(Circular) (Six-base) MAPSORT of: pFN21AB9642.seq Check: 6450 from: 1 to: 6484 pFN21AB9642 6484 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3239 3239 Size: 6484 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2996 3212 4547 5931 6199 1632 Size: 1364 216 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1364 1335 268 216 AfeI AGC'GCT Cuts at: 2570 2570 Size: 6484 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 3641 1166 Size: 2475 4009 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AlwNI CAG_nnn'CTG Cuts at: 2485 2591 4227 5799 2485 Size: 106 1636 1572 3170 Fragments arranged by size: 3170 1636 1572 106 ApaI G_GGCC'C Cuts at: 2674 2674 Size: 6484 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 3793 4019 4171 6280 1493 Size: 2300 226 152 2109 1697 Fragments arranged by size: 2300 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3809 1870 Size: 73 1866 4545 Fragments arranged by size: 4545 1866 73 BamHI G'GATC_C Cuts at: 3564 3814 4181 3564 Size: 250 367 5867 Fragments arranged by size: 5867 367 250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 3724 3805 5260 618 Size: 325 217 627 1016 921 81 1455 1842 Fragments arranged by size: 1842 1455 1016 921 627 325 217 81 BanII G_rGCy'C Cuts at: 1391 2196 2229 2674 2760 2801 3803 1391 Size: 805 33 445 86 41 1002 4072 Fragments arranged by size: 4072 1002 805 445 86 41 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2377 928 Size: 971 478 5035 Fragments arranged by size: 5035 971 478 BbvCI CC'TCA_GC Cuts at: 2276 2579 2828 2276 Size: 303 249 5932 Fragments arranged by size: 5932 303 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2339 2373 4675 4709 1903 Size: 34 402 34 2302 34 3678 Fragments arranged by size: 3678 2302 402 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 3220 5095 Size: 107 122 71 631 605 372 1176 1875 Cuts at: 5095 136 Size: 1525 Fragments arranged by size: 1875 1525 1176 631 605 372 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 2891 3901 2891 Size: 1010 5474 Bme1580I G_kGCm'C Cuts at: 1790 2674 2808 3225 4545 5701 1790 Size: 884 134 417 1320 1156 2573 Fragments arranged by size: 2573 1320 1156 884 417 134 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4215 5173 442 Size: 416 53 83 399 24 2798 958 1753 Fragments arranged by size: 2798 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2543 1056 Size: 1487 4997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3436 3436 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2097 2177 2190 2213 2246 2462 2507 2585 Size: 858 80 13 23 33 216 45 78 Cuts at: 2585 2723 2747 2894 3027 3201 3333 3431 5144 Size: 138 24 147 133 174 132 98 1713 Cuts at: 5144 1239 Size: 2579 Fragments arranged by size: 2579 1713 858 216 174 147 138 133 132 98 80 78 45 33 24 23 13 Bpu10I CC'TnA_GC Cuts at: 2252 2276 2579 2828 2252 Size: 24 303 249 5908 Fragments arranged by size: 5908 303 249 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2321 2678 3082 4609 5474 5772 2321 Size: 357 404 1527 865 298 3033 Fragments arranged by size: 3033 1527 865 404 357 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3315 5147 882 Size: 617 1816 1832 2219 Fragments arranged by size: 2219 1832 1816 617 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4291 4673 5308 275 Size: 53 83 186 936 2758 382 635 1451 Fragments arranged by size: 2758 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 1178 Size: 30 165 30 699 30 5530 Fragments arranged by size: 5530 699 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2057 3092 3296 1186 Size: 868 3 1035 204 4374 Fragments arranged by size: 4374 1035 868 204 3 BseYI C'CCAG_C Cuts at: 2121 2666 2683 2878 3325 3367 3377 3473 5687 Size: 545 17 195 447 42 10 96 2214 Cuts at: 5687 6183 2121 Size: 