Restriction map for
pFN21AB7526
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRII EcoT22I
FauI FbaI Fnu4HI FseI FspI
HapII HgaI HincII HindII HindIII
HpaI HpaII ItaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvaI MvnI NaeI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 18 GGCGCC G[GA]^CG[CT]C
AluI 369 AGCT AG^CT
AlwI 63 GATCC GGATC(4/5)
ApaI 643 GGGCCC GGGCC^C
AseI 625 ATTAAT AT^TAAT
AsnI 625 ATTAAT AT^TAAT
BanI 18 GGCGCC G^G[CT][GA]CC
BanII 643 GGGCCC G[GA]GC[CT]^C
BbeI 18 GGCGCC GGCGC^C
BmyI 643 GGGCCC G[AGT]GC[ACT]^C
BsaHI 18 GGCGCC G[GA]^CG[CT]C
BseRI 254 CTCCTC GAGGAG(10/8)
BsgI 553 GTGCAG GTGCAG(16/14)
Bsp1286I 643 GGGCCC G[AGT]GC[ACT]^C
BstAPI 598 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 153 GGATG GGATG(2/0)
BstXI 665 CCACCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 62 AGATCC [GA]^GATC[CT]
CfoI 19 GCGC GCG^C
DpnII 63 GATC ^GATC
EarI 714 GAAGAG CTCTTC(1/4)
EcoNI 409 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 280 GATATC GAT^ATC
FokI 153 GGATG GGATG(9/13)
HaeII 18 GGCGCC [GA]GCGC^[CT]
HaeIII 644 GGCC GG^CC
HhaI 19 GCGC GCG^C
Hin1I 18 GGCGCC G[GA]^CG[CT]C
HinP1I 19 GCGC G^CGC
HphI 51 GGTGA GGTGA(8/7)
KasI 18 GGCGCC G^GCGCC
Ksp632I 714 GAAGAG CTCTTC(1/4)
MboI 63 GATC ^GATC
MflI 62 AGATCC [GA]^GATC[CT]
NarI 18 GGCGCC GG^CGCC
NdeI 326 CATATG CA^TATG
NdeII 63 GATC ^GATC
PacI 394 TTAATTAA TTAAT^TAA
PleI 4 GAGTC GAGTC(4/5)
PshBI 625 ATTAAT AT^TAAT
PstI 605 CTGCAG CTGCA^G
Sau3AI 63 GATC ^GATC
SfoI 18 GGCGCC GGC^GCC
SpeI 423 ACTAGT A^CTAGT
SspI 652 AATATT AAT^ATT
TaqI 172 TCGA T^CGA
TthHB8I 172 TCGA T^CGA
XhoII 62 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 121 GAATTT [GA]^AATT[CT]
164 AAATTT
AlwNI 61 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
590 CAGTTCCTG
ApoI 121 GAATTT [GA]^AATT[CT]
164 AAATTT
AvaII 334 GGACC G^G[AT]CC
406 GGACC
BfaI 338 CTAG C^TAG
424 CTAG
BsmAI 213 GAGAC GTCTC(1/5)
420 GAGAC
BsmFI 405 GGGAC GGGAC(10/14)
502 GTCCC
DdeI 182 CTAAG C^T[ACGT]AG
569 CTCAG
DraII 333 AGGACCT [GA]G^G[ACGT]CC[CT]
405 GGGACCT
EcoO109I 333 AGGACCT [GA]G^G[ACGT]CC[CT]
405 GGGACCT
MaeI 338 CTAG C^TAG
424 CTAG
MboII 714 GAAGA GAAGA(8/7)
727 GAAGA
MslI 666 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
724 CATGAAGATG
MwoI 598 GCAACAACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
694 GCCAATGAAGC
PpuMI 333 AGGACCT [GA]G^G[AT]CC[CT]
405 GGGACCT
TfiI 80 GAATC G^A[AT]TC
558 GATTC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BsaJI BsiYI BslI BsrI Cfr13I
EcoT14I HinfI MaeIII MnlI MseI
NlaIII NlaIV Sau96I SfaNI SfcI
StyI Tru9I Tsp45I Tsp509I TspRI