Restriction map for pFN21AB9642
 (Linear) (Six-base) MAPSORT of: KIBB9642.seq  Check: 3510  from: 1  to: 1779



KIBB9642.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1234    1779 
   Size:       1234     545
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     991    1207    1779 
   Size:        991     216     572

  Fragments arranged by size: 

                 991     572     216
 
AfeI AGC'GCT

Cuts at:      0     565    1779 
   Size:        565    1214
 
AleI CACnn'nnGTG

Cuts at:      0    1636    1779 
   Size:       1636     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     586    1779 
   Size:        480     106    1193

  Fragments arranged by size: 

                1193     480     106
 
ApaI G_GGCC'C

Cuts at:      0     669    1779 
   Size:        669    1110
 
BamHI G'GATC_C

Cuts at:      0    1559    1779 
   Size:       1559     220
 
BanI G'GyrC_C

Cuts at:      0     798    1719    1779 
   Size:        798     921      60

  Fragments arranged by size: 

                 921     798      60
 
BanII G_rGCy'C

Cuts at:      0     191     224     669     755     796    1779 
   Size:        191      33     445      86      41     983

  Fragments arranged by size: 

                 983     445     191      86      41      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     372    1779 
   Size:        372    1407
 
BbvCI CC'TCA_GC

Cuts at:      0     271     574     823    1779 
   Size:        271     303     249     956

  Fragments arranged by size: 

                 956     303     271     249
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     334     368    1779 
   Size:        334      34    1411

  Fragments arranged by size: 

                1411     334      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      39    1215    1779 
   Size:         39    1176     564

  Fragments arranged by size: 

                1176     564      39
 
BlpI GC'TnA_GC

Cuts at:      0     886    1779 
   Size:        886     893
 
Bme1580I G_kGCm'C

Cuts at:      0     669     803    1220    1779 
   Size:        669     134     417     559

  Fragments arranged by size: 

                 669     559     417     134
 
BmtI G_CTAG'C

Cuts at:      0     538    1779 
   Size:        538    1241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1431    1779 
   Size:       1431     348
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     172     185     208     241     457     502     580 
   Size:         92      80      13      23      33     216      45      78

Cuts at:    580     718     742     889    1022    1196    1328    1426    1779 
   Size:        138      24     147     133     174     132      98     353

  Fragments arranged by size: 

                 353     216     174     147     138     133     132      98
                  92      80      78      45      33      24      23      13
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     247     271     574     823    1779 
   Size:        247      24     303     249     956

  Fragments arranged by size: 

                 956     303     249     247      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     673    1077    1779 
   Size:        316     357     404     702

  Fragments arranged by size: 

                 702     404     357     316
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1310    1779 
   Size:       1310     469
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      97     127    1779 
   Size:         97      30    1652

  Fragments arranged by size: 

                1652      97      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49      52    1087    1291    1779 
   Size:         49       3    1035     204     488

  Fragments arranged by size: 

                1035     488     204      49       3
 
BseYI C'CCAG_C

Cuts at:      0     116     661     678     873    1320    1362    1372    1468 
   Size:        116     545      17     195     447      42      10      96

Cuts at:   1468    1779 
   Size:        311

  Fragments arranged by size: 

                 545     447     311     195     116      96      42      17
                  10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93    1441    1779 
   Size:         93    1348     338

  Fragments arranged by size: 

                1348     338      93
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     191     550     796    1779 
   Size:        134      57     359     246     983

  Fragments arranged by size: 

                 983     359     246     134      57
 
BsmI GAATG_Cn'

Cuts at:      0     239    1779 
   Size:        239    1540
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     610    1779 
   Size:        610    1169
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     191     224     550     669     755     796     803 
   Size:        134      57      33     326     119      86      41       7

Cuts at:    803    1220    1779 
   Size:        417     559

  Fragments arranged by size: 

                 559     417     326     134     119      86      57      41
                  33       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1234    1779 
   Size:       1234     545
 
BsrBI CCG'CTC

Cuts at:      0      48      80    1415    1779 
   Size:         48      32    1335     364

  Fragments arranged by size: 

                1335     364      48      32
 
BsrDI GCAATG_nn'

Cuts at:      0     941    1268    1467    1779 
   Size:        941     327     199     312

  Fragments arranged by size: 

                 941     327     312     199
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     234    1779 
   Size:        234    1545
 
BstEII G'GTnAC_C

Cuts at:      0    1050    1753    1779 
   Size:       1050     703      26

  Fragments arranged by size: 

                1050     703      26
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1605    1779 
   Size:       1605     174
 
BstYI r'GATC_y

Cuts at:      0     701     843    1409    1559    1779 
   Size:        701     142     566     150     220

  Fragments arranged by size: 

                 701     566     220     150     142
 
Bsu36I CC'TnA_GG

Cuts at:      0     337     625     832    1483    1779 
   Size:        337     288     207     651     296

  Fragments arranged by size: 

                 651     337     296     288     207
 
BtgI C'CryG_G

Cuts at:      0      37     312     960    1598    1779 
   Size:         37     275     648     638     181

  Fragments arranged by size: 

                 648     638     275     181      37
 
BtsI GCAGTG_nn'

Cuts at:      0    1763    1779 
   Size:       1763      16
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1048    1779 
   Size:       1048     731
 
EaeI y'GGCC_r

Cuts at:      0     138     712     770    1595    1779 
   Size:        138     574      58     825     184

  Fragments arranged by size: 

                 825     574     184     138      58
 
EarI CTCTTCn'nnn_

Cuts at:      0     442    1174    1779 
   Size:        442     732     605

  Fragments arranged by size: 

                 732     605     442
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     406    1105    1779 
   Size:        406     699     674

  Fragments arranged by size: 

                 699     674     406
 
EcoICRI GAG'CTC

Cuts at:      0     189     794    1779 
   Size:        189     605     985

  Fragments arranged by size: 

                 985     605     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     172     185     208     241     457     502     580 
   Size:         92      80      13      23      33     216      45      78

Cuts at:    580     718     742     889     991    1022    1196    1207    1328 
   Size:        138      24     147     102      31     174      11     121

Cuts at:   1328    1426    1779 
   Size:         98     353

  Fragments arranged by size: 

                 353     216     174     147     138     121     102      98
                  92      80      78      45      33      31      24      23
                  13      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     256    1341    1779 
   Size:        256    1085     438

  Fragments arranged by size: 

                1085     438     256
 
EcoO109I rG'GnC_Cy

Cuts at:      0     305     370     521     665     666     837    1255    1386 
   Size:        305      65     151     144       1     171     418     131

Cuts at:   1386    1439    1487    1567    1779 
   Size:         53      48      80     212

  Fragments arranged by size: 

                 418     305     212     171     151     144     131      80
                  65      53      48       1
 
HaeII r_GCGC'y

Cuts at:      0     567    1779 
   Size:        567    1212
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129    1332    1364    1370    1402    1729    1761 
   Size:         97      32    1203      32       6      32     327      32

Cuts at:   1761    1779 
   Size:         18

  Fragments arranged by size: 

                1203     327      97      32      32      32      32      18
                   6
 
MscI TGG'CCA

Cuts at:      0     140     714     772    1597    1779 
   Size:        140     574      58     825     182

  Fragments arranged by size: 

                 825     574     182     140      58
 
MslI CAynn'nnrTG

Cuts at:      0    1507    1549    1636    1779 
   Size:       1507      42      87     143

  Fragments arranged by size: 

                1507     143      87      42
 
MspA1I CmG'CkG

Cuts at:      0      89     661    1779 
   Size:         89     572    1118

  Fragments arranged by size: 

                1118     572      89
 
NcoI C'CATG_G

Cuts at:      0      37     312     960    1598    1779 
   Size:         37     275     648     638     181

  Fragments arranged by size: 

                 648     638     275     181      37
 
NheI G'CTAG_C

Cuts at:      0     534    1779 
   Size:        534    1245
 
NspI r_CATG'y

Cuts at:      0     932    1140    1291    1779 
   Size:        932     208     151     488

  Fragments arranged by size: 

                 932     488     208     151
 
PasI CC'CwG_GG

Cuts at:      0     736    1564    1779 
   Size:        736     828     215

  Fragments arranged by size: 

                 828     736     215
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     264     480     984    1779 
   Size:        264     216     504     795

  Fragments arranged by size: 

                 795     504     264     216
 
PfoI T'CCnGG_A

Cuts at:      0     191     257     839     905     983    1336    1779 
   Size:        191      66     582      66      78     353     443

  Fragments arranged by size: 

                 582     443     353     191      78      66      66
 
PpuMI rG'GwC_Cy

Cuts at:      0     837    1386    1487    1779 
   Size:        837     549     101     292

  Fragments arranged by size: 

                 837     549     292     101
 
PspOMI G'GGCC_C

Cuts at:      0     665    1779 
   Size:        665    1114
 
PstI C_TGCA'G

Cuts at:      0     347    1779 
   Size:        347    1432
 
PvuII CAG'CTG

Cuts at:      0      89     661    1779 
   Size:         89     572    1118

  Fragments arranged by size: 

                1118     572      89
 
SacI G_AGCT'C

Cuts at:      0     191     796    1779 
   Size:        191     605     983

  Fragments arranged by size: 

                 983     605     191
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1174    1779 
   Size:       1174     605
 
SexAI A'CCwGG_T

Cuts at:      0     500    1779 
   Size:        500    1279
 
SfcI C'TryA_G

Cuts at:      0     343    1779 
   Size:        343    1436
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      39    1779 
   Size:         39    1740
 
SmlI C'TyrA_G

Cuts at:      0     295     688    1056    1779 
   Size:        295     393     368     723

  Fragments arranged by size: 

                 723     393     368     295
 
SpeI A'CTAG_T

Cuts at:      0     615    1779 
   Size:        615    1164
 
SphI G_CATG'C

Cuts at:      0     932    1291    1779 
   Size:        932     359     488

  Fragments arranged by size: 

                 932     488     359
 
StuI AGG'CCT

Cuts at:      0     389    1347    1779 
   Size:        389     958     432

  Fragments arranged by size: 

                 958     432     389
 
StyI C'CwwG_G

Cuts at:      0      37     312     960    1598    1779 
   Size:         37     275     648     638     181

  Fragments arranged by size: 

                 648     638     275     181      37
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1394    1426    1779 
   Size:       1394      32     353

  Fragments arranged by size: 

                1394     353      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      26     958    1129    1779 
   Size:         26     932     171     650

  Fragments arranged by size: 

                 932     650     171      26
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI    AlwNI     ApaI    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I     MscI     MslI   MspA1I     NcoI     NheI     NspI     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SfiI     SmlI     SpeI     SphI     StuI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI
    BspHI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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