496 2422 Fragments arranged by size: 2422 2214 545 496 447 195 96 42 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2098 3446 1163 Size: 573 362 1348 4201 Fragments arranged by size: 4201 1348 573 362 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2196 2555 2801 4545 4630 5701 2139 Size: 57 359 246 1744 85 1071 2922 Fragments arranged by size: 2922 1744 1071 359 246 85 57 BsmI GAATG_Cn' Cuts at: 2244 3995 4088 2244 Size: 1751 93 4640 Fragments arranged by size: 4640 1751 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2615 2615 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2196 2229 2555 2674 2760 2801 Size: 399 349 57 33 326 119 86 41 Cuts at: 2801 2808 3225 3803 4545 4630 5701 1391 Size: 7 417 578 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 417 399 349 326 119 86 85 57 41 33 7 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3239 3839 844 Size: 2395 600 3489 Fragments arranged by size: 3489 2395 600 BsrBI CCG'CTC Cuts at: 1394 1823 2053 2085 3420 4371 1394 Size: 429 230 32 1335 951 3507 Fragments arranged by size: 3507 1335 951 429 230 32 BsrDI GCAATG_nn' Cuts at: 65 1248 2946 3273 3472 4979 5153 65 Size: 1183 1698 327 199 1507 174 1396 Fragments arranged by size: 1698 1507 1396 1183 327 199 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5128 5315 1700 Size: 110 3318 187 2869 Fragments arranged by size: 3318 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 4544 5556 4544 Size: 1012 5472 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 794 Size: 1445 5039 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 3055 3758 1184 Size: 1871 703 3910 Fragments arranged by size: 3910 1871 703 BstXI CCAn_nnnn'nTGG Cuts at: 3610 3610 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2706 2848 3414 3564 3814 4181 4566 4583 Size: 734 142 566 150 250 367 385 17 Cuts at: 4583 6035 6121 6133 6479 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 734 566 385 367 346 250 150 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2342 2630 2837 3488 2342 Size: 288 207 651 5338 Fragments arranged by size: 5338 651 288 207 BtgI C'CryG_G Cuts at: 513 1065 2004 2042 2317 2965 3603 513 Size: 552 939 38 275 648 638 3394 Fragments arranged by size: 3394 939 648 638 552 275 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 3768 4000 4794 4814 3768 Size: 232 794 20 5438 Fragments arranged by size: 5438 794 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3053 5491 809 Size: 507 1737 2438 1802 Fragments arranged by size: 2438 1802 1737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2717 2775 Size: 54 1425 50 275 18 313 574 58 Cuts at: 2775 3600 3931 4820 6449 8 Size: 825 331 889 1629 43 Fragments arranged by size: 1629 1425 889 825 574 331 313 275 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 3179 3785 4414 1353 Size: 1094 732 606 629 3423 Fragments arranged by size: 3423 1094 732 629 606 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2411 3110 5071 5445 5591 387 Size: 1064 138 822 699 1961 374 146 1280 Fragments arranged by size: 1961 1280 1064 822 699 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2799 2194 Size: 605 5879 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2177 2190 2213 2246 2462 2507 Size: 393 465 80 13 23 33 216 45 Cuts at: 2507 2585 2723 2747 2894 2996 3027 3201 3212 Size: 78 138 24 147 102 31 174 11 Cuts at: 3212 3333 3431 4547 5144 5931 6199 1239 Size: 121 98 1116 597 787 268 1524 Fragments arranged by size: 1524 1116 787 597 465 393 268 216 174 147 138 121 102 98 80 78 45 33 31 24 23 13 11 EcoNI CCTnn'n_nnAGG Cuts at: 2261 3346 2261 Size: 1085 5399 EcoO109I rG'GnC_Cy Cuts at: 2310 2375 2526 2670 2671 2842 3260 3391 3444 Size: 65 151 144 1 171 418 131 53 Cuts at: 3444 3492 3572 2310 Size: 48 80 5222 