(Circular) (Six-base) MAPSORT of: pFN21AE0966.seq Check: 2882 from: 1 to: 6241 pFN21AE0966 6241 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4051 278 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2689 3011 4304 5688 5956 1632 Size: 953 104 322 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 953 322 268 104 AfeI AGC'GCT Cuts at: 2147 2147 Size: 6241 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 3159 5140 6117 1203 Size: 1956 1981 977 1327 Fragments arranged by size: 1981 1956 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 2677 4970 2677 Size: 2293 3948 AleI CACnn'nnGTG Cuts at: 1166 2086 3008 3509 1166 Size: 920 922 501 3898 Fragments arranged by size: 3898 922 920 501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6241 AlwNI CAG_nnn'CTG Cuts at: 2360 2581 3984 5556 2360 Size: 221 1403 1572 3045 Fragments arranged by size: 3045 1572 1403 221 ApaI G_GGCC'C Cuts at: 2369 2369 Size: 6241 ApaLI G'TGCA_C Cuts at: 3299 4298 5454 3299 Size: 999 1156 4086 Fragments arranged by size: 4086 1156 999 ApoI r'AATT_y Cuts at: 1493 3550 3776 3928 6037 1493 Size: 2057 226 152 2109 1697 Fragments arranged by size: 2109 2057 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2595 3113 3346 3566 1870 Size: 73 652 518 233 220 4545 Fragments arranged by size: 4545 652 518 233 220 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2207 2925 3562 5017 Size: 325 217 627 224 196 718 637 1455 Cuts at: 5017 618 Size: 1842 Fragments arranged by size: 1842 1455 718 637 627 325 224 217 196 BanII G_rGCy'C Cuts at: 1391 2220 2369 2819 3560 1391 Size: 829 149 450 741 4072 Fragments arranged by size: 4072 829 741 450 149 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 6241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5270 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4432 4466 1903 Size: 34 2495 34 3678 Fragments arranged by size: 3678 2495 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3014 4852 Size: 107 122 71 631 605 337 1005 1838 Cuts at: 4852 136 Size: 1525 Fragments arranged by size: 1838 1525 1005 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2655 2700 6236 2655 Size: 45 3536 2660 Fragments arranged by size: 3536 2660 45 BlpI GC'TnA_GC Cuts at: 3658 3658 Size: 6241 Bme1580I G_kGCm'C Cuts at: 1790 2369 2922 3303 4302 5458 1790 Size: 579 553 381 999 1156 2573 Fragments arranged by size: 2573 1156 999 579 553 381 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6241 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2119 3972 4930 Size: 416 53 83 399 24 702 1853 958 Cuts at: 4930 442 Size: 1753 Fragments arranged by size: 1853 1753 958 702 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2162 2603 2651 3042 4901 1239 Size: 899 24 441 48 391 1859 2579 Fragments arranged by size: 2579 1859 899 441 391 48 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 4366 5231 5529 2819 Size: 1547 865 298 3531 Fragments arranged by size: 3531 1547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4904 882 Size: 617 3405 2219 Fragments arranged by size: 3405 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2379 4032 493 Size: 711 1175 1653 2702 Fragments arranged by size: 2702 1653 1175 711 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 4048 4430 5065 Size: 53 83 186 936 479 2036 382 635 Cuts at: 5065 275 Size: 1451 Fragments arranged by size: 2036 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4674 5072 5346 5493 5904 4674 Size: 398 274 147 411 5011 Fragments arranged by size: 5011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2801 2831 3179 3209 3422 Size: 30 165 30 1398 30 348 30 213 Cuts at: 3422 3452 1178 Size: 30 3967 Fragments arranged by size: 3967 1398 348 213 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2300 3293 3392 3443 1186 Size: 838 276 993 99 51 3984 Fragments arranged by size: 3984 993 838 276 99 51 BseYI C'CCAG_C Cuts at: 2360 2625 3357 3384 5444 5940 2360 Size: 265 732 27 2060 496 