Restriction map for pFN21AB5768
 (Circular) (Six-base) MAPSORT of: pFN21AB5768.seq  Check: 7350  from: 1  to: 6985



pFN21AB5768  6985 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:24  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4795     278 
   Size:         53      83     186     936    3259    2468

  Fragments arranged by size: 

                3259    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3981    4080    4328    1937 
   Size:       2044      99     248    4594

  Fragments arranged by size: 

                4594    2044     248      99
 
Acc65I G'GTAC_C

Cuts at:   4306    4306 
   Size:       6985
 
AclI AA'CG_TT

Cuts at:   1563    5112    5485    1563 
   Size:       3549     373    3063

  Fragments arranged by size: 

                3549    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2127    2156    2956    5048    6432    6700    1632 
   Size:        495      29     800    2092    1384     268    1917

  Fragments arranged by size: 

                2092    1917    1384     800     495     268      29
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6788
 
AflIII A'CryG_T

Cuts at:   1203    3972    5884    6861    1203 
   Size:       2769    1912     977    1327

  Fragments arranged by size: 

                2769    1912    1327     977
 
AgeI A'CCGG_T

Cuts at:   5816    5816 
   Size:       6985
 
AhdI GACnn_n'nnGTC

Cuts at:   5714    5714 
   Size:       6985
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6985
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3415    1184 
   Size:       2231    4754
 
AlwNI CAG_nnn'CTG

Cuts at:   4728    6300    4728 
   Size:       1572    5413
 
ApaLI G'TGCA_C

Cuts at:   4118    5042    6198    4118 
   Size:        924    1156    4905

  Fragments arranged by size: 

                4905    1156     924
 
ApoI r'AATT_y

Cuts at:   1493    2420    2660    2678    3245    3821    4039    4294    4520 
   Size:        927     240      18     567     576     218     255     226

Cuts at:   4520    4672    6781    1493 
   Size:        152    2109    1697

  Fragments arranged by size: 

                2109    1697     927     576     567     255     240     226
                 218     152      18
 
AseI AT'TA_AT

Cuts at:    160    1032    5539     160 
   Size:        872    4507    1606

  Fragments arranged by size: 

                4507    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6985
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4310    1870 
   Size:         73    2367    4545

  Fragments arranged by size: 

                4545    2367      73
 
BamHI G'GATC_C

Cuts at:   4315    4682    4315 
   Size:        367    6618
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3345    4306    5761     618 
   Size:        325     217     627    1558     961    1455    1842

  Fragments arranged by size: 

                1842    1558    1455     961     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    4304    1391 
   Size:       2913    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2015    2985    3804     928 
   Size:        971     116     970     819    4109

  Fragments arranged by size: 

                4109     971     970     819     116
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5176    5210    1903 
   Size:         34    3239      34    3678

  Fragments arranged by size: 

                3678    3239      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3615    3878    4877    6087    3615 
   Size:        263     999    1210    4513

  Fragments arranged by size: 

                4513    1210     999     263
 
BclI T'GATC_A

Cuts at:   1548    3448    1548 
   Size:       1900    5085
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5596     136 
   Size:        107     122      71     631     605    3924    1525

  Fragments arranged by size: 

                3924    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6980    6980 
   Size:       6985
 
BlpI GC'TnA_GC

Cuts at:   4402    4402 
   Size:       6985
 
Bme1580I G_kGCm'C

Cuts at:   1790    4122    5046    6202    1790 
   Size:       2332     924    1156    2573

  Fragments arranged by size: 

                2573    2332    1156     924
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6985
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2608    4716    5674 
   Size:        416      53      83     399      24    1191    2108     958

Cuts at:   5674     442 
   Size:       1753

  Fragments arranged by size: 

                2108    1753    1191     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6985
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2790    3933    3940    5645    1239 
   Size:       1551    1143       7    1705    2579

  Fragments arranged by size: 

                2579    1705    1551    1143       7
 
Bpu10I CC'TnA_GC

Cuts at:   2408    2408 
   Size:       6985
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2851    4046    5110    5975    6273    2851 
   Size:       1195    1064     865     298    3563

  Fragments arranged by size: 

                3563    1195    1064     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2652    4007    5648     882 
   Size:        617    1153    1355    1641    2219

  Fragments arranged by size: 

                2219    1641    1355    1153     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2895    4776     493 
   Size:        711    1691    1881    2702

  Fragments arranged by size: 

                2702    1881    1691     711
 
BsaBI GATnn'nnATC

Cuts at:   4020    6979    4020 
   Size:       2959    4026
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4792    5174    5809     275 
   Size:         53      83     186     936    3259     382     635    1451

  Fragments arranged by size: 

                3259    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5418    5816    6090    6237    6648    5418 
   Size:        398     274     147     411    5755

  Fragments arranged by size: 

                5755     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2409    2439    3873    3903    1178 
   Size:         30     165      30    1006      30    1434      30    4260

  Fragments arranged by size: 

                4260    1434    1006     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3570    3746    1186 
   Size:       2384     176    4425

