(Linear) (Six-base) MAPSORT of: KIEE0966.seq Check: 3721 from: 1 to: 1536 KIEE0966.orf With 182 enzymes: SgfI * July 3, 2012 16:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 684 1006 1536 Size: 580 104 322 530 Fragments arranged by size: 580 530 322 104 AfeI AGC'GCT Cuts at: 0 142 1536 Size: 142 1394 AflIII A'CryG_T Cuts at: 0 1154 1536 Size: 1154 382 AhdI GACnn_n'nnGTC Cuts at: 0 672 1536 Size: 672 864 AleI CACnn'nnGTG Cuts at: 0 81 1003 1504 1536 Size: 81 922 501 32 Fragments arranged by size: 922 501 81 32 AlwNI CAG_nnn'CTG Cuts at: 0 355 576 1536 Size: 355 221 960 Fragments arranged by size: 960 355 221 ApaI G_GGCC'C Cuts at: 0 364 1536 Size: 364 1172 ApaLI G'TGCA_C Cuts at: 0 1294 1536 Size: 1294 242 AvaI C'yCGr_G Cuts at: 0 590 1108 1341 1536 Size: 590 518 233 195 Fragments arranged by size: 590 518 233 195 BanI G'GyrC_C Cuts at: 0 6 202 920 1536 Size: 6 196 718 616 Fragments arranged by size: 718 616 196 6 BanII G_rGCy'C Cuts at: 0 215 364 814 1536 Size: 215 149 450 722 Fragments arranged by size: 722 450 215 149 BbeI G_GCGC'C Cuts at: 0 10 1536 Size: 10 1526 BglI GCCn_nnn'nGGC Cuts at: 0 1009 1536 Size: 1009 527 BglII A'GATC_T Cuts at: 0 650 695 1536 Size: 650 45 841 Fragments arranged by size: 841 650 45 Bme1580I G_kGCm'C Cuts at: 0 364 917 1298 1536 Size: 364 553 381 238 Fragments arranged by size: 553 381 364 238 BmrI ACTGGGnnnn_n' Cuts at: 0 114 1536 Size: 114 1422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 157 598 646 1037 1536 Size: 133 24 441 48 391 499 Fragments arranged by size: 499 441 391 133 48 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 1536 Size: 814 722 BsaAI yAC'GTr Cuts at: 0 374 1536 Size: 374 1162 BsaHI Gr'CG_yC Cuts at: 0 7 1536 Size: 7 1529 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 796 826 1174 1204 1417 1447 1536 Size: 796 30 348 30 213 30 89 Fragments arranged by size: 796 348 213 89 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 295 1288 1387 1438 1536 Size: 19 276 993 99 51 98 Fragments arranged by size: 993 276 99 98 51 19 BseYI C'CCAG_C Cuts at: 0 355 620 1352 1379 1536 Size: 355 265 732 27 157 Fragments arranged by size: 732 355 265 157 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 1536 Size: 154 1382 BsiHKAI G_wGCw'C Cuts at: 0 215 1109 1298 1536 Size: 215 894 189 238 Fragments arranged by size: 894 238 215 189 BsiWI C'GTAC_G Cuts at: 0 374 1536 Size: 374 1162 BsmBI CGTCTCn'nnnn_ Cuts at: 0 868 1077 1536 Size: 868 209 459 Fragments arranged by size: 868 459 209 Bsp1286I G_dGCh'C Cuts at: 0 215 364 814 917 1109 1298 1536 Size: 215 149 450 103 192 189 238 Fragments arranged by size: 450 238 215 192 189 149 103 BspHI T'CATG_A Cuts at: 0 626 1536 Size: 626 910 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 737 908 1520 1536 Size: 737 171 612 16 Fragments arranged by size: 737 612 171 16 BsrBI CCG'CTC Cuts at: 0 131 1428 1536 Size: 131 1297 108 Fragments arranged by size: 1297 131 108 BsrDI GCAATG_nn' Cuts at: 0 740 1536 Size: 740 796 BsrFI r'CCGG_y Cuts at: 0 49 479 1536 Size: 49 430 1057 Fragments arranged by size: 1057 430 49 BssSI C'ACGA_G Cuts at: 0 214 721 884 1080 1536 Size: 214 507 163 196 456 Fragments arranged by size: 507 456 214 196 163 BstAPI GCAn_nnn'nTGC Cuts at: 0 323 1536 Size: 323 1213 BstEII G'GTnAC_C Cuts at: 0 1488 1536 Size: 1488 48 BstXI CCAn_nnnn'nTGG Cuts at: 0 1170 1536 Size: 1170 366 BstYI r'GATC_y Cuts at: 0 650 695 1536 Size: 650 45 841 Fragments arranged by size: 841 650 45 Bsu36I CC'TnA_GG Cuts at: 0 1100 1536 Size: 1100 436 BtgI C'CryG_G Cuts at: 0 924 1065 1163 1217 1409 1536 Size: 924 141 98 54 192 127 Fragments arranged by size: 924 192 141 127 98 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 469 1536 Size: 469 1067 BtsI GCAGTG_nn' Cuts at: 0 171 424 565 1536 Size: 171 253 141 971 Fragments arranged by size: 971 253 171 141 DraI TTT'AAA Cuts at: 0 