(Circular) (Six-base) MAPSORT of: pFN21AB5788.seq Check: 6577 from: 1 to: 5761 pFN21AB5788 5761 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2664 2997 3104 1937 Size: 727 333 107 4594 Fragments arranged by size: 4594 727 333 107 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2160 2609 2648 3824 5208 5476 1632 Size: 528 449 39 1176 1384 268 1917 Fragments arranged by size: 1917 1384 1176 528 449 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2943 4660 5637 1203 Size: 1740 1717 977 1327 Fragments arranged by size: 1740 1717 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 3031 4490 3031 Size: 1459 4302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 3504 5076 3504 Size: 1572 4189 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2490 3086 1870 Size: 73 547 596 4545 Fragments arranged by size: 4545 596 547 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 4537 618 Size: 325 217 627 1295 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2499 3080 1391 Size: 625 483 581 4072 Fragments arranged by size: 4072 625 581 483 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2532 928 Size: 971 282 351 4157 Fragments arranged by size: 4157 971 351 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3017 3051 3952 3986 1903 Size: 34 1080 34 901 34 3678 Fragments arranged by size: 3678 1080 901 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 2319 3044 Size: 107 122 71 631 605 589 58 725 Cuts at: 3044 4372 136 Size: 1328 1525 Fragments arranged by size: 1525 1328 725 631 605 589 122 107 71 58 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2010 3178 2010 Size: 1168 4593 Bme1580I G_kGCm'C Cuts at: 1790 2577 3822 4978 1790 Size: 787 1245 1156 2573 Fragments arranged by size: 2573 1245 1156 787 BmgBI CAC'GTC Cuts at: 1318 2824 2946 1318 Size: 1506 122 4133 Fragments arranged by size: 4133 1506 122 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3023 1056 Size: 1967 3794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 4421 1239 Size: 1053 2129 2579 Fragments arranged by size: 2579 2129 1053 Bpu10I CC'TnA_GC Cuts at: 2681 2681 Size: 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2194 3552 493 Size: 711 990 1358 2702 Fragments arranged by size: 2702 1358 990 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 4009 Fragments arranged by size: 4009 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2962 1186 Size: 1776 3985 BseYI C'CCAG_C Cuts at: 2048 2165 2378 3006 4964 5460 2048 Size: 117 213 628 1958 496 2349 Fragments arranged by size: 2349 1958 628 496 213 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsiWI C'GTAC_G Cuts at: 2055 2055 Size: 5761 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 BsmBI CGTCTCn'nnnn_ Cuts at: 2952 2952 Size: 5761 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2499 2577 3080 3822 3907 4978 Size: 399 226 483 78 503 742 85 1071 Cuts at: 4978 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 503 483 399 226 85 78 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2363 2457 3648 1394 Size: 429 540 94 1191 3507 Fragments arranged by size: 3507 1191 540 429 94 BsrDI GCAATG_nn' Cuts at: 65 1248 2971 4256 4430 65 Size: 1183 1723 1285 174 1396 Fragments arranged by size: 1723 1396 1285 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2978 4405 4592 1700 Size: 110 1168 1427 187 2869 Fragments arranged by size: 2869 1427 1168 187 110 BsrGI T'GTAC_A Cuts at: 96 2916 96 Size: 2820 2941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 2028 4565 2028 Size: 2537 3224 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 4256 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2828 2506 Size: 322 5439 BstYI r'GATC_y Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756 Size: 1119 367 385 17 1452 86 12 346 Cuts at: 5756 1972 Size: 1977 Fragments arranged by size: 1977 1452 1119 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2291 2899 2951 513 Size: 552 939 287 608 52 3323 Fragments arranged by size: 3323 939 608 552 287 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2256 487 Size: 683 56 1030 3992 Fragments arranged by size: 3992 1030 683 56 BtsI GCAGTG_nn' Cuts at: 2517 3277 4071 4091 2517 Size: 760 794 20 4187 Fragments arranged by size: 4187 794 760 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3045 3208 Size: 54 1425 50 275 18 910 305 163 Cuts at: 3208 4097 5726 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 305 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2533 2847 2921 4348 4722 4868 Size: 1064 138 944 314 74 1427 374 146 Cuts at: 4868 387 Size: 1280 Fragments arranged by size: 1427 1280 1064 944 374 314 146 138 74 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2292 2609 2648 3824 4421 5208 Size: 393 528 132 317 39 1176 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 1176 787 597 528 393 317 268 132 39 EcoNI CCTnn'n_nnAGG Cuts at: 2684 2832 2684 Size: 148 5613 EcoO109I rG'GnC_Cy Cuts at: 2802 2802 Size: 5761 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 2884 2788 Size: 96 5665 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2188 2220 3017 Size: 32 135 32 454 32 130 32 797 Cuts at: 3017 3049 4402 4434 4476 4508 1373 Size: 32 1353 32 42 32 2626 Fragments arranged by size: 2626 1353 797 454 135 130 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3105 3351 669 Size: 988 281 739 428 246 3079 Fragments arranged by size: 3079 988 739 428 281 246 HindIII A'AGCT_T Cuts at: 748 2765 748 Size: 2017 3744 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2501 4875 5059 1305 Size: 1196 2374 184 2007 Fragments arranged by size: 2374 2007 1196 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2742 3047 10 Size: 54 1425 343 910 305 2724 Fragments arranged by size: 2724 1425 910 343 305 54 MslI CAynn'nnrTG Cuts at: 518 1166 2990 3719 4078 4237 518 Size: 648 1824 729 359 159 2042 Fragments arranged by size: 2042 1824 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2293 2415 3177 3854 5002 5247 1606 Size: 687 122 762 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 687 677 245 122 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 3019 1052 Size: 1967 3794 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 2738 1108 Size: 1630 4131 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2194 2194 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2802 2802 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2562 3113 830 Size: 1732 551 3478 Fragments arranged by size: 3478 1732 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 SacII CC_GC'GG Cuts at: 2294 2294 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SanDI GG'GwC_CC Cuts at: 2802 2802 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2419 2558 3109 4244 4925 5116 826 Size: 219 1374 139 551 1135 681 191 1471 Fragments arranged by size: 1471 1374 1135 681 551 219 191 139 SfiI GGCCn_nnn'nGGCC Cuts at: 2319 2319 Size: 5761 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 2492 3088 1872 Size: 620 596 4545 Fragments arranged by size: 4545 620 596 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2256 3200 513 Size: 552 939 252 944 3074 Fragments arranged by size: 3074 944 939 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2392 2500 2542 2834 3788 3805 3958 Size: 1255 812 108 42 292 954 17 153 Cuts at: 3958 4143 4588 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 954 812 445 292 185 153 108 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2916 4007 5719 Size: 274 80 33 51 1443 939 1091 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1091 939 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 5721 5753 2326 Size: 32 3363 32 2334 Fragments arranged by size: 3363 2334 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2783 1316 Size: 1467 4294 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2795 3055 2795 Size: 260 5501 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2490 3086 1870 Size: 620 596 4545 Fragments arranged by size: 4545 620 596 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfoI SrfI SwaI