################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AviII AvrII BamHI BanI BbeI BbvCI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsmI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AflIII 938 ACACGT A^C[GA][CT]GT AhdI 1021 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 402 GGATC GGATC(4/5) AspEI 1021 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 775 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 485 CCCGGG C^[CT]CG[GA]G BbrPI 187 CACGTG CAC^GTG BcgI 1023 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1015 CTAG C^TAG BlpI 4 GCTGAGC GC^T[ACGT]AGC BpmI 302 CTCCAG CTGGAG(16/14) Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BsaAI 187 CACGTG [CT]AC^GT[GA] BseRI 966 CTCCTC GAGGAG(10/8) BsiWI 50 CGTACG C^GTACG BsmBI 941 CGTCTC CGTCTC(1/5) BsoBI 485 CCCGGG C^[CT]CG[GA]G Bsp1407I 911 TGTACA T^GTACA BsrDI 959 GCAATG GCAATG(2/0) BsrFI 973 ACCGGC [GA]^CCGG[CT] BsrGI 911 TGTACA T^GTACA BstBI 22 TTCGAA TT^CGAA BstEII 684 GGTGACC G^GT[ACGT]ACC BstF5I 714 CATCC GGATG(2/0) BstPI 684 GGTGACC G^GT[ACGT]ACC CelII 4 GCTGAGC GC^T[ACGT]AGC Cfr10I 973 ACCGGC [GA]^CCGG[CT] DraII 796 GGGTCCC [GA]G^G[ACGT]CC[CT] Eam1105I 1021 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 796 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 684 GGTGACC G^GT[ACGT]ACC EcoT14I 251 CCAAGG C^C[AT][AT]GG FokI 714 CATCC GGATG(9/13) HgaI 118 GACGC GACGC(5/10) HincII 670 GTTGAC GT[CT]^[GA]AC HindII 670 GTTGAC GT[CT]^[GA]AC HindIII 760 AAGCTT A^AGCTT KspI 286 CCGCGG CCGC^GG MaeI 1015 CTAG C^TAG MslI 981 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 1014 GCTAGC G^CTAGC NspV 22 TTCGAA TT^CGAA PflFI 775 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 727 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 187 CACGTG CAC^GTG PmlI 187 CACGTG CAC^GTG PpuMI 796 GGGTCCC [GA]G^G[AT]CC[CT] PstI 553 CTGCAG CTGCA^G SacII 286 CCGCGG CCGC^GG SfiI 307 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 22 TTCGAA TT^CGAA SmaI 485 CCCGGG CCC^GGG SspBI 911 TGTACA T^GTACA StyI 251 CCAAGG C^C[AT][AT]GG TfiI 336 GATTC G^A[AT]TC Tth111I 775 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 727 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 783 CCAGTCGAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 485 CCCGGG C^CCGGG XmaI 485 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 658 GTCTAC GT^[AC][GT]AC 991 GTAGAC AccII 287 CGCG CG^CG 868 CGCG AvaII 510 GGTCC G^G[AT]CC 797 GGTCC BalI 735 TGGCCA TGG^CCA 1040 TGGCCA BanII 7 GAGCCC G[GA]GC[CT]^C 490 GAGCCC BbsI 169 GAAGAC GAAGAC(2/6) 534 GTCTTC BpuAI 169 GAAGAC GAAGAC(2/6) 534 GTCTTC BsrBI 356 CCGCTC CCGCTC(-3/-3) 450 CCGCTC BstUI 287 CGCG CG^CG 868 CGCG BstXI 494 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 816 CCACGTCCCTGG EaeI 735 TGGCCA [CT]^GGCC[GA] 1040 TGGCCA EcoNI 675 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 823 CCTGGCGGAGG MluNI 735 TGGCCA TGG^CCA 1040 TGGCCA MscI 735 TGGCCA TGG^CCA 1040 TGGCCA MspA1I 286 CCGCGG C[AC]G^C[GT]G 408 CAGCGG MvnI 287 CGCG CG^CG 868 CGCG PleI 994 GACTC GAGTC(4/5) 779 GAGTC SfaNI 951 GCATC GCATC(5/9) 980 GCATC SfcI 414 CTACAG C^T[GA][CT]AG 553 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp45I TspRI TthHB8I