################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HgaI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XmnI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### AccII 1410 CGCG CG^CG AcyI 6 GGCGCC G[GA]^CG[CT]C AflIII 1154 ACATGT A^C[GA][CT]GT AhdI 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1294 GTGCAC G^TGCAC AlwI 37 GGATC GGATC(4/5) AocI 1099 CCTGAGG CC^T[ACGT]AGG Aor51HI 140 AGCGCT AGC^GCT ApaI 360 GGGCCC GGGCC^C ApaLI 1294 GTGCAC G^TGCAC AspEI 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1145 TGCGCA TGC^GCA BbeI 6 GGCGCC GGCGC^C BfaI 235 CTAG C^TAG BglI 1003 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 372 CACGTA [CT]AC^GT[GA] BsaHI 6 GGCGCC G[GA]^CG[CT]C BsgI 169 CTGCAC GTGCAG(16/14) BsiWI 374 CGTACG C^GTACG BspHI 626 TCATGA T^CATGA BspLU11I 1154 ACATGT A^CATGT BsrDI 741 CATTGC GCAATG(2/0) BstAPI 317 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1488 GGTGACC G^GT[ACGT]ACC BstPI 1488 GGTGACC G^GT[ACGT]ACC BstUI 1410 CGCG CG^CG BstXI 1163 CCATGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1099 CCTGAGG CC^T[ACGT]AGG DraI 964 TTTAAA TTT^AAA Eam1105I 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 140 AGCGCT AGC^GCT Eco81I 1099 CCTGAGG CC^T[ACGT]AGG EcoNI 900 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1488 GGTGACC G^GT[ACGT]ACC EcoT14I 1163 CCATGG C^C[AT][AT]GG FspI 1145 TGCGCA TGC^GCA Hin1I 6 GGCGCC G[GA]^CG[CT]C HincII 1453 GTCAAC GT[CT]^[GA]AC HindII 1453 GTCAAC GT[CT]^[GA]AC KasI 6 GGCGCC G^GCGCC KspI 1409 CCGCGG CCGC^GG MaeI 235 CTAG C^TAG MaeII 373 ACGT A^CGT MvnI 1410 CGCG CG^CG NaeI 49 GCCGGC GCC^GGC NarI 6 GGCGCC GG^CGCC NcoI 1163 CCATGG C^CATGG NgoMIV 49 GCCGGC G^CCGGC PaeR7I 1108 CTCGAG C^TCGAG PflFI 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 349 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1525 GAGTC GAGTC(4/5) PpuMI 521 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1491 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 571 CAGCTG CAG^CTG RcaI 626 TCATGA T^CATGA SacI 211 GAGCTC GAGCT^C SacII 1409 CCGCGG CCGC^GG SapI 987 GAAGAGC GCTCTTC(1/4) SfoI 6 GGCGCC GGC^GCC SmaI 1341 CCCGGG CCC^GGG StuI 888 AGGCCT AGG^CCT StyI 1163 CCATGG C^C[AT][AT]GG TfiI 1440 GATTC G^A[AT]TC Tth111I 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 349 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1108 CTCGAG C^TCGAG XmaCI 1341 CCCGGG C^CCGGG XmaI 1341 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 350 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 571 CAGCTGCTG BalI 272 TGGCCA TGG^CCA 1254 TGGCCA BglII 650 AGATCT A^GATCT 695 AGATCT BsmBI 874 GAGACG CGTCTC(1/5) 1083 GAGACG BsrBI 129 GAGCGG CCGCTC(-3/-3) 1426 GAGCGG BsrFI 49 GCCGGC [GA]^CCGG[CT] 479 ACCGGC BsrI 104 ACTGG ACTGG(1/-1) 1328 ACTGG BstYI 650 AGATCT [GA]^GATC[CT] 695 AGATCT Cfr10I 49 GCCGGC [GA]^CCGG[CT] 479 ACCGGC EaeI 272 TGGCCA [CT]^GGCC[GA] 1254 TGGCCA EarI 987 GAAGAG CTCTTC(1/4) 1246 GAAGAG FauI 1411 GCGGG CCCGC(4/6) 1428 GCGGG HinfI 1440 GATTC G^A[ACGT]TC 1525 GAGTC HphI 1488 GGTGA GGTGA(8/7) 1499 TCACC Ksp632I 987 GAAGAG CTCTTC(1/4) 1246 GAAGAG MflI 650 AGATCT [GA]^GATC[CT] 695 AGATCT MluNI 272 TGGCCA TGG^CCA 1254 TGGCCA MscI 272 TGGCCA TGG^CCA 1254 TGGCCA MseI 965 TTAA T^TAA 1319 TTAA MspA1I 571 CAGCTG C[AC]G^C[GT]G 1409 CCGCGG NspI 1154 ACATGT [GA]CATG^[CT] 1229 ACATGC SmlI 829 CTCAAG C^T[CT][GA]AG 1108 CTCGAG Tru9I 965 TTAA T^TAA 1319 TTAA XcmI 101 CCAACTGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 516 CCATGAGGACCCTGG XhoII 650 AGATCT [GA]^GATC[CT] 695 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I