################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 457 GTCTAC GT^[AC][GT]AC AcyI 31 GACGCC G[GA]^CG[CT]C AfaI 253 GTAC GT^AC ApaI 514 GGGCCC GGGCC^C AspI 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 59 GGACC G^G[AT]CC AviII 605 TGCGCA TGC^GCA BbsI 448 GAAGAC GAAGAC(2/6) BbvCI 144 CCTCAGC CCTCAGC(-5/-2) BcnI 480 CCGGG CC^[GC]GG BpmI 22 CTGGAG CTGGAG(16/14) BpuAI 448 GAAGAC GAAGAC(2/6) BsaHI 31 GACGCC G[GA]^CG[CT]C BspMI 497 ACCTGC ACCTGC(4/8) BsrBI 96 CCGCTC CCGCTC(-3/-3) BsrDI 317 GCAATG GCAATG(2/0) BssSI 397 CTCGTG CACGAG(-5/-1) BstF5I 487 CATCC GGATG(2/0) EaeI 102 CGGCCA [CT]^GGCC[GA] EarI 648 GAAGAG CTCTTC(1/4) EcoRI 219 GAATTC G^AATTC FauI 95 CCCGC CCCGC(4/6) FokI 487 CATCC GGATG(9/13) FspI 605 TGCGCA TGC^GCA HgaI 31 GACGC GACGC(5/10) Hin1I 31 GACGCC G[GA]^CG[CT]C HphI 50 TCACC GGTGA(8/7) Ksp632I 648 GAAGAG CTCTTC(1/4) MseI 248 TTAA T^TAA NciI 480 CCGGG CC^[GC]GG NlaIII 553 CATG CATG^ NlaIV 514 GGGCCC GG[ACGT]^[ACGT]CC PflFI 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 289 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 58 AGGACCT [GA]G^G[AT]CC[CT] PstI 637 CTGCAG CTGCA^G PvuII 634 CAGCTG CAG^CTG RsaI 253 GTAC GT^AC SfaNI 608 GCATC GCATC(5/9) TaqI 374 TCGA T^CGA Tru9I 248 TTAA T^TAA Tth111I 451 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 374 TCGA T^CGA Van91I 289 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 640 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 673 CAGAAGCTG BanII 408 GAGCCC G[GA]GC[CT]^C 514 GGGCCC BmyI 408 GAGCCC G[AGT]GC[ACT]^C 514 GGGCCC BseRI 25 GAGGAG GAGGAG(10/8) 154 GAGGAG Bsp1286I 408 GAGCCC G[AGT]GC[ACT]^C 514 GGGCCC BsrI 238 ACTGG ACTGG(1/-1) 311 CCAGT DpnII 159 GATC ^GATC 372 GATC HaeII 78 AGCGCC [GA]GCGC^[CT] 653 GGCGCT HapII 101 CCGG C^CGG 480 CCGG HpaII 101 CCGG C^CGG 480 CCGG MboI 159 GATC ^GATC 372 GATC MspA1I 6 CAGCGG C[AC]G^C[GT]G 634 CAGCTG MspI 101 CCGG C^CGG 480 CCGG NdeII 159 GATC ^GATC 372 GATC Sau3AI 159 GATC ^GATC 372 GATC TspRI 237 CACTG CA[GC]TG[ACGT][ACGT]^ 357 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoNI EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI MaeIII MboII MnlI MvaI MwoI Sau96I ScrFI SfcI TseI Tsp45I Tsp509I