Fragments arranged by size: 5222 418 171 151 144 131 80 65 53 48 1 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2572 5631 1678 Size: 894 3059 2531 Fragments arranged by size: 3059 2531 894 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 3337 3369 3375 Size: 32 135 32 530 32 1203 32 6 Cuts at: 3375 3407 3734 3766 5125 5157 5199 5231 1373 Size: 32 327 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 1203 530 327 135 42 32 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 2719 2777 3602 10 Size: 54 1425 343 313 574 58 825 2892 Fragments arranged by size: 2892 1425 825 574 343 313 58 54 MslI CAynn'nnrTG Cuts at: 518 1166 3512 3554 3641 4442 4801 4960 518 Size: 648 2346 42 87 801 359 159 2042 Fragments arranged by size: 2346 2042 801 648 359 159 87 42 MspA1I CmG'CkG Cuts at: 1606 2094 2666 3900 4577 5725 5970 1606 Size: 488 572 1234 677 1148 245 2120 Fragments arranged by size: 2120 1234 1148 677 572 488 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2042 2317 2965 3603 513 Size: 552 939 38 275 648 638 3394 Fragments arranged by size: 3394 939 648 638 552 275 38 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 2539 1052 Size: 1487 4997 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2937 3145 3296 3842 5387 1132 Size: 97 1708 208 151 546 1545 2229 Fragments arranged by size: 2229 1708 1545 546 208 151 97 PasI CC'CwG_GG Cuts at: 1348 2741 3569 1348 Size: 1393 828 4263 Fragments arranged by size: 4263 1393 828 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2269 2485 2989 1108 Size: 1161 216 504 4603 Fragments arranged by size: 4603 1161 504 216 PfoI T'CCnGG_A Cuts at: 2196 2262 2844 2910 2988 3341 2196 Size: 66 582 66 78 353 5339 Fragments arranged by size: 5339 582 353 78 66 66 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2842 3391 3492 2842 Size: 549 101 5834 Fragments arranged by size: 5834 549 101 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2670 2670 Size: 6484 PstI C_TGCA'G Cuts at: 830 2352 3836 830 Size: 1522 1484 3478 Fragments arranged by size: 3478 1522 1484 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2094 2666 2094 Size: 572 5912 SacI G_AGCT'C Cuts at: 2196 2801 2196 Size: 605 5879 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 3179 3785 3179 Size: 606 5878 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SexAI A'CCwGG_T Cuts at: 2505 2505 Size: 6484 SfcI C'TryA_G Cuts at: 826 1045 2348 3832 4967 5648 5839 826 Size: 219 1303 1484 1135 681 191 1471 Fragments arranged by size: 1484 1471 1303 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2044 2044 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2300 2693 3061 4588 5489 5751 Size: 197 926 357 393 368 1527 901 262 Cuts at: 5751 820 Size: 1553 Fragments arranged by size: 1553 1527 926 901 393 368 357 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 2620 152 Size: 2468 4016 SphI G_CATG'C Cuts at: 1132 2937 3296 3842 1132 Size: 1805 359 546 3774 Fragments arranged by size: 3774 1805 546 359 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 2394 3352 1517 Size: 877 958 4649 Fragments arranged by size: 4649 958 877 StyI C'CwwG_G Cuts at: 513 1065 2004 2042 2317 2965 3603 3923 513 Size: 552 939 38 275 648 638 320 3074 Fragments arranged by size: 3074 939 648 638 552 320 275 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4511 4528 4681 4866 5311 325 Size: 1255 2931 17 153 185 445 1498 Fragments arranged by size: 2931 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3399 3431 6444 6476 3399 Size: 32 3013 32 3407 Fragments arranged by size: 3407 3013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2031 2963 3134 2031 Size: 932 171 5381 Fragments arranged by size: 5381 932 171 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI