(Circular) (Six-base) MAPSORT of: pFN21AE3527.seq Check: 3625 from: 1 to: 5992 pFN21AE3527 5992 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4055 5439 5707 1632 Size: 2423 1384 268 1917 Fragments arranged by size: 2423 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2516 4891 5868 1203 Size: 1313 2375 977 1327 Fragments arranged by size: 2375 1327 1313 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 4662 AlwNI CAG_nnn'CTG Cuts at: 2132 3735 5307 2132 Size: 1603 1572 2817 Fragments arranged by size: 2817 1603 1572 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2084 2615 3317 1870 Size: 73 73 68 531 702 4545 Fragments arranged by size: 4545 702 531 73 73 68 BamHI G'GATC_C Cuts at: 3028 3322 3689 3028 Size: 294 367 5331 Fragments arranged by size: 5331 367 294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2238 2485 3138 3313 Size: 325 217 627 293 158 247 653 175 Cuts at: 3313 4768 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 653 627 325 293 247 217 175 158 BanII G_rGCy'C Cuts at: 1391 2430 2721 3311 1391 Size: 1039 291 590 4072 Fragments arranged by size: 4072 1039 590 291 BbeI G_GCGC'C Cuts at: 2084 2242 2489 2084 Size: 158 247 5587 Fragments arranged by size: 5587 247 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 3100 928 Size: 971 620 581 3820 Fragments arranged by size: 3820 971 620 581 BbvCI CC'TCA_GC Cuts at: 3279 3279 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2158 2192 2383 2417 3057 3091 4183 Size: 34 221 34 191 34 640 34 1092 Cuts at: 4183 4217 1903 Size: 34 3678 Fragments arranged by size: 3678 1092 640 221 191 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2483 4603 136 Size: 107 122 71 631 605 811 2120 1525 Fragments arranged by size: 2120 1525 811 631 605 122 107 71 BglII A'GATC_T Cuts at: 3021 5987 3021 Size: 2966 3026 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2818 4053 5209 1790 Size: 1028 1235 1156 2573 Fragments arranged by size: 2573 1235 1156 1028 BmgBI CAC'GTC Cuts at: 1318 2622 1318 Size: 1304 4688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 2812 3723 Size: 416 53 83 399 24 1269 126 911 Cuts at: 3723 4681 442 Size: 958 1753 Fragments arranged by size: 1753 1269 958 911 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3187 3187 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 3133 3207 4652 1239 Size: 870 1024 74 1445 2579 Fragments arranged by size: 2579 1445 1024 870 74 Bpu10I CC'TnA_GC Cuts at: 3279 3279 Size: 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 2895 4655 882 Size: 617 507 889 1760 2219 Fragments arranged by size: 2219 1760 889 617 507 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 2239 2486 3799 Size: 53 83 186 936 548 158 247 1313 Cuts at: 3799 4181 4816 275 Size: 382 635 1451 Fragments arranged by size: 1451 1313 936 635 548 382 247 186 158 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2107 2137 1178 Size: 30 165 30 704 30 5033 Fragments arranged by size: 5033 704 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2422 3219 1186 Size: 1236 797 3959 Fragments arranged by size: 3959 1236 797 BseYI C'CCAG_C Cuts at: 2584 5195 5691 2584 Size: 2611 496 2885 Fragments arranged by size: 2885 2611 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2029 2377 3212 1163 Size: 573 293 348 835 3943 Fragments arranged by size: 3943 835 573 348 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2331 2838 3442 4203 5231 1540 Size: 275 186 330 507 604 761 1028 2301 Fragments arranged by size: 2301 1028 761 604 507 330 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2562 2946 4053 4138 5209 2370 Size: 192 384 1107 85 1071 3153 Fragments arranged by size: 3153 1107 1071 384 192 85 BsmI GAATG_Cn' Cuts at: 2981 3503 3596 2981 Size: 522 93 5377 Fragments arranged by size: 5377 522 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2834 2834 Size: 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2370 2430 2562 2721 2818 2946 3311 Size: 399 580 60 132 159 97 128 365 Cuts at: 3311 4053 4138 5209 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 399 365 159 132 128 97 85 60 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3347 844 Size: 2207 296 3489 Fragments arranged by size: 3489 2207 296 BsrBI CCG'CTC Cuts at: 1394 1823 2467 2896 3210 3879 1394 Size: 429 644 429 314 669 3507 Fragments arranged by size: 3507 669 644 429 429 314 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 2578 3118 4636 4823 1700 Size: 110 425 343 540 1518 187 2869 Fragments arranged by size: 2869 1518 540 425 343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 2219 4052 5064 2219 Size: 1833 1012 3147 Fragments arranged by size: 3147 1833 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 3067 4796 3067 Size: 1729 4263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2455 Size: 5992 BstYI r'GATC_y Cuts at: 1972 3021 3028 3057 3322 3689 4074 4091 5543 Size: 1049 7 29 265 367 385 17 1452 Cuts at: 5543 5629 5641 5987 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1049 385 367 346 265 86 29 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2157 