(Circular) (Six-base) MAPSORT of: pFN21AB5768.seq Check: 7350 from: 1 to: 6985 pFN21AB5768 6985 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4795 278 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3981 4080 4328 1937 Size: 2044 99 248 4594 Fragments arranged by size: 4594 2044 248 99 Acc65I G'GTAC_C Cuts at: 4306 4306 Size: 6985 AclI AA'CG_TT Cuts at: 1563 5112 5485 1563 Size: 3549 373 3063 Fragments arranged by size: 3549 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2156 2956 5048 6432 6700 1632 Size: 495 29 800 2092 1384 268 1917 Fragments arranged by size: 2092 1917 1384 800 495 268 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6788 AflIII A'CryG_T Cuts at: 1203 3972 5884 6861 1203 Size: 2769 1912 977 1327 Fragments arranged by size: 2769 1912 1327 977 AgeI A'CCGG_T Cuts at: 5816 5816 Size: 6985 AhdI GACnn_n'nnGTC Cuts at: 5714 5714 Size: 6985 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6985 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3415 1184 Size: 2231 4754 AlwNI CAG_nnn'CTG Cuts at: 4728 6300 4728 Size: 1572 5413 ApaLI G'TGCA_C Cuts at: 4118 5042 6198 4118 Size: 924 1156 4905 Fragments arranged by size: 4905 1156 924 ApoI r'AATT_y Cuts at: 1493 2420 2660 2678 3245 3821 4039 4294 4520 Size: 927 240 18 567 576 218 255 226 Cuts at: 4520 4672 6781 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 927 576 567 255 240 226 218 152 18 AseI AT'TA_AT Cuts at: 160 1032 5539 160 Size: 872 4507 1606 Fragments arranged by size: 4507 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 AvaI C'yCGr_G Cuts at: 1870 1943 4310 1870 Size: 73 2367 4545 Fragments arranged by size: 4545 2367 73 BamHI G'GATC_C Cuts at: 4315 4682 4315 Size: 367 6618 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3345 4306 5761 618 Size: 325 217 627 1558 961 1455 1842 Fragments arranged by size: 1842 1558 1455 961 627 325 217 BanII G_rGCy'C Cuts at: 1391 4304 1391 Size: 2913 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2985 3804 928 Size: 971 116 970 819 4109 Fragments arranged by size: 4109 971 970 819 116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5176 5210 1903 Size: 34 3239 34 3678 Fragments arranged by size: 3678 3239 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3615 3878 4877 6087 3615 Size: 263 999 1210 4513 Fragments arranged by size: 4513 1210 999 263 BclI T'GATC_A Cuts at: 1548 3448 1548 Size: 1900 5085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5596 136 Size: 107 122 71 631 605 3924 1525 Fragments arranged by size: 3924 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6980 6980 Size: 6985 BlpI GC'TnA_GC Cuts at: 4402 4402 Size: 6985 Bme1580I G_kGCm'C Cuts at: 1790 4122 5046 6202 1790 Size: 2332 924 1156 2573 Fragments arranged by size: 2573 2332 1156 924 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6985 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 4716 5674 Size: 416 53 83 399 24 1191 2108 958 Cuts at: 5674 442 Size: 1753 Fragments arranged by size: 2108 1753 1191 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2790 3933 3940 5645 1239 Size: 1551 1143 7 1705 2579 Fragments arranged by size: 2579 1705 1551 1143 7 Bpu10I CC'TnA_GC Cuts at: 2408 2408 Size: 6985 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2851 4046 5110 5975 6273 2851 Size: 1195 1064 865 298 3563 Fragments arranged by size: 3563 1195 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2652 4007 5648 882 Size: 617 1153 1355 1641 2219 Fragments arranged by size: 2219 1641 1355 1153 617 BsaAI yAC'GTr Cuts at: 493 1204 2895 4776 493 Size: 711 1691 1881 2702 Fragments arranged by size: 2702 1881 1691 711 BsaBI GATnn'nnATC Cuts at: 4020 6979 4020 Size: 2959 4026 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4792 5174 5809 275 Size: 53 83 186 936 3259 382 635 1451 Fragments arranged by size: 3259 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5418 5816 6090 6237 6648 5418 Size: 398 274 147 411 5755 Fragments arranged by size: 5755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2409 2439 3873 3903 1178 Size: 30 165 30 1006 30 1434 30 4260 Fragments arranged by size: 4260 1434 1006 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3570 3746 1186 Size: 2384 176 4425 Fragments arranged by size: 4425 2384 176 BseYI C'CCAG_C Cuts at: 3778 3960 6188 6684 3778 Size: 182 2228 496 4079 Fragments arranged by size: 