2661 Fragments arranged by size: 2661 2060 732 496 265 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 1163 Size: 573 423 5245 Fragments arranged by size: 5245 573 423 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 3114 3303 4302 4387 5458 2220 Size: 894 189 999 85 1071 3003 Fragments arranged by size: 3003 1071 999 894 189 85 BsiWI C'GTAC_G Cuts at: 2379 2379 Size: 6241 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 BsmBI CGTCTCn'nnnn_ Cuts at: 2873 3082 2873 Size: 209 6032 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2369 2819 2922 3114 3303 3560 Size: 399 430 149 450 103 192 189 257 Cuts at: 3560 4302 4387 5458 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 430 399 257 192 189 149 103 85 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 2631 4130 5860 2631 Size: 1499 1730 3012 Fragments arranged by size: 3012 1730 1499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2742 2913 3525 3596 844 Size: 1898 171 612 71 3489 Fragments arranged by size: 3489 1898 612 171 71 BsrBI CCG'CTC Cuts at: 1394 1823 2136 3433 4128 1394 Size: 429 313 1297 695 3507 Fragments arranged by size: 3507 1297 695 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 2745 4736 4910 65 Size: 1183 1497 1991 174 1396 Fragments arranged by size: 1991 1497 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 2484 4885 5072 1700 Size: 110 244 430 2401 187 2869 Fragments arranged by size: 2869 2401 430 244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 2219 2726 2889 3085 4301 5313 2219 Size: 507 163 196 1216 1012 3147 Fragments arranged by size: 3147 1216 1012 507 196 163 BstAPI GCAn_nnn'nTGC Cuts at: 794 2328 794 Size: 1534 4707 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 3493 1184 Size: 2309 3932 BstXI CCAn_nnnn'nTGG Cuts at: 3175 3175 Size: 6241 BstYI r'GATC_y Cuts at: 1972 2655 2700 3571 3938 4323 4340 5792 5878 Size: 683 45 871 367 385 17 1452 86 Cuts at: 5878 5890 6236 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 871 683 385 367 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 3105 3105 Size: 6241 BtgI C'CryG_G Cuts at: 513 1065 2929 3070 3168 3222 3414 513 Size: 552 1864 141 98 54 192 3340 Fragments arranged by size: 3340 1864 552 192 141 98 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2474 487 Size: 683 56 1248 4254 Fragments arranged by size: 4254 1248 683 56 BtsI GCAGTG_nn' Cuts at: 2176 2429 2570 3757 4551 4571 2176 Size: 253 141 1187 794 20 3846 Fragments arranged by size: 3846 1187 794 253 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 2971 3545 2971 Size: 574 5667 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2277 3259 3688 Size: 54 1425 50 275 18 447 982 429 Cuts at: 3688 4577 6206 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 982 889 447 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2987 3246 4171 1353 Size: 1634 259 925 3423 Fragments arranged by size: 3423 1634 925 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 2218 Size: 6241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2162 2585 2603 2651 2689 3011 Size: 393 506 24 423 18 48 38 322 Cuts at: 3011 3042 4304 4901 5688 5956 1239 Size: 31 1262 597 787 268 1524 Fragments arranged by size: 1524 1262 787 597 506 423 393 322 268 48 38 31 24 18 EcoNI CCTnn'n_nnAGG Cuts at: 2909 2909 Size: 6241 EcoO109I rG'GnC_Cy Cuts at: 2365 2446 2527 2365 Size: 81 81 6079 Fragments arranged by size: 6079 81 81 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FspI TGC'GCA Cuts at: 3152 4747 3152 Size: 1595 4646 HaeII r_GCGC'y Cuts at: 1678 2015 2149 2605 5388 1678 Size: 337 134 456 2783 2531 Fragments arranged by size: 2783 2531 456 337 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 2663 2695 2801 Size: 32 135 32 1006 32 53 32 106 Cuts at: 2801 2833 2870 2902 3179 3211 3422 3454 4882 Size: 32 37 32 277 32 211 32 1428 Cuts at: 4882 4914 4956 4988 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1428 1006 277 211 135 106 53 42 