966 1536 Size: 966 570 EaeI y'GGCC_r Cuts at: 0 272 1254 1536 Size: 272 982 282 Fragments arranged by size: 982 282 272 EarI CTCTTCn'nnn_ Cuts at: 0 982 1241 1536 Size: 982 259 295 Fragments arranged by size: 982 295 259 EcoICRI GAG'CTC Cuts at: 0 213 1536 Size: 213 1323 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 157 580 598 646 684 1006 1037 Size: 133 24 423 18 48 38 322 31 Cuts at: 1037 1536 Size: 499 Fragments arranged by size: 499 423 322 133 48 38 31 24 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 904 1536 Size: 904 632 EcoO109I rG'GnC_Cy Cuts at: 0 360 441 522 1536 Size: 360 81 81 1014 Fragments arranged by size: 1014 360 81 81 FspI TGC'GCA Cuts at: 0 1147 1536 Size: 1147 389 HaeII r_GCGC'y Cuts at: 0 10 144 600 1536 Size: 10 134 456 936 Fragments arranged by size: 936 456 134 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 573 605 658 690 796 828 865 897 Size: 573 32 53 32 106 32 37 32 Cuts at: 897 1174 1206 1417 1449 1536 Size: 277 32 211 32 87 Fragments arranged by size: 573 277 211 106 87 53 37 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1455 1536 Size: 1455 81 KasI G'GCGC_C Cuts at: 0 6 1536 Size: 6 1530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 788 1250 1536 Size: 788 462 286 Fragments arranged by size: 788 462 286 MscI TGG'CCA Cuts at: 0 274 1256 1536 Size: 274 982 280 Fragments arranged by size: 982 280 274 MslI CAynn'nnrTG Cuts at: 0 81 908 1003 1153 1504 1536 Size: 81 827 95 150 351 32 Fragments arranged by size: 827 351 150 95 81 32 MspA1I CmG'CkG Cuts at: 0 573 1411 1536 Size: 573 838 125 Fragments arranged by size: 838 573 125 NaeI GCC'GGC Cuts at: 0 51 1536 Size: 51 1485 NarI GG'CG_CC Cuts at: 0 7 1536 Size: 7 1529 NcoI C'CATG_G Cuts at: 0 1163 1536 Size: 1163 373 NgoMIV G'CCGG_C Cuts at: 0 49 1536 Size: 49 1487 NspI r_CATG'y Cuts at: 0 1158 1233 1536 Size: 1158 75 303 Fragments arranged by size: 1158 303 75 PasI CC'CwG_GG Cuts at: 0 11 526 1536 Size: 11 515 1010 Fragments arranged by size: 1010 515 11 PciI A'CATG_T Cuts at: 0 1154 1536 Size: 1154 382 PflMI CCAn_nnn'nTGG Cuts at: 0 355 1536 Size: 355 1181 PfoI T'CCnGG_A Cuts at: 0 662 1536 Size: 662 874 PpuMI rG'GwC_Cy Cuts at: 0 522 1536 Size: 522 1014 PshAI GACnn'nnGTC Cuts at: 0 1495 1536 Size: 1495 41 PspOMI G'GGCC_C Cuts at: 0 360 1536 Size: 360 1176 PspXI vC'TCGA_Gb Cuts at: 0 1108 1536 Size: 1108 428 PstI C_TGCA'G Cuts at: 0 572 1209 1283 1536 Size: 572 637 74 253 Fragments arranged by size: 637 572 253 74 PvuII CAG'CTG Cuts at: 0 573 1536 Size: 573 963 SacI G_AGCT'C Cuts at: 0 215 1536 Size: 215 1321 SacII CC_GC'GG Cuts at: 0 1412 1536 Size: 1412 124 SapI GCTCTTCn'nnn_ Cuts at: 0 982 1536 Size: 982 554 SfcI C'TryA_G Cuts at: 0 568 733 1205 1279 1536 Size: 568 165 472 74 257 Fragments arranged by size: 568 472 257 165 74 SfoI GGC'GCC Cuts at: 0 8 1536 Size: 8 1528 SmaI CCC'GGG Cuts at: 0 1343 1536 Size: 1343 193 SmlI C'TyrA_G Cuts at: 0 829 1108 1536 Size: 829 279 428 Fragments arranged by size: 829 428 279 StuI AGG'CCT Cuts at: 0 890 1536 Size: 890 646 StyI C'CwwG_G Cuts at: 0 1163 1536 Size: 1163 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3 1078 1440 1536 Size: 3 1075 362 96 Fragments arranged by size: 1075 362 96 3 TatI w'GTAC_w Cuts at: 0 296 1226 1536 Size: 296 930 310 Fragments arranged by size: 930 310 296 Tth111I GACn'n_nGTC Cuts at: 0 879 1536 Size: 879 657 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 108 523 1536 Size: 108 415 1013 Fragments arranged by size: 1013 415 108 XhoI C'TCGA_G Cuts at: 0 1108 1536 Size: 1108 428 XmaI C'CCGG_G Cuts at: 0 1341 1536 Size: 1341 195 Enzymes that do cut: AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BglI BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SapI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BsiEI BsmI BspEI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XmnI ZraI