Restriction map for pFN21AE0966
 (Linear) (Six-base) MAPSORT of: KIEE0966.seq  Check: 3721  from: 1  to: 1536



KIEE0966.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:41  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     684    1006    1536 
   Size:        580     104     322     530

  Fragments arranged by size: 

                 580     530     322     104
 
AfeI AGC'GCT

Cuts at:      0     142    1536 
   Size:        142    1394
 
AflIII A'CryG_T

Cuts at:      0    1154    1536 
   Size:       1154     382
 
AhdI GACnn_n'nnGTC

Cuts at:      0     672    1536 
   Size:        672     864
 
AleI CACnn'nnGTG

Cuts at:      0      81    1003    1504    1536 
   Size:         81     922     501      32

  Fragments arranged by size: 

                 922     501      81      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355     576    1536 
   Size:        355     221     960

  Fragments arranged by size: 

                 960     355     221
 
ApaI G_GGCC'C

Cuts at:      0     364    1536 
   Size:        364    1172
 
ApaLI G'TGCA_C

Cuts at:      0    1294    1536 
   Size:       1294     242
 
AvaI C'yCGr_G

Cuts at:      0     590    1108    1341    1536 
   Size:        590     518     233     195

  Fragments arranged by size: 

                 590     518     233     195
 
BanI G'GyrC_C

Cuts at:      0       6     202     920    1536 
   Size:          6     196     718     616

  Fragments arranged by size: 

                 718     616     196       6
 
BanII G_rGCy'C

Cuts at:      0     215     364     814    1536 
   Size:        215     149     450     722

  Fragments arranged by size: 

                 722     450     215     149
 
BbeI G_GCGC'C

Cuts at:      0      10    1536 
   Size:         10    1526
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1009    1536 
   Size:       1009     527
 
BglII A'GATC_T

Cuts at:      0     650     695    1536 
   Size:        650      45     841

  Fragments arranged by size: 

                 841     650      45
 
Bme1580I G_kGCm'C

Cuts at:      0     364     917    1298    1536 
   Size:        364     553     381     238

  Fragments arranged by size: 

                 553     381     364     238
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     114    1536 
   Size:        114    1422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     157     598     646    1037    1536 
   Size:        133      24     441      48     391     499

  Fragments arranged by size: 

                 499     441     391     133      48      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     814    1536 
   Size:        814     722
 
BsaAI yAC'GTr

Cuts at:      0     374    1536 
   Size:        374    1162
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1536 
   Size:          7    1529
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     796     826    1174    1204    1417    1447    1536 
   Size:        796      30     348      30     213      30      89

  Fragments arranged by size: 

                 796     348     213      89      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     295    1288    1387    1438    1536 
   Size:         19     276     993      99      51      98

  Fragments arranged by size: 

                 993     276      99      98      51      19
 
BseYI C'CCAG_C

Cuts at:      0     355     620    1352    1379    1536 
   Size:        355     265     732      27     157

  Fragments arranged by size: 

                 732     355     265     157      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1536 
   Size:        154    1382
 
BsiHKAI G_wGCw'C

Cuts at:      0     215    1109    1298    1536 
   Size:        215     894     189     238

  Fragments arranged by size: 

                 894     238     215     189
 
BsiWI C'GTAC_G

Cuts at:      0     374    1536 
   Size:        374    1162
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     868    1077    1536 
   Size:        868     209     459

  Fragments arranged by size: 

                 868     459     209
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     364     814     917    1109    1298    1536 
   Size:        215     149     450     103     192     189     238

  Fragments arranged by size: 

                 450     238     215     192     189     149     103
 
BspHI T'CATG_A

Cuts at:      0     626    1536 
   Size:        626     910
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     737     908    1520    1536 
   Size:        737     171     612      16

  Fragments arranged by size: 

                 737     612     171      16
 
BsrBI CCG'CTC

Cuts at:      0     131    1428    1536 
   Size:        131    1297     108

  Fragments arranged by size: 

                1297     131     108
 
BsrDI GCAATG_nn'

Cuts at:      0     740    1536 
   Size:        740     796
 
BsrFI r'CCGG_y

Cuts at:      0      49     479    1536 
   Size:         49     430    1057

  Fragments arranged by size: 

                1057     430      49
 
BssSI C'ACGA_G

Cuts at:      0     214     721     884    1080    1536 
   Size:        214     507     163     196     456

  Fragments arranged by size: 

                 507     456     214     196     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     323    1536 
   Size:        323    1213
 
BstEII G'GTnAC_C

Cuts at:      0    1488    1536 
   Size:       1488      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1170    1536 
   Size:       1170     366
 
BstYI r'GATC_y

Cuts at:      0     650     695    1536 
   Size:        650      45     841

  Fragments arranged by size: 

                 841     650      45
 
Bsu36I CC'TnA_GG

Cuts at:      0    1100    1536 
   Size:       1100     436
 
BtgI C'CryG_G

Cuts at:      0     924    1065    1163    1217    1409    1536 
   Size:        924     141      98      54     192     127

  Fragments arranged by size: 

                 924     192     141     127      98      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     469    1536 
   Size:        469    1067
 
BtsI GCAGTG_nn'

Cuts at:      0     171     424     565    1536 
   Size:        171     253     141     971

  Fragments arranged by size: 

                 971     253     171     141
 
DraI TTT'AAA

Cuts at:      0     966    1536 
   Size:        966     570
 
EaeI y'GGCC_r

Cuts at:      0     272    1254    1536 
   Size:        272     982     282

  Fragments arranged by size: 

