(Circular) (Six-base) MAPSORT of: pFN21AB9611.seq Check: 6059 from: 1 to: 6472 pFN21AB9611 6472 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3771 3771 Size: 6472 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2148 4282 278 Size: 53 83 186 936 612 2134 2468 Fragments arranged by size: 2468 2134 936 612 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3356 3672 4535 5919 6187 1632 Size: 1724 316 863 1384 268 1917 Fragments arranged by size: 1917 1724 1384 863 316 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 3379 5201 3379 Size: 1822 4650 AleI CACnn'nnGTG Cuts at: 1166 3713 1166 Size: 2547 3925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2480 1184 Size: 1296 5176 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3050 3082 3050 Size: 32 6440 AlwNI CAG_nnn'CTG Cuts at: 2862 3464 3753 4215 5787 2862 Size: 602 289 462 1572 3547 Fragments arranged by size: 3547 1572 602 462 289 ApaLI G'TGCA_C Cuts at: 2525 4529 5685 2525 Size: 2004 1156 3312 Fragments arranged by size: 3312 2004 1156 ApoI r'AATT_y Cuts at: 1493 3509 3781 4007 4159 6268 1493 Size: 2016 272 226 152 2109 1697 Fragments arranged by size: 2109 2016 1697 272 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 2596 2693 3797 1870 Size: 73 653 97 1104 4545 Fragments arranged by size: 4545 1104 653 97 73 BamHI G'GATC_C Cuts at: 2829 3802 4169 2829 Size: 973 367 5132 Fragments arranged by size: 5132 973 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 3793 5248 618 Size: 325 217 627 1331 675 1455 1842 Fragments arranged by size: 1842 1455 1331 675 627 325 217 BanII G_rGCy'C Cuts at: 1391 2700 3053 3791 1391 Size: 1309 353 738 4072 Fragments arranged by size: 4072 1309 738 353 BbeI G_GCGC'C Cuts at: 3122 3122 Size: 6472 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2626 3202 928 Size: 971 727 576 4198 Fragments arranged by size: 4198 971 727 576 BbvCI CC'TCA_GC Cuts at: 3010 3010 Size: 6472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4663 4697 1903 Size: 34 2726 34 3678 Fragments arranged by size: 3678 2726 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 1548 Size: 6472 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2195 3524 5083 Size: 107 122 71 631 605 523 1329 1559 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 1559 1525 1329 631 605 523 122 107 71 BglII A'GATC_T Cuts at: 2931 3483 6467 2931 Size: 552 2984 2936 Fragments arranged by size: 2984 2936 552 BlpI GC'TnA_GC Cuts at: 3151 3156 3889 3151 Size: 5 733 5734 Fragments arranged by size: 5734 733 5 Bme1580I G_kGCm'C Cuts at: 1790 2529 2869 3440 3720 4533 5689 1790 Size: 739 340 571 280 813 1156 2573 Fragments arranged by size: 2573 1156 813 739 571 340 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6472 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5161 442 Size: 416 53 83 399 24 2786 958 1753 Fragments arranged by size: 2786 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 2444 2630 2633 2690 2740 3182 5132 Size: 1079 126 186 3 57 50 442 1950 Cuts at: 5132 1239 Size: 2579 Fragments arranged by size: 2579 1950 1079 442 186 126 57 50 3 Bpu10I CC'TnA_GC Cuts at: 3010 3010 Size: 6472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4597 5462 5760 4597 Size: 865 298 5309 Fragments arranged by size: 5309 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 2606 3070 3539 5135 882 Size: 617 519 588 464 469 1596 2219 Fragments arranged by size: 2219 1596 617 588 519 469 464 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 3488 6466 3488 Size: 2978 3494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2145 2475 3119 3303 Size: 53 83 186 936 612 330 644 184 Cuts at: 3303 4279 4661 5296 275 Size: 976 382 635 1451 Fragments arranged by size: 1451 976 936 644 635 612 382 330 186 184 83 53 BsaWI w'CCGG_w Cuts at: 4905 5303 5577 5724 6135 4905 Size: 398 274 147 411 5242 Fragments arranged by size: 5242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 3050 3080 3401 Size: 30 165 30 663 30 954 30 321 Cuts at: 3401 3431 1178 Size: 30 4219 Fragments arranged by size: 4219 954 663 321 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2314 2360 2690 2759 1186 Size: 1128 46 330 69 4899 Fragments arranged by size: 4899 1128 330 69 46 BseYI C'CCAG_C Cuts at: 2984 3441 5675 6171 2984 Size: 457 2234 496 3285 Fragments arranged by size: 3285 2234 496 457 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2267 1163 Size: 573 531 5368 Fragments arranged by size: 5368 573 531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 3018 3922 4683 5711 1540 Size: 275 186 166 851 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 851 761 275 186 166 BsiHKAI G_wGCw'C Cuts at: 2529 2700 4533 4618 5689 2529 Size: 171 1833 85 1071 3312 Fragments arranged by size: 3312 1833 1071 171 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 BsmBI CGTCTCn'nnnn_ Cuts at: 2528 2758 2528 Size: 230 6242 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 2700 2869 3053 3440 3720 3791 Size: 399 739 171 169 184 387 280 71 Cuts at: 3791 4533 4618 5689 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 399 387 280 184 171 169 85 71 BspEI T'CCGG_A Cuts at: 6135 6135 Size: 6472 BspHI T'CATG_A Cuts at: 4361 6091 4361 Size: 1730 4742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2289 2988 3771 3827 844 Size: 1445 699 783 56 3489 Fragments arranged by size: 3489 1445 783 699 56 BsrBI CCG'CTC Cuts at: 1394 1823 2626 4359 1394 Size: 429 803 1733 3507 Fragments arranged by size: 3507 1733 803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3316 4967 5141 65 Size: 1183 2068 1651 174 1396 Fragments arranged by size: 2068 1651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 5116 5303 1700 Size: 110 680 2626 187 2869 Fragments arranged by size: 2869 2626 680 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6472 BssSI C'ACGA_G Cuts at: 4532 5544 4532 Size: 1012 5460 BstAPI GCAn_nnn'nTGC Cuts at: 794 2130 3594 794 Size: 1336 1464 3672 Fragments arranged by size: 3672 1464 1336 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 2851 1184 Size: 1667 4805 BstXI CCAn_nnnn'nTGG Cuts at: 2452 2452 Size: 6472 BstYI r'GATC_y Cuts at: 1972 2829 2931 3483 3802 4169 4554 4571 6023 Size: 857 102 552 319 367 385 17 1452 Cuts at: 6023 6109 6121 6467 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 857 552 385 367 346 319 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2233 2675 3145 3735 3768 513 Size: 552 1168 442 470 590 33 3217 Fragments arranged by size: 3217 1168 590 552 470 442 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2317 487 Size: 683 56 1091 4642 Fragments arranged by size: 4642 1091 683 56 BtsI GCAGTG_nn' Cuts at: 2128 2579 3324 3988 4782 4802 2128 Size: 451 745 664 794 20 3798 Fragments arranged by size: 3798 794 745 664 451 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3724 3759 529 Size: 35 3160 35 3242 Fragments arranged by size: 3242 3160 35 35 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DraIII CAC_nnn'GTG Cuts at: 2398 2398 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2032 5479 809 Size: 507 716 3447 1802 Fragments arranged by size: 3447 1802 716 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2870 3142 Size: 54 1425 50 275 18 366 674 272 Cuts at: 3142 3919 4808 6437 8 Size: 777 889 1629 43 Fragments arranged by size: 1629 1425 889 777 674 366 275 272 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3919 1537 Size: 275 2107 4090 Fragments arranged by size: 4090 2107 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 4402 1353 Size: 722 2327 3423 Fragments arranged by size: 3423 2327 722 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5059 5433 5579 387 Size: 1064 138 3470 374 146 1280 Fragments arranged by size: 3470 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2698 2698 Size: 6472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 2444 2630 2633 2690 2740 3182 Size: 393 686 126 186 3 57 50 442 Cuts at: 3182 3356 3672 4535 5132 5919 6187 1239 Size: 174 316 863 597 787 268 1524 Fragments arranged by size: 1524 863 787 686 597 442 393 316 268 186 174 126 57 50 3 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2744 2570 Size: 174 6298 EcoO109I rG'GnC_Cy Cuts at: 2764 2809 3648 2764 Size: 45 839 5588 Fragments arranged by size: 5588 839 45 EcoRI G'AATT_C Cuts at: 3509 3781 4159 3509 Size: 272 378 5822 Fragments arranged by size: 5822 378 272 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 6472 FspI TGC'GCA Cuts at: 4978 4978 Size: 6472 HaeII r_GCGC'y Cuts at: 1678 3009 3122 5619 1678 Size: 1331 113 2497 2531 Fragments arranged by size: 2531 2497 1331 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2579 2611 3092 3124 3365 Size: 32 135 32 1007 32 481 32 241 Cuts at: 3365 3397 5113 5145 5187 5219 1373 Size: 32 1716 32 42 32 2626 Fragments arranged by size: 2626 1716 1007 481 241 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2266 3816 4062 669 Size: 988 281 328 1550 246 3079 Fragments arranged by size: 3079 1550 988 328 281 246 HindIII A'AGCT_T Cuts at: 748 2792 748 Size: 2044 4428 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KasI G'GCGC_C Cuts at: 3118 3118 Size: 6472 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 2469 2757 5586 5770 1305 Size: 1041 123 288 2829 184 2007 Fragments arranged by size: 2829 2007 1041 288 184 123 MscI TGG'CCA Cuts at: 10 64 1489 1832 3144 10 Size: 54 1425 343 1312 3338 Fragments arranged by size: 3338 1425 1312 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4430 4789 4948 518 Size: 648 2547 717 359 159 2042 Fragments arranged by size: 2547 2042 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3155 3464 3770 3888 4565 5713 5958 1606 Size: 1549 309 306 118 677 1148 245 2120 Fragments arranged by size: 2120 1549 1148 677 309 306 245 118 NaeI GCC'GGC Cuts at: 2492 2492 Size: 6472 NarI GG'CG_CC Cuts at: 3119 3119 Size: 6472 NcoI C'CATG_G Cuts at: 513 1065 2233 3145 513 Size: 552 1168 912 3840 Fragments arranged by size: 3840 1168 912 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NgoMIV G'CCGG_C Cuts at: 2490 2490 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3919 3919 Size: 6472 NspI r_CATG'y Cuts at: 1132 1229 2137 2383 2518 3592 3830 5375 1132 Size: 97 908 246 135 1074 238 1545 2229 Fragments arranged by size: 2229 1545 1074 908 246 238 135 97 PasI CC'CwG_GG Cuts at: 1348 3260 1348 Size: 1912 4560 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 3273 3555 3604 1108 Size: 2165 282 49 3976 Fragments arranged by size: 3976 2165 282 49 PfoI T'CCnGG_A Cuts at: 2271 2421 2271 Size: 150 6322 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2210 2242 3050 3082 4541 4573 6054 6086 2210 Size: 32 808 32 1459 32 1481 32 2596 Fragments arranged by size: 2596 1481 1459 808 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3648 3648 Size: 6472 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PstI C_TGCA'G Cuts at: 830 2388 2745 2838 3824 830 Size: 1558 357 93 986 3478 Fragments arranged by size: 3478 1558 986 357 93 PvuI CG_AT'CG Cuts at: 2001 3018 2001 Size: 1017 5455 PvuII CAG'CTG Cuts at: 3155 3155 Size: 6472 SacI G_AGCT'C Cuts at: 2700 2700 Size: 6472 SacII CC_GC'GG Cuts at: 3771 3771 Size: 6472 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SanDI GG'GwC_CC Cuts at: 3648 3648 Size: 6472 SbfI CC_TGCA'GG Cuts at: 2745 3824 2745 Size: 1079 5393 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SexAI A'CCwGG_T Cuts at: 2937 2937 Size: 6472 SfcI C'TryA_G Cuts at: 826 1045 2384 2741 2834 3820 4955 5636 5827 Size: 219 1339 357 93 986 1135 681 191 Cuts at: 5827 826 Size: 1471 Fragments arranged by size: 1471 1339 1135 986 681 357 219 191 93 SfoI GGC'GCC Cuts at: 3120 3120 Size: 6472 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3799 1872 Size: 1927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2596 4576 5477 5739 820 Size: 197 926 653 1980 901 262 1553 Fragments arranged by size: 1980 1553 926 901 653 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 2081 1517 Size: 564 5908 StyI C'CwwG_G Cuts at: 513 1065 2233 2571 2955 2970 3069 3111 3145 Size: 552 1168 338 384 15 99 42 34 Cuts at: 3145 3384 3446 3911 513 Size: 239 62 465 3074 Fragments arranged by size: 3074 1168 552 465 384 338 239 99 62 42 34 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1999 2630 2644 3070 4499 4516 4669 Size: 1255 419 631 14 426 1429 17 153 Cuts at: 4669 4854 5299 325 Size: 185 445 1498 Fragments arranged by size: 1498 1429 1255 631 445 426 419 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4718 6430 96 Size: 274 80 33 51 1443 2741 1712 138 Fragments arranged by size: 2741 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 2667 2699 3401 3433 6432 6464 2066 Size: 32 569 32 702 32 2999 32 2074 Fragments arranged by size: 2999 2074 702 569 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2261 1316 Size: 945 5527 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2797 2797 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 2596 1943 Size: 653 5819 XmaI C'CCGG_G Cuts at: 1870 3797 1870 Size: 1927 4545 XmnI GAAnn'nnTTC Cuts at: 2435 2999 4601 2435 Size: 564 1602 4306 Fragments arranged by size: 4306 1602 564 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2146 4280 276 Size: 53 83 186 936 612 2134 2468 Fragments arranged by size: 2468 2134 936 612 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SfiI SrfI SwaI