BtgI C'CryG_G Cuts at: 0 365 395 1496 2715 Size: 365 30 1101 1219 Fragments arranged by size: 1219 1101 365 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 735 1607 2429 2715 Size: 735 872 822 286 Fragments arranged by size: 872 822 735 286 BtsI GCAGTG_nn' Cuts at: 0 1154 2715 Size: 1154 1561 ClaI AT'CG_AT Cuts at: 0 1829 2715 Size: 1829 886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1344 1379 2715 Size: 1344 35 1336 Fragments arranged by size: 1344 1336 35 DraI TTT'AAA Cuts at: 0 528 2715 Size: 528 2187 EaeI y'GGCC_r Cuts at: 0 898 950 1414 2102 2141 2715 Size: 898 52 464 688 39 574 Fragments arranged by size: 898 688 574 464 52 39 EagI C'GGCC_G Cuts at: 0 898 2715 Size: 898 1817 EarI CTCTTCn'nnn_ Cuts at: 0 254 464 2602 2715 Size: 254 210 2138 113 Fragments arranged by size: 2138 254 210 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 436 673 1012 2715 Size: 436 237 339 1703 Fragments arranged by size: 1703 436 339 237 EcoICRI GAG'CTC Cuts at: 0 2415 2715 Size: 2415 300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 610 658 928 940 1009 2570 2642 2715 Size: 610 48 270 12 69 1561 72 73 Fragments arranged by size: 1561 610 270 73 72 69 48 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 217 1585 2715 Size: 217 1368 1130 Fragments arranged by size: 1368 1130 217 EcoO109I rG'GnC_Cy Cuts at: 0 36 474 1029 1892 2715 Size: 36 438 555 863 823 Fragments arranged by size: 863 823 555 438 36 EcoRI G'AATT_C Cuts at: 0 2626 2692 2715 Size: 2626 66 23 Fragments arranged by size: 2626 66 23 EcoRV GAT'ATC Cuts at: 0 2130 2715 Size: 2130 585 FspI TGC'GCA Cuts at: 0 289 2715 Size: 289 2426 HaeII r_GCGC'y Cuts at: 0 946 1024 2715 Size: 946 78 1691 Fragments arranged by size: 1691 946 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 234 266 328 360 1147 1179 1495 1513 Size: 234 32 62 32 787 32 316 18 Cuts at: 1513 1527 1545 2055 2087 2568 2600 2715 Size: 14 18 510 32 481 32 115 Fragments arranged by size: 787 510 481 316 234 115 62 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 0 117 1487 2553 2715 Size: 117 1370 1066 162 Fragments arranged by size: 1370 1066 162 117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 62 1340 1691 2715 Size: 62 1278 351 1024 Fragments arranged by size: 1278 1024 351 62 MscI TGG'CCA Cuts at: 0 2104 2143 2715 Size: 2104 39 572 Fragments arranged by size: 2104 572 39 MslI CAynn'nnrTG Cuts at: 0 692 1513 2715 Size: 692 821 1202 Fragments arranged by size: 1202 821 692 MspA1I CmG'CkG Cuts at: 0 397 935 2120 2548 2715 Size: 397 538 1185 428 167 Fragments arranged by size: 1185 538 428 397 167 NaeI GCC'GGC Cuts at: 0 756 2715 Size: 756 1959 NcoI C'CATG_G Cuts at: 0 365 2715 Size: 365 2350 NgoMIV G'CCGG_C Cuts at: 0 754 2715 Size: 754 1961 NspI r_CATG'y Cuts at: 0 1273 2312 2715 Size: 1273 1039 403 Fragments arranged by size: 1273 1039 403 PfoI T'CCnGG_A Cuts at: 0 212 740 863 1025 1175 2715 Size: 212 528 123 162 150 1540 Fragments arranged by size: 1540 528 212 162 150 123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 877 909 1807 1839 2715 Size: 877 32 898 32 876 Fragments arranged by size: 898 877 876 32 32 PpuMI rG'GwC_Cy Cuts at: 0 36 1029 1892 2715 Size: 36 993 863 823 Fragments arranged by size: 993 863 823 36 PspXI vC'TCGA_Gb Cuts at: 0 1643 2715 Size: 1643 1072 PstI C_TGCA'G Cuts at: 0 1620 2377 2715 Size: 1620 757 338 Fragments arranged by size: 1620 757 338 PvuII CAG'CTG Cuts at: 0 2548 2715 Size: 2548 167 SacI G_AGCT'C Cuts at: 0 2417 2715 Size: 2417 298 SacII CC_GC'GG Cuts at: 0 398 2715 Size: 398 2317 SanDI GG'GwC_CC Cuts at: 0 36 2715 Size: 36 2679 SfcI C'TryA_G Cuts at: 0 188 275 1616 2373 2715 Size: 188 87 1341 757 342 Fragments arranged by size: 1341 757 342 188 87 SmlI C'TyrA_G Cuts at: 0 671 773 1643 2715 Size: 671 102 870 1072 Fragments arranged by size: 1072 870 671 102 SphI G_CATG'C Cuts at: 0 1273 2715 Size: 1273 1442 StyI C'CwwG_G Cuts at: 0 365 800 2715 Size: 365 435 1915 Fragments arranged by size: 1915 435 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 704 1372 2715 Size: 704 668 1343 Fragments arranged by size: 1343 704 668 TatI w'GTAC_w Cuts at: 0 174 659 1043 2136 2676 2715 Size: 174 485 384 1093 540 39 Fragments arranged by size: 1093 540 485 384 174 39 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2533 2715 Size: 2533 182 XhoI C'TCGA_G Cuts at: 0 1643 2715 Size: 1643 1072 XmnI GAAnn'nnTTC Cuts at: 0 1822 2715 Size: 1822 893 ZraI GAC'GTC Cuts at: 0 1449 2715 Size: 1449 1266 Enzymes that do cut: AarI AatII AclI AcuI AfeI AleI AlfI AloI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BcgI BclI BglI BglII BlpI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PfoI PpiI PpuMI PspXI PstI PvuII SacI SacII SanDI SfcI SmlI SphI StyI TaqII TatI XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AflII AflIII AgeI AhdI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsiWI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I DraIII DrdI FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XmaI