  Fragments arranged by size: 

                4425    2384     176
 
BseYI C'CCAG_C

Cuts at:   3778    3960    6188    6684    3778 
   Size:        182    2228     496    4079

  Fragments arranged by size: 

                4079    2228     496     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    6412
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4435    5196    6224    1540 
   Size:        275     186    2434     761    1028    2301

  Fragments arranged by size: 

                2434    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2128    4032    4122    5046    5131    6202    2128 
   Size:       1904      90     924      85    1071    2911

  Fragments arranged by size: 

                2911    1904    1071     924      90      85
 
BsmI GAATG_Cn'

Cuts at:   2305    4496    4589    2305 
   Size:       2191      93    4701

  Fragments arranged by size: 

                4701    2191      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2128    4032    4122    4304    5046    5131    6202 
   Size:        399     338    1904      90     182     742      85    1071

Cuts at:   6202    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1904    1071     742     399     338     182      90
                  85
 
BspEI T'CCGG_A

Cuts at:   6648    6648 
   Size:       6985
 
BspHI T'CATG_A

Cuts at:   3433    4874    6604    3433 
   Size:       1441    1730    3814

  Fragments arranged by size: 

                3814    1730    1441
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    4340     844 
   Size:       3496    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4872    1394 
   Size:        429    3049    3507

  Fragments arranged by size: 

                3507    3049     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2419    2862    3247    3708    4188    5480    5654 
   Size:       1183    1171     443     385     461     480    1292     174

Cuts at:   5654      65 
   Size:       1396

  Fragments arranged by size: 

                1396    1292    1183    1171     480     461     443     385
                 174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5629    5816    1700 
   Size:        110    3819     187    2869

  Fragments arranged by size: 

                3819    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6985
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6985
 
BssSI C'ACGA_G

Cuts at:   5045    6057    5045 
   Size:       1012    5973
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    4142     794 
   Size:       3348    3637
 
BstBI TT'CG_AA

Cuts at:   5789    5789 
   Size:       6985
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6985
 
BstYI r'GATC_y

Cuts at:   1972    2536    4315    4682    5067    5084    6536    6622    6634 
   Size:        564    1779     367     385      17    1452      86      12

Cuts at:   6634    6980    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1779    1452     564     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    4096    4204     513 
   Size:        552    3031     108    3294

  Fragments arranged by size: 

                3294    3031     552     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2739     487 
   Size:        683      56    1513    4733

  Fragments arranged by size: 

                4733    1513     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2930    3771    3948    4271    4501    5295    5315    2930 
   Size:        841     177     323     230     794      20    4600

  Fragments arranged by size: 

                4600     841     794     323     230     177      20
 
ClaI AT'CG_AT

Cuts at:   1554    2805    1554 
   Size:       1251    5734
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6950
 
DraI TTT'AAA

Cuts at:   2148    4289    2148 
   Size:       2141    4844
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5992     809 
   Size:        507    4676    1802

  Fragments arranged by size: 

                4676    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4432    5321    6950 
   Size:         54    1425      50     275      18    2602     889    1629

Cuts at:   6950       8 
   Size:         43

  Fragments arranged by size: 

                2602    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4432    1537 
   Size:        275    2620    4090

  Fragments arranged by size: 

                4090    2620     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2560    2604    4915    1353 
   Size:       1207      44    2311    3423

  Fragments arranged by size: 

                3423    2311    1207      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5572    5946    6092     387 
   Size:       1064     138    3983     374     146    1280

  Fragments arranged by size: 

                3983    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2127    2156    2790    2956    3933    3940    5048 
   Size:        393     495      29     634     166     977       7    1108

Cuts at:   5048    5645    6432    6700    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1108     977     787     634     597     495     393
                 268     166      29       7
 
EcoO109I rG'GnC_Cy

Cuts at:   2831    2831 
   Size:       6985
 
EcoRI G'AATT_C

Cuts at:   4039    4294    4672    4039 
   Size:        255     378    6352

  Fragments arranged by size: 

                6352     378     255
 
EcoRV GAT'ATC

Cuts at:   3678    3678 
   Size:       6985
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2222    3625    2222 
   Size:       1403    5582
 
FspI TGC'GCA

Cuts at:   5491    5491 
   Size:       6985
 
HaeII r_GCGC'y

Cuts at:   1678    6132    1678 
   Size:       4454    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3562    3594    3667    3699    5626 
   Size:         32     135      32    1990      32      73      32    1927

Cuts at:   5626    5658    5700    5732    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1990    1927     135      73      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4329    4575     669 
   Size:        988     281    2391     246    3079

  Fragments arranged by size: 

                3079    2391     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2363     748 
   Size:       1615    5370
 
HpaI GTT'AAC

Cuts at:   4575    4575 
   Size:       6985
 
KpnI G_GTAC'C

Cuts at:   4310    4310 
   Size:       6985
 
MfeI C'AATT_G

Cuts at:   4584    4584 
   Size:       6985
 
MluI A'CGCG_T

Cuts at:   3972    6861    3972 
   Size:       2889    4096
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    6099    6283    1305 
   Size:       4794     184    2007