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3460 3585 3831 669 Size: 988 281 1522 125 246 3079 Fragments arranged by size: 3079 1522 988 281 246 125 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KasI G'GCGC_C Cuts at: 2011 2011 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2793 3255 5355 5539 1305 Size: 1488 462 2100 184 2007 Fragments arranged by size: 2100 2007 1488 462 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 3261 10 Size: 54 1425 343 447 982 2990 Fragments arranged by size: 2990 1425 982 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2086 2913 3008 3158 3509 4199 4558 Size: 648 920 827 95 150 351 690 359 Cuts at: 4558 4717 518 Size: 159 2042 Fragments arranged by size: 2042 920 827 690 648 359 351 159 150 95 MspA1I CmG'CkG Cuts at: 1606 2578 3416 3657 4334 5482 5727 1606 Size: 972 838 241 677 1148 245 2120 Fragments arranged by size: 2120 1148 972 838 677 245 241 NaeI GCC'GGC Cuts at: 2056 2056 Size: 6241 NarI GG'CG_CC Cuts at: 2012 2012 Size: 6241 NcoI C'CATG_G Cuts at: 513 1065 3168 513 Size: 552 2103 3586 Fragments arranged by size: 3586 2103 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 3163 3238 3599 5144 1132 Size: 97 1934 75 361 1545 2229 Fragments arranged by size: 2229 1934 1545 361 97 75 PasI CC'CwG_GG Cuts at: 1348 2016 2531 1348 Size: 668 515 5058 Fragments arranged by size: 5058 668 515 PciI A'CATG_T Cuts at: 3159 5140 3159 Size: 1981 4260 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 1108 Size: 1252 4989 PfoI T'CCnGG_A Cuts at: 2667 2667 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2527 Size: 6241 PshAI GACnn'nnGTC Cuts at: 1613 3500 1613 Size: 1887 4354 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspOMI G'GGCC_C Cuts at: 2365 2365 Size: 6241 PspXI vC'TCGA_Gb Cuts at: 3113 3113 Size: 6241 PstI C_TGCA'G Cuts at: 830 2577 3214 3288 3593 830 Size: 1747 637 74 305 3478 Fragments arranged by size: 3478 1747 637 305 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2578 2578 Size: 6241 SacI G_AGCT'C Cuts at: 2220 2220 Size: 6241 SacII CC_GC'GG Cuts at: 3417 3417 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2987 2987 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 4489 6201 4489 Size: 1712 4529 SfcI C'TryA_G Cuts at: 826 1045 2573 2738 3210 3284 3589 4724 5405 Size: 219 1528 165 472 74 305 1135 681 Cuts at: 5405 5596 826 Size: 191 1471 Fragments arranged by size: 1528 1471 1135 681 472 305 219 191 165 74 SfoI GGC'GCC Cuts at: 2013 2013 Size: 6241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 3348 3568 1872 Size: 1476 220 4545 Fragments arranged by size: 4545 1476 220 SmlI C'TyrA_G Cuts at: 820 1017 1943 2834 3113 4345 5246 5508 820 Size: 197 926 891 279 1232 901 262 1553 Fragments arranged by size: 1553 1232 926 901 891 279 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 2895 1517 Size: 1378 4863 StyI C'CwwG_G Cuts at: 513 1065 3168 3680 513 Size: 552 2103 512 3074 Fragments arranged by size: 3074 2103 552 512 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 3083 3445 4268 4285 4438 4623 Size: 1255 428 1075 362 823 17 153 185 Cuts at: 4623 5068 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1075 823 445 428 362 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 3231 4487 Size: 274 80 33 51 1443 324 930 1256 Cuts at: 4487 6199 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1256 930 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6201 6233 6201 Size: 32 6209 Tth111I GACn'n_nGTC Cuts at: 1316 2884 1316 Size: 1568 4673 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2528 2113 Size: 415 5826 XhoI C'TCGA_G Cuts at: 1943 3113 1943 Size: 1170 5071 XmaI C'CCGG_G Cuts at: 1870 3346 3566 1870 Size: 1476 220 4545 Fragments arranged by size: 4545 1476 220 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4049 276 Size: 53 83 186 936 2515 2468 Fragments arranged by size: 2515 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SexAI SfiI SrfI SwaI