4079 2228 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6412 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4435 5196 6224 1540 Size: 275 186 2434 761 1028 2301 Fragments arranged by size: 2434 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 4032 4122 5046 5131 6202 2128 Size: 1904 90 924 85 1071 2911 Fragments arranged by size: 2911 1904 1071 924 90 85 BsmI GAATG_Cn' Cuts at: 2305 4496 4589 2305 Size: 2191 93 4701 Fragments arranged by size: 4701 2191 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 4032 4122 4304 5046 5131 6202 Size: 399 338 1904 90 182 742 85 1071 Cuts at: 6202 1391 Size: 2174 Fragments arranged by size: 2174 1904 1071 742 399 338 182 90 85 BspEI T'CCGG_A Cuts at: 6648 6648 Size: 6985 BspHI T'CATG_A Cuts at: 3433 4874 6604 3433 Size: 1441 1730 3814 Fragments arranged by size: 3814 1730 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4340 844 Size: 3496 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4872 1394 Size: 429 3049 3507 Fragments arranged by size: 3507 3049 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 2862 3247 3708 4188 5480 5654 Size: 1183 1171 443 385 461 480 1292 174 Cuts at: 5654 65 Size: 1396 Fragments arranged by size: 1396 1292 1183 1171 480 461 443 385 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5629 5816 1700 Size: 110 3819 187 2869 Fragments arranged by size: 3819 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6985 BssSI C'ACGA_G Cuts at: 5045 6057 5045 Size: 1012 5973 BstAPI GCAn_nnn'nTGC Cuts at: 794 4142 794 Size: 3348 3637 BstBI TT'CG_AA Cuts at: 5789 5789 Size: 6985 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6985 BstYI r'GATC_y Cuts at: 1972 2536 4315 4682 5067 5084 6536 6622 6634 Size: 564 1779 367 385 17 1452 86 12 Cuts at: 6634 6980 1972 Size: 346 1977 Fragments arranged by size: 1977 1779 1452 564 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4096 4204 513 Size: 552 3031 108 3294 Fragments arranged by size: 3294 3031 552 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2739 487 Size: 683 56 1513 4733 Fragments arranged by size: 4733 1513 683 56 BtsI GCAGTG_nn' Cuts at: 2930 3771 3948 4271 4501 5295 5315 2930 Size: 841 177 323 230 794 20 4600 Fragments arranged by size: 4600 841 794 323 230 177 20 ClaI AT'CG_AT Cuts at: 1554 2805 1554 Size: 1251 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6950 DraI TTT'AAA Cuts at: 2148 4289 2148 Size: 2141 4844 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5992 809 Size: 507 4676 1802 Fragments arranged by size: 4676 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4432 5321 6950 Size: 54 1425 50 275 18 2602 889 1629 Cuts at: 6950 8 Size: 43 Fragments arranged by size: 2602 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4432 1537 Size: 275 2620 4090 Fragments arranged by size: 4090 2620 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2560 2604 4915 1353 Size: 1207 44 2311 3423 Fragments arranged by size: 3423 2311 1207 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5572 5946 6092 387 Size: 1064 138 3983 374 146 1280 Fragments arranged by size: 3983 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2156 2790 2956 3933 3940 5048 Size: 393 495 29 634 166 977 7 1108 Cuts at: 5048 5645 6432 6700 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1108 977 787 634 597 495 393 268 166 29 7 EcoO109I rG'GnC_Cy Cuts at: 2831 2831 Size: 6985 EcoRI G'AATT_C Cuts at: 4039 4294 4672 4039 Size: 255 378 6352 Fragments arranged by size: 6352 378 255 EcoRV GAT'ATC Cuts at: 3678 3678 Size: 6985 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2222 3625 2222 Size: 1403 5582 FspI TGC'GCA Cuts at: 5491 5491 Size: 6985 HaeII r_GCGC'y Cuts at: 1678 6132 1678 Size: 4454 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3562 3594 3667 3699 5626 Size: 32 135 32 1990 32 73 32 1927 Cuts at: 5626 5658 5700 5732 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1990 1927 135 73 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4329 4575 669 Size: 988 281 2391 246 3079 Fragments arranged by size: 3079 2391 988 281 246 HindIII A'AGCT_T Cuts at: 748 2363 748 Size: 1615 5370 HpaI GTT'AAC Cuts at: 4575 4575 Size: 6985 KpnI G_GTAC'C Cuts at: 4310 4310 Size: 6985 MfeI C'AATT_G Cuts at: 4584 4584 Size: 6985 MluI A'CGCG_T Cuts at: 3972 6861 3972 Size: 2889 4096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6099 