                 982     282     272
 
EarI CTCTTCn'nnn_

Cuts at:      0     982    1241    1536 
   Size:        982     259     295

  Fragments arranged by size: 

                 982     295     259
 
EcoICRI GAG'CTC

Cuts at:      0     213    1536 
   Size:        213    1323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     157     580     598     646     684    1006    1037 
   Size:        133      24     423      18      48      38     322      31

Cuts at:   1037    1536 
   Size:        499

  Fragments arranged by size: 

                 499     423     322     133      48      38      31      24
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     904    1536 
   Size:        904     632
 
EcoO109I rG'GnC_Cy

Cuts at:      0     360     441     522    1536 
   Size:        360      81      81    1014

  Fragments arranged by size: 

                1014     360      81      81
 
FspI TGC'GCA

Cuts at:      0    1147    1536 
   Size:       1147     389
 
HaeII r_GCGC'y

Cuts at:      0      10     144     600    1536 
   Size:         10     134     456     936

  Fragments arranged by size: 

                 936     456     134      10
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     573     605     658     690     796     828     865     897 
   Size:        573      32      53      32     106      32      37      32

Cuts at:    897    1174    1206    1417    1449    1536 
   Size:        277      32     211      32      87

  Fragments arranged by size: 

                 573     277     211     106      87      53      37      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1455    1536 
   Size:       1455      81
 
KasI G'GCGC_C

Cuts at:      0       6    1536 
   Size:          6    1530
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     788    1250    1536 
   Size:        788     462     286

  Fragments arranged by size: 

                 788     462     286
 
MscI TGG'CCA

Cuts at:      0     274    1256    1536 
   Size:        274     982     280

  Fragments arranged by size: 

                 982     280     274
 
MslI CAynn'nnrTG

Cuts at:      0      81     908    1003    1153    1504    1536 
   Size:         81     827      95     150     351      32

  Fragments arranged by size: 

                 827     351     150      95      81      32
 
MspA1I CmG'CkG

Cuts at:      0     573    1411    1536 
   Size:        573     838     125

  Fragments arranged by size: 

                 838     573     125
 
NaeI GCC'GGC

Cuts at:      0      51    1536 
   Size:         51    1485
 
NarI GG'CG_CC

Cuts at:      0       7    1536 
   Size:          7    1529
 
NcoI C'CATG_G

Cuts at:      0    1163    1536 
   Size:       1163     373
 
NgoMIV G'CCGG_C

Cuts at:      0      49    1536 
   Size:         49    1487
 
NspI r_CATG'y

Cuts at:      0    1158    1233    1536 
   Size:       1158      75     303

  Fragments arranged by size: 

                1158     303      75
 
PasI CC'CwG_GG

Cuts at:      0      11     526    1536 
   Size:         11     515    1010

  Fragments arranged by size: 

                1010     515      11
 
PciI A'CATG_T

Cuts at:      0    1154    1536 
   Size:       1154     382
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     355    1536 
   Size:        355    1181
 
PfoI T'CCnGG_A

Cuts at:      0     662    1536 
   Size:        662     874
 
PpuMI rG'GwC_Cy

Cuts at:      0     522    1536 
   Size:        522    1014
 
PshAI GACnn'nnGTC

Cuts at:      0    1495    1536 
   Size:       1495      41
 
PspOMI G'GGCC_C

Cuts at:      0     360    1536 
   Size:        360    1176
 
PspXI vC'TCGA_Gb

Cuts at:      0    1108    1536 
   Size:       1108     428
 
PstI C_TGCA'G

Cuts at:      0     572    1209    1283    1536 
   Size:        572     637      74     253

  Fragments arranged by size: 

                 637     572     253      74
 
PvuII CAG'CTG

Cuts at:      0     573    1536 
   Size:        573     963
 
SacI G_AGCT'C

Cuts at:      0     215    1536 
   Size:        215    1321
 
SacII CC_GC'GG

Cuts at:      0    1412    1536 
   Size:       1412     124
 
SapI GCTCTTCn'nnn_

Cuts at:      0     982    1536 
   Size:        982     554
 
SfcI C'TryA_G

Cuts at:      0     568     733    1205    1279    1536 
   Size:        568     165     472      74     257

  Fragments arranged by size: 

                 568     472     257     165      74
 
SfoI GGC'GCC

Cuts at:      0       8    1536 
   Size:          8    1528
 
SmaI CCC'GGG

Cuts at:      0    1343    1536 
   Size:       1343     193
 
SmlI C'TyrA_G

Cuts at:      0     829    1108    1536 
   Size:        829     279     428

  Fragments arranged by size: 

                 829     428     279
 
StuI AGG'CCT

Cuts at:      0     890    1536 
   Size:        890     646
 
StyI C'CwwG_G

Cuts at:      0    1163    1536 
   Size:       1163     373
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3    1078    1440    1536 
   Size:          3    1075     362      96

  Fragments arranged by size: 

                1075     362      96       3
 
TatI w'GTAC_w

Cuts at:      0     296    1226    1536 
   Size:        296     930     310

  Fragments arranged by size: 

                 930     310     296
 
Tth111I GACn'n_nGTC

Cuts at:      0     879    1536 
   Size:        879     657
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     523    1536 
   Size:        108     415    1013

  Fragments arranged by size: 

                1013     415     108
 
XhoI C'TCGA_G

Cuts at:      0    1108    1536 
   Size:       1108     428
 
XmaI C'CCGG_G

Cuts at:      0    1341    1536 
   Size:       1341     195
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BglI    BglII Bme1580I     BmrI
     BpmI    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     SapI     SfcI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BsiEI     BsmI
    BspEI    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI   DraIII
     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI     PsiI     PsrI
     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com