2454 2796 513 Size: 552 1092 297 342 3709 Fragments arranged by size: 3709 1092 552 342 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2428 487 Size: 683 56 1202 4051 Fragments arranged by size: 4051 1202 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3200 3212 3237 3508 4302 4322 2944 Size: 256 12 25 271 794 20 4614 Fragments arranged by size: 4614 794 271 256 25 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2457 2475 Size: 54 1425 50 275 18 378 249 18 Cuts at: 2475 2553 2754 2844 3439 4328 5957 8 Size: 78 201 90 595 889 1629 43 Fragments arranged by size: 1629 1425 889 595 378 275 249 201 90 78 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2671 3922 1353 Size: 1318 1251 3423 Fragments arranged by size: 3423 1318 1251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2083 4579 4953 5099 387 Size: 1064 138 494 2496 374 146 1280 Fragments arranged by size: 2496 1280 1064 494 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 3133 3207 4055 4652 5439 5707 Size: 393 477 1024 74 848 597 787 268 Cuts at: 5707 1239 Size: 1524 Fragments arranged by size: 1524 1024 848 787 597 477 393 268 74 EcoO109I rG'GnC_Cy Cuts at: 2767 2767 Size: 5992 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 2084 2242 2294 2454 2474 2489 2729 5139 Size: 406 158 52 160 20 15 240 2410 Cuts at: 5139 1678 Size: 2531 Fragments arranged by size: 2531 2410 406 240 160 158 52 20 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2911 2943 3013 Size: 32 135 32 560 32 747 32 70 Cuts at: 3013 3045 3139 3171 3194 3226 4633 4665 4707 Size: 32 94 32 23 32 1407 32 42 Cuts at: 4707 4739 1373 Size: 32 2626 Fragments arranged by size: 2626 1407 747 560 135 94 70 42 32 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KasI G'GCGC_C Cuts at: 2080 2238 2485 2080 Size: 158 247 5587 Fragments arranged by size: 5587 247 158 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 2516 5868 2516 Size: 3352 2640 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5106 5290 1305 Size: 3801 184 2007 Fragments arranged by size: 3801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2477 2756 10 Size: 54 1425 343 378 267 279 3246 Fragments arranged by size: 3246 1425 378 343 279 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2339 2496 2870 3950 4309 4468 Size: 648 906 267 157 374 1080 359 159 Cuts at: 4468 518 Size: 2042 Fragments arranged by size: 2042 1080 906 648 374 359 267 159 157 MspA1I CmG'CkG Cuts at: 1606 2781 3408 4085 5233 5478 1606 Size: 1175 627 677 1148 245 2120 Fragments arranged by size: 2120 1175 1148 677 627 245 NaeI GCC'GGC Cuts at: 3120 3120 Size: 5992 NarI GG'CG_CC Cuts at: 2081 2239 2486 2081 Size: 158 247 5587 Fragments arranged by size: 5587 247 158 NcoI C'CATG_G Cuts at: 513 1065 2454 513 Size: 552 1389 4051 Fragments arranged by size: 4051 1389 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 3118 3118 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2077 3350 4895 1132 Size: 97 848 1273 1545 2229 Fragments arranged by size: 2229 1545 1273 848 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 2132 3025 1108 Size: 1024 893 4075 Fragments arranged by size: 4075 1024 893 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PstI C_TGCA'G Cuts at: 830 3344 830 Size: 2514 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SapI GCTCTTCn'nnn_ Cuts at: 2671 2671 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 2301 2301 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 3340 4475 5156 5347 826 Size: 219 2295 1135 681 191 1471 Fragments arranged by size: 2295 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2082 2240 2487 2082 Size: 158 247 5587 Fragments arranged by size: 5587 247 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 2018 2617 3319 1872 Size: 146 599 702 4545 Fragments arranged by size: 4545 702 599 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 4096 4997 5259 820 Size: 197 926 2153 901 262 1553 Fragments arranged by size: 2153 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2454 2790 3032 3288 3431 513 Size: 552 1389 336 242 256 143 3074 Fragments arranged by size: 3074 1389 552 336 256 242 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2852 2869 3194 4019 4036 4189 4374 Size: 1255 1272 17 325 825 17 153 185 Cuts at: 4374 4819 325 Size: 445 1498 Fragments arranged by size: 1498 1272 1255 825 445 325 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 4238 5950 Size: 274 80 33 51 1443 621 1640 1712 Cuts at: 5950 96 Size: 138 Fragments arranged by size: 1712 1640 1443 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2107 2139 5952 5984 2107 Size: 32 3813 32 2115 Fragments arranged by size: 3813 2115 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2303 1316 Size: 987 5005 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 2854 2767 Size: 87 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 2016 2615 3317 1870 Size: 146 599 702 4545 Fragments arranged by size: 4545 702 599 146 XmnI GAAnn'nnTTC Cuts at: 2264 4121 2264 Size: 1857 4135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI