Restriction map for
pFN21AE3521
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbrPI BbsI
BcgI BclI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI HpaI KpnI KspI MamI
MfeI MluI MroI MslI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 37 GGCGCC G[GA]^CG[CT]C
Alw44I 1032 GTGCAC G^TGCAC
ApaLI 1032 GTGCAC G^TGCAC
AvaI 236 CCCGGG C^[CT]CG[GA]G
AvrII 33 CCTAGG C^CTAGG
BalI 397 TGGCCA TGG^CCA
BbeI 37 GGCGCC GGCGC^C
BbvCI 1334 CCTCAGC CCTCAGC(-5/-2)
BciVI 297 GTATCC GTATCC(6/5)
BlnI 33 CCTAGG C^CTAGG
BsaAI 543 CACGTA [CT]AC^GT[GA]
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BsaI 1228 GAGACC GGTCTC(1/5)
BsgI 502 GTGCAG GTGCAG(16/14)
BsmBI 137 CGTCTC CGTCTC(1/5)
BsoBI 236 CCCGGG C^[CT]CG[GA]G
BspHI 317 TCATGA T^CATGA
BsrDI 551 GCAATG GCAATG(2/0)
BsrFI 468 ACCGGC [GA]^CCGG[CT]
BstXI 1062 CCAGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1064 AGATCC [GA]^GATC[CT]
Cfr10I 468 ACCGGC [GA]^CCGG[CT]
DsaI 622 CCACGG C^C[GA][CT]GG
EcoT22I 983 ATGCAT ATGCA^T
FauI 1176 GCGGG CCCGC(4/6)
HaeII 37 GGCGCC [GA]GCGC^[CT]
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HincII 713 GTCAAC GT[CT]^[GA]AC
HindII 713 GTCAAC GT[CT]^[GA]AC
HindIII 1358 AAGCTT A^AGCTT
KasI 37 GGCGCC G^GCGCC
MaeIII 862 GTCAC ^GT[ACGT]AC
MflI 1064 AGATCC [GA]^GATC[CT]
MluNI 397 TGGCCA TGG^CCA
MscI 397 TGGCCA TGG^CCA
NarI 37 GGCGCC GG^CGCC
NsiI 983 ATGCAT ATGCA^T
NspI 1083 GCATGC [GA]CATG^[CT]
PleI 931 GACTC GAGTC(4/5)
RcaI 317 TCATGA T^CATGA
SacI 90 GAGCTC GAGCT^C
SfoI 37 GGCGCC GGC^GCC
SmaI 236 CCCGGG CCC^GGG
SmlI 445 CTCAAG C^T[CT][GA]AG
SphI 1083 GCATGC GCATG^C
Tsp45I 862 GTCAC ^GT[GC]AC
XhoII 1064 AGATCC [GA]^GATC[CT]
XmaCI 236 CCCGGG C^CCGGG
XmaI 236 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 434 GTAGAC GT^[AC][GT]AC
601 GTAGAC
AspHI 90 GAGCTC G[AT]GC[AT]^C
1032 GTGCAC
BanI 37 GGCGCC G^G[CT][GA]CC
673 GGCACC
BsiHKAI 90 GAGCTC G[AT]GC[AT]^C
1032 GTGCAC
BsmI 842 GCATTC GAATGC(1/-1)
985 GCATTC
BsrI 496 CCAGT ACTGG(1/-1)
718 CCAGT
DdeI 1028 CTGAG C^T[ACGT]AG
1335 CTCAG
EcoT14I 33 CCTAGG C^C[AT][AT]GG
1068 CCTTGG
MseI 284 TTAA T^TAA
980 TTAA
MspA1I 7 CAGCTG C[AC]G^C[GT]G
1328 CAGCTG
NdeI 805 CATATG CA^TATG
1147 CATATG
PvuII 7 CAGCTG CAG^CTG
1328 CAGCTG
SapI 87 GAAGAGC GCTCTTC(1/4)
1099 GAAGAGC
StyI 33 CCTAGG C^C[AT][AT]GG
1068 CCTTGG
Tru9I 284 TTAA T^TAA
980 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI TspRI