################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AcsI 309 GAATTT [GA]^AATT[CT] AfaI 101 GTAC GT^AC AlwNI 222 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 7 GGGCCC GGGCC^C ApoI 309 GAATTT [GA]^AATT[CT] AspHI 268 GAGCAC G[AT]GC[AT]^C BpmI 190 CTCCAG CTGGAG(16/14) BsaJI 288 CCATGG C^C[ACGT][ACGT]GG BsiHKAI 268 GAGCAC G[AT]GC[AT]^C BsmI 70 GCATTC GAATGC(1/-1) BsrDI 41 CATTGC GCAATG(2/0) Cac8I 94 GCCTGC GC[ACGT]^[ACGT]GC DsaI 288 CCATGG C^C[GA][CT]GG EcoT14I 288 CCATGG C^C[AT][AT]GG HaeIII 8 GGCC GG^CC HinfI 294 GACTC G^A[ACGT]TC MslI 150 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 288 CCATGG C^CATGG NlaIII 289 CATG CATG^ PleI 294 GACTC GAGTC(4/5) RsaI 101 GTAC GT^AC StyI 288 CCATGG C^C[AT][AT]GG Tsp509I 310 AATT ^AATT ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanII 7 GGGCCC G[GA]GC[CT]^C 51 GAGCCC BsmFI 179 GGGAC GGGAC(10/14) 292 GGGAC BstAPI 89 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 221 GCAGCATCTGC Cfr13I 7 GGGCC G^G[ACGT]CC 8 GGCCC DraII 6 GGGGCCC [GA]G^G[ACGT]CC[CT] 7 GGGCCCC EcoO109I 6 GGGGCCC [GA]G^G[ACGT]CC[CT] 7 GGGCCCC HphI 186 TCACC GGTGA(8/7) 207 TCACC MaeIII 185 GTCAC ^GT[ACGT]AC 255 GTCAC PstI 21 CTGCAG CTGCA^G 163 CTGCAG Sau96I 7 GGGCC G^G[ACGT]CC 8 GGCCC Tsp45I 185 GTCAC ^GT[GC]AC 255 GTCAC TspRI 90 CACTG CA[GC]TG[ACGT][ACGT]^ 259 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI BbvI BmyI Bsp1286I BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI ItaI MnlI MvaI MwoI NlaIV ScrFI SfaNI SfcI TseI