  Fragments arranged by size: 

                4794    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5163

  Fragments arranged by size: 

                5163    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3830    4244    4943    5302    5461     518 
   Size:        648    2664     414     699     359     159    2042

  Fragments arranged by size: 

                2664    2042     699     648     414     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3511    3812    3960    4401    5078    6226    6471    1606 
   Size:       1905     301     148     441     677    1148     245    2120

  Fragments arranged by size: 

                2120    1905    1148     677     441     301     245     148
            
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6433
 
NdeI CA'TA_TG

Cuts at:    387    2494    2671    3863     387 
   Size:       2107     177    1192    3509

  Fragments arranged by size: 

                3509    2107    1192     177
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6985
 
NotI GC'GGCC_GC

Cuts at:   4432    4432 
   Size:       6985
 
NspI r_CATG'y

Cuts at:   1132    1229    4343    5888    1132 
   Size:         97    3114    1545    2229

  Fragments arranged by size: 

                3114    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6985
 
PciI A'CATG_T

Cuts at:   5884    5884 
   Size:       6985
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2768    1108 
   Size:       1660    5325
 
PfoI T'CCnGG_A

Cuts at:   3465    3465 
   Size:       6985
 
PmeI GTTT'AAAC

Cuts at:   4289    4289 
   Size:       6985
 
PmlI CAC'GTG

Cuts at:   2895    2895 
   Size:       6985
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5054    5086    6567    6599    5054 
   Size:         32    1481      32    5440

  Fragments arranged by size: 

                5440    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2831    2831 
   Size:       6985
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6985
 
PsiI TTA'TAA

Cuts at:   2166    2241    4555    2166 
   Size:         75    2314    4596

  Fragments arranged by size: 

                4596    2314      75
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   4220    4252    4220 
   Size:         32    6953
 
PstI C_TGCA'G

Cuts at:    830    2221    3641    4337     830 
   Size:       1391    1420     696    3478

  Fragments arranged by size: 

                3478    1420    1391     696
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6985
 
PvuII CAG'CTG

Cuts at:   3511    3812    3960    3511 
   Size:        301     148    6536

  Fragments arranged by size: 

                6536     301     148
 
SalI G'TCGA_C

Cuts at:   1936    4327    1936 
   Size:       2391    4594
 
SbfI CC_TGCA'GG

Cuts at:   4337    4337 
   Size:       6985
 
ScaI AGT'ACT

Cuts at:   2235    3386    5233    6945    2235 
   Size:       1151    1847    1712    2275

  Fragments arranged by size: 

                2275    1847    1712    1151
 
SfcI C'TryA_G

Cuts at:    826    1045    2217    3277    3637    4333    5468    6149    6340 
   Size:        219    1172    1060     360     696    1135     681     191

Cuts at:   6340     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1172    1135    1060     696     681     360     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6985
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6985
 
SmaI CCC'GGG

Cuts at:   1872    4312    1872 
   Size:       2440    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2866    4025    5089    5990    6252     820 
   Size:        197     926     923    1159    1064     901     262    1553

  Fragments arranged by size: 

                1553    1159    1064     926     923     901     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4776     493 
   Size:       4283    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6985
 
SphI G_CATG'C

Cuts at:   1132    4343    1132 
   Size:       3211    3774
 
SspI AAT'ATT

Cuts at:      5      52    2245    2580    2752    3105    3353    3968    4067 
   Size:         47    2193     335     172     353     248     615      99

Cuts at:   4067    4909       5 
   Size:        842    2081

  Fragments arranged by size: 

                2193    2081     842     615     353     335     248     172
                  99      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6985
 
StyI C'CwwG_G

Cuts at:    513    1065    3714    3742    4424     513 
   Size:        552    2649      28     682    3074

  Fragments arranged by size: 

                3074    2649     682     552      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2941    3770    4256    5012    5029    5182    5367 
   Size:       1255    1361     829     486     756      17     153     185

Cuts at:   5367    5812     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1361    1255     829     756     486     445     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2233    3384    5231 
   Size:        274      80      33      51    1443     256    1151    1847

Cuts at:   5231    6943      96 
   Size:       1712     138

  Fragments arranged by size: 

                1847    1712    1443    1151     274     256     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4196    4228    6945    6977    4196 
   Size:         32    2717      32    4204

  Fragments arranged by size: 

                4204    2717      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6985
 
XbaI T'CTAG_A

Cuts at:   2344    4321    2344 
   Size:       1977    5008
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3957    3957 
   Size:       6985
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6985
 
XmaI C'CCGG_G

Cuts at:   1870    4310    1870 
   Size:       2440    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2158    2205    5114    2158 
   Size:         47    2909    4029

  Fragments arranged by size: 

                4029    2909      47
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4793     276 
   Size:         53      83     186     936    3259    2468

  Fragments arranged by size: 

                3259    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI   PspOMI    PspXI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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