6283 1305 Size: 4794 184 2007 Fragments arranged by size: 4794 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5163 Fragments arranged by size: 5163 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3830 4244 4943 5302 5461 518 Size: 648 2664 414 699 359 159 2042 Fragments arranged by size: 2664 2042 699 648 414 359 159 MspA1I CmG'CkG Cuts at: 1606 3511 3812 3960 4401 5078 6226 6471 1606 Size: 1905 301 148 441 677 1148 245 2120 Fragments arranged by size: 2120 1905 1148 677 441 301 245 148 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6433 NdeI CA'TA_TG Cuts at: 387 2494 2671 3863 387 Size: 2107 177 1192 3509 Fragments arranged by size: 3509 2107 1192 177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6985 NotI GC'GGCC_GC Cuts at: 4432 4432 Size: 6985 NspI r_CATG'y Cuts at: 1132 1229 4343 5888 1132 Size: 97 3114 1545 2229 Fragments arranged by size: 3114 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6985 PciI A'CATG_T Cuts at: 5884 5884 Size: 6985 PflMI CCAn_nnn'nTGG Cuts at: 1108 2768 1108 Size: 1660 5325 PfoI T'CCnGG_A Cuts at: 3465 3465 Size: 6985 PmeI GTTT'AAAC Cuts at: 4289 4289 Size: 6985 PmlI CAC'GTG Cuts at: 2895 2895 Size: 6985 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5054 5086 6567 6599 5054 Size: 32 1481 32 5440 Fragments arranged by size: 5440 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2831 2831 Size: 6985 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6985 PsiI TTA'TAA Cuts at: 2166 2241 4555 2166 Size: 75 2314 4596 Fragments arranged by size: 4596 2314 75 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4220 4252 4220 Size: 32 6953 PstI C_TGCA'G Cuts at: 830 2221 3641 4337 830 Size: 1391 1420 696 3478 Fragments arranged by size: 3478 1420 1391 696 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6985 PvuII CAG'CTG Cuts at: 3511 3812 3960 3511 Size: 301 148 6536 Fragments arranged by size: 6536 301 148 SalI G'TCGA_C Cuts at: 1936 4327 1936 Size: 2391 4594 SbfI CC_TGCA'GG Cuts at: 4337 4337 Size: 6985 ScaI AGT'ACT Cuts at: 2235 3386 5233 6945 2235 Size: 1151 1847 1712 2275 Fragments arranged by size: 2275 1847 1712 1151 SfcI C'TryA_G Cuts at: 826 1045 2217 3277 3637 4333 5468 6149 6340 Size: 219 1172 1060 360 696 1135 681 191 Cuts at: 6340 826 Size: 1471 Fragments arranged by size: 1471 1172 1135 1060 696 681 360 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6985 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6985 SmaI CCC'GGG Cuts at: 1872 4312 1872 Size: 2440 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2866 4025 5089 5990 6252 820 Size: 197 926 923 1159 1064 901 262 1553 Fragments arranged by size: 1553 1159 1064 926 923 901 262 197 SnaBI TAC'GTA Cuts at: 493 4776 493 Size: 4283 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6985 SphI G_CATG'C Cuts at: 1132 4343 1132 Size: 3211 3774 SspI AAT'ATT Cuts at: 5 52 2245 2580 2752 3105 3353 3968 4067 Size: 47 2193 335 172 353 248 615 99 Cuts at: 4067 4909 5 Size: 842 2081 Fragments arranged by size: 2193 2081 842 615 353 335 248 172 99 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6985 StyI C'CwwG_G Cuts at: 513 1065 3714 3742 4424 513 Size: 552 2649 28 682 3074 Fragments arranged by size: 3074 2649 682 552 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2941 3770 4256 5012 5029 5182 5367 Size: 1255 1361 829 486 756 17 153 185 Cuts at: 5367 5812 325 Size: 445 1498 Fragments arranged by size: 1498 1361 1255 829 756 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2233 3384 5231 Size: 274 80 33 51 1443 256 1151 1847 Cuts at: 5231 6943 96 Size: 1712 138 Fragments arranged by size: 1847 1712 1443 1151 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4196 4228 6945 6977 4196 Size: 32 2717 32 4204 Fragments arranged by size: 4204 2717 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6985 XbaI T'CTAG_A Cuts at: 2344 4321 2344 Size: 1977 5008 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3957 3957 Size: 6985 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6985 XmaI C'CCGG_G Cuts at: 1870 4310 1870 Size: 2440 4545 XmnI GAAnn'nnTTC Cuts at: 2158 2205 5114 2158 Size: 47 2909 4029 Fragments arranged by size: 4029 2909 47 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4793 276 Size: 53 83 186 936 3259 2468 Fragments arranged by size: 3259 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI